AT4G23890

NAD(P)H dehydrogenase subunit S

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016673
Physical Location & Seq
Reverse (-)
12420414 .. 12421464
1051 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G23890.1

Sequence Viewer

Length: 753 bp
ATGGCGACTTCTTCGATCACTATCCCGACCATTAGAACTCCGATTCATCGATCAAAATTTCTAGGCCAAACACATCAATTTTCAACTGTAAACCGGTCGGTTTTCCCGCCTCCAAAACAGCAGTCAAAACTGTATCAAGTAAAGGCCATGGGTAAATTCAATCTATGGGAAGTGATGGGAGGAAGAGGATTATGCAACGGAGAGAAAGGTATTGAGAAGGAGCTCCAGAGGAACATTGAAGACGAGCAAGAGACATCAAAGGCTGAGAACAACGAGACAGAGAGAGAGAGCGATGACAGTAACTTGTCTTTTAAAGTACCTGAAGATGGTTTCGAGAAAGAGATGATGGGTCTCACAGGAGGATTTCCCGGTGGCGAAAAGGGTTTAAAAACGTTTATCGAAAAAAATCCACCACCTCCTCCACCACCACCACCTGCAAAACAAGGAAGCGATGCATCCGCGGTTGCTACAGATAAGAAGCCGAAAGCGCCCAAGCTACCCCTTCTCATGCCGGGGATGATTGCTATAGTCAAGAACCAGAACAGTCCGTATCACATGTATTGTGGAATTGTGCAGAGGATCACTGATGGAAAAGCTGGTGTCTTGTTCGAAGGAGGAAACTGGGACCGTCTCATTACTTTCAGGCTTGAAGAGCTTGAACGAAGAGAAAAAGGTCCACCGGGTAAGAATCCAAAGTCTTGTATTCTCGAGCCTCTTATCGAACAGATGCAAAAGGAAGAGGCAGCACCATAA

Protein Analysis

250

Amino Acids

27.73

Weight (kDa)

8.32

Isoelectric Point (pI)

56.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NdhS PF11623 169 - 220 4.1e-28 NAD(P)H dehydrogenase subunit S
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016673)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23890
fragaria_vesca FvH4_5g04650
prunus_persica Prupe.5G120500_v2.0.a1
pyrus_communis pycom06g10140
rosa_laevigata RLG00000004174
rosa_multiflora Rmu_sc0004154.1_g000007 Rmu_sc0008861.1_g000005
rosa_roxburghii Rroxscaffold_3G00261100
rosa_rugosa Rorug07G0017800
rosa_samantha Rh7AG145500 Rh7BG145300 Rh7CG149500 Rh7DG146800
rosa_wichuraiana Rw7G012420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 442
Acc36I ACCTGC 1 cut(s) 442
AccII CGCG 1 cut(s) 461
AciI CCGC 3 cut(s) 107, 459, 461
AclI AACGTT 1 cut(s) 392
AclWI GGATC 1 cut(s) 587
AcsI RAATTY 2 cut(s) 56, 155
AcuI CTGAAG 1 cut(s) 342
AfaI GTAC 1 cut(s) 318
AfiI CCNNNNNNNGG 1 cut(s) 326
AflIII ACRYGT 1 cut(s) 555
AgeI ACCGGT 1 cut(s) 93
AgsI TTSAA 5 cut(s) 84, 160, 239, 650, 659
AluBI AGCT 4 cut(s) 223, 496, 596, 655
AluI AGCT 4 cut(s) 223, 496, 596, 655
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 4 cut(s) 245, 269, 356, 635
AlwI GGATC 1 cut(s) 587
Ama87I CYCGRG 1 cut(s) 707
AoxI GGCC 2 cut(s) 64, 144
ApeKI GCWGC 1 cut(s) 743
ApoI RAATTY 2 cut(s) 56, 155
ArsI GACNNNNNNTTYG 2 cut(s) 107, 139
AsiGI ACCGGT 1 cut(s) 93
AspLEI GCGC 1 cut(s) 490
AspS9I GGNCC 2 cut(s) 625, 674
AsuC2I CCSGG 3 cut(s) 369, 513, 681
AsuII TTCGAA 1 cut(s) 609
AvaI CYCGRG 1 cut(s) 707
AvaII GGWCC 2 cut(s) 625, 674
BanII GRGCYC 1 cut(s) 225
BbsI GAAGAC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 225
BccI CCATC 4 cut(s) 169, 320, 340, 581
BcnI CCSGG 3 cut(s) 369, 513, 681
BcoDI GTCTC 4 cut(s) 245, 269, 356, 635
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 2 cut(s) 468, 525
BfoI RGCGCY 1 cut(s) 491
BfuAI ACCTGC 1 cut(s) 442
BisI GCNGC 1 cut(s) 744
BlsI GCNGC 1 cut(s) 745
Bme1390I CCNGG 3 cut(s) 369, 513, 681
Bme18I GGWCC 2 cut(s) 625, 674
BmeT110I CYCGRG 1 cut(s) 707
BmgT120I GGNCC 2 cut(s) 625, 674
BmiI GGNNCC 1 cut(s) 626
BmrFI CCNGG 3 cut(s) 369, 513, 681
BmrI ACTGGG 1 cut(s) 631
BmsI GCATC 3 cut(s) 442, 464, 717
BmuI ACTGGG 1 cut(s) 631
BpiI GAAGAC 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 209
Bpu14I TTCGAA 1 cut(s) 609
BpuMI CCSGG 3 cut(s) 369, 513, 681
Bsa29I ATCGAT 1 cut(s) 49
BsaBI GATNNNNATC 1 cut(s) 20
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 3 cut(s) 147, 459, 512
BsaWI WCCGGW 1 cut(s) 93
Bsc4I CCNNNNNNNGG 1 cut(s) 326
Bse118I RCCGGY 1 cut(s) 93
Bse1I ACTGG 1 cut(s) 626
Bse8I GATNNNNATC 1 cut(s) 20
BseCI ATCGAT 1 cut(s) 49
BseDI CCNNGG 3 cut(s) 147, 459, 512
BseGI GGATG 2 cut(s) 455, 522
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 1 cut(s) 326
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 1 cut(s) 626
BseRI GAGGAG 1 cut(s) 408
BsgI GTGCAG 1 cut(s) 593
Bsh1236I CGCG 1 cut(s) 461
Bsh1285I CGRYCG 1 cut(s) 98
BshFI GGCC 2 cut(s) 66, 146
BshTI ACCGGT 1 cut(s) 93
BshVI ATCGAT 1 cut(s) 49
BsiEI CGRYCG 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 707
BsiSI CCGG 4 cut(s) 94, 369, 512, 680
BslFI GGGAC 1 cut(s) 638
BslI CCNNNNNNNGG 1 cut(s) 326
BsmAI GTCTC 4 cut(s) 245, 269, 356, 635
BsmBI CGTCTC 1 cut(s) 635
BsmFI GGGAC 1 cut(s) 638
BsnI GGCC 2 cut(s) 66, 146
Bso31I GGTCTC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 707
Bsp119I TTCGAA 1 cut(s) 609
Bsp1286I GDGCHC 1 cut(s) 225
Bsp143I GATC 3 cut(s) 15, 50, 579
Bsp19I CCATGG 1 cut(s) 147
BspACI CCGC 3 cut(s) 107, 459, 461
BspANI GGCC 2 cut(s) 66, 146
BspCNI CTCAG 1 cut(s) 256
BspDI ATCGAT 1 cut(s) 49
BspFNI CGCG 1 cut(s) 461
BspLI GGNNCC 1 cut(s) 626
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 1 cut(s) 587
BspQI GCTCTTC 1 cut(s) 645
BspT104I TTCGAA 1 cut(s) 609
BspTNI GGTCTC 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 93
BsrI ACTGG 1 cut(s) 626
BssAI RCCGGY 1 cut(s) 93
BssECI CCNNGG 3 cut(s) 147, 459, 512
BssMI GATC 3 cut(s) 15, 50, 579
BssT1I CCWWGG 1 cut(s) 147
Bst4CI ACNGT 5 cut(s) 88, 132, 299, 545, 629
Bst6I CTCTTC 4 cut(s) 178, 645, 658, 732
BstBI TTCGAA 1 cut(s) 609
BstDEI CTNAG 1 cut(s) 264
BstDSI CCRYGG 2 cut(s) 147, 459
BstF5I GGATG 2 cut(s) 455, 522
BstFNI CGCG 1 cut(s) 461
BstH2I RGCGCY 1 cut(s) 491
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 3 cut(s) 18, 53, 582
BstMAI GTCTC 4 cut(s) 245, 269, 356, 635
BstMBI GATC 3 cut(s) 15, 50, 579
BstMCI CGRYCG 1 cut(s) 98
BstMWI GCNNNNNNNGC 2 cut(s) 487, 652
BstNSI RCATGY 1 cut(s) 559
BstSCI CCNGG 3 cut(s) 367, 511, 679
BstSFI CTRYAG 2 cut(s) 468, 525
BstUI CGCG 1 cut(s) 461
BstV2I GAAGAC 1 cut(s) 246
Bsu15I ATCGAT 1 cut(s) 49
BsuRI GGCC 2 cut(s) 66, 146
BsuTUI ATCGAT 1 cut(s) 49
BtgI CCRYGG 2 cut(s) 147, 459
BtgZI GCGATG 2 cut(s) 306, 465
BtsCI GGATG 2 cut(s) 455, 522
BtsIMutI CAGTG 1 cut(s) 582
BveI ACCTGC 1 cut(s) 442
CfoI GCGC 1 cut(s) 490
Cfr10I RCCGGY 1 cut(s) 93
Cfr13I GGNCC 2 cut(s) 625, 674
Cfr42I CCGCGG 1 cut(s) 462
ClaI ATCGAT 1 cut(s) 49
Csp6I GTAC 1 cut(s) 317
CspAI ACCGGT 1 cut(s) 93
CviAII CATG 3 cut(s) 148, 508, 556
CviQI GTAC 1 cut(s) 317
DdeI CTNAG 1 cut(s) 264
DpnI GATC 3 cut(s) 17, 52, 581
DpnII GATC 3 cut(s) 15, 50, 579
DraI TTTAAA 2 cut(s) 313, 387
Eam1104I CTCTTC 4 cut(s) 178, 645, 658, 732
EarI CTCTTC 4 cut(s) 178, 645, 658, 732
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 1 cut(s) 356
Eco47I GGWCC 2 cut(s) 625, 674
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 342
Eco88I CYCGRG 1 cut(s) 707
EcoICRI GAGCTC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 147
EcoT22I ATGCAT 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 147
Esp3I CGTCTC 1 cut(s) 635
FaeI CATG 3 cut(s) 151, 511, 559
FaiI YATR 7 cut(s) 149, 166, 193, 509, 527, 557, 751
FaqI GGGAC 1 cut(s) 638
FatI CATG 3 cut(s) 147, 507, 555
FauI CCCGC 1 cut(s) 114
Fnu4HI GCNGC 1 cut(s) 744
FokI GGATG 2 cut(s) 442, 529
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 744
FspBI CTAG 1 cut(s) 62
GlaI GCGC 1 cut(s) 489
GluI GCNGC 1 cut(s) 744
GsuI CTGGAG 1 cut(s) 209
HaeII RGCGCY 1 cut(s) 491
HaeIII GGCC 2 cut(s) 66, 146
HapII CCGG 4 cut(s) 94, 369, 512, 680
HhaI GCGC 1 cut(s) 490
Hin1II CATG 3 cut(s) 151, 511, 559
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HinfI GANTC 2 cut(s) 43, 688
HpaII CCGG 4 cut(s) 94, 369, 512, 680
Hpy166II GTNNAC 2 cut(s) 91, 677
Hpy188I TCNGA 1 cut(s) 42
Hpy188III TCNNGA 5 cut(s) 25, 226, 334, 532, 707
Hpy8I GTNNAC 2 cut(s) 91, 677
HpyAV CCTTC 3 cut(s) 211, 512, 605
HpyCH4III ACNGT 5 cut(s) 88, 132, 299, 545, 629
HpyCH4IV ACGT 1 cut(s) 392
HpyCH4V TGCA 5 cut(s) 195, 437, 455, 574, 730
HpyF10VI GCNNNNNNNGC 2 cut(s) 487, 652
HpyF3I CTNAG 1 cut(s) 264
HpySE526I ACGT 1 cut(s) 392
Hsp92II CATG 3 cut(s) 151, 511, 559
HspAI GCGC 1 cut(s) 488
KspI CCGCGG 1 cut(s) 462
Kzo9I GATC 3 cut(s) 15, 50, 579
LguI GCTCTTC 1 cut(s) 645
LmnI GCTCC 2 cut(s) 220, 228
LweI GCATC 3 cut(s) 442, 464, 717
MaeI CTAG 1 cut(s) 62
MaeII ACGT 1 cut(s) 392
MaeIII GTNAC 1 cut(s) 299
MalI GATC 3 cut(s) 17, 52, 581
MboI GATC 3 cut(s) 15, 50, 579
MboII GAAGA 7 cut(s) 3, 195, 251, 335, 662, 675, 749
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 4 cut(s) 56, 77, 155, 567
Mph1103I ATGCAT 1 cut(s) 457
MseI TTAA 2 cut(s) 312, 386
MspA1I CMGCKG 1 cut(s) 461
MspI CCGG 4 cut(s) 94, 369, 512, 680
MspR9I CCNGG 3 cut(s) 369, 513, 681
MvnI CGCG 1 cut(s) 461
MwoI GCNNNNNNNGC 2 cut(s) 487, 652
NciI CCSGG 3 cut(s) 369, 513, 681
NcoI CCATGG 1 cut(s) 147
NdeII GATC 3 cut(s) 15, 50, 579
NlaIII CATG 3 cut(s) 151, 511, 559
NlaIV GGNNCC 1 cut(s) 626
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 1 cut(s) 559
NspV TTCGAA 1 cut(s) 609
PaeR7I CTCGAG 1 cut(s) 707
PaqCI CACCTGC 1 cut(s) 442
PciI ACATGT 1 cut(s) 555
PciSI GCTCTTC 1 cut(s) 645
PfeI GAWTC 2 cut(s) 43, 688
PinAI ACCGGT 1 cut(s) 93
PkrI GCNGC 1 cut(s) 745
PscI ACATGT 1 cut(s) 555
Psp124BI GAGCTC 1 cut(s) 225
Psp1406I AACGTT 1 cut(s) 392
PspN4I GGNNCC 1 cut(s) 626
PspPI GGNCC 2 cut(s) 625, 674
RsaI GTAC 1 cut(s) 318
RsaNI GTAC 1 cut(s) 317
SacI GAGCTC 1 cut(s) 225
SacII CCGCGG 1 cut(s) 462
SapI GCTCTTC 1 cut(s) 645
SaqAI TTAA 2 cut(s) 312, 386
SatI GCNGC 1 cut(s) 744
Sau3AI GATC 3 cut(s) 15, 50, 579
Sau96I GGNCC 2 cut(s) 625, 674
ScrFI CCNGG 3 cut(s) 369, 513, 681
SduI GDGCHC 1 cut(s) 225
SfaNI GCATC 3 cut(s) 442, 464, 717
SfcI CTRYAG 2 cut(s) 468, 525
Sfr274I CTCGAG 1 cut(s) 707
Sfr303I CCGCGG 1 cut(s) 462
SfuI TTCGAA 1 cut(s) 609
SgrBI CCGCGG 1 cut(s) 462
SinI GGWCC 2 cut(s) 625, 674
SlaI CTCGAG 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 707
SmoI CTYRAG 1 cut(s) 707
Sse9I AATT 4 cut(s) 56, 77, 155, 567
SsiI CCGC 3 cut(s) 107, 459, 461
SspMI CTAG 1 cut(s) 62
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 3 cut(s) 367, 511, 679
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 5 cut(s) 88, 132, 299, 545, 629
TaiI ACGT 1 cut(s) 395
TaqI TCGA 7 cut(s) 14, 49, 333, 399, 609, 708, 720
TasI AATT 4 cut(s) 56, 77, 155, 567
TfiI GAWTC 2 cut(s) 43, 688
Tru1I TTAA 2 cut(s) 312, 386
Tru9I TTAA 2 cut(s) 312, 386
TscAI CASTG 1 cut(s) 589
TseI GCWGC 1 cut(s) 743
TspDTI ATGAA 1 cut(s) 35
TspGWI ACGGA 2 cut(s) 213, 537
TspRI CASTG 1 cut(s) 589
VpaK11BI GGWCC 2 cut(s) 625, 674
XapI RAATTY 2 cut(s) 56, 155
XceI RCATGY 1 cut(s) 559
XhoI CTCGAG 1 cut(s) 707
XspI CTAG 1 cut(s) 62
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.