Rh7BG145300

NAD(P)H dehydrogenase subunit S

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0016673
Physical Location & Seq
Forward (+)
10754166 .. 10754897
732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG145300.1

Sequence Viewer

Length: 732 bp
ATGGCTTCCAACATCAGTCTCCCTACCTTTCAAGGCCCTCTTCTAAAATCCAATTTCCTAGGAAGAAATGACCTCTCTAATCTGGCCCCCAAGCCTTCTTTCACAGTTCAAAGAAGGCCATCCACCCATCACCTAAGAACATATGCCAAGTTTAATCTATTCGAAATCTTGGGAGGCAGAGGGCTCTTAAATGGTGAGCGAGGTGTTGAGAAAGAGCTTACAAGAAACGTCGAAGAACAAGCACCAGCAGCTGCTGGTGATGAAGCAGAGAAAACAGAGGGAGAAGAAGGTTCGGGAATTACTGCCATTGACAGTGCTCAAGAAGCTGCTTTTGAAAAGGAGATGATGGGGCTTACTGGGGGATTTCCTGGTGGCGAAACGGGGTTGCAAAAGTTCATTGAGAAAAACCCACCTCCCAAGAAACCACCAGCTGCAGACTCTGGAACAACAGCAGCGCTTGTGAGCTCAAAGAAGCCAAAAGCACCTGAGTTGCCATTGTTACTACCTGGTATGATCGCCATAGTGAGCAATCCAAATAACCCATATCACATGTACTGTGGGATTGTTCAGAGAATCACTGATGGAAAAGCTGGGGTTCTTTTTGAGGGAGGAAACTGGGATCGGTTAATCACTTTTCGACTAGACGAGCTAACTCGCAGAGAGAAGGGGCCTCCAGGGAAGAACCCCAGATCCGCGATTCTTGAACCGCTCCTTAAAAAGGATTCTGAATGA

Protein Analysis

243

Amino Acids

26.24

Weight (kDa)

7.8

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NdhS PF11623 167 - 218 1.2e-27 NAD(P)H dehydrogenase subunit S
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0016673)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23890
fragaria_vesca FvH4_5g04650
prunus_persica Prupe.5G120500_v2.0.a1
pyrus_communis pycom06g10140
rosa_laevigata RLG00000004174
rosa_multiflora Rmu_sc0004154.1_g000007 Rmu_sc0008861.1_g000005
rosa_roxburghii Rroxscaffold_3G00261100
rosa_rugosa Rorug07G0017800
rosa_samantha Rh7AG145500 Rh7BG145300 Rh7CG149500 Rh7DG146800
rosa_wichuraiana Rw7G012420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 709
AccII CGCG 1 cut(s) 695
AciI CCGC 2 cut(s) 693, 707
AclWI GGATC 2 cut(s) 627, 684
AfaI GTAC 1 cut(s) 554
AfeI AGCGCT 1 cut(s) 456
AfiI CCNNNNNNNGG 1 cut(s) 718
AflIII ACRYGT 1 cut(s) 549
AgsI TTSAA 4 cut(s) 32, 110, 335, 704
AjnI CCWGG 3 cut(s) 367, 505, 673
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AloI GAACNNNNNNTCC 2 cut(s) 674, 706
AluBI AGCT 7 cut(s) 217, 251, 326, 431, 465, 590, 649
AluI AGCT 7 cut(s) 217, 251, 326, 431, 465, 590, 649
Alw21I GWGCWC 2 cut(s) 319, 467
Alw26I GTCTC 1 cut(s) 23
AlwI GGATC 2 cut(s) 627, 684
AlwNI CAGNNNCTG 3 cut(s) 251, 254, 440
Aor51HI AGCGCT 1 cut(s) 456
AoxI GGCC 4 cut(s) 34, 84, 116, 668
ApeKI GCWGC 5 cut(s) 248, 251, 326, 431, 452
AspA2I CCTAGG 1 cut(s) 58
AspLEI GCGC 1 cut(s) 457
AspS9I GGNCC 3 cut(s) 35, 85, 668
AsuHPI GGTGA 3 cut(s) 122, 206, 269
AsuII TTCGAA 1 cut(s) 162
AvrII CCTAGG 1 cut(s) 58
BanII GRGCYC 2 cut(s) 186, 467
Bbv12I GWGCWC 2 cut(s) 319, 467
BbvI GCAGC 5 cut(s) 238, 260, 313, 418, 464
BccI CCATC 4 cut(s) 127, 135, 340, 575
BciT130I CCWGG 3 cut(s) 369, 507, 675
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 2 cut(s) 59, 641
BfmI CTRYAG 1 cut(s) 432
BfoI RGCGCY 1 cut(s) 458
BisI GCNGC 5 cut(s) 249, 252, 327, 432, 453
BlnI CCTAGG 1 cut(s) 58
BlsI GCNGC 5 cut(s) 250, 253, 328, 433, 454
Bme1390I CCNGG 3 cut(s) 369, 507, 675
BmgT120I GGNCC 3 cut(s) 35, 85, 668
BmiI GGNNCC 2 cut(s) 87, 669
BmrFI CCNGG 3 cut(s) 369, 507, 675
BmrI ACTGGG 2 cut(s) 366, 625
BmuI ACTGGG 2 cut(s) 366, 625
BpmI CTGGAG 1 cut(s) 657
Bpu14I TTCGAA 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 303
BsaJI CCNNGG 2 cut(s) 58, 674
Bsc4I CCNNNNNNNGG 1 cut(s) 718
Bse1I ACTGG 2 cut(s) 361, 620
BseBI CCWGG 3 cut(s) 369, 507, 675
BseDI CCNNGG 2 cut(s) 58, 674
BseGI GGATG 1 cut(s) 119
BseLI CCNNNNNNNGG 1 cut(s) 718
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 2 cut(s) 361, 620
BseXI GCAGC 5 cut(s) 238, 260, 313, 418, 464
BseYI CCCAGC 1 cut(s) 590
Bsh1236I CGCG 1 cut(s) 695
BshFI GGCC 4 cut(s) 36, 86, 118, 670
BsiHKAI GWGCWC 2 cut(s) 319, 467
BslI CCNNNNNNNGG 1 cut(s) 718
BsmAI GTCTC 1 cut(s) 23
BsnI GGCC 4 cut(s) 36, 86, 118, 670
Bsp119I TTCGAA 1 cut(s) 162
Bsp1286I GDGCHC 3 cut(s) 186, 319, 467
Bsp143I GATC 3 cut(s) 513, 619, 689
BspACI CCGC 2 cut(s) 693, 707
BspANI GGCC 4 cut(s) 36, 86, 118, 670
BspCNI CTCAG 1 cut(s) 478
BspFNI CGCG 1 cut(s) 695
BspLI GGNNCC 2 cut(s) 87, 669
BspMAI CTGCAG 1 cut(s) 436
BspPI GGATC 2 cut(s) 627, 684
BspT104I TTCGAA 1 cut(s) 162
BsrBI CCGCTC 1 cut(s) 709
BsrI ACTGG 2 cut(s) 361, 620
BssECI CCNNGG 2 cut(s) 58, 674
BssMI GATC 3 cut(s) 513, 619, 689
BssT1I CCWWGG 1 cut(s) 58
Bst2UI CCWGG 3 cut(s) 369, 507, 675
Bst4CI ACNGT 3 cut(s) 106, 314, 557
Bst6I CTCTTC 1 cut(s) 45
BstBI TTCGAA 1 cut(s) 162
BstDEI CTNAG 2 cut(s) 134, 486
BstENI CCTNNNNNAGG 1 cut(s) 716
BstF5I GGATG 1 cut(s) 119
BstFNI CGCG 1 cut(s) 695
BstH2I RGCGCY 1 cut(s) 458
BstHHI GCGC 1 cut(s) 457
BstKTI GATC 3 cut(s) 516, 622, 692
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 3 cut(s) 513, 619, 689
BstMWI GCNNNNNNNGC 2 cut(s) 248, 323
BstNI CCWGG 3 cut(s) 369, 507, 675
BstNSI RCATGY 1 cut(s) 553
BstSCI CCNGG 3 cut(s) 367, 505, 673
BstSFI CTRYAG 1 cut(s) 432
BstUI CGCG 1 cut(s) 695
BstV1I GCAGC 5 cut(s) 238, 260, 313, 418, 464
BstX2I RGATCY 1 cut(s) 689
BstYI RGATCY 1 cut(s) 689
BsuRI GGCC 4 cut(s) 36, 86, 118, 670
BtsCI GGATG 1 cut(s) 119
BtsIMutI CAGTG 2 cut(s) 319, 576
CaiI CAGNNNCTG 3 cut(s) 251, 254, 440
CfoI GCGC 1 cut(s) 457
Cfr13I GGNCC 3 cut(s) 35, 85, 668
CsiI ACCWGGT 1 cut(s) 505
Csp6I GTAC 1 cut(s) 553
CviAII CATG 1 cut(s) 550
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 2 cut(s) 134, 486
DpnI GATC 3 cut(s) 515, 621, 691
DpnII GATC 3 cut(s) 513, 619, 689
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Ecl136II GAGCTC 1 cut(s) 465
Eco130I CCWWGG 1 cut(s) 58
Eco24I GRGCYC 2 cut(s) 186, 467
Eco47III AGCGCT 1 cut(s) 456
Eco53kI GAGCTC 1 cut(s) 465
EcoICRI GAGCTC 1 cut(s) 465
EcoNI CCTNNNNNAGG 1 cut(s) 716
EcoO109I RGGNCCY 2 cut(s) 35, 668
EcoRII CCWGG 3 cut(s) 367, 505, 673
EcoT14I CCWWGG 1 cut(s) 58
EcoT38I GRGCYC 2 cut(s) 186, 467
ErhI CCWWGG 1 cut(s) 58
FaeI CATG 1 cut(s) 553
FaiI YATR 6 cut(s) 142, 144, 512, 521, 544, 551
FalI AAGNNNNNCTT 2 cut(s) 24, 56
FatI CATG 1 cut(s) 549
FauNDI CATATG 1 cut(s) 142
Fnu4HI GCNGC 5 cut(s) 249, 252, 327, 432, 453
FokI GGATG 1 cut(s) 106
FriOI GRGCYC 2 cut(s) 186, 467
Fsp4HI GCNGC 5 cut(s) 249, 252, 327, 432, 453
FspBI CTAG 2 cut(s) 59, 641
GlaI GCGC 1 cut(s) 456
GluI GCNGC 5 cut(s) 249, 252, 327, 432, 453
GsaI CCCAGC 1 cut(s) 594
GsuI CTGGAG 1 cut(s) 657
HaeII RGCGCY 1 cut(s) 458
HaeIII GGCC 4 cut(s) 36, 86, 118, 670
HhaI GCGC 1 cut(s) 457
Hin1II CATG 1 cut(s) 553
Hin6I GCGC 1 cut(s) 455
HinP1I GCGC 1 cut(s) 455
HinfI GANTC 4 cut(s) 437, 573, 697, 722
HphI GGTGA 3 cut(s) 122, 206, 269
Hpy188I TCNGA 2 cut(s) 570, 727
Hpy188III TCNNGA 4 cut(s) 294, 320, 441, 701
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 4 cut(s) 105, 108, 281, 658
HpyCH4III ACNGT 3 cut(s) 106, 314, 557
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 2 cut(s) 388, 434
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 323
HpyF3I CTNAG 2 cut(s) 134, 486
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 1 cut(s) 553
HspAI GCGC 1 cut(s) 455
Kzo9I GATC 3 cut(s) 513, 619, 689
LmnI GCTCC 1 cut(s) 714
Lsp1109I GCAGC 5 cut(s) 238, 260, 313, 418, 464
MabI ACCWGGT 1 cut(s) 505
MaeI CTAG 2 cut(s) 59, 641
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 1 cut(s) 498
MalI GATC 3 cut(s) 515, 621, 691
MbiI CCGCTC 1 cut(s) 709
MboI GATC 3 cut(s) 513, 619, 689
MboII GAAGA 5 cut(s) 32, 75, 245, 296, 691
MflI RGATCY 1 cut(s) 689
MhlI GDGCHC 3 cut(s) 186, 319, 467
MluCI AATT 2 cut(s) 52, 297
MlyI GAGTC 1 cut(s) 431
MmeI TCCRAC 1 cut(s) 33
MseI TTAA 4 cut(s) 153, 188, 626, 714
MspA1I CMGCKG 2 cut(s) 251, 431
MspR9I CCNGG 3 cut(s) 369, 507, 675
MvaI CCWGG 3 cut(s) 369, 507, 675
MvnI CGCG 1 cut(s) 695
MwoI GCNNNNNNNGC 2 cut(s) 248, 323
NdeI CATATG 1 cut(s) 142
NdeII GATC 3 cut(s) 513, 619, 689
NlaIII CATG 1 cut(s) 553
NlaIV GGNNCC 2 cut(s) 87, 669
NspI RCATGY 1 cut(s) 553
NspV TTCGAA 1 cut(s) 162
PciI ACATGT 1 cut(s) 549
PfeI GAWTC 3 cut(s) 573, 697, 722
PkrI GCNGC 5 cut(s) 250, 253, 328, 433, 454
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
PscI ACATGT 1 cut(s) 549
Psp124BI GAGCTC 1 cut(s) 467
Psp6I CCWGG 3 cut(s) 367, 505, 673
PspFI CCCAGC 1 cut(s) 590
PspGI CCWGG 3 cut(s) 367, 505, 673
PspN4I GGNNCC 2 cut(s) 87, 669
PspPI GGNCC 3 cut(s) 35, 85, 668
PstI CTGCAG 1 cut(s) 436
PstNI CAGNNNCTG 3 cut(s) 251, 254, 440
PsuI RGATCY 1 cut(s) 689
PvuII CAGCTG 2 cut(s) 251, 431
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
SacI GAGCTC 1 cut(s) 467
SaqAI TTAA 4 cut(s) 153, 188, 626, 714
SatI GCNGC 5 cut(s) 249, 252, 327, 432, 453
Sau3AI GATC 3 cut(s) 513, 619, 689
Sau96I GGNCC 3 cut(s) 35, 85, 668
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 3 cut(s) 369, 507, 675
SduI GDGCHC 3 cut(s) 186, 319, 467
SexAI ACCWGGT 1 cut(s) 505
SfcI CTRYAG 1 cut(s) 432
SfuI TTCGAA 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 318
SmoI CTYRAG 1 cut(s) 318
Sse9I AATT 2 cut(s) 52, 297
SsiI CCGC 2 cut(s) 693, 707
SspMI CTAG 2 cut(s) 59, 641
SstI GAGCTC 1 cut(s) 467
StyD4I CCNGG 3 cut(s) 367, 505, 673
StyI CCWWGG 1 cut(s) 58
TaaI ACNGT 3 cut(s) 106, 314, 557
TaiI ACGT 1 cut(s) 231
TaqI TCGA 3 cut(s) 162, 231, 637
TasI AATT 2 cut(s) 52, 297
TatI WGTACW 1 cut(s) 552
TfiI GAWTC 3 cut(s) 573, 697, 722
Tru1I TTAA 4 cut(s) 153, 188, 626, 714
Tru9I TTAA 4 cut(s) 153, 188, 626, 714
TscAI CASTG 2 cut(s) 319, 583
TseI GCWGC 5 cut(s) 248, 251, 326, 431, 452
TspDTI ATGAA 2 cut(s) 276, 385
TspRI CASTG 2 cut(s) 319, 583
XagI CCTNNNNNAGG 1 cut(s) 716
XceI RCATGY 1 cut(s) 553
XmaJI CCTAGG 1 cut(s) 58
XspI CTAG 2 cut(s) 59, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.