Rh7CG149500

NAD(P)H dehydrogenase subunit S

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0016673
Physical Location & Seq
Forward (+)
12194548 .. 12195288
741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG149500.1

Sequence Viewer

Length: 741 bp
ATGGCTTCCAACATCAGTCTCCCTAGCTTTCAAAGCCCTCTTCTAAAATCCAATTTCCTAGGAAGAAATGACCTCTCTAATCTGGCCCCCAAGCCTTCTTTCACAGTTCAAAGAAGGCCATCCACCCATCACCTGAGAACATATGCCAAGTTTAATCTATTCGAAATCTTGGGAGGCAGAGGGCTCTTAAATGGTGAGCGAGGTGTTGAGAAAGAGCTTACAAGAAACGTCGAAGAACAAGCACCAGCAGCTGCTGGTGATGAAGCAGAGAAAACAGAGGGAGAAGAAGAATCAGAAGGTTCGGCAATTACTGCCATTGACGGTGCTCAAGAAGCTGCATTTGAAAAGGAGATGATGGGGCTTACTGGGGGATTTCCTGGTGGCGAAACGGGGTTGCAAAAGTTCATTGAGAAAAACCCACCTCCCAAGAAACCACCAGCTGCAGACTCTGGAAAAACAGCAGCGCTTGTGAGCTCAAAGAAGCCAAAAGCACCTGAGTTGCCATTGTTACTACCTGGTATGATCGCCATAGTGAGCAATCCAAATAACCCATATCACATGTACTGTGGGATTGTACAGAGAATCACTGATGGAAAAGCTGGGGTTCTTTTTGAGGGAGGAAACTGGGATCGGTTAATCACTTTTCGACTAGATGAGCTAACTCGCAGAGAGAAGGGACCACCAGGGAAGAACCCCAGATCCGCGATCCTTGAACCGCTCCTTAAAAAGGATTCTGAATGA

Protein Analysis

246

Amino Acids

26.62

Weight (kDa)

6.87

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NdhS PF11623 170 - 221 1.2e-27 NAD(P)H dehydrogenase subunit S
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0016673)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23890
fragaria_vesca FvH4_5g04650
prunus_persica Prupe.5G120500_v2.0.a1
pyrus_communis pycom06g10140
rosa_laevigata RLG00000004174
rosa_multiflora Rmu_sc0004154.1_g000007 Rmu_sc0008861.1_g000005
rosa_roxburghii Rroxscaffold_3G00261100
rosa_rugosa Rorug07G0017800
rosa_samantha Rh7AG145500 Rh7BG145300 Rh7CG149500 Rh7DG146800
rosa_wichuraiana Rw7G012420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 718
AccII CGCG 1 cut(s) 704
AciI CCGC 2 cut(s) 702, 716
AclWI GGATC 3 cut(s) 636, 693, 700
AfaI GTAC 2 cut(s) 563, 576
AfeI AGCGCT 1 cut(s) 465
AfiI CCNNNNNNNGG 1 cut(s) 727
AflIII ACRYGT 1 cut(s) 558
AgsI TTSAA 4 cut(s) 32, 110, 344, 713
AjnI CCWGG 3 cut(s) 376, 514, 682
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AloI GAACNNNNNNTCC 2 cut(s) 683, 715
AluBI AGCT 8 cut(s) 27, 217, 251, 335, 440, 474, 599, 658
AluI AGCT 8 cut(s) 27, 217, 251, 335, 440, 474, 599, 658
Alw21I GWGCWC 2 cut(s) 328, 476
Alw26I GTCTC 1 cut(s) 23
AlwI GGATC 3 cut(s) 636, 693, 700
AlwNI CAGNNNCTG 3 cut(s) 251, 254, 449
Aor51HI AGCGCT 1 cut(s) 465
AoxI GGCC 2 cut(s) 84, 116
ApeKI GCWGC 5 cut(s) 248, 251, 335, 440, 461
AspA2I CCTAGG 1 cut(s) 58
AspLEI GCGC 1 cut(s) 466
AspS9I GGNCC 2 cut(s) 85, 677
AsuHPI GGTGA 3 cut(s) 122, 206, 269
AsuII TTCGAA 1 cut(s) 162
AvaII GGWCC 1 cut(s) 677
AvrII CCTAGG 1 cut(s) 58
BanII GRGCYC 2 cut(s) 186, 476
Bbv12I GWGCWC 2 cut(s) 328, 476
BbvI GCAGC 5 cut(s) 238, 260, 322, 427, 473
BccI CCATC 4 cut(s) 127, 135, 349, 584
BciT130I CCWGG 3 cut(s) 378, 516, 684
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 3 cut(s) 24, 59, 650
BfmI CTRYAG 1 cut(s) 441
BfoI RGCGCY 1 cut(s) 467
BisI GCNGC 5 cut(s) 249, 252, 336, 441, 462
BlnI CCTAGG 1 cut(s) 58
BlsI GCNGC 5 cut(s) 250, 253, 337, 442, 463
Bme1390I CCNGG 3 cut(s) 378, 516, 684
Bme18I GGWCC 1 cut(s) 677
BmgT120I GGNCC 2 cut(s) 85, 677
BmiI GGNNCC 2 cut(s) 87, 678
BmrFI CCNGG 3 cut(s) 378, 516, 684
BmrI ACTGGG 2 cut(s) 375, 634
BmuI ACTGGG 2 cut(s) 375, 634
Bpu14I TTCGAA 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 312
BsaJI CCNNGG 2 cut(s) 58, 683
Bsc4I CCNNNNNNNGG 1 cut(s) 727
Bse1I ACTGG 2 cut(s) 370, 629
BseBI CCWGG 3 cut(s) 378, 516, 684
BseDI CCNNGG 2 cut(s) 58, 683
BseGI GGATG 1 cut(s) 119
BseLI CCNNNNNNNGG 1 cut(s) 727
BseMII CTCAG 2 cut(s) 125, 486
BseNI ACTGG 2 cut(s) 370, 629
BseXI GCAGC 5 cut(s) 238, 260, 322, 427, 473
BseYI CCCAGC 1 cut(s) 599
Bsh1236I CGCG 1 cut(s) 704
BshFI GGCC 2 cut(s) 86, 118
BsiHKAI GWGCWC 2 cut(s) 328, 476
BslFI GGGAC 1 cut(s) 690
BslI CCNNNNNNNGG 1 cut(s) 727
BsmAI GTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 690
BsnI GGCC 2 cut(s) 86, 118
Bsp119I TTCGAA 1 cut(s) 162
Bsp1286I GDGCHC 3 cut(s) 186, 328, 476
Bsp1407I TGTACA 1 cut(s) 574
Bsp143I GATC 4 cut(s) 522, 628, 698, 705
BspACI CCGC 2 cut(s) 702, 716
BspANI GGCC 2 cut(s) 86, 118
BspCNI CTCAG 2 cut(s) 126, 487
BspFNI CGCG 1 cut(s) 704
BspLI GGNNCC 2 cut(s) 87, 678
BspMAI CTGCAG 1 cut(s) 445
BspPI GGATC 3 cut(s) 636, 693, 700
BspT104I TTCGAA 1 cut(s) 162
BsrBI CCGCTC 1 cut(s) 718
BsrGI TGTACA 1 cut(s) 574
BsrI ACTGG 2 cut(s) 370, 629
BssECI CCNNGG 2 cut(s) 58, 683
BssMI GATC 4 cut(s) 522, 628, 698, 705
BssT1I CCWWGG 1 cut(s) 58
Bst2UI CCWGG 3 cut(s) 378, 516, 684
Bst4CI ACNGT 3 cut(s) 106, 323, 566
Bst6I CTCTTC 1 cut(s) 45
BstAPI GCANNNNNTGC 1 cut(s) 311
BstAUI TGTACA 1 cut(s) 574
BstBI TTCGAA 1 cut(s) 162
BstDEI CTNAG 2 cut(s) 134, 495
BstENI CCTNNNNNAGG 1 cut(s) 725
BstF5I GGATG 1 cut(s) 119
BstFNI CGCG 1 cut(s) 704
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 4 cut(s) 525, 631, 701, 708
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 4 cut(s) 522, 628, 698, 705
BstMWI GCNNNNNNNGC 4 cut(s) 33, 248, 311, 332
BstNI CCWGG 3 cut(s) 378, 516, 684
BstNSI RCATGY 1 cut(s) 562
BstSCI CCNGG 3 cut(s) 376, 514, 682
BstSFI CTRYAG 1 cut(s) 441
BstUI CGCG 1 cut(s) 704
BstV1I GCAGC 5 cut(s) 238, 260, 322, 427, 473
BstX2I RGATCY 1 cut(s) 698
BstYI RGATCY 1 cut(s) 698
BsuRI GGCC 2 cut(s) 86, 118
BtsCI GGATG 1 cut(s) 119
BtsIMutI CAGTG 1 cut(s) 585
CaiI CAGNNNCTG 3 cut(s) 251, 254, 449
CfoI GCGC 1 cut(s) 466
Cfr13I GGNCC 2 cut(s) 85, 677
CsiI ACCWGGT 1 cut(s) 514
Csp6I GTAC 2 cut(s) 562, 575
CviAII CATG 1 cut(s) 559
CviQI GTAC 2 cut(s) 562, 575
DdeI CTNAG 2 cut(s) 134, 495
DpnI GATC 4 cut(s) 524, 630, 700, 707
DpnII GATC 4 cut(s) 522, 628, 698, 705
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Ecl136II GAGCTC 1 cut(s) 474
Eco130I CCWWGG 1 cut(s) 58
Eco24I GRGCYC 2 cut(s) 186, 476
Eco47I GGWCC 1 cut(s) 677
Eco47III AGCGCT 1 cut(s) 465
Eco53kI GAGCTC 1 cut(s) 474
EcoICRI GAGCTC 1 cut(s) 474
EcoNI CCTNNNNNAGG 1 cut(s) 725
EcoRII CCWGG 3 cut(s) 376, 514, 682
EcoT14I CCWWGG 1 cut(s) 58
EcoT38I GRGCYC 2 cut(s) 186, 476
ErhI CCWWGG 1 cut(s) 58
FaeI CATG 1 cut(s) 562
FaiI YATR 6 cut(s) 142, 144, 521, 530, 553, 560
FaqI GGGAC 1 cut(s) 690
FatI CATG 1 cut(s) 558
FauNDI CATATG 1 cut(s) 142
Fnu4HI GCNGC 5 cut(s) 249, 252, 336, 441, 462
FokI GGATG 1 cut(s) 106
FriOI GRGCYC 2 cut(s) 186, 476
Fsp4HI GCNGC 5 cut(s) 249, 252, 336, 441, 462
FspBI CTAG 3 cut(s) 24, 59, 650
GlaI GCGC 1 cut(s) 465
GluI GCNGC 5 cut(s) 249, 252, 336, 441, 462
GsaI CCCAGC 1 cut(s) 603
HaeII RGCGCY 1 cut(s) 467
HaeIII GGCC 2 cut(s) 86, 118
HhaI GCGC 1 cut(s) 466
Hin1II CATG 1 cut(s) 562
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HinfI GANTC 4 cut(s) 290, 446, 582, 731
HphI GGTGA 3 cut(s) 122, 206, 269
Hpy188I TCNGA 2 cut(s) 295, 736
Hpy188III TCNNGA 2 cut(s) 329, 450
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 4 cut(s) 105, 108, 290, 667
HpyCH4III ACNGT 3 cut(s) 106, 323, 566
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 3 cut(s) 338, 397, 443
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 248, 311, 332
HpyF3I CTNAG 2 cut(s) 134, 495
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 1 cut(s) 562
HspAI GCGC 1 cut(s) 464
Kzo9I GATC 4 cut(s) 522, 628, 698, 705
LmnI GCTCC 1 cut(s) 723
Lsp1109I GCAGC 5 cut(s) 238, 260, 322, 427, 473
MabI ACCWGGT 1 cut(s) 514
MaeI CTAG 3 cut(s) 24, 59, 650
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 1 cut(s) 507
MalI GATC 4 cut(s) 524, 630, 700, 707
MbiI CCGCTC 1 cut(s) 718
MboI GATC 4 cut(s) 522, 628, 698, 705
MboII GAAGA 6 cut(s) 32, 75, 245, 296, 299, 700
MflI RGATCY 1 cut(s) 698
MhlI GDGCHC 3 cut(s) 186, 328, 476
MluCI AATT 2 cut(s) 52, 306
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 9 cut(s) 48, 83, 167, 173, 194, 271, 432, 607, 611
MseI TTAA 4 cut(s) 153, 188, 635, 723
MspA1I CMGCKG 2 cut(s) 251, 440
MspR9I CCNGG 3 cut(s) 378, 516, 684
MvaI CCWGG 3 cut(s) 378, 516, 684
MvnI CGCG 1 cut(s) 704
MwoI GCNNNNNNNGC 4 cut(s) 33, 248, 311, 332
NdeI CATATG 1 cut(s) 142
NdeII GATC 4 cut(s) 522, 628, 698, 705
NlaIII CATG 1 cut(s) 562
NlaIV GGNNCC 2 cut(s) 87, 678
NspI RCATGY 1 cut(s) 562
NspV TTCGAA 1 cut(s) 162
PciI ACATGT 1 cut(s) 558
PfeI GAWTC 3 cut(s) 290, 582, 731
PkrI GCNGC 5 cut(s) 250, 253, 337, 442, 463
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
PscI ACATGT 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 476
Psp6I CCWGG 3 cut(s) 376, 514, 682
PspFI CCCAGC 1 cut(s) 599
PspGI CCWGG 3 cut(s) 376, 514, 682
PspN4I GGNNCC 2 cut(s) 87, 678
PspPI GGNCC 2 cut(s) 85, 677
PstI CTGCAG 1 cut(s) 445
PstNI CAGNNNCTG 3 cut(s) 251, 254, 449
PsuI RGATCY 1 cut(s) 698
PvuII CAGCTG 2 cut(s) 251, 440
RsaI GTAC 2 cut(s) 563, 576
RsaNI GTAC 2 cut(s) 562, 575
SacI GAGCTC 1 cut(s) 476
SaqAI TTAA 4 cut(s) 153, 188, 635, 723
SatI GCNGC 5 cut(s) 249, 252, 336, 441, 462
Sau3AI GATC 4 cut(s) 522, 628, 698, 705
Sau96I GGNCC 2 cut(s) 85, 677
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 3 cut(s) 378, 516, 684
SduI GDGCHC 3 cut(s) 186, 328, 476
SexAI ACCWGGT 1 cut(s) 514
SfcI CTRYAG 1 cut(s) 441
SfuI TTCGAA 1 cut(s) 162
SinI GGWCC 1 cut(s) 677
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
Sse9I AATT 2 cut(s) 52, 306
SsiI CCGC 2 cut(s) 702, 716
SspMI CTAG 3 cut(s) 24, 59, 650
SstI GAGCTC 1 cut(s) 476
StyD4I CCNGG 3 cut(s) 376, 514, 682
StyI CCWWGG 1 cut(s) 58
TaaI ACNGT 3 cut(s) 106, 323, 566
TaiI ACGT 1 cut(s) 231
TaqI TCGA 3 cut(s) 162, 231, 646
TasI AATT 2 cut(s) 52, 306
TatI WGTACW 2 cut(s) 561, 574
TfiI GAWTC 3 cut(s) 290, 582, 731
Tru1I TTAA 4 cut(s) 153, 188, 635, 723
Tru9I TTAA 4 cut(s) 153, 188, 635, 723
TscAI CASTG 1 cut(s) 592
TseI GCWGC 5 cut(s) 248, 251, 335, 440, 461
TspDTI ATGAA 2 cut(s) 276, 394
TspRI CASTG 1 cut(s) 592
VpaK11BI GGWCC 1 cut(s) 677
XagI CCTNNNNNAGG 1 cut(s) 725
XceI RCATGY 1 cut(s) 562
XmaJI CCTAGG 1 cut(s) 58
XspI CTAG 3 cut(s) 24, 59, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.