AT4G25270

Pentatricopeptide repeat domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015204
Physical Location & Seq
Reverse (-)
12936931 .. 12938910
1980 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25270.1

Sequence Viewer

Length: 1584 bp
ATGGTGAGTATCGTTGTACACAAACCAAGCTTCTCTTATCCATCTGTATCGTCTTCTTCAATGAAAAAGAAACCCAGACATCACCAACAGTTGAAGCAACACAGACAAAACCAATACAACAACAATGGATTCACTTCCCTCTCTTTCACCAAACCATCCCCCACTCCTCTCTTGATCGAAAAACAATCCATTCACCGGACCCAACTCGAAGCTTTAGACTCTGTCATTACAGACCTCGAAACCTCCGCTCAAAAAGGCATCTCATTAACCGAACCAGAGATCTTCGCTTCTCTTCTCGAGACCTGCTACAGCTTAAGAGCTATTGACCATGGCGTTAGAGTTCATCACCTTATACCTCCCTATCTCTTGCGGAACAACCTGGGGATTTCATCGAAGCTTGTTAGACTCTACGCCTCTTGTGGCTACGCGGAAGTTGCACACGAAGTGTTCGATCGAATGTCTAAGAGAGACTCATCGCCTTTTGCTTGGAACTCTCTTATCTCAGGCTACGCTGAATTGGGTCAGTACGAAGACGCCATGGCTCTTTACTTTCAGATGGCTGAAGACGGCGTGAAACCAGACCGGTTCACATTTCCACGTGTTTTAAAGGCTTGTGGAGGAATTGGGTCTGTTCAAATTGGAGAAGCGATTCACCGTGATTTGGTTAAGGAAGGTTTTGGATACGATGTCTATGTGCTCAATGCTCTTGTTGTTATGTATGCGAAATGCGGCGACATTGTTAAAGCGAGGAATGTTTTTGACATGATTCCTCACAAAGACTATGTTTCTTGGAACTCCATGTTGACTGGTTACCTTCATCATGGCTTGTTACACGAGGCATTGGACATTTTTCGTCTAATGGTTCAGAATGGGATTGAGCCTGATAAGGTTGCTATCTCCAGTGTTCTAGCTCGTGTTTTGTCTTTCAAGCATGGACGTCAGCTACATGGATGGGTAATTAGGCGAGGCATGGAGTGGGAGTTATCGGTTGCGAATGCTTTGATTGTTCTATACTCCAAAAGGGGACAGTTGGGTCAAGCCTGCTTCATATTTGATCAGATGCTCGAGAGAGATACCGTATCTTGGAATGCTATTATCTCTGCTCATTCTAAAAATTCTAATGGTTTAAAGTATTTTGAGCAGATGCACCGTGCTAATGCTAAACCAGATGGTATCACGTTTGTCTCGGTTTTATCTCTCTGTGCTAATACAGGAATGGTCGAAGATGGAGAGAGGTTGTTCTCTTTGATGTCAAAGGAATACGGGATAGACCCGAAGATGGAACACTATGCGTGTATGGTGAATCTCTACGGAAGAGCAGGGATGATGGAAGAGGCTTACTCAATGATTGTTCAAGAGATGGGGCTTGAGGCTGGTCCAACTGTTTGGGGAGCTCTGTTATACGCTTGTTACCTCCATGGAAATACAGATATAGGAGAGGTTGCTGCTCAACGTCTTTTCGAATTAGAACCTGATAACGAACACAATTTCGAGCTGTTGATAAGAATTTATAGCAAAGCAAAAAGAGCAGAGGATGTTGAGAGAGTAAGGCAGATGATGGTGGATAGAGGATTGGAGACATAA

Protein Analysis

527

Amino Acids

59.53

Weight (kDa)

6.6

Isoelectric Point (pI)

35.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 161 - 205 9.2e-10 PPR repeat family
PPR PF01535 162 - 191 3e-07 PPR repeat
PPR_2 PF13041 260 - 302 1e-09 PPR repeat family
PPR PF01535 262 - 292 2.5e-08 PPR repeat
PPR_long PF17177 348 - 449 2.7e-06 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 473 - 525 4.2e-11 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25270
fragaria_vesca FvH4_7g27870
malus_domestica MD00G1104900.v1.1
prunus_persica Prupe.2G280000_v2.0.a1
pyrus_communis pycom01g19550
rosa_chinensis RchiOBHm_Chr1g0374811
rosa_laevigata RLG00000026713
rosa_multiflora Rmu_co8506051.1_g000002 Rmu_sc0004947.1_g000023
rosa_roxburghii Rroxscaffold_4G00283250
rosa_rugosa Rorug01G0385000
rosa_samantha Rh1AG396500 Rh1BG360600 Rh1CG374000 Rh1DG391400
rosa_wichuraiana Rw1G035150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1032
AatII GACGTC 1 cut(s) 940
Acc36I ACCTGC 1 cut(s) 311
AccBSI CCGCTC 1 cut(s) 248
AccII CGCG 1 cut(s) 428
AciI CCGC 4 cut(s) 246, 370, 428, 729
AcsI RAATTY 2 cut(s) 1114, 1506
AcuI CTGAAG 1 cut(s) 582
AcvI CACGTG 1 cut(s) 599
AcyI GRCGYC 2 cut(s) 534, 937
AdeI CACNNNGTG 1 cut(s) 445
AfaI GTAC 2 cut(s) 18, 527
AfiI CCNNNNNNNGG 4 cut(s) 195, 661, 1083, 1279
AflII CTTAAG 1 cut(s) 313
AflIII ACRYGT 1 cut(s) 598
AgeI ACCGGT 1 cut(s) 582
AgsI TTSAA 5 cut(s) 60, 94, 635, 928, 1355
AjnI CCWGG 1 cut(s) 378
AluBI AGCT 9 cut(s) 30, 212, 312, 320, 397, 911, 943, 1394, 1495
AluI AGCT 9 cut(s) 30, 212, 312, 320, 397, 911, 943, 1394, 1495
Alw21I GWGCWC 2 cut(s) 699, 1396
Alw26I GTCTC 4 cut(s) 293, 462, 1189, 1571
Ama87I CYCGRG 2 cut(s) 296, 1064
ApeKI GCWGC 1 cut(s) 1445
ApoI RAATTY 2 cut(s) 1114, 1506
AsiGI ACCGGT 1 cut(s) 582
Asp700I GAANNNNTTC 1 cut(s) 648
AspS9I GGNCC 2 cut(s) 198, 1376
AsuHPI GGTGA 7 cut(s) 16, 74, 139, 185, 338, 644, 1312
AsuII TTCGAA 1 cut(s) 1461
AvaI CYCGRG 2 cut(s) 296, 1064
AvaII GGWCC 2 cut(s) 198, 1376
BanII GRGCYC 1 cut(s) 1396
BauI CACGAG 2 cut(s) 833, 912
BbrPI CACGTG 1 cut(s) 599
BbsI GAAGAC 3 cut(s) 45, 537, 570
Bbv12I GWGCWC 2 cut(s) 699, 1396
BbvI GCAGC 1 cut(s) 1432
BceAI ACGGC 1 cut(s) 583
BciT130I CCWGG 1 cut(s) 380
BciVI GTATCC 1 cut(s) 674
BclI TGATCA 1 cut(s) 1054
BcoDI GTCTC 4 cut(s) 293, 462, 1189, 1571
BfaI CTAG 1 cut(s) 908
BfmI CTRYAG 1 cut(s) 307
BfrI CTTAAG 1 cut(s) 313
BfuAI ACCTGC 1 cut(s) 311
BfuI GTATCC 1 cut(s) 674
BglII AGATCT 1 cut(s) 279
BisI GCNGC 2 cut(s) 730, 1446
BlsI GCNGC 2 cut(s) 731, 1447
Bme1390I CCNGG 1 cut(s) 380
Bme18I GGWCC 2 cut(s) 198, 1376
BmeT110I CYCGRG 2 cut(s) 296, 1064
BmgT120I GGNCC 2 cut(s) 198, 1376
BmiI GGNNCC 1 cut(s) 200
BmrFI CCNGG 1 cut(s) 380
BmsI GCATC 3 cut(s) 267, 1050, 1134
BpiI GAAGAC 3 cut(s) 45, 537, 570
BplI GAGNNNNNCTC 2 cut(s) 1325, 1357
BpmI CTGGAG 1 cut(s) 883
Bpu14I TTCGAA 1 cut(s) 1461
BpuEI CTTGAG 1 cut(s) 1388
BsaAI YACGTR 1 cut(s) 599
BsaHI GRCGYC 2 cut(s) 534, 937
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 4 cut(s) 328, 379, 537, 1417
BsaWI WCCGGW 2 cut(s) 195, 582
BsaXI ACNNNNNCTCC 2 cut(s) 965, 995
Bsc4I CCNNNNNNNGG 4 cut(s) 195, 661, 1083, 1279
Bse118I RCCGGY 1 cut(s) 582
Bse1I ACTGG 2 cut(s) 811, 900
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 4 cut(s) 328, 379, 537, 1417
BseGI GGATG 4 cut(s) 155, 956, 1329, 1540
BseLI CCNNNNNNNGG 4 cut(s) 195, 661, 1083, 1279
BseMII CTCAG 1 cut(s) 516
BseNI ACTGG 2 cut(s) 811, 900
BseRI GAGGAG 1 cut(s) 156
BseXI GCAGC 1 cut(s) 1432
Bsh1236I CGCG 1 cut(s) 428
Bsh1285I CGRYCG 1 cut(s) 454
BshTI ACCGGT 1 cut(s) 582
BsiEI CGRYCG 1 cut(s) 454
BsiHKAI GWGCWC 2 cut(s) 699, 1396
BsiHKCI CYCGRG 2 cut(s) 296, 1064
BsiSI CCGG 2 cut(s) 196, 583
BslFI GGGAC 1 cut(s) 1038
BslI CCNNNNNNNGG 4 cut(s) 195, 661, 1083, 1279
BsmAI GTCTC 4 cut(s) 293, 462, 1189, 1571
BsmFI GGGAC 1 cut(s) 1038
BsmI GAATGC 2 cut(s) 1000, 1093
Bso31I GGTCTC 1 cut(s) 293
BsoBI CYCGRG 2 cut(s) 296, 1064
Bsp119I TTCGAA 1 cut(s) 1461
Bsp1286I GDGCHC 2 cut(s) 699, 1396
Bsp1407I TGTACA 1 cut(s) 16
Bsp143I GATC 4 cut(s) 174, 279, 451, 1054
Bsp19I CCATGG 3 cut(s) 328, 537, 1417
BspACI CCGC 4 cut(s) 246, 370, 428, 729
BspCNI CTCAG 1 cut(s) 515
BspFNI CGCG 1 cut(s) 428
BspLI GGNNCC 1 cut(s) 200
BspMI ACCTGC 1 cut(s) 311
BspQI GCTCTTC 1 cut(s) 1309
BspT104I TTCGAA 1 cut(s) 1461
BspTI CTTAAG 1 cut(s) 313
BspTNI GGTCTC 1 cut(s) 293
BsrBI CCGCTC 1 cut(s) 248
BsrFI RCCGGY 1 cut(s) 582
BsrGI TGTACA 1 cut(s) 16
BsrI ACTGG 2 cut(s) 811, 900
BssAI RCCGGY 1 cut(s) 582
BssECI CCNNGG 4 cut(s) 328, 379, 537, 1417
BssMI GATC 4 cut(s) 174, 279, 451, 1054
BssNI GRCGYC 2 cut(s) 534, 937
BssSI CACGAG 2 cut(s) 833, 912
BssT1I CCWWGG 3 cut(s) 328, 537, 1417
Bst2BI CACGAG 2 cut(s) 833, 912
Bst2UI CCWGG 1 cut(s) 380
Bst4CI ACNGT 6 cut(s) 90, 656, 1029, 1078, 1151, 1384
Bst6I CTCTTC 3 cut(s) 297, 1309, 1326
BstACI GRCGYC 2 cut(s) 534, 937
BstAFI CTTAAG 1 cut(s) 313
BstAUI TGTACA 1 cut(s) 16
BstBAI YACGTR 1 cut(s) 599
BstBI TTCGAA 1 cut(s) 1461
BstC8I GCNNGC 1 cut(s) 1042
BstDEI CTNAG 2 cut(s) 462, 502
BstDSI CCRYGG 3 cut(s) 328, 537, 1417
BstEII GGTNACC 1 cut(s) 809
BstF5I GGATG 4 cut(s) 155, 956, 1329, 1540
BstFNI CGCG 1 cut(s) 428
BstKTI GATC 4 cut(s) 177, 282, 454, 1057
BstMAI GTCTC 4 cut(s) 293, 462, 1189, 1571
BstMBI GATC 4 cut(s) 174, 279, 451, 1054
BstMCI CGRYCG 1 cut(s) 454
BstMWI GCNNNNNNNGC 2 cut(s) 434, 1526
BstNI CCWGG 1 cut(s) 380
BstPI GGTNACC 1 cut(s) 809
BstSCI CCNGG 1 cut(s) 378
BstSFI CTRYAG 1 cut(s) 307
BstUI CGCG 1 cut(s) 428
BstV1I GCAGC 1 cut(s) 1432
BstV2I GAAGAC 3 cut(s) 45, 537, 570
BstX2I RGATCY 1 cut(s) 279
BstXI CCANNNNNNTGG 1 cut(s) 1386
BstYI RGATCY 1 cut(s) 279
BsuI GTATCC 1 cut(s) 674
BtgI CCRYGG 3 cut(s) 328, 537, 1417
BtgZI GCGATG 1 cut(s) 459
BtsCI GGATG 4 cut(s) 155, 956, 1329, 1540
BtsIMutI CAGTG 1 cut(s) 907
BveI ACCTGC 1 cut(s) 311
Cac8I GCNNGC 1 cut(s) 1042
Cfr10I RCCGGY 1 cut(s) 582
Cfr13I GGNCC 2 cut(s) 198, 1376
CseI GACGC 1 cut(s) 542
Csp6I GTAC 2 cut(s) 17, 526
CspAI ACCGGT 1 cut(s) 582
CviAII CATG 9 cut(s) 329, 538, 763, 799, 821, 932, 947, 970, 1418
CviQI GTAC 2 cut(s) 17, 526
DdeI CTNAG 2 cut(s) 462, 502
DpnI GATC 4 cut(s) 176, 281, 453, 1056
DpnII GATC 4 cut(s) 174, 279, 451, 1054
DraI TTTAAA 2 cut(s) 606, 1128
DraIII CACNNNGTG 1 cut(s) 445
DrdI GACNNNNNNGTC 1 cut(s) 1032
DseDI GACNNNNNNGTC 1 cut(s) 1032
Eam1104I CTCTTC 3 cut(s) 297, 1309, 1326
EarI CTCTTC 3 cut(s) 297, 1309, 1326
Ecl136II GAGCTC 1 cut(s) 1394
Eco130I CCWWGG 3 cut(s) 328, 537, 1417
Eco24I GRGCYC 1 cut(s) 1396
Eco31I GGTCTC 1 cut(s) 293
Eco47I GGWCC 2 cut(s) 198, 1376
Eco53kI GAGCTC 1 cut(s) 1394
Eco57I CTGAAG 1 cut(s) 582
Eco72I CACGTG 1 cut(s) 599
Eco88I CYCGRG 2 cut(s) 296, 1064
Eco91I GGTNACC 1 cut(s) 809
EcoICRI GAGCTC 1 cut(s) 1394
EcoO65I GGTNACC 1 cut(s) 809
EcoRII CCWGG 1 cut(s) 378
EcoT14I CCWWGG 3 cut(s) 328, 537, 1417
EcoT38I GRGCYC 1 cut(s) 1396
ErhI CCWWGG 3 cut(s) 328, 537, 1417
FaeI CATG 9 cut(s) 332, 541, 766, 802, 824, 935, 950, 973, 1421
FalI AAGNNNNNCTT 2 cut(s) 19, 51
FaqI GGGAC 1 cut(s) 1038
FatI CATG 9 cut(s) 328, 537, 762, 798, 820, 931, 946, 969, 1417
FbaI TGATCA 1 cut(s) 1054
Fnu4HI GCNGC 2 cut(s) 730, 1446
FokI GGATG 4 cut(s) 142, 963, 1336, 1547
FriOI GRGCYC 1 cut(s) 1396
Fsp4HI GCNGC 2 cut(s) 730, 1446
FspBI CTAG 1 cut(s) 908
GluI GCNGC 2 cut(s) 730, 1446
GsuI CTGGAG 1 cut(s) 883
HapII CCGG 2 cut(s) 196, 583
HgaI GACGC 1 cut(s) 542
Hin1I GRCGYC 2 cut(s) 534, 937
Hin1II CATG 9 cut(s) 332, 541, 766, 802, 824, 935, 950, 973, 1421
HincII GTYRAC 1 cut(s) 804
HindII GTYRAC 1 cut(s) 804
HindIII AAGCTT 3 cut(s) 28, 210, 395
HinfI GANTC 7 cut(s) 129, 218, 405, 470, 649, 766, 1303
HpaII CCGG 2 cut(s) 196, 583
HphI GGTGA 7 cut(s) 16, 74, 139, 185, 338, 644, 1312
Hpy166II GTNNAC 3 cut(s) 19, 588, 804
Hpy188I TCNGA 3 cut(s) 555, 867, 1059
Hpy188III TCNNGA 5 cut(s) 172, 296, 298, 1066, 1355
Hpy8I GTNNAC 3 cut(s) 19, 588, 804
HpyAV CCTTC 2 cut(s) 665, 824
HpyCH4III ACNGT 6 cut(s) 90, 656, 1029, 1078, 1151, 1384
HpyCH4IV ACGT 4 cut(s) 598, 937, 1178, 1453
HpyCH4V TGCA 2 cut(s) 437, 1147
HpyF10VI GCNNNNNNNGC 2 cut(s) 434, 1526
HpyF3I CTNAG 2 cut(s) 462, 502
HpySE526I ACGT 4 cut(s) 598, 937, 1178, 1453
Hsp92I GRCGYC 2 cut(s) 534, 937
Hsp92II CATG 9 cut(s) 332, 541, 766, 802, 824, 935, 950, 973, 1421
Ksp22I TGATCA 1 cut(s) 1054
Kzo9I GATC 4 cut(s) 174, 279, 451, 1054
LguI GCTCTTC 1 cut(s) 1309
LmnI GCTCC 1 cut(s) 1391
Lsp1109I GCAGC 1 cut(s) 1432
LweI GCATC 3 cut(s) 267, 1050, 1134
MaeI CTAG 1 cut(s) 908
MaeII ACGT 4 cut(s) 598, 937, 1178, 1453
MaeIII GTNAC 3 cut(s) 809, 828, 1409
MalI GATC 4 cut(s) 176, 281, 453, 1056
MbiI CCGCTC 1 cut(s) 248
MboI GATC 4 cut(s) 174, 279, 451, 1054
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 2 cut(s) 699, 1396
MluCI AATT 8 cut(s) 515, 621, 636, 957, 1114, 1463, 1486, 1506
MlyI GAGTC 3 cut(s) 212, 399, 464
MmeI TCCRAC 1 cut(s) 1403
MroXI GAANNNNTTC 1 cut(s) 648
MseI TTAA 6 cut(s) 266, 314, 605, 666, 741, 1127
MspCI CTTAAG 1 cut(s) 313
MspI CCGG 2 cut(s) 196, 583
MspR9I CCNGG 1 cut(s) 380
Mva1269I GAATGC 2 cut(s) 1000, 1093
MvaI CCWGG 1 cut(s) 380
MvnI CGCG 1 cut(s) 428
MwoI GCNNNNNNNGC 2 cut(s) 434, 1526
NcoI CCATGG 3 cut(s) 328, 537, 1417
NdeII GATC 4 cut(s) 174, 279, 451, 1054
NlaIII CATG 9 cut(s) 332, 541, 766, 802, 824, 935, 950, 973, 1421
NlaIV GGNNCC 1 cut(s) 200
NspV TTCGAA 1 cut(s) 1461
PaeR7I CTCGAG 2 cut(s) 296, 1064
PciSI GCTCTTC 1 cut(s) 1309
PcsI WCGNNNNNNNCGW 1 cut(s) 447
PctI GAATGC 2 cut(s) 1000, 1093
PdmI GAANNNNTTC 1 cut(s) 648
PfeI GAWTC 4 cut(s) 129, 649, 766, 1303
PflFI GACNNNGTC 1 cut(s) 221
PinAI ACCGGT 1 cut(s) 582
PkrI GCNGC 2 cut(s) 731, 1447
Ple19I CGATCG 1 cut(s) 454
PleI GAGTC 3 cut(s) 212, 399, 464
PmaCI CACGTG 1 cut(s) 599
PmlI CACGTG 1 cut(s) 599
PpsI GAGTC 3 cut(s) 212, 399, 464
Ppu21I YACGTR 1 cut(s) 599
Psp124BI GAGCTC 1 cut(s) 1396
Psp6I CCWGG 1 cut(s) 378
PspCI CACGTG 1 cut(s) 599
PspEI GGTNACC 1 cut(s) 809
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 1 cut(s) 200
PspPI GGNCC 2 cut(s) 198, 1376
PsuI RGATCY 1 cut(s) 279
PsyI GACNNNGTC 1 cut(s) 221
PvuI CGATCG 1 cut(s) 454
RsaI GTAC 2 cut(s) 18, 527
RsaNI GTAC 2 cut(s) 17, 526
SacI GAGCTC 1 cut(s) 1396
SapI GCTCTTC 1 cut(s) 1309
SaqAI TTAA 6 cut(s) 266, 314, 605, 666, 741, 1127
SatI GCNGC 2 cut(s) 730, 1446
Sau3AI GATC 4 cut(s) 174, 279, 451, 1054
Sau96I GGNCC 2 cut(s) 198, 1376
SchI GAGTC 3 cut(s) 212, 399, 464
ScrFI CCNGG 1 cut(s) 380
SduI GDGCHC 2 cut(s) 699, 1396
SfaNI GCATC 3 cut(s) 267, 1050, 1134
SfcI CTRYAG 1 cut(s) 307
Sfr274I CTCGAG 2 cut(s) 296, 1064
SfuI TTCGAA 1 cut(s) 1461
SinI GGWCC 2 cut(s) 198, 1376
SlaI CTCGAG 2 cut(s) 296, 1064
SmlI CTYRAG 4 cut(s) 296, 313, 1064, 1367
SmoI CTYRAG 4 cut(s) 296, 313, 1064, 1367
Sse9I AATT 8 cut(s) 515, 621, 636, 957, 1114, 1463, 1486, 1506
SsiI CCGC 4 cut(s) 246, 370, 428, 729
SspMI CTAG 1 cut(s) 908
SstI GAGCTC 1 cut(s) 1396
StyD4I CCNGG 1 cut(s) 378
StyI CCWWGG 3 cut(s) 328, 537, 1417
TaaI ACNGT 6 cut(s) 90, 656, 1029, 1078, 1151, 1384
TaiI ACGT 4 cut(s) 601, 940, 1181, 1456
TasI AATT 8 cut(s) 515, 621, 636, 957, 1114, 1463, 1486, 1506
TatI WGTACW 1 cut(s) 16
TauI GCSGC 1 cut(s) 732
TfiI GAWTC 4 cut(s) 129, 649, 766, 1303
Tru1I TTAA 6 cut(s) 266, 314, 605, 666, 741, 1127
Tru9I TTAA 6 cut(s) 266, 314, 605, 666, 741, 1127
TscAI CASTG 1 cut(s) 907
TseI GCWGC 1 cut(s) 1445
TspDTI ATGAA 5 cut(s) 77, 332, 378, 806, 1036
TspGWI ACGGA 1 cut(s) 1326
TspRI CASTG 1 cut(s) 907
Tth111I GACNNNGTC 1 cut(s) 221
Vha464I CTTAAG 1 cut(s) 313
VpaK11BI GGWCC 2 cut(s) 198, 1376
XapI RAATTY 2 cut(s) 1114, 1506
XhoI CTCGAG 2 cut(s) 296, 1064
XmnI GAANNNNTTC 1 cut(s) 648
XspI CTAG 1 cut(s) 908
ZraI GACGTC 1 cut(s) 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.