Rh1CG374000

Pentatricopeptide repeat-containing protein At4g25270

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0015204
Physical Location & Seq
Reverse (-)
64323402 .. 64324964
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG374000.1

Sequence Viewer

Length: 1563 bp
ATGGTGACCACTTTACAACCCTTAAGTCTACACACAACAAAACTCACTATCCACTGCTCCTCTAATAGCAAGAGAAGCAAGAAGCCCAAGCAGCTTCACCAAAAGCAGAGCACCAAGCTCCTCTCTTTCTCAAACTCATCCCCAGCCCCTCTCATTATCTACCAAAAACCCCAAACCCAGACCAAGCTCCAAGCCCTCGAGGCCATCATCAAGGACCTTGAAGCCTCTAGCGAAAACGGCATAGACGTCGACACTGAAACCTTTGCTTCCCTTTTAGAAACATGTTACAAATTAGACGCGATGGACTACTGTCTCCGGGTCCACAGACTAATACCCAGAAACCTTTTACGTAGGAATGTAGGCCTCTCATCAAAGCTTCTTCGTCTTTACGCGTCGTGTGGGTACATTGAGGAAGCACACCAGGTGTTCGATCAAATGCCCAAGAGAGACGTTTCGGCTTTTGCGTGGAATTCACTTATATCCGGGTATGCTGAGTTGGGTTTGTATGAAGATGCTATGGCGCTGTACTTTCAAATGGAGGAGGAAGGGGTTGAACCGGACCGGTTTACGTTCCCTCGAGTTTTGAAAGCTTGTGGGGGAATTGGGTTTGTTCAGATTGGTGAGGCTGTGCATAGGCATGCGGTGCGTTTGGGTTTTGTAGGTGATAGGTTTGTGCTTAATGCACTAGTGGACATGTATGCTAAATGTGGTGACATTGTGAAGGCTAGGAAGGTGTTTGATAAGATTGGTAGCCGGGACAAGGTTTCTTGGAACACAATGCTCACTGCTTATATGCGCCACGGCCTTTTGTTGCAGGCATTGGACATTTTTCACCAAATGGTTAGAGAAGGGTACCAGCCGGACTCCGTTACTATATCCACAATTCTCACCGCAGTACCATCACTGGAGCTTGTTGTTCAAATTCATGGGTGGACGATTCGGCAAGGAGTTGAGTGGAACCTGTCTATTGCTAATTCCTTGATTGCTGCATATTCCAATCATGGAAAGTTGAAGCGCGCTCGTTGGCTGTTTTGTCAAATGCCTGAGAGGGACGTGGTGACATGGAATACTATAATTTCAGCTCATTCTAAAAGCCGAGAAGCTTTGGTGTACTTTGAGCAGATGGAGAGTGCCGGTGCCTTGCCGGATGCTATCACATTCGTGTCAATGCTCTCGGTTTGTGCGCATTTGGGTTTGGTGGAGGATGGGGAGAGATTGTTTTCTACCATGAAAAACAGATACAGAATTAGCCCAATTATGGAACATTATGCTTGTATGGTGAATCTTTATGGAAGGGCAGGTCTAATCAAAGAAGCTTATGGTATTATAATGGAGGGAATGGAGTTTGAGGCTGGTCCAACTGTGTGGGGTGCATTGCTATATGCTTGCTACCTTTATGGGAATGCAGAGATTGGAGAGGTTGCAGCTGAAAGACTCTTCGACTTGGAGCCTGATAATGAATATAATTTTGAGCTTCTGATGAAGATTTACGGCAATGTGGGTAGACTGGACAATGTAGAGAGAGTAAGAATGATGATGGTGGATAGAGGACTGGACTCGTAG

Protein Analysis

520

Amino Acids

58.79

Weight (kDa)

6.55

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 153 - 198 6e-10 PPR repeat family
PPR PF01535 155 - 184 7.9e-08 PPR repeat
PPR_2 PF13041 253 - 298 2e-12 PPR repeat family
PPR PF01535 255 - 284 1.3e-07 PPR repeat
PPR_2 PF13041 350 - 396 3.4e-08 PPR repeat family
E_motif PF20431 466 - 517 4e-09 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25270
fragaria_vesca FvH4_7g27870
malus_domestica MD00G1104900.v1.1
prunus_persica Prupe.2G280000_v2.0.a1
pyrus_communis pycom01g19550
rosa_chinensis RchiOBHm_Chr1g0374811
rosa_laevigata RLG00000026713
rosa_multiflora Rmu_co8506051.1_g000002 Rmu_sc0004947.1_g000023
rosa_roxburghii Rroxscaffold_4G00283250
rosa_rugosa Rorug01G0385000
rosa_samantha Rh1AG396500 Rh1BG360600 Rh1CG374000 Rh1DG391400
rosa_wichuraiana Rw1G035150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1328
AatII GACGTC 1 cut(s) 249
AbsI CCTCGAGG 1 cut(s) 197
Acc16I TGCGCA 1 cut(s) 1185
Acc36I ACCTGC 1 cut(s) 1289
Acc65I GGTACC 1 cut(s) 852
AccB1I GGYRCC 2 cut(s) 852, 1136
AccI GTMKAC 3 cut(s) 28, 249, 1504
AccII CGCG 3 cut(s) 299, 392, 1017
AciI CCGC 2 cut(s) 641, 891
AcsI RAATTY 2 cut(s) 469, 921
AcyI GRCGYC 1 cut(s) 246
AdeI CACNNNGTG 1 cut(s) 424
AfaI GTAC 5 cut(s) 404, 527, 854, 897, 1112
AfiI CCNNNNNNNGG 2 cut(s) 760, 1258
AflII CTTAAG 1 cut(s) 22
AflIII ACRYGT 3 cut(s) 281, 390, 693
AgeI ACCGGT 1 cut(s) 561
AgsI TTSAA 6 cut(s) 221, 533, 554, 586, 920, 1012
AhlI ACTAGT 1 cut(s) 685
AjiI CACGTC 1 cut(s) 1054
AjnI CCWGG 1 cut(s) 420
AleI CACNNNNGTG 1 cut(s) 1160
Alw21I GWGCWC 1 cut(s) 113
Alw26I GTCTC 2 cut(s) 317, 441
Ama87I CYCGRG 2 cut(s) 197, 576
AoxI GGCC 3 cut(s) 201, 361, 802
ApeKI GCWGC 3 cut(s) 91, 986, 1424
ApoI RAATTY 2 cut(s) 469, 921
AsiGI ACCGGT 1 cut(s) 561
Asp718I GGTACC 1 cut(s) 852
AspLEI GCGC 5 cut(s) 523, 798, 1017, 1019, 1186
AspS9I GGNCC 4 cut(s) 214, 319, 559, 1355
AsuC2I CCSGG 3 cut(s) 317, 484, 755
AsuHPI GGTGA 9 cut(s) 16, 89, 632, 674, 722, 824, 880, 1069, 1291
AvaI CYCGRG 2 cut(s) 197, 576
AvaII GGWCC 4 cut(s) 214, 319, 559, 1355
BaeI ACNNNNGTAYC 2 cut(s) 879, 912
BanI GGYRCC 2 cut(s) 852, 1136
Bbv12I GWGCWC 1 cut(s) 113
BbvI GCAGC 3 cut(s) 103, 973, 1436
BccI CCATC 6 cut(s) 212, 295, 907, 1117, 1199, 1531
BceAI ACGGC 3 cut(s) 253, 817, 1507
BcgI CGANNNNNNTGC 2 cut(s) 229, 263
BciT130I CCWGG 1 cut(s) 422
BcnI CCSGG 3 cut(s) 317, 484, 755
BcoDI GTCTC 2 cut(s) 317, 441
BcuI ACTAGT 1 cut(s) 685
BfaI CTAG 3 cut(s) 228, 686, 726
BfoI RGCGCY 1 cut(s) 524
BfrI CTTAAG 1 cut(s) 22
BfuAI ACCTGC 1 cut(s) 1289
BglI GCCNNNNNGGC 1 cut(s) 200
BisI GCNGC 3 cut(s) 92, 987, 1425
BlsI GCNGC 3 cut(s) 93, 988, 1426
Bme1390I CCNGG 4 cut(s) 317, 422, 484, 755
Bme18I GGWCC 4 cut(s) 214, 319, 559, 1355
BmeT110I CYCGRG 2 cut(s) 197, 576
BmgBI CACGTC 1 cut(s) 1054
BmgT120I GGNCC 4 cut(s) 214, 319, 559, 1355
BmiI GGNNCC 5 cut(s) 320, 854, 959, 1138, 1449
BmrFI CCNGG 4 cut(s) 317, 422, 484, 755
BmsI GCATC 2 cut(s) 502, 1138
BoxI GACNNNNGTC 1 cut(s) 309
BpmI CTGGAG 1 cut(s) 926
BpuMI CCSGG 3 cut(s) 317, 484, 755
BsaAI YACGTR 1 cut(s) 350
BsaHI GRCGYC 1 cut(s) 246
BsaJI CCNNGG 1 cut(s) 799
BsaWI WCCGGW 2 cut(s) 556, 561
BsaXI ACNNNNNCTCC 2 cut(s) 1202, 1232
Bsc4I CCNNNNNNNGG 2 cut(s) 760, 1258
Bse118I RCCGGY 2 cut(s) 561, 1133
Bse1I ACTGG 3 cut(s) 909, 1512, 1557
Bse3DI GCAATG 2 cut(s) 1373, 1501
BseBI CCWGG 1 cut(s) 422
BseDI CCNNGG 1 cut(s) 799
BseGI GGATG 3 cut(s) 137, 1153, 1210
BseLI CCNNNNNNNGG 2 cut(s) 760, 1258
BseMI GCAATG 2 cut(s) 1373, 1501
BseMII CTCAG 2 cut(s) 483, 1035
BseNI ACTGG 3 cut(s) 909, 1512, 1557
BsePI GCGCGC 1 cut(s) 1015
BseRI GAGGAG 3 cut(s) 49, 110, 554
BseXI GCAGC 3 cut(s) 103, 973, 1436
BseYI CCCAGC 1 cut(s) 142
Bsh1236I CGCG 3 cut(s) 299, 392, 1017
BshFI GGCC 3 cut(s) 203, 363, 804
BshNI GGYRCC 2 cut(s) 852, 1136
BshTI ACCGGT 1 cut(s) 561
BsiHKAI GWGCWC 1 cut(s) 113
BsiHKCI CYCGRG 2 cut(s) 197, 576
BsiSI CCGG 8 cut(s) 316, 483, 557, 562, 754, 860, 1134, 1145
BslFI GGGAC 2 cut(s) 770, 1064
BslI CCNNNNNNNGG 2 cut(s) 760, 1258
BsmAI GTCTC 2 cut(s) 317, 441
BsmBI CGTCTC 1 cut(s) 441
BsmFI GGGAC 2 cut(s) 770, 1064
BsmI GAATGC 1 cut(s) 1408
BsnI GGCC 3 cut(s) 203, 363, 804
BsoBI CYCGRG 2 cut(s) 197, 576
Bsp1286I GDGCHC 1 cut(s) 113
Bsp143I GATC 1 cut(s) 430
BspACI CCGC 2 cut(s) 641, 891
BspANI GGCC 3 cut(s) 203, 363, 804
BspCNI CTCAG 2 cut(s) 484, 1036
BspFNI CGCG 3 cut(s) 299, 392, 1017
BspLI GGNNCC 5 cut(s) 320, 854, 959, 1138, 1449
BspMI ACCTGC 1 cut(s) 1289
BspT107I GGYRCC 2 cut(s) 852, 1136
BspTI CTTAAG 1 cut(s) 22
BsrDI GCAATG 2 cut(s) 1373, 1501
BsrFI RCCGGY 2 cut(s) 561, 1133
BsrI ACTGG 3 cut(s) 909, 1512, 1557
BssAI RCCGGY 2 cut(s) 561, 1133
BssECI CCNNGG 1 cut(s) 799
BssHII GCGCGC 1 cut(s) 1015
BssMI GATC 1 cut(s) 430
BssNI GRCGYC 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 422
Bst4CI ACNGT 2 cut(s) 311, 1363
Bst6I CTCTTC 1 cut(s) 1442
BstACI GRCGYC 1 cut(s) 246
BstAFI CTTAAG 1 cut(s) 22
BstAPI GCANNNNNTGC 1 cut(s) 643
BstBAI YACGTR 1 cut(s) 350
BstC8I GCNNGC 4 cut(s) 639, 816, 1017, 1387
BstDEI CTNAG 2 cut(s) 492, 1044
BstDSI CCRYGG 1 cut(s) 799
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 3 cut(s) 137, 1153, 1210
BstFNI CGCG 3 cut(s) 299, 392, 1017
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 5 cut(s) 523, 798, 1017, 1019, 1186
BstKTI GATC 1 cut(s) 433
BstMAI GTCTC 2 cut(s) 317, 441
BstMBI GATC 1 cut(s) 430
BstMWI GCNNNNNNNGC 5 cut(s) 75, 91, 200, 237, 643
BstNI CCWGG 1 cut(s) 422
BstNSI RCATGY 3 cut(s) 285, 641, 697
BstPAI GACNNNNGTC 1 cut(s) 309
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 4 cut(s) 315, 420, 482, 753
BstSNI TACGTA 1 cut(s) 350
BstUI CGCG 3 cut(s) 299, 392, 1017
BstV1I GCAGC 3 cut(s) 103, 973, 1436
BstXI CCANNNNNNTGG 1 cut(s) 1365
BsuRI GGCC 3 cut(s) 203, 363, 804
BtgI CCRYGG 1 cut(s) 799
BtgZI GCGATG 1 cut(s) 314
BtrI CACGTC 1 cut(s) 1054
BtsCI GGATG 3 cut(s) 137, 1153, 1210
BtsI GCAGTG 2 cut(s) 52, 783
BtsIMutI CAGTG 4 cut(s) 52, 252, 783, 902
BveI ACCTGC 1 cut(s) 1289
Cac8I GCNNGC 4 cut(s) 639, 816, 1017, 1387
CfoI GCGC 5 cut(s) 523, 798, 1017, 1019, 1186
Cfr10I RCCGGY 2 cut(s) 561, 1133
Cfr13I GGNCC 4 cut(s) 214, 319, 559, 1355
CpoI CGGWCCG 1 cut(s) 559
CseI GACGC 2 cut(s) 305, 381
CsiI ACCWGGT 1 cut(s) 420
Csp6I GTAC 5 cut(s) 403, 526, 853, 896, 1111
CspAI ACCGGT 1 cut(s) 561
CspI CGGWCCG 1 cut(s) 559
CviAII CATG 7 cut(s) 282, 638, 694, 926, 1001, 1062, 1228
CviQI GTAC 5 cut(s) 403, 526, 853, 896, 1111
DdeI CTNAG 2 cut(s) 492, 1044
DpnI GATC 1 cut(s) 432
DpnII GATC 1 cut(s) 430
DraIII CACNNNGTG 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 1442
EarI CTCTTC 1 cut(s) 1442
Eco105I TACGTA 1 cut(s) 350
Eco147I AGGCCT 1 cut(s) 363
Eco47I GGWCC 4 cut(s) 214, 319, 559, 1355
Eco88I CYCGRG 2 cut(s) 197, 576
Eco91I GGTNACC 1 cut(s) 4
EcoO109I RGGNCCY 1 cut(s) 214
EcoO65I GGTNACC 1 cut(s) 4
EcoRI GAATTC 1 cut(s) 469
EcoRII CCWGG 1 cut(s) 420
Esp3I CGTCTC 1 cut(s) 441
FaeI CATG 7 cut(s) 285, 641, 697, 929, 1004, 1065, 1231
FaqI GGGAC 2 cut(s) 770, 1064
FatI CATG 7 cut(s) 281, 637, 693, 925, 1000, 1061, 1227
FblI GTMKAC 3 cut(s) 28, 249, 1504
Fnu4HI GCNGC 3 cut(s) 92, 987, 1425
FokI GGATG 3 cut(s) 124, 1160, 1217
Fsp4HI GCNGC 3 cut(s) 92, 987, 1425
FspAI RTGCGCAY 1 cut(s) 1185
FspBI CTAG 3 cut(s) 228, 686, 726
FspI TGCGCA 1 cut(s) 1185
GlaI GCGC 5 cut(s) 522, 797, 1016, 1018, 1185
GluI GCNGC 3 cut(s) 92, 987, 1425
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 1 cut(s) 926
HaeII RGCGCY 1 cut(s) 524
HaeIII GGCC 3 cut(s) 203, 363, 804
HapII CCGG 8 cut(s) 316, 483, 557, 562, 754, 860, 1134, 1145
HgaI GACGC 2 cut(s) 305, 381
HhaI GCGC 5 cut(s) 523, 798, 1017, 1019, 1186
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 7 cut(s) 285, 641, 697, 929, 1004, 1065, 1231
Hin6I GCGC 5 cut(s) 521, 796, 1015, 1017, 1184
HinP1I GCGC 5 cut(s) 521, 796, 1015, 1017, 1184
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HindIII AAGCTT 4 cut(s) 374, 588, 1101, 1314
HinfI GANTC 5 cut(s) 863, 937, 1282, 1434, 1556
HpaII CCGG 8 cut(s) 316, 483, 557, 562, 754, 860, 1134, 1145
HphI GGTGA 9 cut(s) 16, 89, 632, 674, 722, 824, 880, 1069, 1291
Hpy166II GTNNAC 8 cut(s) 29, 250, 322, 567, 691, 933, 1111, 1505
Hpy188I TCNGA 2 cut(s) 615, 1479
Hpy8I GTNNAC 8 cut(s) 29, 250, 322, 567, 691, 933, 1111, 1505
Hpy99I CGWCG 2 cut(s) 251, 397
HpyAV CCTTC 5 cut(s) 539, 715, 724, 842, 1287
HpyCH4III ACNGT 2 cut(s) 311, 1363
HpyCH4IV ACGT 5 cut(s) 246, 349, 450, 569, 1053
HpyCH4V TGCA 7 cut(s) 631, 683, 814, 989, 1373, 1406, 1424
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 91, 200, 237, 643
HpyF3I CTNAG 2 cut(s) 492, 1044
HpySE526I ACGT 5 cut(s) 246, 349, 450, 569, 1053
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 7 cut(s) 285, 641, 697, 929, 1004, 1065, 1231
HspAI GCGC 5 cut(s) 521, 796, 1015, 1017, 1184
KpnI GGTACC 1 cut(s) 856
Kzo9I GATC 1 cut(s) 430
LmnI GCTCC 5 cut(s) 62, 123, 192, 907, 1447
Lsp1109I GCAGC 3 cut(s) 103, 973, 1436
LweI GCATC 2 cut(s) 502, 1138
MabI ACCWGGT 1 cut(s) 420
MaeI CTAG 3 cut(s) 228, 686, 726
MaeII ACGT 5 cut(s) 246, 349, 450, 569, 1053
MaeIII GTNAC 5 cut(s) 4, 284, 710, 868, 1057
MalI GATC 1 cut(s) 432
MboI GATC 1 cut(s) 430
MboII GAAGA 4 cut(s) 371, 521, 1429, 1495
MhlI GDGCHC 1 cut(s) 113
MluI ACGCGT 1 cut(s) 390
MlyI GAGTC 3 cut(s) 857, 1428, 1550
MmeI TCCRAC 1 cut(s) 1382
MseI TTAA 2 cut(s) 23, 678
MslI CAYNNNNRTG 2 cut(s) 636, 1160
MspA1I CMGCKG 1 cut(s) 1427
MspCI CTTAAG 1 cut(s) 22
MspI CCGG 8 cut(s) 316, 483, 557, 562, 754, 860, 1134, 1145
MspR9I CCNGG 4 cut(s) 317, 422, 484, 755
Mva1269I GAATGC 1 cut(s) 1408
MvaI CCWGG 1 cut(s) 422
MvnI CGCG 3 cut(s) 299, 392, 1017
MwoI GCNNNNNNNGC 5 cut(s) 75, 91, 200, 237, 643
NciI CCSGG 3 cut(s) 317, 484, 755
NdeII GATC 1 cut(s) 430
NlaIII CATG 7 cut(s) 285, 641, 697, 929, 1004, 1065, 1231
NlaIV GGNNCC 5 cut(s) 320, 854, 959, 1138, 1449
NmeAIII GCCGAG 1 cut(s) 1121
NmuCI GTSAC 3 cut(s) 4, 710, 1057
NsbI TGCGCA 1 cut(s) 1185
NspI RCATGY 3 cut(s) 285, 641, 697
OliI CACNNNNGTG 1 cut(s) 1160
PaeI GCATGC 1 cut(s) 641
PaeR7I CTCGAG 2 cut(s) 197, 576
PauI GCGCGC 1 cut(s) 1015
PceI AGGCCT 1 cut(s) 363
PciI ACATGT 2 cut(s) 281, 693
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 461
PctI GAATGC 1 cut(s) 1408
PfeI GAWTC 2 cut(s) 937, 1282
PinAI ACCGGT 1 cut(s) 561
PkrI GCNGC 3 cut(s) 93, 988, 1426
PleI GAGTC 3 cut(s) 857, 1428, 1550
PpsI GAGTC 3 cut(s) 857, 1428, 1550
Ppu21I YACGTR 1 cut(s) 350
PpuMI RGGWCCY 1 cut(s) 214
PscI ACATGT 2 cut(s) 281, 693
PshAI GACNNNNGTC 1 cut(s) 309
PsiI TTATAA 1 cut(s) 1328
Psp5II RGGWCCY 1 cut(s) 214
Psp6I CCWGG 1 cut(s) 420
PspEI GGTNACC 1 cut(s) 4
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 1 cut(s) 420
PspN4I GGNNCC 5 cut(s) 320, 854, 959, 1138, 1449
PspPI GGNCC 4 cut(s) 214, 319, 559, 1355
PspPPI RGGWCCY 1 cut(s) 214
PspXI VCTCGAGB 2 cut(s) 197, 576
PteI GCGCGC 1 cut(s) 1015
PvuII CAGCTG 1 cut(s) 1427
RsaI GTAC 5 cut(s) 404, 527, 854, 897, 1112
RsaNI GTAC 5 cut(s) 403, 526, 853, 896, 1111
RseI CAYNNNNRTG 2 cut(s) 636, 1160
Rsr2I CGGWCCG 1 cut(s) 559
RsrII CGGWCCG 1 cut(s) 559
SalI GTCGAC 1 cut(s) 248
SaqAI TTAA 2 cut(s) 23, 678
SatI GCNGC 3 cut(s) 92, 987, 1425
Sau3AI GATC 1 cut(s) 430
Sau96I GGNCC 4 cut(s) 214, 319, 559, 1355
SchI GAGTC 3 cut(s) 857, 1428, 1550
ScrFI CCNGG 4 cut(s) 317, 422, 484, 755
SduI GDGCHC 1 cut(s) 113
SexAI ACCWGGT 1 cut(s) 420
SfaNI GCATC 2 cut(s) 502, 1138
Sfr274I CTCGAG 2 cut(s) 197, 576
SinI GGWCC 4 cut(s) 214, 319, 559, 1355
SlaI CTCGAG 2 cut(s) 197, 576
SmiMI CAYNNNNRTG 2 cut(s) 636, 1160
SmlI CTYRAG 3 cut(s) 22, 197, 576
SmoI CTYRAG 3 cut(s) 22, 197, 576
SnaBI TACGTA 1 cut(s) 350
SpeI ACTAGT 1 cut(s) 685
SphI GCATGC 1 cut(s) 641
SseBI AGGCCT 1 cut(s) 363
SsiI CCGC 2 cut(s) 641, 891
SspMI CTAG 3 cut(s) 228, 686, 726
StuI AGGCCT 1 cut(s) 363
StyD4I CCNGG 4 cut(s) 315, 420, 482, 753
TaaI ACNGT 2 cut(s) 311, 1363
TaiI ACGT 5 cut(s) 249, 352, 453, 572, 1056
TaqI TCGA 5 cut(s) 198, 249, 429, 577, 1440
TatI WGTACW 2 cut(s) 525, 1110
TfiI GAWTC 2 cut(s) 937, 1282
Tru1I TTAA 2 cut(s) 23, 678
Tru9I TTAA 2 cut(s) 23, 678
TscAI CASTG 4 cut(s) 59, 259, 790, 909
TseFI GTSAC 3 cut(s) 4, 710, 1057
TseI GCWGC 3 cut(s) 91, 986, 1424
Tsp45I GTSAC 3 cut(s) 4, 710, 1057
TspDTI ATGAA 5 cut(s) 522, 914, 1244, 1473, 1496
TspGWI ACGGA 1 cut(s) 856
TspRI CASTG 4 cut(s) 59, 259, 790, 909
Vha464I CTTAAG 1 cut(s) 22
VpaK11BI GGWCC 4 cut(s) 214, 319, 559, 1355
XapI RAATTY 2 cut(s) 469, 921
XceI RCATGY 3 cut(s) 285, 641, 697
XhoI CTCGAG 2 cut(s) 197, 576
XmiI GTMKAC 3 cut(s) 28, 249, 1504
XspI CTAG 3 cut(s) 228, 686, 726
ZraI GACGTC 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.