Rorug01G0385000

Pentatricopeptide repeat-containing protein At4g25270

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0015204
Physical Location & Seq
Reverse (-)
49665196 .. 49670226
5031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0385000.1

Sequence Viewer

Length: 2232 bp
ATGGCTCTTCGATGGTTTCTCCTCCATAGCCTCATTGGCAGTATTCTATTTCTTGTTGATGTTACTCACTCAGTTGCTCGGGATGCCCGAAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGGGATGCCAGTAACACCTGATCTTCATGTAAATATTCTACGAGACATAGTTGATAGCAGCATTAATAATGATATTGCACATGAAGATCTGCTTCTTCACAGCTACAAGAGAAGTTTCCATGGATTTGCTGCAATGCTCACAGAACAAGAATCACAAAAACTGGCTGGAATGGATGGTGTAGTGTCTGTCTTTCCAAGTAAAAAGGTGAGTGTCAAAACAACAACGTCATGGAATTTCGTTGGGTTTCCAGAATCAATTGGGAGCGGCCCCTTTGAAAGTGATATCATTATCGGTGTGCTCGACAGTGGAATTTGGCCGGAGTTGGATAGCTTTAATGATGTTGGTTTTGGTCCGCCACCTAAGAAATGGAAAGGCATATGTCAAGGCAATAACAACTTCACTTGTAACAATAAGATCATCGGAGCACGGTATTACAAAGATAGTGACTTCGAAGAAGGAGAGATTCAGTCTCCAAGAGACTCATCCGGTCATGGAACCCACTGCGCATCAGTAGCAGCTGGGAATGCAGTGAGCAACGCGAGCATAAATGGTTTAGGGTTGGGAACAGCAAGAGGAGCGGTGCCATCAGCACGTATTGCGGTGTACAAAATATGTTGGAAGGATGGTTGTGGTGGTGCAGAGCTTCTAGCGGCAATCGATGATGCCATTGCTGACGGTGTCGACATTCTCAGTGCTTCCACCGGAACCGATGCGCCAGCTGACTTTTTGAATGACGCATTTGCAATTGGGACATTTCACGCTACCGTAAATGGGATATTGGTTTCTATGGCTGCTGGTAATGAAGGTCCGCTAAAAAAAACTGTGGAAAACATTGCACCATGGCAACTTTCTGTGGCTGCTACCACCACAAACCGTCAGTTCATCACCAAGGTTCAATTGGGTAATGGTAAAATCTATGAGGGACTTTCCGTACATCTACATGACCTCAAAGGTAAATTTTACCCGTTAATTTACGGTGGAGATGCACCCAGTAAAAAAGAAGATGGTAAAGGATATGCATCTAGGTTTTGTGTTGGAGATAGCTTAGATGCAGATAAGATCAAAGGTAAAATTGTGCTTTGTGATCGCCCCGATAAGGATGGGAAGGGGGCTGGGGATGGTGCCAAATGGTGGAATGCTTCAATGGGAATTATTCTGACGGGCAAACAAGTTGTCGAGGAAACCGAATATGCTGATAACCCTGTAGCAGCAGCTTACGTCAATTTAGAAGATAATAGCAACATTTACAAATACATAAACAAAACAAGGGACCCAACTGCAACTATTTGGAAAAGTAAAGAGATCAATGATGTATTGGCTCCATACGTGCCCTCCTACTCATCAAGGGGTCCAAACGCGTTGAATCCTAACATACTGAAGCCAGATATAGCGGCTCCGGGAACTTACATTCTAGCAGCATTTCCCCCAAATGTTAGTAATGGAGCTAAATACAAATTGGCAACCGGCACATCAATGGCATGCCCCCATGCTTCGTCTGTAGCTGCGTACGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCTGTCCAATCGGCTCTCATCACTACTGCTACACCTATGAGTGCCAAAACTACCCCGCACGCTGAAATTGCATATGGAGCTGGCCTAATAAACCCTTCCAGAGCTCCATATCCTGGTTTGGTTTACGATCTTGATGTACAAGACTATGTAGATTTTTTGTGTTCACAAGGATACAGTGACAAACTATTAGAACAAATAACCAGGGACAAGACTACCTTATGCTCATCAAAATCTAATAATGAAACAGTCAATGACCTGAATTATCCTTCTTTTTCTCTTCCTATCAAGGATCCGAAATTCGTCAATGGGGTTTTCCACAGGACTGTCACTAATGTCGGATCATCAAATTCTATATACAGAGCTAAAGTGGTGGCTCCATCGGGACTCAAAATCAATGTGGATCCAAGTGTGCTGTCGTTCACATCTCTTGGACAAAAGAAATCTTTTGTAGTCACTGTAAAAGGGCCGATTGAGCAATCAAGTATGGTCTCTGCGTCTTTGGTGTGGGATGATGGTGCTTTCCAAGTCAGGAGCCCCATCGTTGTCTATATTCAACTCTAG

Protein Analysis

743

Amino Acids

79.7

Weight (kDa)

6.58

Isoelectric Point (pI)

31.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 114 2.1e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 585 4.8e-44 Subtilase family
fn3_6 PF17766 642 - 739 7.9e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25270
fragaria_vesca FvH4_7g27870
malus_domestica MD00G1104900.v1.1
prunus_persica Prupe.2G280000_v2.0.a1
pyrus_communis pycom01g19550
rosa_chinensis RchiOBHm_Chr1g0374811
rosa_laevigata RLG00000026713
rosa_multiflora Rmu_co8506051.1_g000002 Rmu_sc0004947.1_g000023
rosa_roxburghii Rroxscaffold_4G00283250
rosa_rugosa Rorug01G0385000
rosa_samantha Rh1AG396500 Rh1BG360600 Rh1CG374000 Rh1DG391400
rosa_wichuraiana Rw1G035150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 634
AccB1I GGYRCC 2 cut(s) 709, 1250
AccB7I CCANNNNNTGG 2 cut(s) 1260, 1784
AccBSI CCGCTC 2 cut(s) 393, 707
AccI GTMKAC 1 cut(s) 810
AccII CGCG 2 cut(s) 668, 1487
AciI CCGC 8 cut(s) 393, 482, 707, 728, 779, 938, 1520, 1727
AclWI GGATC 5 cut(s) 1955, 1968, 2017, 2066, 2079
AcoI YGGCCR 1 cut(s) 443
AcsI RAATTY 5 cut(s) 361, 438, 1085, 1967, 2017
AcuI CTGAAG 1 cut(s) 1526
AfaI GTAC 4 cut(s) 734, 1062, 1637, 1809
AfiI CCNNNNNNNGG 5 cut(s) 128, 900, 1225, 1260, 1784
AflIII ACRYGT 1 cut(s) 1485
AgsI TTSAA 6 cut(s) 404, 859, 1025, 1272, 1492, 2225
AjnI CCWGG 2 cut(s) 1783, 1871
Alw21I GWGCWC 3 cut(s) 429, 556, 1777
Alw26I GTCTC 4 cut(s) 165, 600, 603, 2164
AlwI GGATC 5 cut(s) 1955, 1968, 2017, 2066, 2079
Ama87I CYCGRG 1 cut(s) 78
AoxI GGCC 4 cut(s) 394, 443, 1753, 2135
ApoI RAATTY 5 cut(s) 361, 438, 1085, 1967, 2017
ArsI GACNNNNNNTTYG 2 cut(s) 1287, 1319
AseI ATTAAT 1 cut(s) 192
AspLEI GCGC 2 cut(s) 635, 844
AspS9I GGNCC 6 cut(s) 395, 479, 935, 1399, 1478, 2135
AsuC2I CCSGG 1 cut(s) 1527
AsuHPI GGTGA 4 cut(s) 346, 1006, 1643, 1656
AsuII TTCGAA 1 cut(s) 579
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 4 cut(s) 479, 935, 1399, 1478
BaeGI GKGCMC 1 cut(s) 1461
BamHI GGATCC 2 cut(s) 1960, 2071
BanI GGYRCC 2 cut(s) 709, 1250
BanII GRGCYC 2 cut(s) 1777, 2207
Bbv12I GWGCWC 3 cut(s) 429, 556, 1777
BciT130I CCWGG 2 cut(s) 1785, 1873
BciVI GTATCC 1 cut(s) 1835
BcnI CCSGG 1 cut(s) 1527
BcoDI GTCTC 4 cut(s) 165, 600, 603, 2164
BfaI CTAG 4 cut(s) 776, 1152, 1541, 2230
BfmI CTRYAG 2 cut(s) 1332, 1626
BfuI GTATCC 1 cut(s) 1835
BglI GCCNNNNNGGC 2 cut(s) 36, 92
BglII AGATCT 1 cut(s) 214
Bme1390I CCNGG 3 cut(s) 1527, 1785, 1873
Bme18I GGWCC 4 cut(s) 479, 935, 1399, 1478
BmeT110I CYCGRG 1 cut(s) 78
BmgT120I GGNCC 6 cut(s) 395, 479, 935, 1399, 1478, 2135
BmrFI CCNGG 3 cut(s) 1527, 1785, 1873
BmrI ACTGGG 1 cut(s) 1113
BmsI GCATC 8 cut(s) 73, 123, 644, 781, 829, 1102, 1157, 1168
BmuI ACTGGG 1 cut(s) 1113
Bpu14I TTCGAA 1 cut(s) 579
BpuMI CCSGG 1 cut(s) 1527
Bsa29I ATCGAT 1 cut(s) 786
BsaAI YACGTR 2 cut(s) 722, 1456
BsaBI GATNNNNATC 1 cut(s) 1147
BsaI GGTCTC 1 cut(s) 2164
BsaJI CCNNGG 4 cut(s) 247, 968, 1017, 1872
BsaWI WCCGGW 2 cut(s) 614, 830
BsaXI ACNNNNNCTCC 2 cut(s) 1445, 1475
Bsc4I CCNNNNNNNGG 5 cut(s) 128, 900, 1225, 1260, 1784
Bse118I RCCGGY 2 cut(s) 1592, 1666
Bse1I ACTGG 3 cut(s) 137, 294, 1119
Bse3DI GCAATG 3 cut(s) 267, 795, 960
Bse8I GATNNNNATC 1 cut(s) 1147
BseBI CCWGG 2 cut(s) 1785, 1873
BseCI ATCGAT 1 cut(s) 786
BseDI CCNNGG 4 cut(s) 247, 968, 1017, 1872
BseGI GGATG 8 cut(s) 88, 138, 307, 611, 757, 1234, 1252, 2185
BseJI GATNNNNATC 1 cut(s) 1147
BseLI CCNNNNNNNGG 5 cut(s) 128, 900, 1225, 1260, 1784
BseMI GCAATG 3 cut(s) 267, 795, 960
BseMII CTCAG 2 cut(s) 84, 832
BseNI ACTGG 3 cut(s) 137, 294, 1119
BseRI GAGGAG 2 cut(s) 11, 717
BseSI GKGCMC 1 cut(s) 1461
BseYI CCCAGC 2 cut(s) 647, 1241
BsgI GTGCAG 1 cut(s) 786
Bsh1236I CGCG 2 cut(s) 668, 1487
BshFI GGCC 4 cut(s) 396, 445, 1755, 2137
BshNI GGYRCC 2 cut(s) 709, 1250
BshVI ATCGAT 1 cut(s) 786
BsiHKAI GWGCWC 3 cut(s) 429, 556, 1777
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 6 cut(s) 446, 615, 831, 1526, 1593, 1667
BsiWI CGTACG 1 cut(s) 1635
BslFI GGGAC 5 cut(s) 892, 1065, 1412, 1889, 2067
BslI CCNNNNNNNGG 5 cut(s) 128, 900, 1225, 1260, 1784
BsmAI GTCTC 4 cut(s) 165, 600, 603, 2164
BsmFI GGGAC 5 cut(s) 892, 1065, 1412, 1889, 2067
BsmI GAATGC 2 cut(s) 658, 1270
BsnI GGCC 4 cut(s) 396, 445, 1755, 2137
Bso31I GGTCTC 1 cut(s) 2164
BsoBI CYCGRG 1 cut(s) 78
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 5 cut(s) 429, 556, 1461, 1777, 2207
Bsp1407I TGTACA 2 cut(s) 732, 1807
Bsp19I CCATGG 2 cut(s) 247, 968
BspACI CCGC 8 cut(s) 393, 482, 707, 728, 779, 938, 1520, 1727
BspANI GGCC 4 cut(s) 396, 445, 1755, 2137
BspCNI CTCAG 2 cut(s) 83, 831
BspDI ATCGAT 1 cut(s) 786
BspFNI CGCG 2 cut(s) 668, 1487
BspPI GGATC 5 cut(s) 1955, 1968, 2017, 2066, 2079
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 2 cut(s) 709, 1250
BspTNI GGTCTC 1 cut(s) 2164
BsrBI CCGCTC 2 cut(s) 393, 707
BsrDI GCAATG 3 cut(s) 267, 795, 960
BsrFI RCCGGY 2 cut(s) 1592, 1666
BsrGI TGTACA 2 cut(s) 732, 1807
BsrI ACTGG 3 cut(s) 137, 294, 1119
BssAI RCCGGY 2 cut(s) 1592, 1666
BssECI CCNNGG 4 cut(s) 247, 968, 1017, 1872
BssT1I CCWWGG 3 cut(s) 247, 968, 1017
Bst2UI CCWGG 2 cut(s) 1785, 1873
Bst6I CTCTTC 2 cut(s) 12, 1953
BstAPI GCANNNNNTGC 1 cut(s) 725
BstAUI TGTACA 2 cut(s) 732, 1807
BstBAI YACGTR 2 cut(s) 722, 1456
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 5 cut(s) 670, 846, 1609, 1731, 1753
BstDEI CTNAG 4 cut(s) 70, 489, 818, 1174
BstDSI CCRYGG 2 cut(s) 247, 968
BstEII GGTNACC 1 cut(s) 1662
BstF5I GGATG 8 cut(s) 88, 138, 307, 611, 757, 1234, 1252, 2185
BstFNI CGCG 2 cut(s) 668, 1487
BstHHI GCGC 2 cut(s) 635, 844
BstMAI GTCTC 4 cut(s) 165, 600, 603, 2164
BstNI CCWGG 2 cut(s) 1785, 1873
BstNSI RCATGY 1 cut(s) 1611
BstPI GGTNACC 1 cut(s) 1662
BstSCI CCNGG 3 cut(s) 1525, 1783, 1871
BstSFI CTRYAG 2 cut(s) 1332, 1626
BstSLI GKGCMC 1 cut(s) 1461
BstUI CGCG 2 cut(s) 668, 1487
BstX2I RGATCY 3 cut(s) 214, 1960, 2071
BstYI RGATCY 3 cut(s) 214, 1960, 2071
Bsu15I ATCGAT 1 cut(s) 786
BsuI GTATCC 1 cut(s) 1835
BsuRI GGCC 4 cut(s) 396, 445, 1755, 2137
BsuTUI ATCGAT 1 cut(s) 786
BtgI CCRYGG 2 cut(s) 247, 968
BtsCI GGATG 8 cut(s) 88, 138, 307, 611, 757, 1234, 1252, 2185
BtsI GCAGTG 2 cut(s) 628, 663
BtsIMutI CAGTG 6 cut(s) 439, 628, 663, 826, 1852, 2124
Cac8I GCNNGC 5 cut(s) 670, 846, 1609, 1731, 1753
CfoI GCGC 2 cut(s) 635, 844
Cfr10I RCCGGY 2 cut(s) 1592, 1666
Cfr13I GGNCC 6 cut(s) 395, 479, 935, 1399, 1478, 2135
ClaI ATCGAT 1 cut(s) 786
CseI GACGC 2 cut(s) 872, 2154
Csp6I GTAC 4 cut(s) 733, 1061, 1636, 1808
CviAII CATG 9 cut(s) 155, 209, 248, 357, 620, 969, 1070, 1608, 1616
CviQI GTAC 4 cut(s) 733, 1061, 1636, 1808
DdeI CTNAG 4 cut(s) 70, 489, 818, 1174
EaeI YGGCCR 1 cut(s) 443
Eam1104I CTCTTC 2 cut(s) 12, 1953
EarI CTCTTC 2 cut(s) 12, 1953
EciI GGCGGA 1 cut(s) 471
Ecl136II GAGCTC 1 cut(s) 1775
Eco130I CCWWGG 3 cut(s) 247, 968, 1017
Eco24I GRGCYC 2 cut(s) 1777, 2207
Eco31I GGTCTC 1 cut(s) 2164
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 4 cut(s) 479, 935, 1399, 1478
Eco53kI GAGCTC 1 cut(s) 1775
Eco57I CTGAAG 1 cut(s) 1526
Eco88I CYCGRG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 1662
EcoICRI GAGCTC 1 cut(s) 1775
EcoO109I RGGNCCY 1 cut(s) 1399
EcoO65I GGTNACC 1 cut(s) 1662
EcoRII CCWGG 2 cut(s) 1783, 1871
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 3 cut(s) 247, 968, 1017
EcoT22I ATGCAT 1 cut(s) 1150
EcoT38I GRGCYC 2 cut(s) 1777, 2207
ErhI CCWWGG 3 cut(s) 247, 968, 1017
FaeI CATG 9 cut(s) 158, 212, 251, 360, 623, 972, 1073, 1611, 1619
FalI AAGNNNNNCTT 4 cut(s) 204, 236, 1871, 1903
FaqI GGGAC 5 cut(s) 892, 1065, 1412, 1889, 2067
FatI CATG 9 cut(s) 154, 208, 247, 356, 619, 968, 1069, 1607, 1615
FauI CCCGC 1 cut(s) 1734
FauNDI CATATG 2 cut(s) 506, 1744
FblI GTMKAC 1 cut(s) 810
FokI GGATG 8 cut(s) 95, 145, 314, 598, 764, 1241, 1259, 2192
FriOI GRGCYC 2 cut(s) 1777, 2207
FspBI CTAG 4 cut(s) 776, 1152, 1541, 2230
FspI TGCGCA 1 cut(s) 634
GlaI GCGC 2 cut(s) 634, 843
GsaI CCCAGC 2 cut(s) 651, 1245
HaeIII GGCC 4 cut(s) 396, 445, 1755, 2137
HapII CCGG 6 cut(s) 446, 615, 831, 1526, 1593, 1667
HgaI GACGC 2 cut(s) 872, 2154
HhaI GCGC 2 cut(s) 635, 844
Hin1II CATG 9 cut(s) 158, 212, 251, 360, 623, 972, 1073, 1611, 1619
Hin6I GCGC 2 cut(s) 633, 842
HinP1I GCGC 2 cut(s) 633, 842
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HinfI GANTC 6 cut(s) 278, 380, 592, 608, 1492, 2055
HpaII CCGG 6 cut(s) 446, 615, 831, 1526, 1593, 1667
HphI GGTGA 4 cut(s) 346, 1006, 1643, 1656
Hpy166II GTNNAC 5 cut(s) 733, 811, 1795, 1835, 2091
Hpy188I TCNGA 4 cut(s) 551, 1287, 1965, 2009
Hpy188III TCNNGA 6 cut(s) 80, 377, 1770, 1802, 2052, 2200
Hpy8I GTNNAC 5 cut(s) 733, 811, 1795, 1835, 2091
HpyAV CCTTC 6 cut(s) 578, 742, 926, 1228, 1776, 1947
HpyCH4IV ACGT 5 cut(s) 353, 721, 1347, 1455, 1638
HpyF3I CTNAG 4 cut(s) 70, 489, 818, 1174
HpySE526I ACGT 5 cut(s) 353, 721, 1347, 1455, 1638
Hsp92II CATG 9 cut(s) 158, 212, 251, 360, 623, 972, 1073, 1611, 1619
HspAI GCGC 2 cut(s) 633, 842
KflI GGGWCCC 1 cut(s) 1399
LguI GCTCTTC 1 cut(s) 12
LweI GCATC 8 cut(s) 73, 123, 644, 781, 829, 1102, 1157, 1168
MaeI CTAG 4 cut(s) 776, 1152, 1541, 2230
MaeII ACGT 5 cut(s) 353, 721, 1347, 1455, 1638
MaeIII GTNAC 8 cut(s) 61, 139, 533, 572, 1662, 1847, 1996, 2122
MbiI CCGCTC 2 cut(s) 393, 707
MboII GAAGA 7 cut(s) 143, 215, 224, 593, 1142, 1370, 1940
MfeI CAATTG 3 cut(s) 384, 873, 1025
MflI RGATCY 3 cut(s) 214, 1960, 2071
MhlI GDGCHC 5 cut(s) 429, 556, 1461, 1777, 2207
MluI ACGCGT 1 cut(s) 1485
MlyI GAGTC 2 cut(s) 602, 2049
MmeI TCCRAC 4 cut(s) 432, 725, 1144, 1987
MnlI CCTC 7 cut(s) 32, 41, 695, 1042, 1085, 1300, 1471
Mph1103I ATGCAT 1 cut(s) 1150
MseI TTAA 3 cut(s) 192, 462, 1097
MslI CAYNNNNRTG 2 cut(s) 1068, 1601
MspA1I CMGCKG 2 cut(s) 647, 848
MspI CCGG 6 cut(s) 446, 615, 831, 1526, 1593, 1667
MspR9I CCNGG 3 cut(s) 1527, 1785, 1873
MunI CAATTG 3 cut(s) 384, 873, 1025
Mva1269I GAATGC 2 cut(s) 658, 1270
MvaI CCWGG 2 cut(s) 1785, 1873
MvnI CGCG 2 cut(s) 668, 1487
NciI CCSGG 1 cut(s) 1527
NcoI CCATGG 2 cut(s) 247, 968
NdeI CATATG 2 cut(s) 506, 1744
NlaIII CATG 9 cut(s) 158, 212, 251, 360, 623, 972, 1073, 1611, 1619
NmuCI GTSAC 5 cut(s) 572, 1662, 1847, 1996, 2122
NsbI TGCGCA 1 cut(s) 634
NsiI ATGCAT 1 cut(s) 1150
NspI RCATGY 1 cut(s) 1611
NspV TTCGAA 1 cut(s) 579
PaeI GCATGC 1 cut(s) 1611
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 3 cut(s) 85, 426, 1311
PctI GAATGC 2 cut(s) 658, 1270
PfeI GAWTC 4 cut(s) 278, 380, 592, 1492
Pfl23II CGTACG 1 cut(s) 1635
PflFI GACNNNGTC 1 cut(s) 806
PflMI CCANNNNNTGG 2 cut(s) 1260, 1784
PfoI TCCNGGA 1 cut(s) 1525
PleI GAGTC 2 cut(s) 602, 2049
PpsI GAGTC 2 cut(s) 602, 2049
Ppu21I YACGTR 2 cut(s) 722, 1456
PpuMI RGGWCCY 1 cut(s) 1399
PshBI ATTAAT 1 cut(s) 192
Psp124BI GAGCTC 1 cut(s) 1777
Psp5II RGGWCCY 1 cut(s) 1399
Psp6I CCWGG 2 cut(s) 1783, 1871
PspEI GGTNACC 1 cut(s) 1662
PspFI CCCAGC 2 cut(s) 647, 1241
PspGI CCWGG 2 cut(s) 1783, 1871
PspLI CGTACG 1 cut(s) 1635
PspPI GGNCC 6 cut(s) 395, 479, 935, 1399, 1478, 2135
PspPPI RGGWCCY 1 cut(s) 1399
PsuI RGATCY 3 cut(s) 214, 1960, 2071
PsyI GACNNNGTC 1 cut(s) 806
PvuII CAGCTG 2 cut(s) 647, 848
RsaI GTAC 4 cut(s) 734, 1062, 1637, 1809
RsaNI GTAC 4 cut(s) 733, 1061, 1636, 1808
RseI CAYNNNNRTG 2 cut(s) 1068, 1601
SacI GAGCTC 1 cut(s) 1777
SalI GTCGAC 1 cut(s) 809
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 192, 462, 1097
Sau96I GGNCC 6 cut(s) 395, 479, 935, 1399, 1478, 2135
SchI GAGTC 2 cut(s) 602, 2049
ScrFI CCNGG 3 cut(s) 1527, 1785, 1873
SduI GDGCHC 5 cut(s) 429, 556, 1461, 1777, 2207
SfaNI GCATC 8 cut(s) 73, 123, 644, 781, 829, 1102, 1157, 1168
SfcI CTRYAG 2 cut(s) 1332, 1626
SfuI TTCGAA 1 cut(s) 579
SinI GGWCC 4 cut(s) 479, 935, 1399, 1478
SmiMI CAYNNNNRTG 2 cut(s) 1068, 1601
SphI GCATGC 1 cut(s) 1611
SsiI CCGC 8 cut(s) 393, 482, 707, 728, 779, 938, 1520, 1727
SspI AATATT 1 cut(s) 163
SspMI CTAG 4 cut(s) 776, 1152, 1541, 2230
SstI GAGCTC 1 cut(s) 1777
StyD4I CCNGG 3 cut(s) 1525, 1783, 1871
StyI CCWWGG 3 cut(s) 247, 968, 1017
TaiI ACGT 5 cut(s) 356, 724, 1350, 1458, 1641
TaqI TCGA 6 cut(s) 10, 429, 579, 786, 810, 1305
TatI WGTACW 2 cut(s) 732, 1807
TauI GCSGC 3 cut(s) 396, 782, 1523
TfiI GAWTC 4 cut(s) 278, 380, 592, 1492
Tru1I TTAA 3 cut(s) 192, 462, 1097
Tru9I TTAA 3 cut(s) 192, 462, 1097
TscAI CASTG 6 cut(s) 439, 635, 663, 826, 1852, 2131
TseFI GTSAC 5 cut(s) 572, 1662, 1847, 1996, 2122
Tsp45I GTSAC 5 cut(s) 572, 1662, 1847, 1996, 2122
TspDTI ATGAA 5 cut(s) 143, 225, 945, 1000, 1926
TspGWI ACGGA 1 cut(s) 1048
TspRI CASTG 6 cut(s) 439, 635, 663, 826, 1852, 2131
Tth111I GACNNNGTC 1 cut(s) 806
Van91I CCANNNNNTGG 2 cut(s) 1260, 1784
VpaK11BI GGWCC 4 cut(s) 479, 935, 1399, 1478
VspI ATTAAT 1 cut(s) 192
XapI RAATTY 5 cut(s) 361, 438, 1085, 1967, 2017
XceI RCATGY 1 cut(s) 1611
XcmI CCANNNNNNNNNTGG 3 cut(s) 32, 492, 1024
XmiI GTMKAC 1 cut(s) 810
XspI CTAG 4 cut(s) 776, 1152, 1541, 2230
Zsp2I ATGCAT 1 cut(s) 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.