AT4G25780

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0017047
Physical Location & Seq
Forward (+)
13120824 .. 13122042
1219 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25780.1

Sequence Viewer

Length: 573 bp
ATGAGCTCATCCTATTTACGAGTAATATTACTCTTAGGTGCCCTTAATGTCGTCGTTTCTTTATCAATAACCAATTCTTTAATAACCAAATCAGCAACACTCGGTCAGGTGTTCCGAATCTGTAAGAATTTGTGTCCGGGATGTGACCACGACTCCTTGCAGTTCTTGTTCCGACACAACCTGGTCCGTGCCGCAAGATTCGAACCTCCTTTGATATGGGACCGCAGACTCCAGAACTACGCCCAAGGGTGGGCTAATCAAAGAAGGGGAGATTGTGCTTTGAGACACTCTGTCTCAAACGGAGAGTTTAATCTCGGCGAGAACATCTACTGGGGATACGGCGCCAACTGGTCTCCGGCCGACGCGGTGGTTGCATGGGCCAGCGAGAAGAGGTTTTATCATTACGGCTCCAACACGTGCGACGCTGGTCAGATGTGTGGTCATTACACACAGATTGTGTGGAAGAGCACTAGGAGGGTCGGATGTGCACGTGTGGTGTGCGACAATGGTGGGATCTTCATGACTTGTAACTATGATCCTCCCGGTAACTACATCGGCCAGAAACCCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP

Protein Analysis

190

Amino Acids

21.38

Weight (kDa)

9.02

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 58 - 178 3.9e-20 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25780
fragaria_vesca FvH4_4g09940
malus_domestica MD13G1265800.v1.1
prunus_persica Prupe.1G091400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0403511
rosa_laevigata RLG00000008971
rosa_multiflora Rmu_sc0007727.1_g000036
rosa_roxburghii Rroxscaffold_5G00348180
rosa_rugosa Rorug04G0043900
rosa_samantha Rh4AG119300 Rh4CG127200 Rh4DG112000
rosa_wichuraiana Rw4G009680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 38, 341
AccII CGCG 1 cut(s) 365
AciI CCGC 3 cut(s) 192, 223, 365
AclWI GGATC 2 cut(s) 521, 530
AcoI YGGCCR 2 cut(s) 357, 556
AcsI RAATTY 1 cut(s) 127
AcvI CACGTG 2 cut(s) 417, 491
AcyI GRCGYC 1 cut(s) 342
AfiI CCNNNNNNNGG 2 cut(s) 249, 250
AflIII ACRYGT 2 cut(s) 414, 490
AhdI GACNNNNNGTC 1 cut(s) 290
AjnI CCWGG 1 cut(s) 180
AluBI AGCT 1 cut(s) 6
AluI AGCT 1 cut(s) 6
Alw21I GWGCWC 3 cut(s) 8, 470, 490
Alw26I GTCTC 3 cut(s) 277, 298, 357
Alw44I GTGCAC 1 cut(s) 486
AlwI GGATC 2 cut(s) 521, 530
AoxI GGCC 3 cut(s) 357, 378, 556
ApaLI GTGCAC 1 cut(s) 486
ApoI RAATTY 1 cut(s) 127
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 3 cut(s) 184, 220, 378
AsuC2I CCSGG 2 cut(s) 138, 543
AsuII TTCGAA 1 cut(s) 201
AvaII GGWCC 2 cut(s) 184, 220
BaeGI GKGCMC 2 cut(s) 43, 490
BanI GGYRCC 2 cut(s) 38, 341
BanII GRGCYC 1 cut(s) 8
BbrPI CACGTG 2 cut(s) 417, 491
Bbv12I GWGCWC 3 cut(s) 8, 470, 490
BceAI ACGGC 2 cut(s) 355, 421
BciT130I CCWGG 1 cut(s) 182
BciVI GTATCC 1 cut(s) 329
BcnI CCSGG 2 cut(s) 138, 543
BcoDI GTCTC 3 cut(s) 277, 298, 357
BfaI CTAG 1 cut(s) 471
BfoI RGCGCY 1 cut(s) 345
BfuI GTATCC 1 cut(s) 329
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
Bme1390I CCNGG 3 cut(s) 138, 182, 543
Bme18I GGWCC 2 cut(s) 184, 220
BmeRI GACNNNNNGTC 1 cut(s) 290
BmgT120I GGNCC 3 cut(s) 184, 220, 378
BmiI GGNNCC 4 cut(s) 40, 221, 343, 409
BmrFI CCNGG 3 cut(s) 138, 182, 543
BmrI ACTGGG 1 cut(s) 340
BmuI ACTGGG 1 cut(s) 340
BoxI GACNNNNGTC 1 cut(s) 426
BpmI CTGGAG 1 cut(s) 215
Bpu14I TTCGAA 1 cut(s) 201
BpuMI CCSGG 2 cut(s) 138, 543
BsaAI YACGTR 2 cut(s) 417, 491
BsaHI GRCGYC 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 357
BsaJI CCNNGG 1 cut(s) 244
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 250
Bse1I ACTGG 2 cut(s) 335, 353
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 3 cut(s) 8, 146, 488
BseLI CCNNNNNNNGG 2 cut(s) 249, 250
BseNI ACTGG 2 cut(s) 335, 353
BseSI GKGCMC 2 cut(s) 43, 490
BseX3I CGGCCG 1 cut(s) 357
Bsh1236I CGCG 1 cut(s) 365
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 3 cut(s) 359, 380, 558
BshNI GGYRCC 2 cut(s) 38, 341
BsiEI CGRYCG 1 cut(s) 360
BsiHKAI GWGCWC 3 cut(s) 8, 470, 490
BsiSI CCGG 3 cut(s) 137, 356, 543
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 2 cut(s) 249, 250
BsmAI GTCTC 3 cut(s) 277, 298, 357
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 3 cut(s) 359, 380, 558
Bso31I GGTCTC 1 cut(s) 357
Bsp119I TTCGAA 1 cut(s) 201
Bsp1286I GDGCHC 4 cut(s) 8, 43, 470, 490
Bsp143I GATC 2 cut(s) 513, 535
BspACI CCGC 3 cut(s) 192, 223, 365
BspANI GGCC 3 cut(s) 359, 380, 558
BspFNI CGCG 1 cut(s) 365
BspHI TCATGA 1 cut(s) 519
BspLI GGNNCC 4 cut(s) 40, 221, 343, 409
BspPI GGATC 2 cut(s) 521, 530
BspQI GCTCTTC 1 cut(s) 458
BspT104I TTCGAA 1 cut(s) 201
BspT107I GGYRCC 2 cut(s) 38, 341
BspTNI GGTCTC 1 cut(s) 357
BsrI ACTGG 2 cut(s) 335, 353
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 2 cut(s) 513, 535
BssNI GRCGYC 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 182
Bst6I CTCTTC 2 cut(s) 383, 458
BstACI GRCGYC 1 cut(s) 342
BstBAI YACGTR 2 cut(s) 417, 491
BstBI TTCGAA 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 382
BstDEI CTNAG 1 cut(s) 34
BstF5I GGATG 3 cut(s) 8, 146, 488
BstFNI CGCG 1 cut(s) 365
BstH2I RGCGCY 1 cut(s) 345
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 2 cut(s) 516, 538
BstMAI GTCTC 3 cut(s) 277, 298, 357
BstMBI GATC 2 cut(s) 513, 535
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 1 cut(s) 371
BstNI CCWGG 1 cut(s) 182
BstPAI GACNNNNGTC 1 cut(s) 426
BstSCI CCNGG 3 cut(s) 136, 180, 541
BstSLI GKGCMC 2 cut(s) 43, 490
BstUI CGCG 1 cut(s) 365
BstX2I RGATCY 1 cut(s) 513
BstYI RGATCY 1 cut(s) 513
BstZI CGGCCG 1 cut(s) 357
BsuI GTATCC 1 cut(s) 329
BsuRI GGCC 3 cut(s) 359, 380, 558
BtsCI GGATG 3 cut(s) 8, 146, 488
Cac8I GCNNGC 1 cut(s) 382
CciI TCATGA 1 cut(s) 519
CfoI GCGC 1 cut(s) 344
Cfr13I GGNCC 3 cut(s) 184, 220, 378
CseI GACGC 2 cut(s) 371, 431
CsiI ACCWGGT 1 cut(s) 180
CviAII CATG 2 cut(s) 375, 520
CviJI RGCY 6 cut(s) 6, 254, 359, 380, 408, 558
CviKI_1 RGCY 6 cut(s) 6, 254, 359, 380, 408, 558
DdeI CTNAG 1 cut(s) 34
DinI GGCGCC 1 cut(s) 343
DpnI GATC 2 cut(s) 515, 537
DpnII GATC 2 cut(s) 513, 535
DriI GACNNNNNGTC 1 cut(s) 290
EaeI YGGCCR 2 cut(s) 357, 556
EagI CGGCCG 1 cut(s) 357
Eam1104I CTCTTC 2 cut(s) 383, 458
Eam1105I GACNNNNNGTC 1 cut(s) 290
EarI CTCTTC 2 cut(s) 383, 458
Ecl136II GAGCTC 1 cut(s) 6
EclXI CGGCCG 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 8
Eco31I GGTCTC 1 cut(s) 357
Eco47I GGWCC 2 cut(s) 184, 220
Eco52I CGGCCG 1 cut(s) 357
Eco53kI GAGCTC 1 cut(s) 6
Eco72I CACGTG 2 cut(s) 417, 491
EcoICRI GAGCTC 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 180
EcoT14I CCWWGG 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 8
EgeI GGCGCC 1 cut(s) 343
EheI GGCGCC 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 2 cut(s) 378, 523
FaiI YATR 4 cut(s) 217, 376, 521, 534
FaqI GGGAC 1 cut(s) 233
FatI CATG 2 cut(s) 374, 519
Fnu4HI GCNGC 1 cut(s) 192
FokI GGATG 2 cut(s) 153, 495
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 1 cut(s) 192
FspBI CTAG 1 cut(s) 471
GlaI GCGC 1 cut(s) 343
GluI GCNGC 1 cut(s) 192
GsuI CTGGAG 1 cut(s) 215
HaeII RGCGCY 1 cut(s) 345
HaeIII GGCC 3 cut(s) 359, 380, 558
HapII CCGG 3 cut(s) 137, 356, 543
HgaI GACGC 2 cut(s) 371, 431
HhaI GCGC 1 cut(s) 344
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 2 cut(s) 378, 523
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HinfI GANTC 4 cut(s) 117, 152, 198, 228
HpaII CCGG 3 cut(s) 137, 356, 543
Hpy166II GTNNAC 1 cut(s) 488
Hpy188I TCNGA 4 cut(s) 116, 173, 432, 482
Hpy188III TCNNGA 2 cut(s) 232, 520
Hpy8I GTNNAC 1 cut(s) 488
Hpy99I CGWCG 3 cut(s) 56, 365, 425
HpyAV CCTTC 1 cut(s) 258
HpyCH4IV ACGT 2 cut(s) 416, 490
HpyCH4V TGCA 3 cut(s) 160, 374, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 371
HpyF3I CTNAG 1 cut(s) 34
HpySE526I ACGT 2 cut(s) 416, 490
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 2 cut(s) 378, 523
HspAI GCGC 1 cut(s) 342
KasI GGCGCC 1 cut(s) 341
Kzo9I GATC 2 cut(s) 513, 535
LguI GCTCTTC 1 cut(s) 458
LmnI GCTCC 1 cut(s) 413
MabI ACCWGGT 1 cut(s) 180
MaeI CTAG 1 cut(s) 471
MaeII ACGT 2 cut(s) 416, 490
MaeIII GTNAC 3 cut(s) 143, 527, 545
MalI GATC 2 cut(s) 515, 537
MboI GATC 2 cut(s) 513, 535
MboII GAAGA 3 cut(s) 400, 475, 508
MflI RGATCY 1 cut(s) 513
MhlI GDGCHC 4 cut(s) 8, 43, 470, 490
MluCI AATT 2 cut(s) 73, 127
Mly113I GGCGCC 1 cut(s) 342
MlyI GAGTC 2 cut(s) 146, 222
MmeI TCCRAC 3 cut(s) 196, 435, 460
MnlI CCTC 4 cut(s) 216, 384, 468, 549
MseI TTAA 4 cut(s) 45, 80, 309, 571
MspI CCGG 3 cut(s) 137, 356, 543
MspR9I CCNGG 3 cut(s) 138, 182, 543
MvaI CCWGG 1 cut(s) 182
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 1 cut(s) 371
NarI GGCGCC 1 cut(s) 342
NciI CCSGG 2 cut(s) 138, 543
NdeII GATC 2 cut(s) 513, 535
NlaIII CATG 2 cut(s) 378, 523
NlaIV GGNNCC 4 cut(s) 40, 221, 343, 409
NmeAIII GCCGAG 1 cut(s) 294
NmuCI GTSAC 1 cut(s) 143
NspV TTCGAA 1 cut(s) 201
PagI TCATGA 1 cut(s) 519
PciSI GCTCTTC 1 cut(s) 458
PfeI GAWTC 2 cut(s) 117, 198
PfoI TCCNGGA 1 cut(s) 136
PkrI GCNGC 1 cut(s) 193
PleI GAGTC 2 cut(s) 146, 222
PluTI GGCGCC 1 cut(s) 345
PmaCI CACGTG 2 cut(s) 417, 491
PmlI CACGTG 2 cut(s) 417, 491
PpsI GAGTC 2 cut(s) 146, 222
Ppu21I YACGTR 2 cut(s) 417, 491
PshAI GACNNNNGTC 1 cut(s) 426
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 180
PspCI CACGTG 2 cut(s) 417, 491
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 4 cut(s) 40, 221, 343, 409
PspPI GGNCC 3 cut(s) 184, 220, 378
PsuI RGATCY 1 cut(s) 513
SacI GAGCTC 1 cut(s) 8
SapI GCTCTTC 1 cut(s) 458
SaqAI TTAA 4 cut(s) 45, 80, 309, 571
SatI GCNGC 1 cut(s) 192
Sau3AI GATC 2 cut(s) 513, 535
Sau96I GGNCC 3 cut(s) 184, 220, 378
SchI GAGTC 2 cut(s) 146, 222
ScrFI CCNGG 3 cut(s) 138, 182, 543
SduI GDGCHC 4 cut(s) 8, 43, 470, 490
SetI ASST 8 cut(s) 8, 40, 111, 183, 208, 395, 419, 493
SexAI ACCWGGT 1 cut(s) 180
SfoI GGCGCC 1 cut(s) 343
SfuI TTCGAA 1 cut(s) 201
SinI GGWCC 2 cut(s) 184, 220
Sse9I AATT 2 cut(s) 73, 127
SsiI CCGC 3 cut(s) 192, 223, 365
SspDI GGCGCC 1 cut(s) 341
SspI AATATT 1 cut(s) 27
SspMI CTAG 1 cut(s) 471
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 136, 180, 541
StyI CCWWGG 1 cut(s) 244
TaiI ACGT 2 cut(s) 419, 493
TaqI TCGA 1 cut(s) 201
TaqII GACCGA 1 cut(s) 92
TasI AATT 2 cut(s) 73, 127
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 2 cut(s) 117, 198
Tru1I TTAA 4 cut(s) 45, 80, 309, 571
Tru9I TTAA 4 cut(s) 45, 80, 309, 571
TseFI GTSAC 1 cut(s) 143
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 1 cut(s) 508
TspGWI ACGGA 2 cut(s) 176, 315
VneI GTGCAC 1 cut(s) 486
VpaK11BI GGWCC 2 cut(s) 184, 220
XapI RAATTY 1 cut(s) 127
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.