FvH4_4g09940

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017047
Physical Location & Seq
Forward (+)
12254391 .. 12255493
1103 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g09940.t1

Sequence Viewer

Length: 570 bp
ATGAAGGCACTACTTTTTCCCTTGATAATTCTCTTTGCTCTCTGCACTGTTCAGGCATTTTCTCAATCACCTGGAGGTCATCCGCAAAAGCCCAACAACGACACCATCCTGAGGATCTCCAAGCAACTATGCTGGGGCTGCATTGGAGAGTCACTCCAGTTCTTGTTCCAACACAACATGGTTAGAGCAGCAAAGTGGGAGATGCCACTCATGTGGGATTTCCAGCTCGAGAGGTACGCACGGTGGTGGGCCGGCATCCGAAAAGCCGACTGCAAGCTGCAGCATTCGTTCCCGGAAGACGGGTTCAAGCTGGGAGAGAATATTTACTGGGGCAGCGGCTCGACGTGGACTCCGATCGACGCCGTGAATACCTGGGCTGCTGAGGAGAAGTACTACACTTATGCAACGAATACGTGCGAGGCGGGTCAAATGTGCGGGCACTATACTCAGATTGTGTGGAAGAGTACGAGGAGAGTCGGGTGTGCTAGGGTTCTGTGTGATGATGGAGATGTGTTTATGACTTGTAACTATGATCCGCCTGGTAATTATATTGGTGAAAAGCCTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP

Protein Analysis

190

Amino Acids

21.71

Weight (kDa)

6.87

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 57 - 177 2.5e-19 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25780
fragaria_vesca FvH4_4g09940
malus_domestica MD13G1265800.v1.1
prunus_persica Prupe.1G091400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0403511
rosa_laevigata RLG00000008971
rosa_multiflora Rmu_sc0007727.1_g000036
rosa_roxburghii Rroxscaffold_5G00348180
rosa_rugosa Rorug04G0043900
rosa_samantha Rh4AG119300 Rh4CG127200 Rh4DG112000
rosa_wichuraiana Rw4G009680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 83, 336, 422, 435, 536
AclWI GGATC 2 cut(s) 122, 527
AcyI GRCGYC 1 cut(s) 360
AfaI GTAC 3 cut(s) 236, 392, 466
AfiI CCNNNNNNNGG 2 cut(s) 111, 299
AgsI TTSAA 1 cut(s) 307
AjiI CACGTC 1 cut(s) 345
AjnI CCWGG 3 cut(s) 70, 371, 538
AleI CACNNNNGTG 2 cut(s) 211, 244
AloI GAACNNNNNNTCC 2 cut(s) 287, 319
AluBI AGCT 3 cut(s) 226, 277, 310
AluI AGCT 3 cut(s) 226, 277, 310
AlwI GGATC 2 cut(s) 122, 527
Ama87I CYCGRG 1 cut(s) 227
AoxI GGCC 1 cut(s) 249
ApeKI GCWGC 6 cut(s) 138, 188, 277, 280, 333, 377
AspS9I GGNCC 1 cut(s) 249
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 2 cut(s) 60, 566
AvaI CYCGRG 1 cut(s) 227
AxyI CCTNAGG 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 441
BbsI GAAGAC 1 cut(s) 303
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 6 cut(s) 125, 200, 264, 292, 345, 364
BccI CCATC 2 cut(s) 113, 497
BceAI ACGGC 1 cut(s) 347
BciT130I CCWGG 3 cut(s) 72, 373, 540
BcnI CCSGG 1 cut(s) 293
BfaI CTAG 1 cut(s) 486
BfmI CTRYAG 1 cut(s) 278
BisI GCNGC 7 cut(s) 139, 189, 278, 281, 334, 337, 378
BlsI GCNGC 7 cut(s) 140, 190, 279, 282, 335, 338, 379
BmcAI AGTACT 1 cut(s) 392
Bme1390I CCNGG 4 cut(s) 72, 293, 373, 540
BmeT110I CYCGRG 1 cut(s) 227
BmgBI CACGTC 1 cut(s) 345
BmgT120I GGNCC 1 cut(s) 249
BmrFI CCNGG 4 cut(s) 72, 293, 373, 540
BmrI ACTGGG 1 cut(s) 337
BmsI GCATC 2 cut(s) 192, 264
BmuI ACTGGG 1 cut(s) 337
BpiI GAAGAC 1 cut(s) 303
BplI GAGNNNNNCTC 2 cut(s) 138, 170
BpmI CTGGAG 2 cut(s) 93, 140
Bpu10I CCTNAGC 1 cut(s) 381
BpuMI CCSGG 1 cut(s) 293
BsaAI YACGTR 1 cut(s) 414
BsaHI GRCGYC 1 cut(s) 360
BsaJI CCNNGG 1 cut(s) 372
BsaXI ACNNNNNCTCC 2 cut(s) 334, 364
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 299
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 2 cut(s) 157, 332
Bse21I CCTNAGG 1 cut(s) 110
BseBI CCWGG 3 cut(s) 72, 373, 540
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 3 cut(s) 79, 105, 255
BseLI CCNNNNNNNGG 2 cut(s) 111, 299
BseMII CTCAG 3 cut(s) 101, 372, 461
BseNI ACTGG 2 cut(s) 157, 332
BseRI GAGGAG 2 cut(s) 398, 484
BseSI GKGCMC 1 cut(s) 441
BseXI GCAGC 6 cut(s) 125, 200, 264, 292, 345, 364
BseYI CCCAGC 2 cut(s) 132, 310
BsgI GTGCAG 1 cut(s) 28
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 1 cut(s) 251
BsiEI CGRYCG 1 cut(s) 357
BsiHKCI CYCGRG 1 cut(s) 227
BsiSI CCGG 2 cut(s) 252, 293
BslI CCNNNNNNNGG 2 cut(s) 111, 299
BsmI GAATGC 1 cut(s) 283
BsnI GGCC 1 cut(s) 251
BsoBI CYCGRG 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 441
Bsp143I GATC 3 cut(s) 114, 354, 532
BspACI CCGC 5 cut(s) 83, 336, 422, 435, 536
BspANI GGCC 1 cut(s) 251
BspCNI CTCAG 3 cut(s) 102, 373, 460
BspMAI CTGCAG 1 cut(s) 282
BspPI GGATC 2 cut(s) 122, 527
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 2 cut(s) 157, 332
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 3 cut(s) 114, 354, 532
BssNI GRCGYC 1 cut(s) 360
Bst2UI CCWGG 3 cut(s) 72, 373, 540
Bst4CI ACNGT 2 cut(s) 49, 243
Bst6I CTCTTC 1 cut(s) 455
BstACI GRCGYC 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 253, 275, 437
BstDEI CTNAG 3 cut(s) 110, 381, 447
BstF5I GGATG 3 cut(s) 79, 105, 255
BstKTI GATC 3 cut(s) 117, 357, 535
BstMBI GATC 3 cut(s) 114, 354, 532
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 1 cut(s) 138
BstNI CCWGG 3 cut(s) 72, 373, 540
BstSCI CCNGG 4 cut(s) 70, 291, 371, 538
BstSFI CTRYAG 1 cut(s) 278
BstSLI GKGCMC 1 cut(s) 441
BstV1I GCAGC 6 cut(s) 125, 200, 264, 292, 345, 364
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 1 cut(s) 114
BstXI CCANNNNNNTGG 1 cut(s) 213
BstYI RGATCY 1 cut(s) 114
Bsu36I CCTNAGG 1 cut(s) 110
BsuRI GGCC 1 cut(s) 251
BtrI CACGTC 1 cut(s) 345
BtsCI GGATG 3 cut(s) 79, 105, 255
BtsIMutI CAGTG 1 cut(s) 45
Cac8I GCNNGC 3 cut(s) 253, 275, 437
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 1 cut(s) 249
CseI GACGC 1 cut(s) 368
Csp6I GTAC 3 cut(s) 235, 391, 465
CviAII CATG 2 cut(s) 178, 211
CviQI GTAC 3 cut(s) 235, 391, 465
DdeI CTNAG 3 cut(s) 110, 381, 447
DpnI GATC 3 cut(s) 116, 356, 534
DpnII GATC 3 cut(s) 114, 354, 532
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
EciI GGCGGA 1 cut(s) 525
Eco81I CCTNAGG 1 cut(s) 110
Eco88I CYCGRG 1 cut(s) 227
EcoRII CCWGG 3 cut(s) 70, 371, 538
FaeI CATG 2 cut(s) 181, 214
FaiI YATR 8 cut(s) 130, 179, 212, 402, 444, 518, 531, 549
FatI CATG 2 cut(s) 177, 210
FauI CCCGC 2 cut(s) 415, 428
Fnu4HI GCNGC 7 cut(s) 139, 189, 278, 281, 334, 337, 378
FokI GGATG 3 cut(s) 66, 92, 242
Fsp4HI GCNGC 7 cut(s) 139, 189, 278, 281, 334, 337, 378
FspBI CTAG 1 cut(s) 486
GluI GCNGC 7 cut(s) 139, 189, 278, 281, 334, 337, 378
GsaI CCCAGC 2 cut(s) 136, 314
GsuI CTGGAG 2 cut(s) 93, 140
HaeIII GGCC 1 cut(s) 251
HapII CCGG 2 cut(s) 252, 293
HgaI GACGC 1 cut(s) 368
Hin1I GRCGYC 1 cut(s) 360
Hin1II CATG 2 cut(s) 181, 214
HinfI GANTC 3 cut(s) 149, 349, 474
HpaII CCGG 2 cut(s) 252, 293
HphI GGTGA 2 cut(s) 60, 566
Hpy166II GTNNAC 1 cut(s) 348
Hpy188I TCNGA 3 cut(s) 260, 354, 450
Hpy188III TCNNGA 2 cut(s) 109, 229
Hpy8I GTNNAC 1 cut(s) 348
Hpy99I CGWCG 2 cut(s) 346, 362
HpyCH4III ACNGT 2 cut(s) 49, 243
HpyCH4IV ACGT 2 cut(s) 344, 413
HpyCH4V TGCA 5 cut(s) 45, 141, 273, 280, 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 138
HpyF3I CTNAG 3 cut(s) 110, 381, 447
HpySE526I ACGT 2 cut(s) 344, 413
Hsp92I GRCGYC 1 cut(s) 360
Hsp92II CATG 2 cut(s) 181, 214
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
Kzo9I GATC 3 cut(s) 114, 354, 532
Lsp1109I GCAGC 6 cut(s) 125, 200, 264, 292, 345, 364
LweI GCATC 2 cut(s) 192, 264
MaeI CTAG 1 cut(s) 486
MaeII ACGT 2 cut(s) 344, 413
MaeIII GTNAC 2 cut(s) 150, 524
MalI GATC 3 cut(s) 116, 356, 534
MboI GATC 3 cut(s) 114, 354, 532
MboII GAAGA 2 cut(s) 308, 472
MflI RGATCY 1 cut(s) 114
MhlI GDGCHC 1 cut(s) 441
MluCI AATT 2 cut(s) 27, 544
MlyI GAGTC 3 cut(s) 158, 343, 483
MmeI TCCRAC 1 cut(s) 193
MnlI CCTC 6 cut(s) 68, 105, 225, 376, 412, 462
MroNI GCCGGC 1 cut(s) 251
MseI TTAA 1 cut(s) 568
MslI CAYNNNNRTG 2 cut(s) 211, 244
MspA1I CMGCKG 1 cut(s) 336
MspI CCGG 2 cut(s) 252, 293
MspR9I CCNGG 4 cut(s) 72, 293, 373, 540
Mva1269I GAATGC 1 cut(s) 283
MvaI CCWGG 3 cut(s) 72, 373, 540
MwoI GCNNNNNNNGC 1 cut(s) 138
NaeI GCCGGC 1 cut(s) 253
NciI CCSGG 1 cut(s) 293
NdeII GATC 3 cut(s) 114, 354, 532
NgoMIV GCCGGC 1 cut(s) 251
NlaIII CATG 2 cut(s) 181, 214
NmuCI GTSAC 1 cut(s) 150
OliI CACNNNNGTG 2 cut(s) 211, 244
PaeR7I CTCGAG 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 350
PctI GAATGC 1 cut(s) 283
PdiI GCCGGC 1 cut(s) 253
PfoI TCCNGGA 1 cut(s) 291
PkrI GCNGC 7 cut(s) 140, 190, 279, 282, 335, 338, 379
Ple19I CGATCG 1 cut(s) 357
PleI GAGTC 3 cut(s) 157, 343, 482
PpsI GAGTC 3 cut(s) 157, 343, 482
Ppu21I YACGTR 1 cut(s) 414
Psp6I CCWGG 3 cut(s) 70, 371, 538
PspFI CCCAGC 2 cut(s) 132, 310
PspGI CCWGG 3 cut(s) 70, 371, 538
PspPI GGNCC 1 cut(s) 249
PstI CTGCAG 1 cut(s) 282
PsuI RGATCY 1 cut(s) 114
PvuI CGATCG 1 cut(s) 357
RsaI GTAC 3 cut(s) 236, 392, 466
RsaNI GTAC 3 cut(s) 235, 391, 465
RseI CAYNNNNRTG 2 cut(s) 211, 244
SaqAI TTAA 1 cut(s) 568
SatI GCNGC 7 cut(s) 139, 189, 278, 281, 334, 337, 378
Sau3AI GATC 3 cut(s) 114, 354, 532
Sau96I GGNCC 1 cut(s) 249
ScaI AGTACT 1 cut(s) 392
SchI GAGTC 3 cut(s) 158, 343, 483
ScrFI CCNGG 4 cut(s) 72, 293, 373, 540
SduI GDGCHC 1 cut(s) 441
SetI ASST 9 cut(s) 73, 79, 228, 236, 279, 312, 347, 374, 416
SfaNI GCATC 2 cut(s) 192, 264
SfcI CTRYAG 1 cut(s) 278
Sfr274I CTCGAG 1 cut(s) 227
SlaI CTCGAG 1 cut(s) 227
SmiMI CAYNNNNRTG 2 cut(s) 211, 244
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 2 cut(s) 27, 544
SsiI CCGC 5 cut(s) 83, 336, 422, 435, 536
SspI AATATT 1 cut(s) 322
SspMI CTAG 1 cut(s) 486
StyD4I CCNGG 4 cut(s) 70, 291, 371, 538
TaaI ACNGT 2 cut(s) 49, 243
TaiI ACGT 2 cut(s) 347, 416
TaqI TCGA 3 cut(s) 228, 341, 357
TasI AATT 2 cut(s) 27, 544
TatI WGTACW 1 cut(s) 390
TauI GCSGC 1 cut(s) 339
Tru1I TTAA 1 cut(s) 568
Tru9I TTAA 1 cut(s) 568
TscAI CASTG 1 cut(s) 52
TseFI GTSAC 1 cut(s) 150
TseI GCWGC 6 cut(s) 138, 188, 277, 280, 333, 377
Tsp45I GTSAC 1 cut(s) 150
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 52
XhoI CTCGAG 1 cut(s) 227
XspI CTAG 1 cut(s) 486
ZrmI AGTACT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.