RLG00000008971

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0017047
Physical Location & Seq
Forward (+)
44070697 .. 44073798
3102 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008971

Sequence Viewer

Length: 921 bp
ATGACGAACACCATCCTCTGTCGTCAAACCCTTCACCTCCGCCCAAGCTCGTCGAGACAACTCGGATCTTTTACCCCGTCGAATGCAACTCCAATCGAAAGGGGGTTATTGAGGAGCCTTGGGCTTTTCGGCCTCCGAGGAAGATTGCAAGGTCAGCAAATGACTGAAATTCTCTCGTCGTTCTCAGAAATATGTTGCGCATGCCTACCATGCCGGAATCGCATCCTTCGCCGGATCGAGTTGGTTGGAGGAAGGCAGGGGCATTCCAGTGCAAAAGGGAAGAGGATATGGAAAAGCTATGAAGTTAAAGATGAGAGCTTTAAGGAGAAAGATGGAGACTTTGGAATTCTAGAACGCACTCAATTTCTCTTTGGAAAGCTCAACTTCAGAGCCGAGTTTGGACTGGCATATTCTCTAACACCTGAAGCACATCCGCAAAAGCCCAACAACGAGACCATTCTGAGGATCTCCAAGCAACTATGCTGGGGCTGCATTGGAGAGTCACTCCAGTTCTTGTTCCAACACAACATGGTTAGGGCAGCAAAGTGGGAGATGCCACTCATGTGGGACTTCCAGCTCGAGAGGTACGCACGGTGGTGGGCCGGCATCCGAAAAGCCGACTGCAAGCTGCAGCATTCGTTCCCGGAAGACGGGTTCAAGCTGGGAGAGAACATATACTGGGGCAGCGGCTCGACGTGGACTCCGATCGACGCAGTGAATACCTGGGCCGCTGAGGAGAAGTACTACACTTACGCAACGAACACCTGTGAGGCGGGTCAAATGTGCGGGCACTATACTCAGATTGTGTGGAAAAGTACGAGGAGAGTCGGGTGTGCTAGGGTGGTGTGTGATGATGGAGATGTGTTTATGACTTGTAATTATGATCCGCCTGGTAATTATATTGGTGAGAAGCCCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP

Protein Analysis

307

Amino Acids

35.08

Weight (kDa)

8.83

Isoelectric Point (pI)

44.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 174 - 294 4.5e-19 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25780
fragaria_vesca FvH4_4g09940
malus_domestica MD13G1265800.v1.1
prunus_persica Prupe.1G091400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0403511
rosa_laevigata RLG00000008971
rosa_multiflora Rmu_sc0007727.1_g000036
rosa_roxburghii Rroxscaffold_5G00348180
rosa_rugosa Rorug04G0043900
rosa_samantha Rh4AG119300 Rh4CG127200 Rh4DG112000
rosa_wichuraiana Rw4G009680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 199
AciI CCGC 7 cut(s) 40, 434, 687, 729, 773, 786, 887
AclWI GGATC 4 cut(s) 73, 242, 473, 878
AcsI RAATTY 2 cut(s) 168, 345
AcuI CTGAAG 2 cut(s) 370, 444
AfaI GTAC 3 cut(s) 587, 743, 817
AfiI CCNNNNNNNGG 2 cut(s) 462, 650
AgsI TTSAA 1 cut(s) 658
AjiI CACGTC 1 cut(s) 696
AjnI CCWGG 2 cut(s) 722, 889
AleI CACNNNNGTG 2 cut(s) 562, 595
AloI GAACNNNNNNTCC 2 cut(s) 638, 670
AluBI AGCT 7 cut(s) 48, 297, 318, 379, 577, 628, 661
AluI AGCT 7 cut(s) 48, 297, 318, 379, 577, 628, 661
Alw26I GTCTC 3 cut(s) 49, 330, 446
AlwI GGATC 4 cut(s) 73, 242, 473, 878
Ama87I CYCGRG 1 cut(s) 578
AoxI GGCC 3 cut(s) 130, 600, 726
ApeKI GCWGC 5 cut(s) 489, 539, 628, 631, 684
ApoI RAATTY 2 cut(s) 168, 345
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 2 cut(s) 600, 726
AsuC2I CCSGG 1 cut(s) 644
AsuHPI GGTGA 2 cut(s) 26, 917
AvaI CYCGRG 1 cut(s) 578
BaeGI GKGCMC 1 cut(s) 792
BbsI GAAGAC 1 cut(s) 654
BbvCI CCTCAGC 1 cut(s) 732
BbvI GCAGC 5 cut(s) 476, 551, 615, 643, 696
BccI CCATC 3 cut(s) 20, 326, 848
BciT130I CCWGG 2 cut(s) 724, 891
BcnI CCSGG 1 cut(s) 644
BcoDI GTCTC 3 cut(s) 49, 330, 446
BfaI CTAG 2 cut(s) 350, 837
BfmI CTRYAG 1 cut(s) 629
BisI GCNGC 7 cut(s) 490, 540, 629, 632, 685, 688, 729
BlsI GCNGC 7 cut(s) 491, 541, 630, 633, 686, 689, 730
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 3 cut(s) 644, 724, 891
BmeT110I CYCGRG 1 cut(s) 578
BmgBI CACGTC 1 cut(s) 696
BmgT120I GGNCC 2 cut(s) 600, 726
BmiI GGNNCC 1 cut(s) 116
BmrFI CCNGG 3 cut(s) 644, 724, 891
BmrI ACTGGG 1 cut(s) 688
BmsI GCATC 3 cut(s) 231, 543, 615
BmuI ACTGGG 1 cut(s) 688
BpiI GAAGAC 1 cut(s) 654
BplI GAGNNNNNCTC 2 cut(s) 489, 521
BpmI CTGGAG 1 cut(s) 491
Bpu10I CCTNAGC 1 cut(s) 732
BpuMI CCSGG 1 cut(s) 644
BsaI GGTCTC 1 cut(s) 446
BsaJI CCNNGG 3 cut(s) 118, 136, 723
BsaXI ACNNNNNCTCC 2 cut(s) 685, 715
Bsc4I CCNNNNNNNGG 2 cut(s) 462, 650
Bse118I RCCGGY 1 cut(s) 602
Bse1I ACTGG 4 cut(s) 267, 408, 508, 683
BseBI CCWGG 2 cut(s) 724, 891
BseDI CCNNGG 3 cut(s) 118, 136, 723
BseGI GGATG 4 cut(s) 12, 222, 430, 606
BseLI CCNNNNNNNGG 2 cut(s) 462, 650
BseMII CTCAG 4 cut(s) 198, 452, 723, 812
BseNI ACTGG 4 cut(s) 267, 408, 508, 683
BseRI GAGGAG 3 cut(s) 127, 749, 835
BseSI GKGCMC 1 cut(s) 792
BseXI GCAGC 5 cut(s) 476, 551, 615, 643, 696
BseYI CCCAGC 2 cut(s) 483, 661
Bsh1285I CGRYCG 1 cut(s) 708
BshFI GGCC 3 cut(s) 132, 602, 728
BsiEI CGRYCG 1 cut(s) 708
BsiHKCI CYCGRG 1 cut(s) 578
BsiSI CCGG 4 cut(s) 214, 232, 603, 644
BslFI GGGAC 1 cut(s) 581
BslI CCNNNNNNNGG 2 cut(s) 462, 650
BsmAI GTCTC 3 cut(s) 49, 330, 446
BsmFI GGGAC 1 cut(s) 581
BsmI GAATGC 3 cut(s) 88, 262, 634
BsnI GGCC 3 cut(s) 132, 602, 728
Bso31I GGTCTC 1 cut(s) 446
BsoBI CYCGRG 1 cut(s) 578
Bsp1286I GDGCHC 1 cut(s) 792
Bsp143I GATC 5 cut(s) 65, 234, 465, 705, 883
BspACI CCGC 7 cut(s) 40, 434, 687, 729, 773, 786, 887
BspANI GGCC 3 cut(s) 132, 602, 728
BspCNI CTCAG 4 cut(s) 197, 453, 724, 811
BspLI GGNNCC 1 cut(s) 116
BspMAI CTGCAG 1 cut(s) 633
BspPI GGATC 4 cut(s) 73, 242, 473, 878
BspTNI GGTCTC 1 cut(s) 446
BsrFI RCCGGY 1 cut(s) 602
BsrI ACTGG 4 cut(s) 267, 408, 508, 683
BssAI RCCGGY 1 cut(s) 602
BssECI CCNNGG 3 cut(s) 118, 136, 723
BssMI GATC 5 cut(s) 65, 234, 465, 705, 883
BssT1I CCWWGG 1 cut(s) 118
Bst2UI CCWGG 2 cut(s) 724, 891
Bst4CI ACNGT 1 cut(s) 594
Bst6I CTCTTC 1 cut(s) 275
BstC8I GCNNGC 4 cut(s) 202, 604, 626, 788
BstDEI CTNAG 4 cut(s) 184, 461, 732, 798
BstF5I GGATG 4 cut(s) 12, 222, 430, 606
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 5 cut(s) 68, 237, 468, 708, 886
BstMAI GTCTC 3 cut(s) 49, 330, 446
BstMBI GATC 5 cut(s) 65, 234, 465, 705, 883
BstMCI CGRYCG 1 cut(s) 708
BstMWI GCNNNNNNNGC 5 cut(s) 154, 210, 219, 228, 489
BstNI CCWGG 2 cut(s) 724, 891
BstNSI RCATGY 1 cut(s) 204
BstSCI CCNGG 3 cut(s) 642, 722, 889
BstSFI CTRYAG 1 cut(s) 629
BstSLI GKGCMC 1 cut(s) 792
BstV1I GCAGC 5 cut(s) 476, 551, 615, 643, 696
BstV2I GAAGAC 1 cut(s) 654
BstX2I RGATCY 2 cut(s) 65, 465
BstXI CCANNNNNNTGG 1 cut(s) 564
BstYI RGATCY 2 cut(s) 65, 465
BsuRI GGCC 3 cut(s) 132, 602, 728
BtrI CACGTC 1 cut(s) 696
BtsCI GGATG 4 cut(s) 12, 222, 430, 606
BtsI GCAGTG 1 cut(s) 720
BtsIMutI CAGTG 2 cut(s) 274, 720
Cac8I GCNNGC 4 cut(s) 202, 604, 626, 788
CfoI GCGC 1 cut(s) 200
Cfr10I RCCGGY 1 cut(s) 602
Cfr13I GGNCC 2 cut(s) 600, 726
CseI GACGC 1 cut(s) 719
Csp6I GTAC 3 cut(s) 586, 742, 816
CviAII CATG 4 cut(s) 201, 210, 529, 562
CviQI GTAC 3 cut(s) 586, 742, 816
DdeI CTNAG 4 cut(s) 184, 461, 732, 798
DpnI GATC 5 cut(s) 67, 236, 467, 707, 885
DpnII GATC 5 cut(s) 65, 234, 465, 705, 883
Eam1104I CTCTTC 1 cut(s) 275
EarI CTCTTC 1 cut(s) 275
EciI GGCGGA 2 cut(s) 29, 876
Eco130I CCWWGG 1 cut(s) 118
Eco31I GGTCTC 1 cut(s) 446
Eco57I CTGAAG 2 cut(s) 370, 444
Eco88I CYCGRG 1 cut(s) 578
EcoRI GAATTC 1 cut(s) 345
EcoRII CCWGG 2 cut(s) 722, 889
EcoT14I CCWWGG 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 4 cut(s) 204, 213, 532, 565
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FaqI GGGAC 1 cut(s) 581
FatI CATG 4 cut(s) 200, 209, 528, 561
FauI CCCGC 2 cut(s) 766, 779
Fnu4HI GCNGC 7 cut(s) 490, 540, 629, 632, 685, 688, 729
FokI GGATG 3 cut(s) 209, 417, 593
Fsp4HI GCNGC 7 cut(s) 490, 540, 629, 632, 685, 688, 729
FspBI CTAG 2 cut(s) 350, 837
FspI TGCGCA 1 cut(s) 199
GlaI GCGC 1 cut(s) 199
GluI GCNGC 7 cut(s) 490, 540, 629, 632, 685, 688, 729
GsaI CCCAGC 2 cut(s) 487, 665
GsuI CTGGAG 1 cut(s) 491
HaeIII GGCC 3 cut(s) 132, 602, 728
HapII CCGG 4 cut(s) 214, 232, 603, 644
HgaI GACGC 1 cut(s) 719
HhaI GCGC 1 cut(s) 200
Hin1II CATG 4 cut(s) 204, 213, 532, 565
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HinfI GANTC 4 cut(s) 217, 500, 700, 825
HpaII CCGG 4 cut(s) 214, 232, 603, 644
HphI GGTGA 2 cut(s) 26, 917
Hpy166II GTNNAC 1 cut(s) 699
Hpy188I TCNGA 8 cut(s) 65, 137, 187, 389, 462, 611, 705, 801
Hpy188III TCNNGA 3 cut(s) 54, 350, 580
Hpy8I GTNNAC 1 cut(s) 699
Hpy99I CGWCG 5 cut(s) 55, 82, 181, 697, 713
HpyAV CCTTC 3 cut(s) 41, 236, 246
HpyCH4III ACNGT 1 cut(s) 594
HpyCH4IV ACGT 1 cut(s) 695
HpyCH4V TGCA 6 cut(s) 86, 148, 272, 492, 624, 631
HpyF10VI GCNNNNNNNGC 5 cut(s) 154, 210, 219, 228, 489
HpyF3I CTNAG 4 cut(s) 184, 461, 732, 798
HpySE526I ACGT 1 cut(s) 695
Hsp92II CATG 4 cut(s) 204, 213, 532, 565
HspAI GCGC 1 cut(s) 198
KroI GCCGGC 1 cut(s) 602
KroNI GCCGGC 1 cut(s) 604
Kzo9I GATC 5 cut(s) 65, 234, 465, 705, 883
LmnI GCTCC 1 cut(s) 114
Lsp1109I GCAGC 5 cut(s) 476, 551, 615, 643, 696
LweI GCATC 3 cut(s) 231, 543, 615
MaeI CTAG 2 cut(s) 350, 837
MaeII ACGT 1 cut(s) 695
MaeIII GTNAC 1 cut(s) 501
MalI GATC 5 cut(s) 67, 236, 467, 707, 885
MboI GATC 5 cut(s) 65, 234, 465, 705, 883
MboII GAAGA 3 cut(s) 153, 292, 659
MflI RGATCY 2 cut(s) 65, 465
MhlI GDGCHC 1 cut(s) 792
MluCI AATT 5 cut(s) 168, 345, 362, 877, 895
MlyI GAGTC 3 cut(s) 509, 694, 834
MmeI TCCRAC 2 cut(s) 226, 544
MroNI GCCGGC 1 cut(s) 602
MseI TTAA 3 cut(s) 306, 321, 919
MslI CAYNNNNRTG 3 cut(s) 267, 562, 595
MspA1I CMGCKG 2 cut(s) 687, 731
MspI CCGG 4 cut(s) 214, 232, 603, 644
MspR9I CCNGG 3 cut(s) 644, 724, 891
Mva1269I GAATGC 3 cut(s) 88, 262, 634
MvaI CCWGG 2 cut(s) 724, 891
MwoI GCNNNNNNNGC 5 cut(s) 154, 210, 219, 228, 489
NaeI GCCGGC 1 cut(s) 604
NciI CCSGG 1 cut(s) 644
NdeII GATC 5 cut(s) 65, 234, 465, 705, 883
NgoMIV GCCGGC 1 cut(s) 602
NlaIII CATG 4 cut(s) 204, 213, 532, 565
NlaIV GGNNCC 1 cut(s) 116
NmeAIII GCCGAG 1 cut(s) 418
NmuCI GTSAC 1 cut(s) 501
NsbI TGCGCA 1 cut(s) 199
NspI RCATGY 1 cut(s) 204
OliI CACNNNNGTG 2 cut(s) 562, 595
PaeI GCATGC 1 cut(s) 204
PaeR7I CTCGAG 1 cut(s) 578
PcsI WCGNNNNNNNCGW 1 cut(s) 701
PctI GAATGC 3 cut(s) 88, 262, 634
PdiI GCCGGC 1 cut(s) 604
PfeI GAWTC 1 cut(s) 217
PfoI TCCNGGA 1 cut(s) 642
PkrI GCNGC 7 cut(s) 491, 541, 630, 633, 686, 689, 730
Ple19I CGATCG 1 cut(s) 708
PleI GAGTC 3 cut(s) 508, 694, 833
PpsI GAGTC 3 cut(s) 508, 694, 833
Psp6I CCWGG 2 cut(s) 722, 889
PspFI CCCAGC 2 cut(s) 483, 661
PspGI CCWGG 2 cut(s) 722, 889
PspN4I GGNNCC 1 cut(s) 116
PspPI GGNCC 2 cut(s) 600, 726
PstI CTGCAG 1 cut(s) 633
PsuI RGATCY 2 cut(s) 65, 465
PvuI CGATCG 1 cut(s) 708
RsaI GTAC 3 cut(s) 587, 743, 817
RsaNI GTAC 3 cut(s) 586, 742, 816
RseI CAYNNNNRTG 3 cut(s) 267, 562, 595
SaqAI TTAA 3 cut(s) 306, 321, 919
SatI GCNGC 7 cut(s) 490, 540, 629, 632, 685, 688, 729
Sau3AI GATC 5 cut(s) 65, 234, 465, 705, 883
Sau96I GGNCC 2 cut(s) 600, 726
ScaI AGTACT 1 cut(s) 743
SchI GAGTC 3 cut(s) 509, 694, 834
ScrFI CCNGG 3 cut(s) 644, 724, 891
SduI GDGCHC 1 cut(s) 792
SfaNI GCATC 3 cut(s) 231, 543, 615
SfcI CTRYAG 1 cut(s) 629
Sfr274I CTCGAG 1 cut(s) 578
SlaI CTCGAG 1 cut(s) 578
SmiMI CAYNNNNRTG 3 cut(s) 267, 562, 595
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
SphI GCATGC 1 cut(s) 204
Sse9I AATT 5 cut(s) 168, 345, 362, 877, 895
SsiI CCGC 7 cut(s) 40, 434, 687, 729, 773, 786, 887
SspMI CTAG 2 cut(s) 350, 837
StyD4I CCNGG 3 cut(s) 642, 722, 889
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 1 cut(s) 594
TaiI ACGT 1 cut(s) 698
TaqI TCGA 7 cut(s) 53, 80, 96, 237, 579, 692, 708
TasI AATT 5 cut(s) 168, 345, 362, 877, 895
TatI WGTACW 1 cut(s) 741
TauI GCSGC 2 cut(s) 690, 731
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 3 cut(s) 306, 321, 919
Tru9I TTAA 3 cut(s) 306, 321, 919
TscAI CASTG 2 cut(s) 274, 720
TseFI GTSAC 1 cut(s) 501
TseI GCWGC 5 cut(s) 489, 539, 628, 631, 684
Tsp45I GTSAC 1 cut(s) 501
TspDTI ATGAA 1 cut(s) 315
TspRI CASTG 2 cut(s) 274, 720
XapI RAATTY 2 cut(s) 168, 345
XbaI TCTAGA 1 cut(s) 349
XceI RCATGY 1 cut(s) 204
XhoI CTCGAG 1 cut(s) 578
XspI CTAG 2 cut(s) 350, 837
ZrmI AGTACT 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.