Rh4DG112000

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0017047
Physical Location & Seq
Reverse (-)
17531469 .. 17534095
2627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG112000.1

Sequence Viewer

Length: 564 bp
ATGAAATCACGTTTGCCCTTAATTCTCTTTGCTCTCTGCACTTCTCAGGCATATTCTCTAACACCTGAAGCACATCCGCAAAAGCCCAACAACGAGACCATTCTGAGGATCTCCAAGCAACTATGCTGGGGCTGCATTGGAGAGTCACTCCAGTTCTTGTTCCAACACAACATGGTTAGGGCAGCAAAGTGGGAGATGCCACTCATGTGGGACTTCCAGCTCGAGAGGTACGCACGGTGGTGGGCCGGCATCCGAAAAGCCGACTGCAAGCTGCAGCATTCGTTCCCGGAAGACGGGTTCAAGCTGGGAGAGAACATATACTGGGGCAGCGGCTCGACGTGGACTCCGATCGACGCAGTGAATACCTGGGCCGCTGAGGAGAAGTACTACACTTACGCAACGAACACCTGTGAGGCGGGTCAAATGTGCGGGCACTATACTCAGATTGTGTGGAAAAGTACGAGGAGAGTCGGGTGTGCTAGGGTGGTGTGTGATGATGGAGATGTGTTTATGACTTGTAATTATGATCCGCCCGGTAATTATATTGGTGAGAAGCCCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP

Protein Analysis

187

Amino Acids

21.6

Weight (kDa)

6.87

Isoelectric Point (pI)

35.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 55 - 175 1.3e-19 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017047)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25780
fragaria_vesca FvH4_4g09940
malus_domestica MD13G1265800.v1.1
prunus_persica Prupe.1G091400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0403511
rosa_laevigata RLG00000008971
rosa_multiflora Rmu_sc0007727.1_g000036
rosa_roxburghii Rroxscaffold_5G00348180
rosa_rugosa Rorug04G0043900
rosa_samantha Rh4AG119300 Rh4CG127200 Rh4DG112000
rosa_wichuraiana Rw4G009680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 77, 330, 372, 416, 429, 530
AclWI GGATC 2 cut(s) 116, 521
AcuI CTGAAG 1 cut(s) 87
AfaI GTAC 3 cut(s) 230, 386, 460
AfiI CCNNNNNNNGG 2 cut(s) 105, 293
AgsI TTSAA 1 cut(s) 301
AjiI CACGTC 1 cut(s) 339
AjnI CCWGG 1 cut(s) 365
AleI CACNNNNGTG 2 cut(s) 205, 238
AloI GAACNNNNNNTCC 2 cut(s) 281, 313
AluBI AGCT 3 cut(s) 220, 271, 304
AluI AGCT 3 cut(s) 220, 271, 304
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 2 cut(s) 116, 521
Ama87I CYCGRG 1 cut(s) 221
AoxI GGCC 2 cut(s) 243, 369
ApeKI GCWGC 5 cut(s) 132, 182, 271, 274, 327
AspS9I GGNCC 2 cut(s) 243, 369
AsuC2I CCSGG 2 cut(s) 287, 534
AsuHPI GGTGA 1 cut(s) 560
AvaI CYCGRG 1 cut(s) 221
BaeGI GKGCMC 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 297
BbvCI CCTCAGC 1 cut(s) 375
BbvI GCAGC 5 cut(s) 119, 194, 258, 286, 339
BccI CCATC 1 cut(s) 491
BciT130I CCWGG 1 cut(s) 367
BcnI CCSGG 2 cut(s) 287, 534
BcoDI GTCTC 1 cut(s) 89
BfaI CTAG 1 cut(s) 480
BfmI CTRYAG 1 cut(s) 272
BisI GCNGC 7 cut(s) 133, 183, 272, 275, 328, 331, 372
BlsI GCNGC 7 cut(s) 134, 184, 273, 276, 329, 332, 373
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 3 cut(s) 287, 367, 534
BmeT110I CYCGRG 1 cut(s) 221
BmgBI CACGTC 1 cut(s) 339
BmgT120I GGNCC 2 cut(s) 243, 369
BmrFI CCNGG 3 cut(s) 287, 367, 534
BmrI ACTGGG 1 cut(s) 331
BmsI GCATC 2 cut(s) 186, 258
BmuI ACTGGG 1 cut(s) 331
BpiI GAAGAC 1 cut(s) 297
BplI GAGNNNNNCTC 2 cut(s) 132, 164
BpmI CTGGAG 1 cut(s) 134
Bpu10I CCTNAGC 1 cut(s) 375
BpuMI CCSGG 2 cut(s) 287, 534
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 1 cut(s) 366
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 293
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 2 cut(s) 151, 326
BseBI CCWGG 1 cut(s) 367
BseDI CCNNGG 1 cut(s) 366
BseGI GGATG 2 cut(s) 73, 249
BseLI CCNNNNNNNGG 2 cut(s) 105, 293
BseMII CTCAG 4 cut(s) 59, 95, 366, 455
BseNI ACTGG 2 cut(s) 151, 326
BseRI GAGGAG 2 cut(s) 392, 478
BseSI GKGCMC 1 cut(s) 435
BseXI GCAGC 5 cut(s) 119, 194, 258, 286, 339
BseYI CCCAGC 2 cut(s) 126, 304
BsgI GTGCAG 1 cut(s) 22
Bsh1285I CGRYCG 1 cut(s) 351
BshFI GGCC 2 cut(s) 245, 371
BsiEI CGRYCG 1 cut(s) 351
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 3 cut(s) 246, 287, 534
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 2 cut(s) 105, 293
BsmAI GTCTC 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 224
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 2 cut(s) 245, 371
Bso31I GGTCTC 1 cut(s) 89
BsoBI CYCGRG 1 cut(s) 221
Bsp1286I GDGCHC 1 cut(s) 435
Bsp143I GATC 3 cut(s) 108, 348, 526
BspACI CCGC 6 cut(s) 77, 330, 372, 416, 429, 530
BspANI GGCC 2 cut(s) 245, 371
BspCNI CTCAG 4 cut(s) 58, 96, 367, 454
BspMAI CTGCAG 1 cut(s) 276
BspPI GGATC 2 cut(s) 116, 521
BspTNI GGTCTC 1 cut(s) 89
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 2 cut(s) 151, 326
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 1 cut(s) 366
BssMI GATC 3 cut(s) 108, 348, 526
Bst2UI CCWGG 1 cut(s) 367
Bst4CI ACNGT 1 cut(s) 237
BstC8I GCNNGC 3 cut(s) 247, 269, 431
BstDEI CTNAG 4 cut(s) 45, 104, 375, 441
BstF5I GGATG 2 cut(s) 73, 249
BstKTI GATC 3 cut(s) 111, 351, 529
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 3 cut(s) 108, 348, 526
BstMCI CGRYCG 1 cut(s) 351
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstNI CCWGG 1 cut(s) 367
BstSCI CCNGG 3 cut(s) 285, 365, 532
BstSFI CTRYAG 1 cut(s) 272
BstSLI GKGCMC 1 cut(s) 435
BstV1I GCAGC 5 cut(s) 119, 194, 258, 286, 339
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 1 cut(s) 108
BstXI CCANNNNNNTGG 1 cut(s) 207
BstYI RGATCY 1 cut(s) 108
BsuRI GGCC 2 cut(s) 245, 371
BtrI CACGTC 1 cut(s) 339
BtsCI GGATG 2 cut(s) 73, 249
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 1 cut(s) 363
Cac8I GCNNGC 3 cut(s) 247, 269, 431
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 2 cut(s) 243, 369
CseI GACGC 1 cut(s) 362
Csp6I GTAC 3 cut(s) 229, 385, 459
CviAII CATG 2 cut(s) 172, 205
CviQI GTAC 3 cut(s) 229, 385, 459
DdeI CTNAG 4 cut(s) 45, 104, 375, 441
DpnI GATC 3 cut(s) 110, 350, 528
DpnII GATC 3 cut(s) 108, 348, 526
EciI GGCGGA 1 cut(s) 519
Eco31I GGTCTC 1 cut(s) 89
Eco57I CTGAAG 1 cut(s) 87
Eco88I CYCGRG 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 365
FaeI CATG 2 cut(s) 175, 208
FaqI GGGAC 1 cut(s) 224
FatI CATG 2 cut(s) 171, 204
FauI CCCGC 2 cut(s) 409, 422
Fnu4HI GCNGC 7 cut(s) 133, 183, 272, 275, 328, 331, 372
FokI GGATG 2 cut(s) 60, 236
Fsp4HI GCNGC 7 cut(s) 133, 183, 272, 275, 328, 331, 372
FspBI CTAG 1 cut(s) 480
GluI GCNGC 7 cut(s) 133, 183, 272, 275, 328, 331, 372
GsaI CCCAGC 2 cut(s) 130, 308
GsuI CTGGAG 1 cut(s) 134
HaeIII GGCC 2 cut(s) 245, 371
HapII CCGG 3 cut(s) 246, 287, 534
HgaI GACGC 1 cut(s) 362
Hin1II CATG 2 cut(s) 175, 208
HinfI GANTC 3 cut(s) 143, 343, 468
HpaII CCGG 3 cut(s) 246, 287, 534
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 1 cut(s) 342
Hpy188I TCNGA 4 cut(s) 105, 254, 348, 444
Hpy188III TCNNGA 1 cut(s) 223
Hpy8I GTNNAC 1 cut(s) 342
Hpy99I CGWCG 2 cut(s) 340, 356
HpyCH4III ACNGT 1 cut(s) 237
HpyCH4IV ACGT 2 cut(s) 10, 338
HpyCH4V TGCA 4 cut(s) 39, 135, 267, 274
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 4 cut(s) 45, 104, 375, 441
HpySE526I ACGT 2 cut(s) 10, 338
Hsp92II CATG 2 cut(s) 175, 208
KroI GCCGGC 1 cut(s) 245
KroNI GCCGGC 1 cut(s) 247
Kzo9I GATC 3 cut(s) 108, 348, 526
Lsp1109I GCAGC 5 cut(s) 119, 194, 258, 286, 339
LweI GCATC 2 cut(s) 186, 258
MaeI CTAG 1 cut(s) 480
MaeII ACGT 2 cut(s) 10, 338
MaeIII GTNAC 1 cut(s) 144
MalI GATC 3 cut(s) 110, 350, 528
MboI GATC 3 cut(s) 108, 348, 526
MboII GAAGA 1 cut(s) 302
MflI RGATCY 1 cut(s) 108
MhlI GDGCHC 1 cut(s) 435
MluCI AATT 3 cut(s) 21, 520, 538
MlyI GAGTC 3 cut(s) 152, 337, 477
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 5 cut(s) 99, 219, 370, 406, 456
MroNI GCCGGC 1 cut(s) 245
MseI TTAA 2 cut(s) 20, 562
MslI CAYNNNNRTG 2 cut(s) 205, 238
MspA1I CMGCKG 2 cut(s) 330, 374
MspI CCGG 3 cut(s) 246, 287, 534
MspR9I CCNGG 3 cut(s) 287, 367, 534
Mva1269I GAATGC 1 cut(s) 277
MvaI CCWGG 1 cut(s) 367
MwoI GCNNNNNNNGC 1 cut(s) 132
NaeI GCCGGC 1 cut(s) 247
NciI CCSGG 2 cut(s) 287, 534
NdeII GATC 3 cut(s) 108, 348, 526
NgoMIV GCCGGC 1 cut(s) 245
NlaIII CATG 2 cut(s) 175, 208
NmuCI GTSAC 1 cut(s) 144
OliI CACNNNNGTG 2 cut(s) 205, 238
PaeR7I CTCGAG 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 344
PctI GAATGC 1 cut(s) 277
PdiI GCCGGC 1 cut(s) 247
PfoI TCCNGGA 1 cut(s) 285
PkrI GCNGC 7 cut(s) 134, 184, 273, 276, 329, 332, 373
Ple19I CGATCG 1 cut(s) 351
PleI GAGTC 3 cut(s) 151, 337, 476
PpsI GAGTC 3 cut(s) 151, 337, 476
Psp6I CCWGG 1 cut(s) 365
PspFI CCCAGC 2 cut(s) 126, 304
PspGI CCWGG 1 cut(s) 365
PspPI GGNCC 2 cut(s) 243, 369
PstI CTGCAG 1 cut(s) 276
PsuI RGATCY 1 cut(s) 108
PvuI CGATCG 1 cut(s) 351
RsaI GTAC 3 cut(s) 230, 386, 460
RsaNI GTAC 3 cut(s) 229, 385, 459
RseI CAYNNNNRTG 2 cut(s) 205, 238
SaqAI TTAA 2 cut(s) 20, 562
SatI GCNGC 7 cut(s) 133, 183, 272, 275, 328, 331, 372
Sau3AI GATC 3 cut(s) 108, 348, 526
Sau96I GGNCC 2 cut(s) 243, 369
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 3 cut(s) 152, 337, 477
ScrFI CCNGG 3 cut(s) 287, 367, 534
SduI GDGCHC 1 cut(s) 435
SetI ASST 9 cut(s) 13, 67, 222, 230, 273, 306, 341, 368, 410
SfaNI GCATC 2 cut(s) 186, 258
SfcI CTRYAG 1 cut(s) 272
Sfr274I CTCGAG 1 cut(s) 221
SlaI CTCGAG 1 cut(s) 221
SmiMI CAYNNNNRTG 2 cut(s) 205, 238
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 3 cut(s) 21, 520, 538
SsiI CCGC 6 cut(s) 77, 330, 372, 416, 429, 530
SspMI CTAG 1 cut(s) 480
StyD4I CCNGG 3 cut(s) 285, 365, 532
TaaI ACNGT 1 cut(s) 237
TaiI ACGT 2 cut(s) 13, 341
TaqI TCGA 3 cut(s) 222, 335, 351
TasI AATT 3 cut(s) 21, 520, 538
TatI WGTACW 1 cut(s) 384
TauI GCSGC 2 cut(s) 333, 374
Tru1I TTAA 2 cut(s) 20, 562
Tru9I TTAA 2 cut(s) 20, 562
TscAI CASTG 1 cut(s) 363
TseFI GTSAC 1 cut(s) 144
TseI GCWGC 5 cut(s) 132, 182, 271, 274, 327
Tsp45I GTSAC 1 cut(s) 144
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 363
XhoI CTCGAG 1 cut(s) 221
XspI CTAG 1 cut(s) 480
ZrmI AGTACT 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.