Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006514

Physical Location & Seq

Genomic Coordinates Reverse (-)

13614822 .. 13619312

Gene Structure 4491 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G27180.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGGTTGGGGAAATGACGAACAATGGAAGAATCCGGCCATCTTTTCCGGTGAAAGATCTGACCAGTAATGAAGGATCTGAATATGGTGGCCCTGTTGAGTTTACCAGAGAAGATGTTGAAACTCTTCTTCATGAGCGAATCAAGTACAAGAGCAAGTACAATTACAAGGAGAGATGTGAGAATACGATGGACTATGTAAAAAGGCTTAGGCTTTGCATTAGATGGTTTCAAGAACTCGAGTTAGATTATGCATTTGAGCAAGAGAAGTTGAAGAACGCTATGGAGATGAATGAGAAGCATTGCGCCGATTTGGAGGTTAATTTGAAAGTAAAGGAAGAGGAGCTAAATATGGTAATTGATGAACTGAGGAAGAACTTTGCATCTGTTCAGGTGCAACTTGCCAAAGAACAAACAGAGAAATTGGCTGCAAATGAATCTCTTGGAAAAGAGAGAGAAGCAAGAATTGCTGTTGAAAGTTTGCAAGCTGCCATTACAGAAGAGCTAGCAAAGACGCAAGGAGAGCTTCAAACAGCTAACCAGAGGATTCAAGCTGTCAATGACATGTACAAACTCTTGCAAGAGTATAACTCGAGCTTACAGTTGTACAATAGCAAACTTCAAGGTGATCTCGACGAAGCTCATGAAAATATAAAGCGAGGAGAGAAAGAACGGACTGGCATTGTAGAAAGCATTGGCAACTTAAAGGGTCAGTTTAAAGCACTACAGGATCAGCTTGCTGCCTCGAAGGTGTCACAAGATGATGTCATGAAGCAAAAAGATGAATTGGTGAATGAAATTGTAAGTCTCAAGGTTGAGATTCAACAGGTCAAAGATGACCGTGACCGCCATATAACGGAAATTGAAACTCTGCAAGCAGAAGCAACCAAGCAAAATGACTTCAAAGACACCATAAATGAGCTTGAGAGTAAGTGTTCTGTTCAAAATAAGGAGATAGAAGAGTTGCAGGATCAATTAGTAGCTTCTGAGAGAAAACTGCAGGTGGCTGATTTATCTACTTTTGAGAAAATGAACGAGTTTGAAGAGCAAAAGGAAAGCATTATGGAGCTGAAAGGTCGCTTAGAAGAAGCGGAACTTAAACTCATCGAAGGAGAAAAACTACGGAAGAAGTTGCACAATACCATACAGGAACTGAAGGGAAACATCCGTGTATTCTGTAGGGTGAGACCTCTATTATCAGGAGAGAATTCTAGTGAGGAGGCCAAAACTATATCCTACCCTACATCTCTAGAAGCACTTGGTCGGGGCATTGACTTGTTGCAAAATGGACAATCGCATTGTTTCACATTTGATAAGGTTTTTGTGCCAAGTGCATCACAAGAAGATGTTTTTGTAGAGATTTCTCAACTTGTCCAGAGCGCTCTTGATGGTTACAAGGTTTGCATTTTCGCATATGGACAAACAGGATCTGGAAAAACTTATACAATGATGGGTAGGCCAGGAAACCCGGATGAAAAAGGACTGATTCCTCGATGTTTGGAGCAAATATTTCAAACAAGGCAGTCTCTTCGATCACAGGGTTGGAAGTATGAGTTGCAGGTGTCTATGTTAGAAATATACAACGAAACGATTAGGGATCTCTTGTCAACAAACAAGGAAGCTGTGAGAGCAGACAATGGCGTTTCTCCGCAGAAATATGCAATCAAACATGATGCTAGTGGGAATACACATGTTGTGGAACTTACTGTTGTAGATGTTCGAAGCTCGAAACAGGTTTCGTTCCTCTTAGACCACGCAGCTCGAAACAGGTCAGTAGGGAAGACCGCAATGAACGAGCAATCTTCTAGAAGCCATTTCGTTTTCACATTGAAGATCTCTGGATTCAACGAGAGCACAGAGCAACAAGTACAAGGTGTCTTGAACCTGATTGATCTTGCGGGTAGTGAGCGTTTGTCAAAGAGCGGATCAACTGGCGATAGACTGAAAGAAACTCAAGCAATCAACAAAAGCTTATCGTCTCTAGGCGATGTCATATTTGCCTTAGCGAAGAAAGAAGATCACGTTCCTTTCAGAAACTCAAAGCTCACTTATCTTCTTCAGCCCTGTTTAGGTGGTGACTCGAAGACGCTAATGTTTGTGAACATCACACCAGAACCTTCCTCAACCGGTGAGTCTCTGTGCTCCCTCAGGTTTGCAGCAAGAGTGAATGCTTGTGAGATTGGAACTGCACACCGTCATGTTAACGCCAGGCCGTTAGATTATCGCTTGAGCCTTGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000212 GO:0000226 GO:0000280 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005871 GO:0005872 GO:0005874 GO:0005875 GO:0005876 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007053 GO:0007140 GO:0007276 GO:0008017 GO:0008092 GO:0008150 GO:0008569 GO:0009524 GO:0009971 GO:0009987 GO:0015630 GO:0015631 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0022607 GO:0032501 GO:0032504 GO:0032991 GO:0042623 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0048232 GO:0048285 GO:0048609 GO:0051225 GO:0051321 GO:0051704 GO:0055048 GO:0070925 GO:0071840 GO:0090306 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:1903046 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin Microtub_bd

Protein Analysis

745

Amino Acids

84.36

Weight (kDa)

5.53

Isoelectric Point (pI)

43.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	369 - 534	5e-54	Microtubule binding
Kinesin	PF00225	393 - 723	2.3e-112	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006514)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27180 AT4G27180 AT5G54670 AT5G54670 AT5G54670
fragaria_vesca	FvH4_3g21681 FvH4_3g21681 FvH4_3g21681 FvH4_3g21681 FvH4_3g21681
malus_domestica	MD03G1212900.v1.1 MD11G1228000.v1.1
prunus_persica	Prupe.4G201200_v2.0.a1 Prupe.4G201200_v2.0.a1
pyrus_communis	pycom03g16170 pycom11g20070
rosa_chinensis	RchiOBHm_Chr5g0037181
rosa_laevigata	RLG00000033756
rosa_multiflora	Rmu_sc0030361.1_g000001
rosa_roxburghii	Rroxscaffold_1G00043800
rosa_rugosa	Rorug05G0161400
rosa_samantha	Rh5AG253000 Rh5BG254900 Rh5CG287600 Rh5DG263400
rosa_wichuraiana	Rw5G023370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	988, 1546
Acc36I	ACCTGC	2 cut(s)	988, 1546
AccBSI	CCGCTC	1 cut(s)	1920
AciI	CCGC	6 cut(s)	844, 1088, 1646, 1782, 1895, 1920
AclWI	GGATC	6 cut(s)	82, 735, 975, 1432, 1602, 1930
AcoI	YGGCCR	1 cut(s)	35
AcsI	RAATTY	1 cut(s)	1204
AcuI	CTGAAG	2 cut(s)	1172, 2039
AfaI	GTAC	5 cut(s)	146, 158, 566, 605, 1866
AfeI	AGCGCT	1 cut(s)	1378
AfiI	CCNNNNNNNGG	2 cut(s)	853, 2066
AflIII	ACRYGT	2 cut(s)	561, 1687
AgeI	ACCGGT	1 cut(s)	2123
AjnI	CCWGG	2 cut(s)	1456, 2204
Alw21I	GWGCWC	2 cut(s)	1853, 2141
Alw26I	GTCTC	5 cut(s)	809, 1177, 1527, 1980, 2136
AlwI	GGATC	6 cut(s)	82, 735, 975, 1432, 1602, 1930
AlwNI	CAGNNNCTG	2 cut(s)	1150, 1427
Ama87I	CYCGRG	2 cut(s)	236, 589
Aor51HI	AGCGCT	1 cut(s)	1378
AoxI	GGCC	5 cut(s)	35, 88, 1218, 1454, 2207
ApeKI	GCWGC	5 cut(s)	425, 485, 737, 1754, 2153
ApoI	RAATTY	1 cut(s)	1204
AsiGI	ACCGGT	1 cut(s)	2123
Asp700I	GAANNNNTTC	1 cut(s)	123
AspLEI	GCGC	2 cut(s)	305, 1379
AspS9I	GGNCC	1 cut(s)	89
AsuC2I	CCSGG	1 cut(s)	1466
AsuHPI	GGTGA	6 cut(s)	61, 635, 799, 1192, 2084, 2138
AsuII	TTCGAA	1 cut(s)	1717
AsuNHI	GCTAGC	1 cut(s)	502
AvaI	CYCGRG	2 cut(s)	236, 589
AxyI	CCTNAGG	1 cut(s)	2144
BbsI	GAAGAC	2 cut(s)	1784, 2087
Bbv12I	GWGCWC	2 cut(s)	1853, 2141
BbvI	GCAGC	5 cut(s)	412, 472, 724, 1766, 2165
BccI	CCATC	5 cut(s)	46, 181, 216, 1379, 1441
BceAI	ACGGC	1 cut(s)	2194
BcgI	CGANNNNNNTGC	2 cut(s)	1508, 1542
BciT130I	CCWGG	2 cut(s)	1458, 2206
BcnI	CCSGG	1 cut(s)	1466
BcoDI	GTCTC	5 cut(s)	809, 1177, 1527, 1980, 2136
BfaI	CTAG	6 cut(s)	503, 1209, 1247, 1674, 1803, 1979
BfmI	CTRYAG	3 cut(s)	722, 995, 1174
BfoI	RGCGCY	1 cut(s)	1380
BfuAI	ACCTGC	2 cut(s)	988, 1546
BglII	AGATCT	2 cut(s)	55, 1830
BisI	GCNGC	5 cut(s)	426, 486, 738, 1755, 2154
BlsI	GCNGC	5 cut(s)	427, 487, 739, 1756, 2155
Bme1390I	CCNGG	3 cut(s)	1458, 1466, 2206
BmeT110I	CYCGRG	2 cut(s)	236, 589
BmgT120I	GGNCC	1 cut(s)	89
BmrFI	CCNGG	3 cut(s)	1458, 1466, 2206
BmsI	GCATC	3 cut(s)	389, 1340, 1660
BmtI	GCTAGC	1 cut(s)	506
BpiI	GAAGAC	2 cut(s)	1784, 2087
BplI	GAGNNNNNCTC	2 cut(s)	572, 604
Bpu10I	CCTNAGC	2 cut(s)	206, 1999
Bpu14I	TTCGAA	1 cut(s)	1717
BpuEI	CTTGAG	3 cut(s)	791, 941, 1935
BpuMI	CCSGG	1 cut(s)	1466
BsaI	GGTCTC	1 cut(s)	1177
BsaJI	CCNNGG	1 cut(s)	2230
BsaWI	WCCGGW	2 cut(s)	46, 2123
Bsc4I	CCNNNNNNNGG	2 cut(s)	853, 2066
Bse118I	RCCGGY	1 cut(s)	2123
Bse1I	ACTGG	3 cut(s)	63, 679, 1933
Bse21I	CCTNAGG	1 cut(s)	2144
Bse3DI	GCAATG	2 cut(s)	298, 1791
BseBI	CCWGG	2 cut(s)	1458, 2206
BseDI	CCNNGG	1 cut(s)	2230
BseGI	GGATG	2 cut(s)	1161, 1474
BseLI	CCNNNNNNNGG	2 cut(s)	853, 2066
BseMI	GCAATG	2 cut(s)	298, 1791
BseMII	CTCAG	3 cut(s)	356, 975, 2158
BseNI	ACTGG	3 cut(s)	63, 679, 1933
BseRI	GAGGAG	3 cut(s)	353, 672, 1229
BseXI	GCAGC	5 cut(s)	412, 472, 724, 1766, 2165
BsgI	GTGCAG	1 cut(s)	2169
BshFI	GGCC	5 cut(s)	37, 90, 1220, 1456, 2209
BshTI	ACCGGT	1 cut(s)	2123
BsiHKAI	GWGCWC	2 cut(s)	1853, 2141
BsiHKCI	CYCGRG	2 cut(s)	236, 589
BsiSI	CCGG	4 cut(s)	34, 47, 1466, 2124
BslI	CCNNNNNNNGG	2 cut(s)	853, 2066
BsmAI	GTCTC	5 cut(s)	809, 1177, 1527, 1980, 2136
BsmBI	CGTCTC	1 cut(s)	1980
BsmI	GAATGC	1 cut(s)	2170
BsnI	GGCC	5 cut(s)	37, 90, 1220, 1456, 2209
Bso31I	GGTCTC	1 cut(s)	1177
BsoBI	CYCGRG	2 cut(s)	236, 589
Bsp119I	TTCGAA	1 cut(s)	1717
Bsp1286I	GDGCHC	2 cut(s)	1853, 2141
Bsp1407I	TGTACA	2 cut(s)	564, 603
BspACI	CCGC	6 cut(s)	844, 1088, 1646, 1782, 1895, 1920
BspANI	GGCC	5 cut(s)	37, 90, 1220, 1456, 2209
BspCNI	CTCAG	3 cut(s)	357, 976, 2157
BspHI	TCATGA	3 cut(s)	130, 640, 765
BspMAI	CTGCAG	1 cut(s)	999
BspMI	ACCTGC	2 cut(s)	988, 1546
BspOI	GCTAGC	1 cut(s)	506
BspPI	GGATC	6 cut(s)	82, 735, 975, 1432, 1602, 1930
BspQI	GCTCTTC	2 cut(s)	492, 1035
BspT104I	TTCGAA	1 cut(s)	1717
BspTNI	GGTCTC	1 cut(s)	1177
BsrBI	CCGCTC	1 cut(s)	1920
BsrDI	GCAATG	2 cut(s)	298, 1791
BsrFI	RCCGGY	1 cut(s)	2123
BsrGI	TGTACA	2 cut(s)	564, 603
BsrI	ACTGG	3 cut(s)	63, 679, 1933
BssAI	RCCGGY	1 cut(s)	2123
BssECI	CCNNGG	1 cut(s)	2230
BssT1I	CCWWGG	1 cut(s)	2230
Bst2UI	CCWGG	2 cut(s)	1458, 2206
Bst4CI	ACNGT	4 cut(s)	600, 839, 1705, 2192
Bst6I	CTCTTC	6 cut(s)	129, 330, 492, 951, 1035, 1530
BstAPI	GCANNNNNTGC	1 cut(s)	464
BstAUI	TGTACA	2 cut(s)	564, 603
BstBI	TTCGAA	1 cut(s)	1717
BstC8I	GCNNGC	4 cut(s)	483, 504, 735, 873
BstDEI	CTNAG	7 cut(s)	206, 365, 984, 1078, 1744, 1999, 2144
BstF5I	GGATG	2 cut(s)	1161, 1474
BstH2I	RGCGCY	1 cut(s)	1380
BstHHI	GCGC	2 cut(s)	305, 1379
BstMAI	GTCTC	5 cut(s)	809, 1177, 1527, 1980, 2136
BstMWI	GCNNNNNNNGC	3 cut(s)	464, 520, 1625
BstNI	CCWGG	2 cut(s)	1458, 2206
BstNSI	RCATGY	2 cut(s)	565, 1691
BstSCI	CCNGG	3 cut(s)	1456, 1464, 2204
BstSFI	CTRYAG	3 cut(s)	722, 995, 1174
BstV1I	GCAGC	5 cut(s)	412, 472, 724, 1766, 2165
BstV2I	GAAGAC	2 cut(s)	1784, 2087
BstX2I	RGATCY	5 cut(s)	55, 74, 1424, 1594, 1830
BstYI	RGATCY	5 cut(s)	55, 74, 1424, 1594, 1830
Bsu36I	CCTNAGG	1 cut(s)	2144
BsuRI	GGCC	5 cut(s)	37, 90, 1220, 1456, 2209
BtgZI	GCGATG	1 cut(s)	1998
BtsCI	GGATG	2 cut(s)	1161, 1474
BveI	ACCTGC	2 cut(s)	988, 1546
Cac8I	GCNNGC	4 cut(s)	483, 504, 735, 873
CaiI	CAGNNNCTG	2 cut(s)	1150, 1427
CciI	TCATGA	3 cut(s)	130, 640, 765
CfoI	GCGC	2 cut(s)	305, 1379
Cfr10I	RCCGGY	1 cut(s)	2123
Cfr13I	GGNCC	1 cut(s)	89
CseI	GACGC	2 cut(s)	520, 2092
Csp6I	GTAC	5 cut(s)	145, 157, 565, 604, 1865
CspAI	ACCGGT	1 cut(s)	2123
CviAII	CATG	7 cut(s)	131, 562, 641, 766, 1667, 1688, 2195
CviQI	GTAC	5 cut(s)	145, 157, 565, 604, 1865
DdeI	CTNAG	7 cut(s)	206, 365, 984, 1078, 1744, 1999, 2144
DraI	TTTAAA	1 cut(s)	715
EaeI	YGGCCR	1 cut(s)	35
Eam1104I	CTCTTC	6 cut(s)	129, 330, 492, 951, 1035, 1530
EarI	CTCTTC	6 cut(s)	129, 330, 492, 951, 1035, 1530
Eco130I	CCWWGG	1 cut(s)	2230
Eco31I	GGTCTC	1 cut(s)	1177
Eco47III	AGCGCT	1 cut(s)	1378
Eco57I	CTGAAG	2 cut(s)	1172, 2039
Eco81I	CCTNAGG	1 cut(s)	2144
Eco88I	CYCGRG	2 cut(s)	236, 589
EcoRI	GAATTC	1 cut(s)	1204
EcoRII	CCWGG	2 cut(s)	1456, 2204
EcoT14I	CCWWGG	1 cut(s)	2230
EcoT22I	ATGCAT	1 cut(s)	253
ErhI	CCWWGG	1 cut(s)	2230
Esp3I	CGTCTC	1 cut(s)	1980
FaeI	CATG	7 cut(s)	134, 565, 644, 769, 1670, 1691, 2198
FalI	AAGNNNNNCTT	4 cut(s)	507, 539, 1077, 1109
FatI	CATG	7 cut(s)	130, 561, 640, 765, 1666, 1687, 2194
FauI	CCCGC	1 cut(s)	1888
FauNDI	CATATG	1 cut(s)	1411
Fnu4HI	GCNGC	5 cut(s)	426, 486, 738, 1755, 2154
FokI	GGATG	2 cut(s)	1148, 1481
Fsp4HI	GCNGC	5 cut(s)	426, 486, 738, 1755, 2154
FspBI	CTAG	6 cut(s)	503, 1209, 1247, 1674, 1803, 1979
GlaI	GCGC	2 cut(s)	304, 1378
GluI	GCNGC	5 cut(s)	426, 486, 738, 1755, 2154
HaeII	RGCGCY	1 cut(s)	1380
HaeIII	GGCC	5 cut(s)	37, 90, 1220, 1456, 2209
HapII	CCGG	4 cut(s)	34, 47, 1466, 2124
HgaI	GACGC	2 cut(s)	520, 2092
HhaI	GCGC	2 cut(s)	305, 1379
Hin1II	CATG	7 cut(s)	134, 565, 644, 769, 1670, 1691, 2198
Hin6I	GCGC	2 cut(s)	303, 1377
HinP1I	GCGC	2 cut(s)	303, 1377
HincII	GTYRAC	2 cut(s)	1605, 2200
HindII	GTYRAC	2 cut(s)	1605, 2200
HindIII	AAGCTT	1 cut(s)	1966
HinfI	GANTC	9 cut(s)	30, 138, 434, 544, 817, 1483, 1839, 2075, 2129
HpaI	GTTAAC	1 cut(s)	2200
HpaII	CCGG	4 cut(s)	34, 47, 1466, 2124
HphI	GGTGA	6 cut(s)	61, 635, 799, 1192, 2084, 2138
Hpy166II	GTNNAC	4 cut(s)	102, 1605, 2098, 2200
Hpy188I	TCNGA	4 cut(s)	60, 79, 985, 2030
Hpy8I	GTNNAC	4 cut(s)	102, 1605, 2098, 2200
Hpy99I	CGWCG	1 cut(s)	635
HpyAV	CCTTC	5 cut(s)	65, 739, 1100, 1147, 2124
HpyCH4III	ACNGT	4 cut(s)	600, 839, 1705, 2192
HpyCH4IV	ACGT	1 cut(s)	2019
HpyF10VI	GCNNNNNNNGC	3 cut(s)	464, 520, 1625
HpyF3I	CTNAG	7 cut(s)	206, 365, 984, 1078, 1744, 1999, 2144
HpySE526I	ACGT	1 cut(s)	2019
Hsp92II	CATG	7 cut(s)	134, 565, 644, 769, 1670, 1691, 2198
HspAI	GCGC	2 cut(s)	303, 1377
KspAI	GTTAAC	1 cut(s)	2200
LguI	GCTCTTC	2 cut(s)	492, 1035
LmnI	GCTCC	4 cut(s)	340, 1063, 1498, 2144
Lsp1109I	GCAGC	5 cut(s)	412, 472, 724, 1766, 2165
LweI	GCATC	3 cut(s)	389, 1340, 1660
MaeI	CTAG	6 cut(s)	503, 1209, 1247, 1674, 1803, 1979
MaeII	ACGT	1 cut(s)	2019
MaeIII	GTNAC	4 cut(s)	750, 839, 1388, 2072
MbiI	CCGCTC	1 cut(s)	1920
MflI	RGATCY	5 cut(s)	55, 74, 1424, 1594, 1830
MhlI	GDGCHC	2 cut(s)	1853, 2141
MlyI	GAGTC	2 cut(s)	2069, 2138
MmeI	TCCRAC	1 cut(s)	1520
Mph1103I	ATGCAT	1 cut(s)	253
MroXI	GAANNNNTTC	1 cut(s)	123
MseI	TTAA	5 cut(s)	318, 701, 714, 1095, 2199
MslI	CAYNNNNRTG	1 cut(s)	2193
MspI	CCGG	4 cut(s)	34, 47, 1466, 2124
MspR9I	CCNGG	3 cut(s)	1458, 1466, 2206
Mva1269I	GAATGC	1 cut(s)	2170
MvaI	CCWGG	2 cut(s)	1458, 2206
MwoI	GCNNNNNNNGC	3 cut(s)	464, 520, 1625
NciI	CCSGG	1 cut(s)	1466
NdeI	CATATG	1 cut(s)	1411
NheI	GCTAGC	1 cut(s)	502
NlaIII	CATG	7 cut(s)	134, 565, 644, 769, 1670, 1691, 2198
NmuCI	GTSAC	3 cut(s)	750, 839, 2072
NsiI	ATGCAT	1 cut(s)	253
NspI	RCATGY	2 cut(s)	565, 1691
NspV	TTCGAA	1 cut(s)	1717
PaeR7I	CTCGAG	2 cut(s)	236, 589
PagI	TCATGA	3 cut(s)	130, 640, 765
PaqCI	CACCTGC	2 cut(s)	988, 1546
PciI	ACATGT	2 cut(s)	561, 1687
PciSI	GCTCTTC	2 cut(s)	492, 1035
PctI	GAATGC	1 cut(s)	2170
PdmI	GAANNNNTTC	1 cut(s)	123
PfeI	GAWTC	7 cut(s)	30, 138, 434, 544, 817, 1483, 1839
PinAI	ACCGGT	1 cut(s)	2123
PkrI	GCNGC	5 cut(s)	427, 487, 739, 1756, 2155
PleI	GAGTC	2 cut(s)	2069, 2137
PpsI	GAGTC	2 cut(s)	2069, 2137
PscI	ACATGT	2 cut(s)	561, 1687
Psp6I	CCWGG	2 cut(s)	1456, 2204
PspGI	CCWGG	2 cut(s)	1456, 2204
PspPI	GGNCC	1 cut(s)	89
PspXI	VCTCGAGB	2 cut(s)	236, 589
PstI	CTGCAG	1 cut(s)	999
PstNI	CAGNNNCTG	2 cut(s)	1150, 1427
PsuI	RGATCY	5 cut(s)	55, 74, 1424, 1594, 1830
RsaI	GTAC	5 cut(s)	146, 158, 566, 605, 1866
RsaNI	GTAC	5 cut(s)	145, 157, 565, 604, 1865
RseI	CAYNNNNRTG	1 cut(s)	2193
SapI	GCTCTTC	2 cut(s)	492, 1035
SaqAI	TTAA	5 cut(s)	318, 701, 714, 1095, 2199
SatI	GCNGC	5 cut(s)	426, 486, 738, 1755, 2154
Sau96I	GGNCC	1 cut(s)	89
SchI	GAGTC	2 cut(s)	2069, 2138
ScrFI	CCNGG	3 cut(s)	1458, 1466, 2206
SduI	GDGCHC	2 cut(s)	1853, 2141
SfaNI	GCATC	3 cut(s)	389, 1340, 1660
SfcI	CTRYAG	3 cut(s)	722, 995, 1174
Sfr274I	CTCGAG	2 cut(s)	236, 589
SfuI	TTCGAA	1 cut(s)	1717
SlaI	CTCGAG	2 cut(s)	236, 589
SmiMI	CAYNNNNRTG	1 cut(s)	2193
SmlI	CTYRAG	6 cut(s)	236, 589, 806, 920, 1950, 2224
SmoI	CTYRAG	6 cut(s)	236, 589, 806, 920, 1950, 2224
SsiI	CCGC	6 cut(s)	844, 1088, 1646, 1782, 1895, 1920
SspI	AATATT	1 cut(s)	1506
SspMI	CTAG	6 cut(s)	503, 1209, 1247, 1674, 1803, 1979
StyD4I	CCNGG	3 cut(s)	1456, 1464, 2204
StyI	CCWWGG	1 cut(s)	2230
TaaI	ACNGT	4 cut(s)	600, 839, 1705, 2192
TaiI	ACGT	1 cut(s)	2022
TatI	WGTACW	5 cut(s)	144, 156, 564, 603, 1864
TfiI	GAWTC	7 cut(s)	30, 138, 434, 544, 817, 1483, 1839
Tru1I	TTAA	5 cut(s)	318, 701, 714, 1095, 2199
Tru9I	TTAA	5 cut(s)	318, 701, 714, 1095, 2199
TseFI	GTSAC	3 cut(s)	750, 839, 2072
TseI	GCWGC	5 cut(s)	425, 485, 737, 1754, 2153
Tsp45I	GTSAC	3 cut(s)	750, 839, 2072
TspGWI	ACGGA	4 cut(s)	685, 869, 1135, 1154
XapI	RAATTY	1 cut(s)	1204
XbaI	TCTAGA	2 cut(s)	1246, 1802
XceI	RCATGY	2 cut(s)	565, 1691
XhoI	CTCGAG	2 cut(s)	236, 589
XmnI	GAANNNNTTC	1 cut(s)	123
XspI	CTAG	6 cut(s)	503, 1209, 1247, 1674, 1803, 1979
Zsp2I	ATGCAT	1 cut(s)	253

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.