Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0006514

Physical Location & Seq

Genomic Coordinates Forward (+)

32499530 .. 32506954

Gene Structure 7425 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG253000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2277 bp

ATGGTGAGCACGGCGAACAATTGGAGGTTCCGGCCGGCCTATGCTATGGTGAATGGGGGACCCGACTTGGCTCCGAATAGCGCTCCGGCGAGCAATGCCGGGTCGGAGTGCGGCGACGTTGAGTTTACCAGAGAATATGTTGAAGCATTGTTGAATGAAAGACCCAAAAGGAAGGACAGGTTCAGTCTCAAGGAAAAATGTGACTCTTTGACTGAATATATTAGAAGGCTGAAACCTGTTATAAGATGGTTTCAAGAGCTGGAGGGAAACTATTTATTGGAGCATGAGAAGTTACAGAACAGTTTGGAAGTGTCTGAGCTAAAACGCTCTGAAATGGATCTCTTACTGAAAAACAAGGAAGAGGAATTGAATTCGATTATTGCCGAGCTGAGGAAAAACTATGCTTCTTTACAAGAGAAATTTACTAAAGAAGAATTGGATAAGATGGCTGCAATGGATTCTCTCACCCGAGAAAGAGATGCTAGACTCGAAATTGAGCGATCCAAATATTCCCTCTCAGATGAGCTTGAAAGAGTTCAAAGAGACCTCACAAGTGCTAAGCAGCAGAATGCTTCACTCAGTGACATGTACAAACGACAGCAAGAATACATTGCAAATTTGCAACACTACAATAGTAAACTACAGACCGACCTCTCCGCAGTGGAGGAGGACCGTAAGCGTGTGGAGACTGAAAAGGCTAGTATGGTGGAGAATCTAGGCATGTATAGGGGTCAGCTTCAAGTGTCCAGACAATGTCAAGATGATGCAGTAAAGCAGAGGGATGCTTTGGCCAATGAAGTTGCTAGCCTTAAACTGGAGCTGCAACAAGCAAGAGATGATTGTGATCAACATCAATTGCAAGTACAGAGTTTAAGTGCTGAATTTACAGAGTATAAAGAGTCTACTGAAAAATCTTGTTATGAGTGGGGTAACCTCACTTCAAAAAATGCTGAACTGGAGGCAAGATGTCTGTTACAAAGTGACCAGATAAAGGAATTGCAGGACCAACTACTGACTGCAGAGAAGAAATTGCAGTTGTCCGATTTATCTACGATGGAAAGAAGAGCAGAATATGAAGGGCAAAAGAATGTTATAAGTGAGTTAAGAACTCGCCTGGCAGATGCAGAATTTAAACTTATTGAAGGAGAGATGCTGCGTAAAAAGTTGCACAATGAAAACTTGGAACTAAAAGGGAACATCCGCGTATTCTGTAGAGTCCGACCTTTTTTGGCTGAAGATGATTCTAATACAGAAGGGAAGGTTATTTCTTATCCAACATCAATGGATGCTGTTGGACGGGGGATTGAACTAGCGCAAAATGGGCAAAAGCATTCTTTTAAATTTGACAGAGTTTTTATTCCTGACATATCACAAGAAGATGTGTTCGAAGACATTTCACAACTCGTACAAAGTGCTCTTGATGGTTATAAGGTTTGCATCTTTGCTTATGGTCAAACAGGATCAGGCAAAACCTATACGATGATGGGTAAGCCGGGGGATCCAGAGCAAAAAGGGTTGATCCCTCGTACGTTGGAACAAATTTTTAAAACCAGACAATTTGCCGAACCACAAGGGTGGAAATATGAAATGCAGGTGTCAATGTTGGAAATCTACAATGAAACAATTCGTGATCTGTTAGCAACAAATAGATCATCTTTGGAAAGAACAGAAAATGGTACTGCTGGAAAGCAATATACAATCAAACATGATGCAAATGGAAACACCCATGTCTCTGATCTTACTGTTGTGGATGTGCGTAGTGCTAAAGAGGTTTCATATCTCTTAGAACGGGCTGCTCATAGCAGGTCTGTAGGCAAGACTCAAATGAATGAGCAATCATCAAGAAGCCATTTTGTTTTCACGCTACGCATATATGGTGTTAATGAGAGTACTGAACAACAAGTACAAGGCATCCTGAATCTTATTGATCTTGCGGGGAGTGAACGCCTTTCGAAGAGTGGATCAACTGGGGACCGATTGAAAGAAACTCAAGCCATCAACAAGAGTCTGTCATCTTTAAGTGACGTTATATTTGCCTTGGCAAAGAAGGAGGATCACGTTCCATTTAGGAACTCAAAGCTTACATATCTTCTTCAGCCATGTCTAGGTGGGGATTCGAAGACATTGATGTTTGTAAACATTGCTCCTGATCTCTCCTCAGCGGGTGAATCACTCTGTTCACTTCGTTTTGCTGCACGTGTAAATGCTTGTGAAATTGGGGTTCCACGACGTCAGACGTACACAAGAACTTCAGATTCTCGTTTGAGCTATGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008017 GO:0008092 GO:0008150 GO:0009987 GO:0015630 GO:0015631 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin Microtub_bd

Protein Analysis

758

Amino Acids

86.36

Weight (kDa)

5.67

Isoelectric Point (pI)

46.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	381 - 546	4.4e-52	Microtubule binding
Kinesin	PF00225	405 - 736	2.7e-112	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006514)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27180 AT4G27180 AT5G54670 AT5G54670 AT5G54670
fragaria_vesca	FvH4_3g21681 FvH4_3g21681 FvH4_3g21681 FvH4_3g21681 FvH4_3g21681
malus_domestica	MD03G1212900.v1.1 MD11G1228000.v1.1
prunus_persica	Prupe.4G201200_v2.0.a1 Prupe.4G201200_v2.0.a1
pyrus_communis	pycom03g16170 pycom11g20070
rosa_chinensis	RchiOBHm_Chr5g0037181
rosa_laevigata	RLG00000033756
rosa_multiflora	Rmu_sc0030361.1_g000001
rosa_roxburghii	Rroxscaffold_1G00043800
rosa_rugosa	Rorug05G0161400
rosa_samantha	Rh5AG253000 Rh5BG254900 Rh5CG287600 Rh5DG263400
rosa_wichuraiana	Rw5G023370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	242, 1094, 1428
AarI	CACCTGC	1 cut(s)	1582
AatII	GACGTC	1 cut(s)	2233
Acc36I	ACCTGC	2 cut(s)	1582, 1794
AccI	GTMKAC	1 cut(s)	902
AccII	CGCG	1 cut(s)	1203
AciI	CCGC	5 cut(s)	111, 657, 1201, 1934, 2162
AclWI	GGATC	8 cut(s)	345, 495, 1468, 1493, 1506, 1513, 1969, 2061
AcoI	YGGCCR	2 cut(s)	32, 789
AcsI	RAATTY	7 cut(s)	370, 419, 616, 881, 1127, 1340, 1539
AcuI	CTGAAG	3 cut(s)	1254, 2078, 2235
AcvI	CACGTG	1 cut(s)	2198
AcyI	GRCGYC	1 cut(s)	2230
AdeI	CACNNNGTG	1 cut(s)	581
AfaI	GTAC	8 cut(s)	590, 864, 1407, 1528, 1678, 1891, 1905, 2240
AfeI	AGCGCT	1 cut(s)	82
AfiI	CCNNNNNNNGG	4 cut(s)	390, 814, 991, 2105
AflIII	ACRYGT	2 cut(s)	585, 2197
AjnI	CCWGG	1 cut(s)	1113
AleI	CACNNNNGTG	1 cut(s)	1573
AluBI	AGCT	8 cut(s)	259, 319, 388, 526, 736, 820, 2080, 2268
AluI	AGCT	8 cut(s)	259, 319, 388, 526, 736, 820, 2080, 2268
Alw21I	GWGCWC	2 cut(s)	11, 1417
Alw26I	GTCTC	4 cut(s)	191, 537, 680, 1735
AlwI	GGATC	8 cut(s)	345, 495, 1468, 1493, 1506, 1513, 1969, 2061
Ama87I	CYCGRG	1 cut(s)	468
Aor51HI	AGCGCT	1 cut(s)	82
AoxI	GGCC	3 cut(s)	32, 36, 789
ApeKI	GCWGC	6 cut(s)	449, 562, 820, 1153, 1793, 2192
ApoI	RAATTY	7 cut(s)	370, 419, 616, 881, 1127, 1340, 1539
ArsI	GACNNNNNNTTYG	2 cut(s)	2015, 2047
Asp700I	GAANNNNTTC	2 cut(s)	534, 1623
AspLEI	GCGC	2 cut(s)	83, 1315
AspS9I	GGNCC	4 cut(s)	59, 670, 1003, 1972
AsuC2I	CCSGG	2 cut(s)	100, 1494
AsuHPI	GGTGA	4 cut(s)	16, 61, 457, 2177
AsuII	TTCGAA	3 cut(s)	1386, 1952, 2117
AsuNHI	GCTAGC	1 cut(s)	803
AvaI	CYCGRG	1 cut(s)	468
AvaII	GGWCC	4 cut(s)	59, 670, 1003, 1972
BalI	TGGCCA	1 cut(s)	791
BamHI	GGATCC	1 cut(s)	1498
BarI	GAAGNNNNNNTAC	2 cut(s)	922, 954
BbrPI	CACGTG	1 cut(s)	2198
BbsI	GAAGAC	2 cut(s)	1395, 2126
Bbv12I	GWGCWC	2 cut(s)	11, 1417
BbvCI	CCTCAGC	2 cut(s)	389, 2158
BbvI	GCAGC	6 cut(s)	436, 574, 807, 1140, 1780, 2179
BccI	CCATC	6 cut(s)	240, 439, 1048, 1415, 1477, 2003
BceAI	ACGGC	1 cut(s)	27
BciT130I	CCWGG	1 cut(s)	1115
BclI	TGATCA	1 cut(s)	844
BcnI	CCSGG	2 cut(s)	100, 1494
BcoDI	GTCTC	4 cut(s)	191, 537, 680, 1735
BfaI	CTAG	6 cut(s)	483, 699, 716, 804, 1310, 2105
BfmI	CTRYAG	4 cut(s)	641, 1017, 1210, 1809
BfoI	RGCGCY	1 cut(s)	84
BfuAI	ACCTGC	2 cut(s)	1582, 1794
BisI	GCNGC	7 cut(s)	112, 450, 563, 821, 1154, 1794, 2193
BlpI	GCTNAGC	1 cut(s)	558
BlsI	GCNGC	7 cut(s)	113, 451, 564, 822, 1155, 1795, 2194
BmcAI	AGTACT	1 cut(s)	1891
Bme1390I	CCNGG	3 cut(s)	100, 1115, 1494
Bme18I	GGWCC	4 cut(s)	59, 670, 1003, 1972
BmeT110I	CYCGRG	1 cut(s)	468
BmgT120I	GGNCC	4 cut(s)	59, 670, 1003, 1972
BmiI	GGNNCC	7 cut(s)	29, 60, 61, 72, 1500, 1973, 2223
BmrFI	CCNGG	3 cut(s)	100, 1115, 1494
BmrI	ACTGGG	1 cut(s)	1977
BmsI	GCATC	9 cut(s)	469, 754, 772, 1111, 1140, 1276, 1446, 1699, 1920
BmtI	GCTAGC	1 cut(s)	807
BmuI	ACTGGG	1 cut(s)	1977
BpiI	GAAGAC	2 cut(s)	1395, 2126
BpmI	CTGGAG	3 cut(s)	281, 836, 977
Bpu10I	CCTNAGC	2 cut(s)	389, 2158
Bpu1102I	GCTNAGC	1 cut(s)	558
Bpu14I	TTCGAA	3 cut(s)	1386, 1952, 2117
BpuEI	CTTGAG	2 cut(s)	173, 1974
BpuMI	CCSGG	2 cut(s)	100, 1494
BsaAI	YACGTR	1 cut(s)	2198
BsaBI	GATNNNNATC	2 cut(s)	843, 849
BsaHI	GRCGYC	1 cut(s)	2230
BsaI	GGTCTC	1 cut(s)	537
BsaJI	CCNNGG	2 cut(s)	1493, 2037
Bsc4I	CCNNNNNNNGG	4 cut(s)	390, 814, 991, 2105
Bse118I	RCCGGY	1 cut(s)	34
Bse1I	ACTGG	3 cut(s)	819, 960, 1972
Bse3DI	GCAATG	4 cut(s)	100, 459, 609, 2139
Bse8I	GATNNNNATC	2 cut(s)	843, 849
BseBI	CCWGG	1 cut(s)	1115
BseDI	CCNNGG	2 cut(s)	1493, 2037
BseGI	GGATG	5 cut(s)	787, 1197, 1291, 1756, 1911
BseJI	GATNNNNATC	2 cut(s)	843, 849
BseLI	CCNNNNNNNGG	4 cut(s)	390, 814, 991, 2105
BseMI	GCAATG	4 cut(s)	100, 459, 609, 2139
BseMII	CTCAG	5 cut(s)	306, 380, 531, 592, 2172
BseNI	ACTGG	3 cut(s)	819, 960, 1972
BseRI	GAGGAG	2 cut(s)	680, 2146
BseX3I	CGGCCG	1 cut(s)	32
BseXI	GCAGC	6 cut(s)	436, 574, 807, 1140, 1780, 2179
BsgI	GTGCAG	1 cut(s)	2178
Bsh1236I	CGCG	1 cut(s)	1203
Bsh1285I	CGRYCG	1 cut(s)	35
BshFI	GGCC	3 cut(s)	34, 38, 791
BsiEI	CGRYCG	1 cut(s)	35
BsiHKAI	GWGCWC	2 cut(s)	11, 1417
BsiHKCI	CYCGRG	1 cut(s)	468
BsiSI	CCGG	5 cut(s)	31, 35, 86, 99, 1493
BsiWI	CGTACG	1 cut(s)	1526
BslFI	GGGAC	2 cut(s)	72, 1985
BslI	CCNNNNNNNGG	4 cut(s)	390, 814, 991, 2105
BsmAI	GTCTC	4 cut(s)	191, 537, 680, 1735
BsmFI	GGGAC	2 cut(s)	72, 1985
BsmI	GAATGC	2 cut(s)	574, 1330
BsnI	GGCC	3 cut(s)	34, 38, 791
Bso31I	GGTCTC	1 cut(s)	537
BsoBI	CYCGRG	1 cut(s)	468
Bsp119I	TTCGAA	3 cut(s)	1386, 1952, 2117
Bsp1286I	GDGCHC	2 cut(s)	11, 1417
Bsp1407I	TGTACA	1 cut(s)	588
Bsp1720I	GCTNAGC	1 cut(s)	558
BspACI	CCGC	5 cut(s)	111, 657, 1201, 1934, 2162
BspANI	GGCC	3 cut(s)	34, 38, 791
BspCNI	CTCAG	5 cut(s)	307, 381, 530, 591, 2171
BspFNI	CGCG	1 cut(s)	1203
BspLI	GGNNCC	7 cut(s)	29, 60, 61, 72, 1500, 1973, 2223
BspMAI	CTGCAG	1 cut(s)	1021
BspMI	ACCTGC	2 cut(s)	1582, 1794
BspOI	GCTAGC	1 cut(s)	807
BspPI	GGATC	8 cut(s)	345, 495, 1468, 1493, 1506, 1513, 1969, 2061
BspQI	GCTCTTC	1 cut(s)	1057
BspT104I	TTCGAA	3 cut(s)	1386, 1952, 2117
BspTNI	GGTCTC	1 cut(s)	537
BsrDI	GCAATG	4 cut(s)	100, 459, 609, 2139
BsrFI	RCCGGY	1 cut(s)	34
BsrGI	TGTACA	1 cut(s)	588
BsrI	ACTGG	3 cut(s)	819, 960, 1972
BssAI	RCCGGY	1 cut(s)	34
BssECI	CCNNGG	2 cut(s)	1493, 2037
BssNI	GRCGYC	1 cut(s)	2230
BssT1I	CCWWGG	1 cut(s)	2037
Bst2UI	CCWGG	1 cut(s)	1115
Bst4CI	ACNGT	3 cut(s)	302, 674, 1744
Bst6I	CTCTTC	3 cut(s)	354, 1057, 1949
BstACI	GRCGYC	1 cut(s)	2230
BstAUI	TGTACA	1 cut(s)	588
BstBAI	YACGTR	1 cut(s)	2198
BstBI	TTCGAA	3 cut(s)	1386, 1952, 2117
BstC8I	GCNNGC	3 cut(s)	36, 91, 805
BstDEI	CTNAG	7 cut(s)	315, 389, 517, 558, 578, 1783, 2158
BstEII	GGTNACC	1 cut(s)	929
BstF5I	GGATG	5 cut(s)	787, 1197, 1291, 1756, 1911
BstFNI	CGCG	1 cut(s)	1203
BstH2I	RGCGCY	1 cut(s)	84
BstHHI	GCGC	2 cut(s)	83, 1315
BstMAI	GTCTC	4 cut(s)	191, 537, 680, 1735
BstMCI	CGRYCG	1 cut(s)	35
BstMWI	GCNNNNNNNGC	2 cut(s)	95, 1321
BstNI	CCWGG	1 cut(s)	1115
BstNSI	RCATGY	2 cut(s)	589, 724
BstPI	GGTNACC	1 cut(s)	929
BstSCI	CCNGG	3 cut(s)	98, 1113, 1492
BstSFI	CTRYAG	4 cut(s)	641, 1017, 1210, 1809
BstUI	CGCG	1 cut(s)	1203
BstV1I	GCAGC	6 cut(s)	436, 574, 807, 1140, 1780, 2179
BstV2I	GAAGAC	2 cut(s)	1395, 2126
BstX2I	RGATCY	2 cut(s)	337, 1498
BstXI	CCANNNNNNTGG	1 cut(s)	1575
BstYI	RGATCY	2 cut(s)	337, 1498
BstZI	CGGCCG	1 cut(s)	32
BsuRI	GGCC	3 cut(s)	34, 38, 791
BtsCI	GGATG	5 cut(s)	787, 1197, 1291, 1756, 1911
BtsI	GCAGTG	1 cut(s)	666
BtsIMutI	CAGTG	2 cut(s)	586, 666
BveI	ACCTGC	2 cut(s)	1582, 1794
Cac8I	GCNNGC	3 cut(s)	36, 91, 805
CfoI	GCGC	2 cut(s)	83, 1315
Cfr10I	RCCGGY	1 cut(s)	34
Cfr13I	GGNCC	4 cut(s)	59, 670, 1003, 1972
Csp6I	GTAC	8 cut(s)	589, 863, 1406, 1527, 1677, 1890, 1904, 2239
CviAII	CATG	6 cut(s)	284, 586, 721, 1706, 1727, 2100
CviQI	GTAC	8 cut(s)	589, 863, 1406, 1527, 1677, 1890, 1904, 2239
DdeI	CTNAG	7 cut(s)	315, 389, 517, 558, 578, 1783, 2158
DraI	TTTAAA	3 cut(s)	1132, 1339, 1546
DraIII	CACNNNGTG	1 cut(s)	581
EaeI	YGGCCR	2 cut(s)	32, 789
EagI	CGGCCG	1 cut(s)	32
Eam1104I	CTCTTC	3 cut(s)	354, 1057, 1949
EarI	CTCTTC	3 cut(s)	354, 1057, 1949
EclXI	CGGCCG	1 cut(s)	32
Eco130I	CCWWGG	1 cut(s)	2037
Eco31I	GGTCTC	1 cut(s)	537
Eco47I	GGWCC	4 cut(s)	59, 670, 1003, 1972
Eco47III	AGCGCT	1 cut(s)	82
Eco52I	CGGCCG	1 cut(s)	32
Eco57I	CTGAAG	3 cut(s)	1254, 2078, 2235
Eco72I	CACGTG	1 cut(s)	2198
Eco88I	CYCGRG	1 cut(s)	468
Eco91I	GGTNACC	1 cut(s)	929
EcoO109I	RGGNCCY	1 cut(s)	59
EcoO65I	GGTNACC	1 cut(s)	929
EcoRI	GAATTC	1 cut(s)	370
EcoRII	CCWGG	1 cut(s)	1113
EcoT14I	CCWWGG	1 cut(s)	2037
ErhI	CCWWGG	1 cut(s)	2037
FaeI	CATG	6 cut(s)	287, 589, 724, 1709, 1730, 2103
FaqI	GGGAC	2 cut(s)	72, 1985
FatI	CATG	6 cut(s)	283, 585, 720, 1705, 1726, 2099
FauI	CCCGC	2 cut(s)	1927, 2155
FbaI	TGATCA	1 cut(s)	844
FblI	GTMKAC	1 cut(s)	902
Fnu4HI	GCNGC	7 cut(s)	112, 450, 563, 821, 1154, 1794, 2193
FokI	GGATG	5 cut(s)	794, 1184, 1298, 1763, 1898
FseI	GGCCGGCC	1 cut(s)	38
Fsp4HI	GCNGC	7 cut(s)	112, 450, 563, 821, 1154, 1794, 2193
FspBI	CTAG	6 cut(s)	483, 699, 716, 804, 1310, 2105
GlaI	GCGC	2 cut(s)	82, 1314
GluI	GCNGC	7 cut(s)	112, 450, 563, 821, 1154, 1794, 2193
GsuI	CTGGAG	3 cut(s)	281, 836, 977
HaeII	RGCGCY	1 cut(s)	84
HaeIII	GGCC	3 cut(s)	34, 38, 791
HapII	CCGG	5 cut(s)	31, 35, 86, 99, 1493
HhaI	GCGC	2 cut(s)	83, 1315
Hin1I	GRCGYC	1 cut(s)	2230
Hin1II	CATG	6 cut(s)	287, 589, 724, 1709, 1730, 2103
Hin6I	GCGC	2 cut(s)	81, 1313
HinP1I	GCGC	2 cut(s)	81, 1313
HindIII	AAGCTT	1 cut(s)	2078
HpaII	CCGG	5 cut(s)	31, 35, 86, 99, 1493
HphI	GGTGA	4 cut(s)	16, 61, 457, 2177
Hpy166II	GTNNAC	7 cut(s)	126, 638, 903, 1943, 2137, 2180, 2241
Hpy8I	GTNNAC	7 cut(s)	126, 638, 903, 1943, 2137, 2180, 2241
Hpy99I	CGWCG	2 cut(s)	119, 2232
HpyAV	CCTTC	7 cut(s)	166, 219, 1070, 1136, 1247, 1252, 2041
HpyCH4III	ACNGT	3 cut(s)	302, 674, 1744
HpyCH4IV	ACGT	7 cut(s)	117, 1529, 2025, 2058, 2197, 2230, 2237
HpyF10VI	GCNNNNNNNGC	2 cut(s)	95, 1321
HpyF3I	CTNAG	7 cut(s)	315, 389, 517, 558, 578, 1783, 2158
HpySE526I	ACGT	7 cut(s)	117, 1529, 2025, 2058, 2197, 2230, 2237
Hsp92I	GRCGYC	1 cut(s)	2230
Hsp92II	CATG	6 cut(s)	287, 589, 724, 1709, 1730, 2103
HspAI	GCGC	2 cut(s)	81, 1313
KflI	GGGWCCC	1 cut(s)	59
KroI	GCCGGC	1 cut(s)	34
KroNI	GCCGGC	1 cut(s)	36
Ksp22I	TGATCA	1 cut(s)	844
LguI	GCTCTTC	1 cut(s)	1057
LmnI	GCTCC	5 cut(s)	76, 88, 280, 817, 2149
Lsp1109I	GCAGC	6 cut(s)	436, 574, 807, 1140, 1780, 2179
LweI	GCATC	9 cut(s)	469, 754, 772, 1111, 1140, 1276, 1446, 1699, 1920
MaeI	CTAG	6 cut(s)	483, 699, 716, 804, 1310, 2105
MaeII	ACGT	7 cut(s)	117, 1529, 2025, 2058, 2197, 2230, 2237
MaeIII	GTNAC	7 cut(s)	200, 291, 581, 929, 972, 980, 2021
MfeI	CAATTG	2 cut(s)	19, 854
MflI	RGATCY	2 cut(s)	337, 1498
MhlI	GDGCHC	2 cut(s)	11, 1417
MlsI	TGGCCA	1 cut(s)	791
MluNI	TGGCCA	1 cut(s)	791
MlyI	GAGTC	6 cut(s)	197, 480, 908, 1224, 1813, 2014
MmeI	TCCRAC	6 cut(s)	84, 1243, 1273, 1298, 1512, 1584
Mox20I	TGGCCA	1 cut(s)	791
MroNI	GCCGGC	1 cut(s)	34
MroXI	GAANNNNTTC	2 cut(s)	534, 1623
MscI	TGGCCA	1 cut(s)	791
MseI	TTAA	8 cut(s)	810, 872, 1103, 1131, 1338, 1545, 1881, 2018
MslI	CAYNNNNRTG	1 cut(s)	1573
Msp20I	TGGCCA	1 cut(s)	791
MspA1I	CMGCKG	1 cut(s)	2162
MspI	CCGG	5 cut(s)	31, 35, 86, 99, 1493
MspR9I	CCNGG	3 cut(s)	100, 1115, 1494
MunI	CAATTG	2 cut(s)	19, 854
Mva1269I	GAATGC	2 cut(s)	574, 1330
MvaI	CCWGG	1 cut(s)	1115
MvnI	CGCG	1 cut(s)	1203
MwoI	GCNNNNNNNGC	2 cut(s)	95, 1321
NaeI	GCCGGC	1 cut(s)	36
NciI	CCSGG	2 cut(s)	100, 1494
NgoMIV	GCCGGC	1 cut(s)	34
NheI	GCTAGC	1 cut(s)	803
NlaIII	CATG	6 cut(s)	287, 589, 724, 1709, 1730, 2103
NlaIV	GGNNCC	7 cut(s)	29, 60, 61, 72, 1500, 1973, 2223
NmeAIII	GCCGAG	1 cut(s)	409
NmuCI	GTSAC	4 cut(s)	200, 581, 980, 2021
NspI	RCATGY	2 cut(s)	589, 724
NspV	TTCGAA	3 cut(s)	1386, 1952, 2117
OliI	CACNNNNGTG	1 cut(s)	1573
PaqCI	CACCTGC	1 cut(s)	1582
PciI	ACATGT	1 cut(s)	585
PciSI	GCTCTTC	1 cut(s)	1057
PctI	GAATGC	2 cut(s)	574, 1330
PdiI	GCCGGC	1 cut(s)	36
PdmI	GAANNNNTTC	2 cut(s)	534, 1623
PfeI	GAWTC	7 cut(s)	458, 712, 1241, 1918, 2114, 2168, 2255
Pfl23II	CGTACG	1 cut(s)	1526
PflFI	GACNNNGTC	1 cut(s)	753
PkrI	GCNGC	7 cut(s)	113, 451, 564, 822, 1155, 1795, 2194
PleI	GAGTC	6 cut(s)	197, 480, 907, 1223, 1813, 2013
PmaCI	CACGTG	1 cut(s)	2198
PmlI	CACGTG	1 cut(s)	2198
PpsI	GAGTC	6 cut(s)	197, 480, 907, 1223, 1813, 2013
Ppu21I	YACGTR	1 cut(s)	2198
PpuMI	RGGWCCY	1 cut(s)	59
PscI	ACATGT	1 cut(s)	585
PsiI	TTATAA	3 cut(s)	242, 1094, 1428
Psp5II	RGGWCCY	1 cut(s)	59
Psp6I	CCWGG	1 cut(s)	1113
PspCI	CACGTG	1 cut(s)	2198
PspEI	GGTNACC	1 cut(s)	929
PspGI	CCWGG	1 cut(s)	1113
PspLI	CGTACG	1 cut(s)	1526
PspN4I	GGNNCC	7 cut(s)	29, 60, 61, 72, 1500, 1973, 2223
PspPI	GGNCC	4 cut(s)	59, 670, 1003, 1972
PspPPI	RGGWCCY	1 cut(s)	59
PsrI	GAACNNNNNNTAC	2 cut(s)	1887, 1919
PstI	CTGCAG	1 cut(s)	1021
PsuI	RGATCY	2 cut(s)	337, 1498
PsyI	GACNNNGTC	1 cut(s)	753
RigI	GGCCGGCC	1 cut(s)	38
RsaI	GTAC	8 cut(s)	590, 864, 1407, 1528, 1678, 1891, 1905, 2240
RsaNI	GTAC	8 cut(s)	589, 863, 1406, 1527, 1677, 1890, 1904, 2239
RseI	CAYNNNNRTG	1 cut(s)	1573
SapI	GCTCTTC	1 cut(s)	1057
SaqAI	TTAA	8 cut(s)	810, 872, 1103, 1131, 1338, 1545, 1881, 2018
SatI	GCNGC	7 cut(s)	112, 450, 563, 821, 1154, 1794, 2193
Sau96I	GGNCC	4 cut(s)	59, 670, 1003, 1972
ScaI	AGTACT	1 cut(s)	1891
SchI	GAGTC	6 cut(s)	197, 480, 908, 1224, 1813, 2014
ScrFI	CCNGG	3 cut(s)	100, 1115, 1494
SduI	GDGCHC	2 cut(s)	11, 1417
SfaNI	GCATC	9 cut(s)	469, 754, 772, 1111, 1140, 1276, 1446, 1699, 1920
SfcI	CTRYAG	4 cut(s)	641, 1017, 1210, 1809
SfuI	TTCGAA	3 cut(s)	1386, 1952, 2117
SinI	GGWCC	4 cut(s)	59, 670, 1003, 1972
SmiMI	CAYNNNNRTG	1 cut(s)	1573
SmlI	CTYRAG	2 cut(s)	188, 1989
SmoI	CTYRAG	2 cut(s)	188, 1989
SsiI	CCGC	5 cut(s)	111, 657, 1201, 1934, 2162
SspI	AATATT	1 cut(s)	509
SspMI	CTAG	6 cut(s)	483, 699, 716, 804, 1310, 2105
StyD4I	CCNGG	3 cut(s)	98, 1113, 1492
StyI	CCWWGG	1 cut(s)	2037
TaaI	ACNGT	3 cut(s)	302, 674, 1744
TaiI	ACGT	7 cut(s)	120, 1532, 2028, 2061, 2200, 2233, 2240
TaqI	TCGA	5 cut(s)	374, 489, 1386, 1952, 2117
TaqII	GACCGA	2 cut(s)	662, 1989
TatI	WGTACW	4 cut(s)	588, 862, 1889, 1903
TauI	GCSGC	1 cut(s)	114
TfiI	GAWTC	7 cut(s)	458, 712, 1241, 1918, 2114, 2168, 2255
Tru1I	TTAA	8 cut(s)	810, 872, 1103, 1131, 1338, 1545, 1881, 2018
Tru9I	TTAA	8 cut(s)	810, 872, 1103, 1131, 1338, 1545, 1881, 2018
TscAI	CASTG	2 cut(s)	586, 666
TseFI	GTSAC	4 cut(s)	200, 581, 980, 2021
TseI	GCWGC	6 cut(s)	449, 562, 820, 1153, 1793, 2192
Tsp45I	GTSAC	4 cut(s)	200, 581, 980, 2021
TspDTI	ATGAA	8 cut(s)	171, 810, 1089, 1188, 1599, 1632, 1764, 1841
TspRI	CASTG	2 cut(s)	586, 666
Tth111I	GACNNNGTC	1 cut(s)	753
VpaK11BI	GGWCC	4 cut(s)	59, 670, 1003, 1972
XapI	RAATTY	7 cut(s)	370, 419, 616, 881, 1127, 1340, 1539
XceI	RCATGY	2 cut(s)	589, 724
XmiI	GTMKAC	1 cut(s)	902
XmnI	GAANNNNTTC	2 cut(s)	534, 1623
XspI	CTAG	6 cut(s)	483, 699, 716, 804, 1310, 2105
ZraI	GACGTC	1 cut(s)	2231
ZrmI	AGTACT	1 cut(s)	1891

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.