Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0006514

Physical Location & Seq

Genomic Coordinates Reverse (-)

33178277 .. 33183816

Gene Structure 5540 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1228000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2316 bp

ATGGTGGGCACTGCGCAAAATTGGAGGGTCCGGCACGCGTTCTCGGTGGTGAACGGGGGCCAAGACATGGGTCCGAGCAGCGCTCCGGCGAGTAATGCCGGTTCTGAGAGCGGTGGTATTGAGTTTACCAGAGAGGATGTTGACGCATTATTGAATGAGAGACCCAAAAGGAAGGACAGATTCAGTCTCAAGGAAAAATGTGAGACACTGAGTGAGTATGTTAAAAGGCTGAAACTTTGCATAAAGTGGTTCCAAGAGCTTGAGGAAAGCTACTTATTAGAGCACGAGAAGTTAGAGAATGATGTACAAGTGTCAAAGCTGCAACGCGATGAAATGGGGATCTTACTGGAAAACAAGGAAGAAGAATTGAATTCAATTATTGTGGAGCTCAGGAAAAACTATGCTTCTTTACAAGAGAAATTTACGAAAGAAGAGTTGGATAAGATGGCTGCAATGGAATCTCTCTCTAGGGAAAGAGAGGCTAGACGTGAAATTGAGCGATCACAAAATTCCCTCTCAGATGAGCACGCGAGAGTTCAAAGAGAGCTTTCAAGTGCTACTCAGAAGATAACTTCACTGAATGATATGTACAAACGATTGCAAGAATACATTGCAAGCTTGCAGCAGTACAACAGTAAACTGCACAACGACCTCTCTACAGTTGAGGAAGACTTTAAGCGCGTAGAGAAGGAAAAAGTTAGTATGGTGGAGAACCTGAGCATGTTAAGGGGTCAGCTTTCAGTGTCCAGACAAACTCAAGATGATGCTATAAAGCAGAGGGATGCTTTGGTAAATGAAGTTGCATCCCTTAGGATGGAGTTGCAACAAGTGAGAGGTGATCGGGATCGACATAAACTCCAAGTGCAGACCCTAACAGCTGAATATACAACGTACAAAGAATCCACCGAGAAATCTAGTTTTGAGCTTGATGACCTCAGATCAAAATACACCGTACTGGAGGTAAGATGTCTGTCTCAGAGTGACCAGATAAGGACATTGAAGGACCAACTAATGATTGCAGAGGAGAAAGTACAGATGTCCGACTTATCTACAATGGAAACAAGAACTGAATATGAAGGGCAAAAGAAACTTATTAGTGAGTTACAGACTCGCCTGGCAGATGCAGAATTCAAACTTATTGAAGGAGAAATGTTGCGTAAAAAGTTGCACAACACTATCTTGGAACTAAAAGGGAACATCCGTGTATTCTGTCGAGTGCGACCTTTGTTGGCGGATGAAGACTTAAGCACTGAAGGGAAGGTCATCTCTTATCCAACATCAATGGATGCTCTTGGACGGGGCATTGATTTAGTGCAACCTGGGCAAAAACATTCTTTCACATTTGACAAAGTTTTTATGCCTGAAATATCACAAGAAGATGTGTTTGAAGACATTTCACAGCTTGTACAAAGTGCTCTTGATGGTTATAAGGTTTGCATTTTTGCCTATGGTCAGACAGGATCAGGGAAAACCTATACAATGATGGGGCAGCCAGGAGATCCAGACCAAAAAGGATTGATTCCTCGTTCATTAGAACAAATTTTTAAAACCAGACAATCTCTTCAACCACAAGGTTGGAAATATGAGATGCAGGTGTCGATGTTGGAAATATATAATGAAACTATTCGTGATCTGTTAGCACCCAATCGATCATCTTCAGATATGTTGCGAGAAAATGGTATTGGTGGAAAGCAATATACAATCAAACATGATGCAAATGGGAACACTCATGTCTCTGATCTTACCATTGTGGATGTGCGCACTGCTAAAGAGGTCTCATATCTCTTAGAACGGGCTGCACATAGCAGGTCTGTAGGCAAAACTCAAATGAACGAGCAATCTTCAAGAAGCCATTTTGTTTTCACACTACGAATATCTGGCGTTAATGAGGTAAGCAACATCCATTTTCTCTGCAGATACTCTTCTAGCACTGAACAACAAGTACAAGGTGTCCTGAATCTTATTGATCTTGCTGGGAGTGAACGACTTTCCAAGAGTGGGTCAACGGGGGACCGACTGAAAGAAACTCAGGCCATCAACAAAAGTCTCTCATGTTTAAGCGACGTAATATTTGCCTTGGCAAAGAAGGAGGACCACGTACCGTTTAGGAACTCAAAGCTTACATATCTTCTTCAGCCATGTCTCGGAGGAGACTCAAAGACATTGATGTTCGTAAACATTTCTCCCGATCCATCCTCAGCAGGTGAATCACTCTGTTCGCTTCGCTTTGCTTCACGGGTAAATGCTTGTGATATTGGGGTTCCACGACGTCAGACCAACATGCGGCCTCCAGATTCACGTATAAGCTATGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008017 GO:0008092 GO:0008150 GO:0009987 GO:0015630 GO:0015631 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin Microtub_bd

Protein Analysis

772

Amino Acids

87.74

Weight (kDa)

5.98

Isoelectric Point (pI)

47.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	381 - 546	2.3e-54	Microtubule binding
Kinesin	PF00225	405 - 749	1.4e-110	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006514)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27180 AT4G27180 AT5G54670 AT5G54670 AT5G54670
fragaria_vesca	FvH4_3g21681 FvH4_3g21681 FvH4_3g21681 FvH4_3g21681 FvH4_3g21681
malus_domestica	MD03G1212900.v1.1 MD11G1228000.v1.1
prunus_persica	Prupe.4G201200_v2.0.a1 Prupe.4G201200_v2.0.a1
pyrus_communis	pycom03g16170 pycom11g20070
rosa_chinensis	RchiOBHm_Chr5g0037181
rosa_laevigata	RLG00000033756
rosa_multiflora	Rmu_sc0030361.1_g000001
rosa_roxburghii	Rroxscaffold_1G00043800
rosa_rugosa	Rorug05G0161400
rosa_samantha	Rh5AG253000 Rh5BG254900 Rh5CG287600 Rh5DG263400
rosa_wichuraiana	Rw5G023370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1428
AarI	CACCTGC	2 cut(s)	1582, 2192
AatII	GACGTC	1 cut(s)	2272
Acc16I	TGCGCA	2 cut(s)	15, 1760
Acc36I	ACCTGC	3 cut(s)	1582, 1797, 2192
AccB7I	CCANNNNNTGG	1 cut(s)	67
AccBSI	CCGCTC	1 cut(s)	111
AccII	CGCG	4 cut(s)	38, 327, 530, 681
AciI	CCGC	3 cut(s)	111, 1232, 2284
AclWI	GGATC	5 cut(s)	347, 852, 1468, 1493, 2183
AcsI	RAATTY	5 cut(s)	370, 419, 508, 1127, 1539
AcuI	CTGAAG	3 cut(s)	1272, 1641, 2117
AcyI	GRCGYC	1 cut(s)	2269
AdeI	CACNNNGTG	1 cut(s)	212
AfaI	GTAC	9 cut(s)	306, 590, 629, 893, 954, 1032, 1407, 1944, 2100
AfeI	AGCGCT	1 cut(s)	82
AfiI	CCNNNNNNNGG	5 cut(s)	67, 814, 1998, 2144, 2283
AflII	CTTAAG	1 cut(s)	1243
AflIII	ACRYGT	1 cut(s)	36
AjiI	CACGTC	1 cut(s)	488
AjnI	CCWGG	3 cut(s)	1113, 1318, 1492
AjuI	GAANNNNNNNTTGG	4 cut(s)	419, 451, 1665, 1697
Alw21I	GWGCWC	4 cut(s)	285, 390, 528, 1417
Alw26I	GTCTC	9 cut(s)	154, 191, 197, 978, 1738, 1780, 2051, 2145, 2147
AlwI	GGATC	5 cut(s)	347, 852, 1468, 1493, 2183
Aor51HI	AGCGCT	1 cut(s)	82
AoxI	GGCC	3 cut(s)	58, 2031, 2285
ApeKI	GCWGC	6 cut(s)	78, 319, 449, 622, 1489, 1796
ApoI	RAATTY	5 cut(s)	370, 419, 508, 1127, 1539
ArsI	GACNNNNNNTTYG	2 cut(s)	2054, 2086
Asp700I	GAANNNNTTC	1 cut(s)	1623
AspLEI	GCGC	4 cut(s)	16, 83, 681, 1761
AspS9I	GGNCC	6 cut(s)	28, 58, 71, 1003, 2011, 2092
AsuHPI	GGTGA	3 cut(s)	61, 848, 2216
AvaII	GGWCC	5 cut(s)	28, 71, 1003, 2011, 2092
AxyI	CCTNAGG	1 cut(s)	809
BaeGI	GKGCMC	1 cut(s)	11
BanII	GRGCYC	1 cut(s)	390
BauI	CACGAG	1 cut(s)	284
BbsI	GAAGAC	3 cut(s)	675, 1245, 1395
Bbv12I	GWGCWC	4 cut(s)	285, 390, 528, 1417
BbvCI	CCTCAGC	1 cut(s)	2197
BbvI	GCAGC	6 cut(s)	90, 306, 436, 634, 1501, 1783
BccI	CCATC	6 cut(s)	439, 808, 1415, 1477, 2042, 2200
BciT130I	CCWGG	3 cut(s)	1115, 1320, 1494
BcoDI	GTCTC	9 cut(s)	154, 191, 197, 978, 1738, 1780, 2051, 2145, 2147
BfaI	CTAG	4 cut(s)	468, 483, 915, 1926
BfmI	CTRYAG	3 cut(s)	657, 1812, 1912
BfoI	RGCGCY	1 cut(s)	84
BfrI	CTTAAG	1 cut(s)	1243
BfuAI	ACCTGC	3 cut(s)	1582, 1797, 2192
BisI	GCNGC	7 cut(s)	79, 320, 450, 623, 1490, 1797, 2285
BlsI	GCNGC	7 cut(s)	80, 321, 451, 624, 1491, 1798, 2286
Bme1390I	CCNGG	3 cut(s)	1115, 1320, 1494
Bme18I	GGWCC	5 cut(s)	28, 71, 1003, 2011, 2092
BmgBI	CACGTC	1 cut(s)	488
BmgT120I	GGNCC	6 cut(s)	28, 58, 71, 1003, 2011, 2092
BmiI	GGNNCC	6 cut(s)	29, 59, 72, 251, 2012, 2262
BmrFI	CCNGG	3 cut(s)	1115, 1320, 1494
BmsI	GCATC	7 cut(s)	754, 772, 812, 1111, 1276, 1578, 1702
BoxI	GACNNNNGTC	1 cut(s)	69
BpiI	GAAGAC	3 cut(s)	675, 1245, 1395
BplI	GAGNNNNNCTC	2 cut(s)	67, 99
BpmI	CTGGAG	2 cut(s)	977, 2274
Bpu10I	CCTNAGC	3 cut(s)	389, 716, 2197
BpuEI	CTTGAG	3 cut(s)	173, 281, 741
Bsa29I	ATCGAT	1 cut(s)	1648
BsaAI	YACGTR	2 cut(s)	2098, 2300
BsaBI	GATNNNNATC	1 cut(s)	843
BsaHI	GRCGYC	1 cut(s)	2269
BsaI	GGTCTC	2 cut(s)	154, 1780
BsaJI	CCNNGG	2 cut(s)	1319, 2076
BsaXI	ACNNNNNCTCC	2 cut(s)	840, 870
Bsc4I	CCNNNNNNNGG	5 cut(s)	67, 814, 1998, 2144, 2283
Bse118I	RCCGGY	1 cut(s)	98
Bse1I	ACTGG	2 cut(s)	351, 960
Bse21I	CCTNAGG	1 cut(s)	809
Bse3DI	GCAATG	2 cut(s)	459, 609
Bse8I	GATNNNNATC	1 cut(s)	843
BseBI	CCWGG	3 cut(s)	1115, 1320, 1494
BseCI	ATCGAT	1 cut(s)	1648
BseDI	CCNNGG	2 cut(s)	1319, 2076
BseJI	GATNNNNATC	1 cut(s)	843
BseLI	CCNNNNNNNGG	5 cut(s)	67, 814, 1998, 2144, 2283
BseMI	GCAATG	2 cut(s)	459, 609
BseNI	ACTGG	2 cut(s)	351, 960
BseRI	GAGGAG	2 cut(s)	1037, 2163
BseSI	GKGCMC	1 cut(s)	11
BseXI	GCAGC	6 cut(s)	90, 306, 436, 634, 1501, 1783
BseYI	CCCAGC	1 cut(s)	1973
BsgI	GTGCAG	3 cut(s)	626, 884, 1782
Bsh1236I	CGCG	4 cut(s)	38, 327, 530, 681
BshFI	GGCC	3 cut(s)	60, 2033, 2287
BshVI	ATCGAT	1 cut(s)	1648
BsiHKAI	GWGCWC	4 cut(s)	285, 390, 528, 1417
BsiSI	CCGG	3 cut(s)	31, 86, 99
BslFI	GGGAC	1 cut(s)	2024
BslI	CCNNNNNNNGG	5 cut(s)	67, 814, 1998, 2144, 2283
BsmAI	GTCTC	9 cut(s)	154, 191, 197, 978, 1738, 1780, 2051, 2145, 2147
BsmFI	GGGAC	1 cut(s)	2024
BsnI	GGCC	3 cut(s)	60, 2033, 2287
Bso31I	GGTCTC	2 cut(s)	154, 1780
Bsp1286I	GDGCHC	5 cut(s)	11, 285, 390, 528, 1417
Bsp1407I	TGTACA	3 cut(s)	304, 588, 1405
BspACI	CCGC	3 cut(s)	111, 1232, 2284
BspANI	GGCC	3 cut(s)	60, 2033, 2287
BspDI	ATCGAT	1 cut(s)	1648
BspFNI	CGCG	4 cut(s)	38, 327, 530, 681
BspLI	GGNNCC	6 cut(s)	29, 59, 72, 251, 2012, 2262
BspMAI	CTGCAG	1 cut(s)	1916
BspMI	ACCTGC	3 cut(s)	1582, 1797, 2192
BspPI	GGATC	5 cut(s)	347, 852, 1468, 1493, 2183
BspTI	CTTAAG	1 cut(s)	1243
BspTNI	GGTCTC	2 cut(s)	154, 1780
BsrBI	CCGCTC	1 cut(s)	111
BsrDI	GCAATG	2 cut(s)	459, 609
BsrFI	RCCGGY	1 cut(s)	98
BsrGI	TGTACA	3 cut(s)	304, 588, 1405
BsrI	ACTGG	2 cut(s)	351, 960
BssAI	RCCGGY	1 cut(s)	98
BssECI	CCNNGG	2 cut(s)	1319, 2076
BssNI	GRCGYC	1 cut(s)	2269
BssSI	CACGAG	1 cut(s)	284
BssT1I	CCWWGG	1 cut(s)	2076
Bst2BI	CACGAG	1 cut(s)	284
Bst2UI	CCWGG	3 cut(s)	1115, 1320, 1494
Bst4CI	ACNGT	4 cut(s)	635, 661, 952, 2103
Bst6I	CTCTTC	3 cut(s)	426, 1566, 1927
BstACI	GRCGYC	1 cut(s)	2269
BstAFI	CTTAAG	1 cut(s)	1243
BstAUI	TGTACA	3 cut(s)	304, 588, 1405
BstBAI	YACGTR	2 cut(s)	2098, 2300
BstC8I	GCNNGC	4 cut(s)	36, 528, 616, 620
BstFNI	CGCG	4 cut(s)	38, 327, 530, 681
BstH2I	RGCGCY	1 cut(s)	84
BstHHI	GCGC	4 cut(s)	16, 83, 681, 1761
BstMAI	GTCTC	9 cut(s)	154, 191, 197, 978, 1738, 1780, 2051, 2145, 2147
BstMWI	GCNNNNNNNGC	2 cut(s)	95, 1321
BstNI	CCWGG	3 cut(s)	1115, 1320, 1494
BstNSI	RCATGY	2 cut(s)	724, 2284
BstPAI	GACNNNNGTC	1 cut(s)	69
BstSCI	CCNGG	3 cut(s)	1113, 1318, 1492
BstSFI	CTRYAG	3 cut(s)	657, 1812, 1912
BstSLI	GKGCMC	1 cut(s)	11
BstUI	CGCG	4 cut(s)	38, 327, 530, 681
BstV1I	GCAGC	6 cut(s)	90, 306, 436, 634, 1501, 1783
BstV2I	GAAGAC	3 cut(s)	675, 1245, 1395
BstX2I	RGATCY	2 cut(s)	339, 1498
BstXI	CCANNNNNNTGG	1 cut(s)	1575
BstYI	RGATCY	2 cut(s)	339, 1498
Bsu15I	ATCGAT	1 cut(s)	1648
Bsu36I	CCTNAGG	1 cut(s)	809
BsuRI	GGCC	3 cut(s)	60, 2033, 2287
BsuTUI	ATCGAT	1 cut(s)	1648
BtgZI	GCGATG	1 cut(s)	342
BtrI	CACGTC	1 cut(s)	488
BtsI	GCAGTG	2 cut(s)	9, 1761
BtsIMutI	CAGTG	7 cut(s)	9, 206, 575, 747, 1248, 1761, 1929
BveI	ACCTGC	3 cut(s)	1582, 1797, 2192
Cac8I	GCNNGC	4 cut(s)	36, 528, 616, 620
CfoI	GCGC	4 cut(s)	16, 83, 681, 1761
Cfr10I	RCCGGY	1 cut(s)	98
Cfr13I	GGNCC	6 cut(s)	28, 58, 71, 1003, 2011, 2092
ClaI	ATCGAT	1 cut(s)	1648
CseI	GACGC	1 cut(s)	152
Csp6I	GTAC	9 cut(s)	305, 589, 628, 892, 953, 1031, 1406, 1943, 2099
CspCI	CAANNNNNGTGG	2 cut(s)	363, 398
CviAII	CATG	7 cut(s)	67, 721, 1709, 1730, 2052, 2139, 2281
CviQI	GTAC	9 cut(s)	305, 589, 628, 892, 953, 1031, 1406, 1943, 2099
DraI	TTTAAA	1 cut(s)	1546
DraIII	CACNNNGTG	1 cut(s)	212
Eam1104I	CTCTTC	3 cut(s)	426, 1566, 1927
EarI	CTCTTC	3 cut(s)	426, 1566, 1927
EciI	GGCGGA	1 cut(s)	1247
Ecl136II	GAGCTC	1 cut(s)	388
Eco130I	CCWWGG	1 cut(s)	2076
Eco24I	GRGCYC	1 cut(s)	390
Eco31I	GGTCTC	2 cut(s)	154, 1780
Eco47I	GGWCC	5 cut(s)	28, 71, 1003, 2011, 2092
Eco47III	AGCGCT	1 cut(s)	82
Eco53kI	GAGCTC	1 cut(s)	388
Eco57I	CTGAAG	3 cut(s)	1272, 1641, 2117
Eco81I	CCTNAGG	1 cut(s)	809
EcoICRI	GAGCTC	1 cut(s)	388
EcoRI	GAATTC	2 cut(s)	370, 1127
EcoRII	CCWGG	3 cut(s)	1113, 1318, 1492
EcoT14I	CCWWGG	1 cut(s)	2076
EcoT38I	GRGCYC	1 cut(s)	390
ErhI	CCWWGG	1 cut(s)	2076
FaeI	CATG	7 cut(s)	70, 724, 1712, 1733, 2055, 2142, 2284
FaqI	GGGAC	1 cut(s)	2024
FatI	CATG	7 cut(s)	66, 720, 1708, 1729, 2051, 2138, 2280
Fnu4HI	GCNGC	7 cut(s)	79, 320, 450, 623, 1490, 1797, 2285
FriOI	GRGCYC	1 cut(s)	390
Fsp4HI	GCNGC	7 cut(s)	79, 320, 450, 623, 1490, 1797, 2285
FspAI	RTGCGCAY	1 cut(s)	1760
FspBI	CTAG	4 cut(s)	468, 483, 915, 1926
FspI	TGCGCA	2 cut(s)	15, 1760
GlaI	GCGC	4 cut(s)	15, 82, 680, 1760
GluI	GCNGC	7 cut(s)	79, 320, 450, 623, 1490, 1797, 2285
GsaI	CCCAGC	1 cut(s)	1977
GsuI	CTGGAG	2 cut(s)	977, 2274
HaeII	RGCGCY	1 cut(s)	84
HaeIII	GGCC	3 cut(s)	60, 2033, 2287
HapII	CCGG	3 cut(s)	31, 86, 99
HgaI	GACGC	1 cut(s)	152
HhaI	GCGC	4 cut(s)	16, 83, 681, 1761
Hin1I	GRCGYC	1 cut(s)	2269
Hin1II	CATG	7 cut(s)	70, 724, 1712, 1733, 2055, 2142, 2284
Hin6I	GCGC	4 cut(s)	14, 81, 679, 1759
HinP1I	GCGC	4 cut(s)	14, 81, 679, 1759
HincII	GTYRAC	2 cut(s)	142, 2004
HindII	GTYRAC	2 cut(s)	142, 2004
HindIII	AAGCTT	2 cut(s)	616, 2117
HinfI	GANTC	9 cut(s)	180, 458, 899, 1108, 1519, 1957, 2153, 2207, 2294
HpaII	CCGG	3 cut(s)	31, 86, 99
HphI	GGTGA	3 cut(s)	61, 848, 2216
Hpy166II	GTNNAC	7 cut(s)	52, 126, 142, 638, 1982, 2004, 2176
Hpy8I	GTNNAC	7 cut(s)	52, 126, 142, 638, 1982, 2004, 2176
Hpy99I	CGWCG	2 cut(s)	2066, 2271
HpyAV	CCTTC	8 cut(s)	166, 682, 994, 1070, 1136, 1247, 1252, 2080
HpyCH4III	ACNGT	4 cut(s)	635, 661, 952, 2103
HpyCH4IV	ACGT	6 cut(s)	487, 890, 2064, 2097, 2269, 2299
HpyF10VI	GCNNNNNNNGC	2 cut(s)	95, 1321
HpySE526I	ACGT	6 cut(s)	487, 890, 2064, 2097, 2269, 2299
Hsp92I	GRCGYC	1 cut(s)	2269
Hsp92II	CATG	7 cut(s)	70, 724, 1712, 1733, 2055, 2142, 2284
HspAI	GCGC	4 cut(s)	14, 81, 679, 1759
LmnI	GCTCC	2 cut(s)	88, 385
Lsp1109I	GCAGC	6 cut(s)	90, 306, 436, 634, 1501, 1783
LweI	GCATC	7 cut(s)	754, 772, 812, 1111, 1276, 1578, 1702
MaeI	CTAG	4 cut(s)	468, 483, 915, 1926
MaeII	ACGT	6 cut(s)	487, 890, 2064, 2097, 2269, 2299
MaeIII	GTNAC	2 cut(s)	980, 1101
MbiI	CCGCTC	1 cut(s)	111
MflI	RGATCY	2 cut(s)	339, 1498
MhlI	GDGCHC	5 cut(s)	11, 285, 390, 528, 1417
MluCI	AATT	9 cut(s)	19, 365, 370, 375, 419, 492, 508, 1127, 1539
MluI	ACGCGT	1 cut(s)	36
MlyI	GAGTC	2 cut(s)	1102, 2147
MmeI	TCCRAC	5 cut(s)	417, 1065, 1298, 1556, 1584
MroXI	GAANNNNTTC	1 cut(s)	1623
MseI	TTAA	7 cut(s)	222, 675, 725, 1244, 1545, 1884, 2057
MspA1I	CMGCKG	1 cut(s)	878
MspCI	CTTAAG	1 cut(s)	1243
MspI	CCGG	3 cut(s)	31, 86, 99
MspR9I	CCNGG	3 cut(s)	1115, 1320, 1494
MvaI	CCWGG	3 cut(s)	1115, 1320, 1494
MvnI	CGCG	4 cut(s)	38, 327, 530, 681
MwoI	GCNNNNNNNGC	2 cut(s)	95, 1321
NlaIII	CATG	7 cut(s)	70, 724, 1712, 1733, 2055, 2142, 2284
NlaIV	GGNNCC	6 cut(s)	29, 59, 72, 251, 2012, 2262
NmuCI	GTSAC	1 cut(s)	980
NsbI	TGCGCA	2 cut(s)	15, 1760
NspI	RCATGY	2 cut(s)	724, 2284
PaqCI	CACCTGC	2 cut(s)	1582, 2192
PdmI	GAANNNNTTC	1 cut(s)	1623
PfeI	GAWTC	7 cut(s)	180, 458, 899, 1519, 1957, 2207, 2294
PflMI	CCANNNNNTGG	1 cut(s)	67
PkrI	GCNGC	7 cut(s)	80, 321, 451, 624, 1491, 1798, 2286
PleI	GAGTC	2 cut(s)	1102, 2147
PpsI	GAGTC	2 cut(s)	1102, 2147
Ppu21I	YACGTR	2 cut(s)	2098, 2300
PshAI	GACNNNNGTC	1 cut(s)	69
PsiI	TTATAA	1 cut(s)	1428
Psp124BI	GAGCTC	1 cut(s)	390
Psp6I	CCWGG	3 cut(s)	1113, 1318, 1492
PspFI	CCCAGC	1 cut(s)	1973
PspGI	CCWGG	3 cut(s)	1113, 1318, 1492
PspN4I	GGNNCC	6 cut(s)	29, 59, 72, 251, 2012, 2262
PspPI	GGNCC	6 cut(s)	28, 58, 71, 1003, 2011, 2092
PsrI	GAACNNNNNNTAC	4 cut(s)	85, 117, 1926, 1958
PstI	CTGCAG	1 cut(s)	1916
PsuI	RGATCY	2 cut(s)	339, 1498
PvuII	CAGCTG	1 cut(s)	878
RsaI	GTAC	9 cut(s)	306, 590, 629, 893, 954, 1032, 1407, 1944, 2100
RsaNI	GTAC	9 cut(s)	305, 589, 628, 892, 953, 1031, 1406, 1943, 2099
SacI	GAGCTC	1 cut(s)	390
SaqAI	TTAA	7 cut(s)	222, 675, 725, 1244, 1545, 1884, 2057
SatI	GCNGC	7 cut(s)	79, 320, 450, 623, 1490, 1797, 2285
Sau96I	GGNCC	6 cut(s)	28, 58, 71, 1003, 2011, 2092
SchI	GAGTC	2 cut(s)	1102, 2147
ScrFI	CCNGG	3 cut(s)	1115, 1320, 1494
SduI	GDGCHC	5 cut(s)	11, 285, 390, 528, 1417
SfaNI	GCATC	7 cut(s)	754, 772, 812, 1111, 1276, 1578, 1702
SfcI	CTRYAG	3 cut(s)	657, 1812, 1912
SinI	GGWCC	5 cut(s)	28, 71, 1003, 2011, 2092
SmlI	CTYRAG	4 cut(s)	188, 260, 756, 1243
SmoI	CTYRAG	4 cut(s)	188, 260, 756, 1243
Sse9I	AATT	9 cut(s)	19, 365, 370, 375, 419, 492, 508, 1127, 1539
SsiI	CCGC	3 cut(s)	111, 1232, 2284
SspI	AATATT	1 cut(s)	2070
SspMI	CTAG	4 cut(s)	468, 483, 915, 1926
SstI	GAGCTC	1 cut(s)	390
StyD4I	CCNGG	3 cut(s)	1113, 1318, 1492
StyI	CCWWGG	1 cut(s)	2076
TaaI	ACNGT	4 cut(s)	635, 661, 952, 2103
TaiI	ACGT	6 cut(s)	490, 893, 2067, 2100, 2272, 2302
TaqI	TCGA	4 cut(s)	847, 1213, 1598, 1648
TaqII	GACCGA	1 cut(s)	2028
TasI	AATT	9 cut(s)	19, 365, 370, 375, 419, 492, 508, 1127, 1539
TatI	WGTACW	6 cut(s)	304, 588, 627, 1030, 1405, 1942
TauI	GCSGC	1 cut(s)	2287
TfiI	GAWTC	7 cut(s)	180, 458, 899, 1519, 1957, 2207, 2294
Tru1I	TTAA	7 cut(s)	222, 675, 725, 1244, 1545, 1884, 2057
Tru9I	TTAA	7 cut(s)	222, 675, 725, 1244, 1545, 1884, 2057
TscAI	CASTG	7 cut(s)	16, 213, 582, 747, 1255, 1768, 1936
TseFI	GTSAC	1 cut(s)	980
TseI	GCWGC	6 cut(s)	78, 319, 449, 622, 1489, 1796
Tsp45I	GTSAC	1 cut(s)	980
TspDTI	ATGAA	7 cut(s)	345, 810, 1089, 1251, 1518, 1632, 1844
TspGWI	ACGGA	1 cut(s)	1190
TspRI	CASTG	7 cut(s)	16, 213, 582, 747, 1255, 1768, 1936
Van91I	CCANNNNNTGG	1 cut(s)	67
Vha464I	CTTAAG	1 cut(s)	1243
VpaK11BI	GGWCC	5 cut(s)	28, 71, 1003, 2011, 2092
XapI	RAATTY	5 cut(s)	370, 419, 508, 1127, 1539
XceI	RCATGY	2 cut(s)	724, 2284
XmnI	GAANNNNTTC	1 cut(s)	1623
XspI	CTAG	4 cut(s)	468, 483, 915, 1926
ZraI	GACGTC	1 cut(s)	2270

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.