Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006514

Physical Location & Seq

Genomic Coordinates Reverse (-)

29202083 .. 29207278

Gene Structure 5196 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1212900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2280 bp

ATGGTGGGCCCCGCGAAAAATGGGAGGGTCCGGCACGCGTTCTCGGTGGTGAACGGGGGCCAAGAGTTGGGTCCGAGCAGCGCTCCGGTGAGTAATGCCGGTTCGGAGAGCGGTGGTATTGAGTTTACCAGAGAGGATGTTGACGCATTGTTGAATGAGAGACCCAAAAGGAAGGACAGGTTCAGTCTCAAGGAAAAATGTGAGACTATGAGTGAGTATATTAAAAGGCTGAAACTTTGCATAAAGTGGTTTCAAGAGCTTGAAGAAAGCTACTTAATTGAGCGCAAGAAGTTAGAGAATGATGTGGAAGTTTCAGAGCGGCAGCGGGATGAAATGGGGATCTTACTGAAAAACAAGGAAGAAGAACTGAATTCGATTATTGCGGAGCTGAGGAAAAACTATGCTTCCTTACAAGAGAAATTTACAAAAGAAGAGTTGGATAAGATGGCTGCAATGGAATCTCTCGCGAGGGAAAGAGAGACTAGACTTGAAATTGAGCGATCACAAAATTCCCTATCGGATGAGCATGGGAGAGTTCAAAGAGAGCTTTCAAGTGCTACGCAGAAGATAACTTCACTGAATGATATGTACAAACGATTGCAAGAATACATTGCAAGCTTGCAACAGTACAATAGTAAACTGCACAACGACCTCTCTACAGTTGAGGAAGACTTTAAGCGCGTAGAGAAGGAAAAAGTTTGTATGGTGGAGAACCTGAGCATGCTAAGGGGTCAACTTTCAGTGTCCAAACAAACTCAAGATGATGCTATAAAGCAGAGGGATGCTTTGGTAAATGAAGTTGCATCCCTTAGGATGGAGTTGCAGCAAGTGAGAGATGATCGGGATCGTCATAAACTCCAAGTGCAGACCCTAACAGCTGAATATACAACATACAAAGAATCCACCGAGAAATCTAGTTTTGAGGTGGATAACCTCAAATCAAAATATACCGAACTGGAGGTAAGATGTCTGTCTCAGACTGACCAGATAAAGACATTGAAGGACCAACTAATGATTGCAGAGGAGAAAGTACAGATGTCCAACTTAACTACACTGGAAACAAGAACTGACTATGAAGGGCAAAAGAAACTTATAAGTGAGTTACAAACTCGCCTGGCAGATGCAGAATTCAAACTTATTGAAGGAGAAATGCTGCGTAAAAAGTTGCACAACACTATCTTGGAACTAAAAGGGAACATCCGTGTATTCTGTCGAGTGCGACCTTTGTTGGCGGATGAAGACGTAAGCACTGAAGGGAAGGTTATCTCTTATCCAACATCAATGGATGCTCTTGGACGGGGCATTGATTTAGTGCAACCTGGGCAAAAACATTCTTTCACATTTGACAAAGTTTTTATGCCTGAAATATCACAAGAAGATGTGTTTGAAGACATTTCACAGCTCGTACAAAGTGCTCTTGATGGTTATAAGGTTTGCATTTTTGCCTATGGTCAGACAGGATCAGGGAAAACCTATACCATGATGGGTAAGCCAGGAGATCAAGACCAAAAAGGGTTGGTTCCTCGTTCATTAGAACAAATTTTTAAAACCATACAGTCTCTTCAACCACAAGGTTGGAAATATGAAATGCAGGTGTCGATGTTGGAAATATACAATGAAACTATTCGTGATCTGTTAGCACCCAATCGATCATCTTCAGATATGTTGCGAGAAAATGGTATTGGTGGAAAGCAATATACAATCAAACACGATGCAAATGGGAACACTCATGTCTCTGATCTTACCATTGTTGATGTGTGCACTGCTAAAGAGGTCTCATATCTCTTAGAACGGGCTGAACATAGCAGGTCTGTAGGCAAAACTCAAATGAACGAGCAATCTTCAAGAAGCCATTTTGTTTTCACACTACGGATATCTGGTGTTAATGAGAGCACTGAACAACAAGTACAAGGTGTTCTGAATCTTATTGATCTTGCTGGGAGTGAACGACTTTCCAAGAGTGGGTCAACGGGAGACCGACTGAAAGAAACTCAGGCCATCAACAAAAGTCTATCATCTTTAAGCGACGTGATATTTGCCTTGGCAAAGAAGGAGGACCACGTACCATTTAGGAACTCAAAGCTTACATATCTTCTTCAGCCATGTCTAGGAGGAGACTCAAAGACATTGATGTTCGTAAACATTTCTCCCGAGCCCTCCTCAGCAGGTGAATCACTCTGTTCCCTTCGTTTTGCCTCACGGGTAAACGCTTGCGATATTGGGGTTCCACGGCGTCAGACCAGCATGCGGCCTCCAGATTCACGTATGAGCTATGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008017 GO:0008092 GO:0008150 GO:0009987 GO:0015630 GO:0015631 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin Microtub_bd

Protein Analysis

760

Amino Acids

86.2

Weight (kDa)

5.9

Isoelectric Point (pI)

47.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	381 - 546	1.5e-54	Microtubule binding
Kinesin	PF00225	405 - 737	1.6e-113	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006514)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27180 AT4G27180 AT5G54670 AT5G54670 AT5G54670
fragaria_vesca	FvH4_3g21681 FvH4_3g21681 FvH4_3g21681 FvH4_3g21681 FvH4_3g21681
malus_domestica	MD03G1212900.v1.1 MD11G1228000.v1.1
prunus_persica	Prupe.4G201200_v2.0.a1 Prupe.4G201200_v2.0.a1
pyrus_communis	pycom03g16170 pycom11g20070
rosa_chinensis	RchiOBHm_Chr5g0037181
rosa_laevigata	RLG00000033756
rosa_multiflora	Rmu_sc0030361.1_g000001
rosa_roxburghii	Rroxscaffold_1G00043800
rosa_rugosa	Rorug05G0161400
rosa_samantha	Rh5AG253000 Rh5BG254900 Rh5CG287600 Rh5DG263400
rosa_wichuraiana	Rw5G023370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1094, 1428
AarI	CACCTGC	2 cut(s)	1582, 2156
Acc36I	ACCTGC	3 cut(s)	1582, 1797, 2156
AccB7I	CCANNNNNTGG	1 cut(s)	67
AccBSI	CCGCTC	2 cut(s)	111, 319
AccII	CGCG	4 cut(s)	14, 38, 467, 681
AciI	CCGC	7 cut(s)	12, 111, 319, 325, 383, 1232, 2248
AclWI	GGATC	3 cut(s)	347, 852, 1468
AcsI	RAATTY	5 cut(s)	370, 419, 508, 1127, 1539
AcuI	CTGAAG	3 cut(s)	1272, 1641, 2081
AcyI	GRCGYC	1 cut(s)	2233
AfaI	GTAC	6 cut(s)	590, 629, 1032, 1407, 1908, 2064
AfeI	AGCGCT	1 cut(s)	82
AfiI	CCNNNNNNNGG	5 cut(s)	67, 814, 1962, 2108, 2247
AflIII	ACRYGT	1 cut(s)	36
AjiI	CACGTC	1 cut(s)	2029
AjnI	CCWGG	3 cut(s)	1113, 1318, 1492
AjuI	GAANNNNNNNTTGG	2 cut(s)	1665, 1697
AluBI	AGCT	9 cut(s)	259, 270, 388, 547, 618, 878, 1402, 2083, 2271
AluI	AGCT	9 cut(s)	259, 270, 388, 547, 618, 878, 1402, 2083, 2271
Alw21I	GWGCWC	3 cut(s)	1417, 1763, 1895
Alw44I	GTGCAC	1 cut(s)	1759
AlwI	GGATC	3 cut(s)	347, 852, 1468
Ama87I	CYCGRG	1 cut(s)	2150
Aor51HI	AGCGCT	1 cut(s)	82
AoxI	GGCC	4 cut(s)	7, 58, 1995, 2249
ApaI	GGGCCC	1 cut(s)	11
ApaLI	GTGCAC	1 cut(s)	1759
ApeKI	GCWGC	5 cut(s)	78, 322, 449, 823, 1153
ApoI	RAATTY	5 cut(s)	370, 419, 508, 1127, 1539
ArsI	GACNNNNNNTTYG	2 cut(s)	2018, 2050
Asp700I	GAANNNNTTC	1 cut(s)	1623
AspLEI	GCGC	3 cut(s)	83, 285, 681
AspS9I	GGNCC	7 cut(s)	7, 8, 28, 58, 71, 1003, 2056
AsuHPI	GGTGA	3 cut(s)	61, 100, 2180
AvaI	CYCGRG	1 cut(s)	2150
AvaII	GGWCC	4 cut(s)	28, 71, 1003, 2056
AxyI	CCTNAGG	1 cut(s)	809
BaeGI	GKGCMC	2 cut(s)	11, 1763
BanII	GRGCYC	2 cut(s)	11, 2157
BbsI	GAAGAC	3 cut(s)	675, 1245, 1395
Bbv12I	GWGCWC	3 cut(s)	1417, 1763, 1895
BbvCI	CCTCAGC	2 cut(s)	389, 2161
BbvI	GCAGC	5 cut(s)	90, 334, 436, 835, 1140
BccI	CCATC	5 cut(s)	439, 808, 1415, 1477, 2006
BceAI	ACGGC	1 cut(s)	2246
BciT130I	CCWGG	3 cut(s)	1115, 1320, 1494
BfaI	CTAG	3 cut(s)	483, 915, 2108
BfmI	CTRYAG	2 cut(s)	657, 1812
BfoI	RGCGCY	1 cut(s)	84
BfuAI	ACCTGC	3 cut(s)	1582, 1797, 2156
BisI	GCNGC	7 cut(s)	79, 320, 323, 450, 824, 1154, 2249
BlsI	GCNGC	7 cut(s)	80, 321, 324, 451, 825, 1155, 2250
Bme1390I	CCNGG	3 cut(s)	1115, 1320, 1494
Bme18I	GGWCC	4 cut(s)	28, 71, 1003, 2056
BmeT110I	CYCGRG	1 cut(s)	2150
BmgBI	CACGTC	1 cut(s)	2029
BmgT120I	GGNCC	7 cut(s)	7, 8, 28, 58, 71, 1003, 2056
BmiI	GGNNCC	7 cut(s)	9, 10, 29, 59, 72, 1521, 2226
BmrFI	CCNGG	3 cut(s)	1115, 1320, 1494
BmsI	GCATC	6 cut(s)	754, 772, 812, 1111, 1276, 1702
BpiI	GAAGAC	3 cut(s)	675, 1245, 1395
BplI	GAGNNNNNCTC	4 cut(s)	67, 99, 2144, 2176
BpmI	CTGGAG	2 cut(s)	977, 2238
Bpu10I	CCTNAGC	4 cut(s)	389, 716, 725, 2161
BpuEI	CTTGAG	2 cut(s)	173, 741
Bsa29I	ATCGAT	1 cut(s)	1648
BsaAI	YACGTR	2 cut(s)	2062, 2264
BsaBI	GATNNNNATC	1 cut(s)	843
BsaHI	GRCGYC	1 cut(s)	2233
BsaI	GGTCTC	3 cut(s)	154, 1780, 1968
BsaJI	CCNNGG	3 cut(s)	1319, 2040, 2228
BsaWI	WCCGGW	1 cut(s)	85
Bsc4I	CCNNNNNNNGG	5 cut(s)	67, 814, 1962, 2108, 2247
Bse118I	RCCGGY	1 cut(s)	98
Bse1I	ACTGG	2 cut(s)	960, 1059
Bse21I	CCTNAGG	1 cut(s)	809
Bse3DI	GCAATG	2 cut(s)	459, 609
Bse8I	GATNNNNATC	1 cut(s)	843
BseBI	CCWGG	3 cut(s)	1115, 1320, 1494
BseCI	ATCGAT	1 cut(s)	1648
BseDI	CCNNGG	3 cut(s)	1319, 2040, 2228
BseGI	GGATG	9 cut(s)	142, 334, 526, 787, 803, 819, 1197, 1240, 1291
BseJI	GATNNNNATC	1 cut(s)	843
BseLI	CCNNNNNNNGG	5 cut(s)	67, 814, 1962, 2108, 2247
BseMI	GCAATG	2 cut(s)	459, 609
BseMII	CTCAG	5 cut(s)	380, 707, 989, 2006, 2175
BseNI	ACTGG	2 cut(s)	960, 1059
BseRI	GAGGAG	3 cut(s)	1037, 2127, 2149
BseSI	GKGCMC	2 cut(s)	11, 1763
BseXI	GCAGC	5 cut(s)	90, 334, 436, 835, 1140
BseYI	CCCAGC	1 cut(s)	1937
BsgI	GTGCAG	2 cut(s)	626, 884
Bsh1236I	CGCG	4 cut(s)	14, 38, 467, 681
BshFI	GGCC	4 cut(s)	9, 60, 1997, 2251
BshVI	ATCGAT	1 cut(s)	1648
BsiHKAI	GWGCWC	3 cut(s)	1417, 1763, 1895
BsiHKCI	CYCGRG	1 cut(s)	2150
BsiSI	CCGG	3 cut(s)	31, 86, 99
BslI	CCNNNNNNNGG	5 cut(s)	67, 814, 1962, 2108, 2247
BsnI	GGCC	4 cut(s)	9, 60, 1997, 2251
Bso31I	GGTCTC	3 cut(s)	154, 1780, 1968
BsoBI	CYCGRG	1 cut(s)	2150
Bsp120I	GGGCCC	1 cut(s)	7
Bsp1286I	GDGCHC	5 cut(s)	11, 1417, 1763, 1895, 2157
Bsp1407I	TGTACA	1 cut(s)	588
Bsp68I	TCGCGA	1 cut(s)	467
BspACI	CCGC	7 cut(s)	12, 111, 319, 325, 383, 1232, 2248
BspANI	GGCC	4 cut(s)	9, 60, 1997, 2251
BspCNI	CTCAG	5 cut(s)	381, 708, 988, 2005, 2174
BspDI	ATCGAT	1 cut(s)	1648
BspFNI	CGCG	4 cut(s)	14, 38, 467, 681
BspLI	GGNNCC	7 cut(s)	9, 10, 29, 59, 72, 1521, 2226
BspMI	ACCTGC	3 cut(s)	1582, 1797, 2156
BspPI	GGATC	3 cut(s)	347, 852, 1468
BspTNI	GGTCTC	3 cut(s)	154, 1780, 1968
BsrBI	CCGCTC	2 cut(s)	111, 319
BsrDI	GCAATG	2 cut(s)	459, 609
BsrFI	RCCGGY	1 cut(s)	98
BsrGI	TGTACA	1 cut(s)	588
BsrI	ACTGG	2 cut(s)	960, 1059
BssAI	RCCGGY	1 cut(s)	98
BssECI	CCNNGG	3 cut(s)	1319, 2040, 2228
BssNI	GRCGYC	1 cut(s)	2233
BssT1I	CCWWGG	1 cut(s)	2040
Bst2UI	CCWGG	3 cut(s)	1115, 1320, 1494
Bst4CI	ACNGT	3 cut(s)	627, 661, 1557
Bst6I	CTCTTC	2 cut(s)	426, 1566
BstACI	GRCGYC	1 cut(s)	2233
BstAUI	TGTACA	1 cut(s)	588
BstBAI	YACGTR	2 cut(s)	2062, 2264
BstC8I	GCNNGC	6 cut(s)	36, 616, 620, 722, 2212, 2246
BstDEI	CTNAG	8 cut(s)	389, 716, 725, 809, 975, 1786, 1992, 2161
BstDSI	CCRYGG	1 cut(s)	2228
BstF5I	GGATG	9 cut(s)	142, 334, 526, 787, 803, 819, 1197, 1240, 1291
BstFNI	CGCG	4 cut(s)	14, 38, 467, 681
BstH2I	RGCGCY	1 cut(s)	84
BstHHI	GCGC	3 cut(s)	83, 285, 681
BstMWI	GCNNNNNNNGC	1 cut(s)	1321
BstNI	CCWGG	3 cut(s)	1115, 1320, 1494
BstNSI	RCATGY	2 cut(s)	724, 2248
BstSCI	CCNGG	3 cut(s)	1113, 1318, 1492
BstSFI	CTRYAG	2 cut(s)	657, 1812
BstSLI	GKGCMC	2 cut(s)	11, 1763
BstUI	CGCG	4 cut(s)	14, 38, 467, 681
BstV1I	GCAGC	5 cut(s)	90, 334, 436, 835, 1140
BstV2I	GAAGAC	3 cut(s)	675, 1245, 1395
BstX2I	RGATCY	1 cut(s)	339
BstXI	CCANNNNNNTGG	1 cut(s)	1575
BstYI	RGATCY	1 cut(s)	339
Bsu15I	ATCGAT	1 cut(s)	1648
Bsu36I	CCTNAGG	1 cut(s)	809
BsuRI	GGCC	4 cut(s)	9, 60, 1997, 2251
BsuTUI	ATCGAT	1 cut(s)	1648
BtgI	CCRYGG	1 cut(s)	2228
BtrI	CACGTC	1 cut(s)	2029
BtsCI	GGATG	9 cut(s)	142, 334, 526, 787, 803, 819, 1197, 1240, 1291
BtsI	GCAGTG	1 cut(s)	1761
BtsIMutI	CAGTG	6 cut(s)	575, 747, 1052, 1248, 1761, 1893
BtuMI	TCGCGA	1 cut(s)	467
BveI	ACCTGC	3 cut(s)	1582, 1797, 2156
Cac8I	GCNNGC	6 cut(s)	36, 616, 620, 722, 2212, 2246
CfoI	GCGC	3 cut(s)	83, 285, 681
Cfr10I	RCCGGY	1 cut(s)	98
Cfr13I	GGNCC	7 cut(s)	7, 8, 28, 58, 71, 1003, 2056
ClaI	ATCGAT	1 cut(s)	1648
CseI	GACGC	2 cut(s)	152, 2222
Csp6I	GTAC	6 cut(s)	589, 628, 1031, 1406, 1907, 2063
CviAII	CATG	6 cut(s)	527, 721, 1480, 1730, 2103, 2245
CviQI	GTAC	6 cut(s)	589, 628, 1031, 1406, 1907, 2063
DdeI	CTNAG	8 cut(s)	389, 716, 725, 809, 975, 1786, 1992, 2161
DraI	TTTAAA	1 cut(s)	1546
Eam1104I	CTCTTC	2 cut(s)	426, 1566
EarI	CTCTTC	2 cut(s)	426, 1566
EciI	GGCGGA	1 cut(s)	1247
Eco130I	CCWWGG	1 cut(s)	2040
Eco24I	GRGCYC	2 cut(s)	11, 2157
Eco31I	GGTCTC	3 cut(s)	154, 1780, 1968
Eco32I	GATATC	1 cut(s)	1875
Eco47I	GGWCC	4 cut(s)	28, 71, 1003, 2056
Eco47III	AGCGCT	1 cut(s)	82
Eco57I	CTGAAG	3 cut(s)	1272, 1641, 2081
Eco81I	CCTNAGG	1 cut(s)	809
Eco88I	CYCGRG	1 cut(s)	2150
EcoO109I	RGGNCCY	1 cut(s)	8
EcoRI	GAATTC	2 cut(s)	370, 1127
EcoRII	CCWGG	3 cut(s)	1113, 1318, 1492
EcoRV	GATATC	1 cut(s)	1875
EcoT14I	CCWWGG	1 cut(s)	2040
EcoT38I	GRGCYC	2 cut(s)	11, 2157
ErhI	CCWWGG	1 cut(s)	2040
FaeI	CATG	6 cut(s)	530, 724, 1483, 1733, 2106, 2248
FatI	CATG	6 cut(s)	526, 720, 1479, 1729, 2102, 2244
FauI	CCCGC	2 cut(s)	19, 318
Fnu4HI	GCNGC	7 cut(s)	79, 320, 323, 450, 824, 1154, 2249
FokI	GGATG	9 cut(s)	149, 341, 533, 790, 794, 826, 1184, 1247, 1298
FriOI	GRGCYC	2 cut(s)	11, 2157
Fsp4HI	GCNGC	7 cut(s)	79, 320, 323, 450, 824, 1154, 2249
FspBI	CTAG	3 cut(s)	483, 915, 2108
GlaI	GCGC	3 cut(s)	82, 284, 680
GluI	GCNGC	7 cut(s)	79, 320, 323, 450, 824, 1154, 2249
GsaI	CCCAGC	1 cut(s)	1941
GsuI	CTGGAG	2 cut(s)	977, 2238
HaeII	RGCGCY	1 cut(s)	84
HaeIII	GGCC	4 cut(s)	9, 60, 1997, 2251
HapII	CCGG	3 cut(s)	31, 86, 99
HgaI	GACGC	2 cut(s)	152, 2222
HhaI	GCGC	3 cut(s)	83, 285, 681
Hin1I	GRCGYC	1 cut(s)	2233
Hin1II	CATG	6 cut(s)	530, 724, 1483, 1733, 2106, 2248
Hin6I	GCGC	3 cut(s)	81, 283, 679
HinP1I	GCGC	3 cut(s)	81, 283, 679
HincII	GTYRAC	3 cut(s)	142, 734, 1968
HindII	GTYRAC	3 cut(s)	142, 734, 1968
HindIII	AAGCTT	2 cut(s)	616, 2081
HinfI	GANTC	6 cut(s)	458, 899, 1921, 2117, 2171, 2258
HpaII	CCGG	3 cut(s)	31, 86, 99
HphI	GGTGA	3 cut(s)	61, 100, 2180
Hpy99I	CGWCG	1 cut(s)	2030
HpyAV	CCTTC	9 cut(s)	166, 682, 994, 1070, 1136, 1247, 1252, 2044, 2195
HpyCH4III	ACNGT	3 cut(s)	627, 661, 1557
HpyCH4IV	ACGT	4 cut(s)	1242, 2028, 2061, 2263
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1321
HpyF3I	CTNAG	8 cut(s)	389, 716, 725, 809, 975, 1786, 1992, 2161
HpySE526I	ACGT	4 cut(s)	1242, 2028, 2061, 2263
Hsp92I	GRCGYC	1 cut(s)	2233
Hsp92II	CATG	6 cut(s)	530, 724, 1483, 1733, 2106, 2248
HspAI	GCGC	3 cut(s)	81, 283, 679
LmnI	GCTCC	2 cut(s)	88, 385
Lsp1109I	GCAGC	5 cut(s)	90, 334, 436, 835, 1140
LweI	GCATC	6 cut(s)	754, 772, 812, 1111, 1276, 1702
MaeI	CTAG	3 cut(s)	483, 915, 2108
MaeII	ACGT	4 cut(s)	1242, 2028, 2061, 2263
MaeIII	GTNAC	1 cut(s)	1101
MbiI	CCGCTC	2 cut(s)	111, 319
MflI	RGATCY	1 cut(s)	339
MhlI	GDGCHC	5 cut(s)	11, 1417, 1763, 1895, 2157
MluCI	AATT	7 cut(s)	276, 370, 419, 492, 508, 1127, 1539
MluI	ACGCGT	1 cut(s)	36
MlyI	GAGTC	1 cut(s)	2111
MmeI	TCCRAC	5 cut(s)	417, 1065, 1298, 1556, 1584
MroXI	GAANNNNTTC	1 cut(s)	1623
MseI	TTAA	7 cut(s)	222, 275, 675, 1046, 1545, 1884, 2021
MspA1I	CMGCKG	2 cut(s)	325, 878
MspI	CCGG	3 cut(s)	31, 86, 99
MspR9I	CCNGG	3 cut(s)	1115, 1320, 1494
MvaI	CCWGG	3 cut(s)	1115, 1320, 1494
MvnI	CGCG	4 cut(s)	14, 38, 467, 681
MwoI	GCNNNNNNNGC	1 cut(s)	1321
NlaIII	CATG	6 cut(s)	530, 724, 1483, 1733, 2106, 2248
NlaIV	GGNNCC	7 cut(s)	9, 10, 29, 59, 72, 1521, 2226
NruI	TCGCGA	1 cut(s)	467
NspI	RCATGY	2 cut(s)	724, 2248
PaeI	GCATGC	2 cut(s)	724, 2248
PaqCI	CACCTGC	2 cut(s)	1582, 2156
PdmI	GAANNNNTTC	1 cut(s)	1623
PfeI	GAWTC	5 cut(s)	458, 899, 1921, 2171, 2258
PflMI	CCANNNNNTGG	1 cut(s)	67
PkrI	GCNGC	7 cut(s)	80, 321, 324, 451, 825, 1155, 2250
PleI	GAGTC	1 cut(s)	2111
PpsI	GAGTC	1 cut(s)	2111
Ppu21I	YACGTR	2 cut(s)	2062, 2264
PsiI	TTATAA	2 cut(s)	1094, 1428
Psp6I	CCWGG	3 cut(s)	1113, 1318, 1492
PspFI	CCCAGC	1 cut(s)	1937
PspGI	CCWGG	3 cut(s)	1113, 1318, 1492
PspN4I	GGNNCC	7 cut(s)	9, 10, 29, 59, 72, 1521, 2226
PspOMI	GGGCCC	1 cut(s)	7
PspPI	GGNCC	7 cut(s)	7, 8, 28, 58, 71, 1003, 2056
PsrI	GAACNNNNNNTAC	6 cut(s)	85, 117, 1890, 1899, 1922, 1931
PsuI	RGATCY	1 cut(s)	339
PvuII	CAGCTG	1 cut(s)	878
RruI	TCGCGA	1 cut(s)	467
RsaI	GTAC	6 cut(s)	590, 629, 1032, 1407, 1908, 2064
RsaNI	GTAC	6 cut(s)	589, 628, 1031, 1406, 1907, 2063
SaqAI	TTAA	7 cut(s)	222, 275, 675, 1046, 1545, 1884, 2021
SatI	GCNGC	7 cut(s)	79, 320, 323, 450, 824, 1154, 2249
Sau96I	GGNCC	7 cut(s)	7, 8, 28, 58, 71, 1003, 2056
SchI	GAGTC	1 cut(s)	2111
ScrFI	CCNGG	3 cut(s)	1115, 1320, 1494
SduI	GDGCHC	5 cut(s)	11, 1417, 1763, 1895, 2157
SfaNI	GCATC	6 cut(s)	754, 772, 812, 1111, 1276, 1702
SfcI	CTRYAG	2 cut(s)	657, 1812
SinI	GGWCC	4 cut(s)	28, 71, 1003, 2056
SmlI	CTYRAG	2 cut(s)	188, 756
SmoI	CTYRAG	2 cut(s)	188, 756
SphI	GCATGC	2 cut(s)	724, 2248
Sse9I	AATT	7 cut(s)	276, 370, 419, 492, 508, 1127, 1539
SsiI	CCGC	7 cut(s)	12, 111, 319, 325, 383, 1232, 2248
SspMI	CTAG	3 cut(s)	483, 915, 2108
StyD4I	CCNGG	3 cut(s)	1113, 1318, 1492
StyI	CCWWGG	1 cut(s)	2040
TaaI	ACNGT	3 cut(s)	627, 661, 1557
TaiI	ACGT	4 cut(s)	1245, 2031, 2064, 2266
TaqI	TCGA	4 cut(s)	374, 1213, 1598, 1648
TaqII	GACCGA	1 cut(s)	1992
TasI	AATT	7 cut(s)	276, 370, 419, 492, 508, 1127, 1539
TatI	WGTACW	4 cut(s)	588, 627, 1030, 1906
TauI	GCSGC	2 cut(s)	322, 2251
TfiI	GAWTC	5 cut(s)	458, 899, 1921, 2171, 2258
Tru1I	TTAA	7 cut(s)	222, 275, 675, 1046, 1545, 1884, 2021
Tru9I	TTAA	7 cut(s)	222, 275, 675, 1046, 1545, 1884, 2021
TscAI	CASTG	6 cut(s)	582, 747, 1059, 1255, 1768, 1900
TseI	GCWGC	5 cut(s)	78, 322, 449, 823, 1153
TspDTI	ATGAA	8 cut(s)	345, 810, 1089, 1251, 1518, 1599, 1632, 1844
TspGWI	ACGGA	2 cut(s)	1190, 1885
TspRI	CASTG	6 cut(s)	582, 747, 1059, 1255, 1768, 1900
Van91I	CCANNNNNTGG	1 cut(s)	67
VneI	GTGCAC	1 cut(s)	1759
VpaK11BI	GGWCC	4 cut(s)	28, 71, 1003, 2056
XapI	RAATTY	5 cut(s)	370, 419, 508, 1127, 1539
XceI	RCATGY	2 cut(s)	724, 2248
XmnI	GAANNNNTTC	1 cut(s)	1623
XspI	CTAG	3 cut(s)	483, 915, 2108

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.