AT4G29040

26S proteasome regulatory subunit 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004988
Physical Location & Seq
Forward (+)
14312283 .. 14314600
2318 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G29040.1

Sequence Viewer

Length: 1332 bp
ATGGGACAAGGACCATCGGGAGGATTGAATCGGCAAGGAGATCGGAAACCTGATGGTGGAGATAAGAAAGAGAAGAAGTTTGAGCCGGCGGCACCACCGGCTCGTGTAGGGAGGAAGCAAAGGAAGCAGAAAGGGCCAGAGGCGGCGGCGAGACTTCCGACGGTGACTCCGTCTACTAAGTGTAAGCTCCGGTTACTGAAATTGGAACGGATCAAGGATTATTTGTTGATGGAGGAAGAGTTTGTGGCGAATCAAGAGAGGTTGAAACCTCAGGAAGAGAAAGCTGAGGAAGATAGATCTAAGGTTGATGATCTTCGTGGTACACCTATGAGTGTTGGGAATTTGGAAGAGTTGATTGATGAGAATCACGCTATTGTTTCTTCTTCTGTTGGACCTGAGTACTACGTTGGGATTTTGTCCTTCGTTGATAAAGATCAGCTTGAACCTGGATGTTCGATTTTGATGCATAATAAGGTTCTCTCTGTTGTTGGGATTCTACAAGATGAAGTGGATCCAATGGTGTCTGTGATGAAAGTTGAGAAAGCTCCTTTGGAGTCATATGCTGATATTGGAGGTTTAGAAGCTCAGATTCAGGAGATTAAGGAAGCTGTTGAGCTTCCTTTGACTCATCCTGAGTTGTATGAAGATATTGGTATTAAACCACCAAAGGGTGTAATTTTGTATGGTGAGCCTGGAACTGGGAAGACATTGCTTGCTAAGGCGGTTGCGAATTCAACATCAGCTACTTTCTTGAGAGTTGTTGGTAGTGAGCTGATTCAGAAGTATTTGGGAGATGGTCCTAAGCTTGTGAGAGAACTTTTCAGAGTTGCTGATGACCTTTCACCATCAATCGTCTTCATAGATGAGATTGATGCTGTTGGTACCAAGCGGTATGACGCTCACTCAGGAGGTGAACGTGAGATCCAAAGAACTATGTTGGAACTTCTGAACCAGCTTGATGGATTTGATTCAAGAGGAGATGTTAAGGTCATCCTTGCAACAAACAGAATCGAGAGTCTTGACCCAGCACTGCTTCGACCTGGACGGATTGATAGGAAAATCGAGTTCCCTCTCCCAGATATCAAAACAAGAAGACGTATCTTCCAGATACACACATCGAAGATGACTCTCTCGGAAGATGTAAACCTCGAAGAGTTTGTGATGACGAAAGACGAGTTCTCAGGAGCTGATATAAAGGCTATATGCACTGAGGCTGGTCTACTAGCTCTCAGGGAGCGTCGTATGAAGGTGACACATCCTGATTTCAAGAAAGCAAAGGAGAAGGTTATGTTCAAGAAGAAAGAAGGCGTCCCTGAAGGCCTCTACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0002376 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007276 GO:0007292 GO:0008134 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009628 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009933 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010074 GO:0010078 GO:0010101 GO:0010102 GO:0010243 GO:0010311 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019827 GO:0019941 GO:0019953 GO:0022414 GO:0022607 GO:0022622 GO:0022624 GO:0030162 GO:0030163 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033554 GO:0034622 GO:0034976 GO:0035266 GO:0035966 GO:0036402 GO:0036503 GO:0040007 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043248 GO:0043254 GO:0043632 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044703 GO:0045087 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0046686 GO:0048232 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048532 GO:0048589 GO:0048609 GO:0048646 GO:0048731 GO:0048827 GO:0048829 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051704 GO:0051716 GO:0051788 GO:0060255 GO:0060260 GO:0060261 GO:0060968 GO:0061136 GO:0065003 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090351 GO:0090696 GO:0090697 GO:0090698 GO:0098727 GO:0099402 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05165 ko05169 ko05203 map03050 map05165 map05169 map05203
Pfam Domains
Protein Families
AAA

Protein Analysis

443

Amino Acids

49.37

Weight (kDa)

5.92

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 112 - 166 5.6e-11 Proteasomal ATPase OB C-terminal domain
AAA PF00004 225 - 358 1.7e-41 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 380 - 424 1.5e-11 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 881
AccB1I GGYRCC 2 cut(s) 91, 881
AccI GTMKAC 2 cut(s) 173, 1219
AciI CCGC 5 cut(s) 89, 143, 146, 722, 889
AclWI GGATC 4 cut(s) 218, 506, 519, 916
AcsI RAATTY 2 cut(s) 340, 730
AcyI GRCGYC 1 cut(s) 1308
AfaI GTAC 3 cut(s) 322, 401, 883
AfiI CCNNNNNNNGG 4 cut(s) 20, 56, 668, 698
AflIII ACRYGT 1 cut(s) 1326
AgsI TTSAA 7 cut(s) 28, 265, 443, 735, 972, 1267, 1294
AjnI CCWGG 3 cut(s) 445, 691, 1039
AjuI GAANNNNNNNTTGG 2 cut(s) 533, 565
AloI GAACNNNNNNTCC 2 cut(s) 906, 938
Alw26I GTCTC 1 cut(s) 145
AlwI GGATC 4 cut(s) 218, 506, 519, 916
AlwNI CAGNNNCTG 1 cut(s) 1187
AoxI GGCC 2 cut(s) 134, 1318
ApoI RAATTY 2 cut(s) 340, 730
Asp718I GGTACC 1 cut(s) 881
AspS9I GGNCC 4 cut(s) 11, 134, 392, 797
AsuHPI GGTGA 5 cut(s) 175, 698, 834, 923, 1261
AvaII GGWCC 3 cut(s) 11, 392, 797
AxyI CCTNAGG 1 cut(s) 270
BamHI GGATCC 1 cut(s) 511
BanI GGYRCC 2 cut(s) 91, 881
BauI CACGAG 1 cut(s) 102
BbsI GAAGAC 3 cut(s) 710, 847, 1099
BbvCI CCTCAGC 1 cut(s) 285
BccI CCATC 6 cut(s) 22, 47, 223, 788, 853, 953
BcgI CGANNNNNNTGC 4 cut(s) 23, 57, 445, 479
BciT130I CCWGG 3 cut(s) 447, 693, 1041
BcoDI GTCTC 1 cut(s) 145
BfaI CTAG 1 cut(s) 1223
BglII AGATCT 1 cut(s) 296
BisI GCNGC 3 cut(s) 90, 144, 147
BlsI GCNGC 3 cut(s) 91, 145, 148
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 3 cut(s) 447, 693, 1041
Bme18I GGWCC 3 cut(s) 11, 392, 797
BmgT120I GGNCC 4 cut(s) 11, 134, 392, 797
BmiI GGNNCC 3 cut(s) 93, 513, 883
BmrFI CCNGG 3 cut(s) 447, 693, 1041
BmrI ACTGGG 1 cut(s) 708
BmsI GCATC 2 cut(s) 453, 862
BmuI ACTGGG 1 cut(s) 708
BpiI GAAGAC 3 cut(s) 710, 847, 1099
Bpu10I CCTNAGC 3 cut(s) 285, 717, 801
BpuEI CTTGAG 1 cut(s) 772
BsaBI GATNNNNATC 2 cut(s) 363, 432
BsaHI GRCGYC 1 cut(s) 1308
BsaWI WCCGGW 1 cut(s) 189
BsaXI ACNNNNNCTCC 4 cut(s) 151, 181, 224, 254
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 56, 668, 698
Bse118I RCCGGY 2 cut(s) 85, 97
Bse1I ACTGG 1 cut(s) 703
Bse21I CCTNAGG 1 cut(s) 270
Bse3DI GCAATG 1 cut(s) 707
Bse8I GATNNNNATC 2 cut(s) 363, 432
BseBI CCWGG 3 cut(s) 447, 693, 1041
BseGI GGATG 4 cut(s) 455, 628, 990, 1255
BseJI GATNNNNATC 2 cut(s) 363, 432
BseLI CCNNNNNNNGG 4 cut(s) 20, 56, 668, 698
BseMI GCAATG 1 cut(s) 707
BseMII CTCAG 9 cut(s) 276, 284, 387, 599, 624, 918, 1194, 1200, 1243
BseNI ACTGG 1 cut(s) 703
BseRI GAGGAG 1 cut(s) 990
BseYI CCCAGC 1 cut(s) 1024
BshFI GGCC 2 cut(s) 136, 1320
BshNI GGYRCC 2 cut(s) 91, 881
BsiSI CCGG 3 cut(s) 86, 98, 190
BslFI GGGAC 2 cut(s) 18, 1295
BslI CCNNNNNNNGG 4 cut(s) 20, 56, 668, 698
BsmAI GTCTC 1 cut(s) 145
BsmFI GGGAC 2 cut(s) 18, 1295
BsnI GGCC 2 cut(s) 136, 1320
Bsp143I GATC 7 cut(s) 40, 210, 296, 310, 433, 511, 921
BspACI CCGC 5 cut(s) 89, 143, 146, 722, 889
BspANI GGCC 2 cut(s) 136, 1320
BspCNI CTCAG 9 cut(s) 277, 283, 388, 598, 625, 917, 1193, 1201, 1242
BspLI GGNNCC 3 cut(s) 93, 513, 883
BspPI GGATC 4 cut(s) 218, 506, 519, 916
BspT107I GGYRCC 2 cut(s) 91, 881
BsrDI GCAATG 1 cut(s) 707
BsrFI RCCGGY 2 cut(s) 85, 97
BsrI ACTGG 1 cut(s) 703
BssAI RCCGGY 2 cut(s) 85, 97
BssMI GATC 7 cut(s) 40, 210, 296, 310, 433, 511, 921
BssNI GRCGYC 1 cut(s) 1308
BssSI CACGAG 1 cut(s) 102
Bst2BI CACGAG 1 cut(s) 102
Bst2UI CCWGG 3 cut(s) 447, 693, 1041
Bst4CI ACNGT 1 cut(s) 163
Bst6I CTCTTC 4 cut(s) 231, 270, 342, 1146
BstACI GRCGYC 1 cut(s) 1308
BstC8I GCNNGC 2 cut(s) 87, 714
BstF5I GGATG 4 cut(s) 455, 628, 990, 1255
BstKTI GATC 7 cut(s) 43, 213, 299, 313, 436, 514, 924
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 7 cut(s) 40, 210, 296, 310, 433, 511, 921
BstMWI GCNNNNNNNGC 3 cut(s) 98, 124, 133
BstNI CCWGG 3 cut(s) 447, 693, 1041
BstNSI RCATGY 1 cut(s) 1330
BstSCI CCNGG 3 cut(s) 445, 691, 1039
BstV2I GAAGAC 3 cut(s) 710, 847, 1099
BstX2I RGATCY 3 cut(s) 296, 511, 921
BstXI CCANNNNNNTGG 1 cut(s) 959
BstYI RGATCY 3 cut(s) 296, 511, 921
Bsu36I CCTNAGG 1 cut(s) 270
BsuRI GGCC 2 cut(s) 136, 1320
BtsCI GGATG 4 cut(s) 455, 628, 990, 1255
BtsI GCAGTG 1 cut(s) 1028
BtsIMutI CAGTG 2 cut(s) 1028, 1206
Cac8I GCNNGC 2 cut(s) 87, 714
CaiI CAGNNNCTG 1 cut(s) 1187
Cfr10I RCCGGY 2 cut(s) 85, 97
Cfr13I GGNCC 4 cut(s) 11, 134, 392, 797
CseI GACGC 3 cut(s) 905, 1226, 1297
Csp6I GTAC 3 cut(s) 321, 400, 882
CviAII CATG 1 cut(s) 1327
CviQI GTAC 3 cut(s) 321, 400, 882
DpnI GATC 7 cut(s) 42, 212, 298, 312, 435, 513, 923
DpnII GATC 7 cut(s) 40, 210, 296, 310, 433, 511, 921
Eam1104I CTCTTC 4 cut(s) 231, 270, 342, 1146
EarI CTCTTC 4 cut(s) 231, 270, 342, 1146
Eco147I AGGCCT 1 cut(s) 1320
Eco32I GATATC 1 cut(s) 1081
Eco47I GGWCC 3 cut(s) 11, 392, 797
Eco81I CCTNAGG 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 730
EcoRII CCWGG 3 cut(s) 445, 691, 1039
EcoRV GATATC 1 cut(s) 1081
EcoT22I ATGCAT 1 cut(s) 468
FaeI CATG 1 cut(s) 1330
FalI AAGNNNNNCTT 2 cut(s) 423, 455
FaqI GGGAC 2 cut(s) 18, 1295
FatI CATG 1 cut(s) 1326
FauNDI CATATG 1 cut(s) 559
FblI GTMKAC 2 cut(s) 173, 1219
Fnu4HI GCNGC 3 cut(s) 90, 144, 147
FokI GGATG 4 cut(s) 462, 615, 977, 1242
Fsp4HI GCNGC 3 cut(s) 90, 144, 147
FspBI CTAG 1 cut(s) 1223
GluI GCNGC 3 cut(s) 90, 144, 147
GsaI CCCAGC 1 cut(s) 1028
HaeIII GGCC 2 cut(s) 136, 1320
HapII CCGG 3 cut(s) 86, 98, 190
HgaI GACGC 3 cut(s) 905, 1226, 1297
Hin1I GRCGYC 1 cut(s) 1308
Hin1II CATG 1 cut(s) 1330
HindIII AAGCTT 1 cut(s) 803
HpaII CCGG 3 cut(s) 86, 98, 190
HphI GGTGA 5 cut(s) 175, 698, 834, 923, 1261
Hpy166II GTNNAC 5 cut(s) 174, 323, 914, 1144, 1220
Hpy188I TCNGA 7 cut(s) 45, 159, 588, 780, 824, 948, 1135
Hpy8I GTNNAC 5 cut(s) 174, 323, 914, 1144, 1220
Hpy99I CGWCG 2 cut(s) 163, 1242
HpyAV CCTTC 5 cut(s) 430, 1240, 1276, 1298, 1310
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4IV ACGT 3 cut(s) 405, 916, 1096
HpyCH4V TGCA 3 cut(s) 466, 998, 1206
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 124, 133
HpySE526I ACGT 3 cut(s) 405, 916, 1096
Hsp92I GRCGYC 1 cut(s) 1308
Hsp92II CATG 1 cut(s) 1330
KpnI GGTACC 1 cut(s) 885
KroI GCCGGC 1 cut(s) 85
KroNI GCCGGC 1 cut(s) 87
Kzo9I GATC 7 cut(s) 40, 210, 296, 310, 433, 511, 921
LmnI GCTCC 4 cut(s) 192, 550, 1184, 1234
LweI GCATC 2 cut(s) 453, 862
MaeI CTAG 1 cut(s) 1223
MaeII ACGT 3 cut(s) 405, 916, 1096
MaeIII GTNAC 3 cut(s) 163, 192, 1249
MalI GATC 7 cut(s) 42, 212, 298, 312, 435, 513, 923
MboI GATC 7 cut(s) 40, 210, 296, 310, 433, 511, 921
MflI RGATCY 3 cut(s) 296, 511, 921
MluCI AATT 4 cut(s) 200, 340, 675, 730
MlyI GAGTC 5 cut(s) 160, 563, 619, 1024, 1120
MmeI TCCRAC 3 cut(s) 182, 370, 918
Mph1103I ATGCAT 1 cut(s) 468
MroNI GCCGGC 1 cut(s) 85
MseI TTAA 3 cut(s) 600, 657, 984
MspI CCGG 3 cut(s) 86, 98, 190
MspR9I CCNGG 3 cut(s) 447, 693, 1041
MvaI CCWGG 3 cut(s) 447, 693, 1041
MwoI GCNNNNNNNGC 3 cut(s) 98, 124, 133
NaeI GCCGGC 1 cut(s) 87
NdeI CATATG 1 cut(s) 559
NdeII GATC 7 cut(s) 40, 210, 296, 310, 433, 511, 921
NgoMIV GCCGGC 1 cut(s) 85
NlaIII CATG 1 cut(s) 1330
NlaIV GGNNCC 3 cut(s) 93, 513, 883
NmuCI GTSAC 2 cut(s) 163, 1249
NsiI ATGCAT 1 cut(s) 468
NspI RCATGY 1 cut(s) 1330
PceI AGGCCT 1 cut(s) 1320
PciI ACATGT 1 cut(s) 1326
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PdiI GCCGGC 1 cut(s) 87
PfeI GAWTC 8 cut(s) 28, 250, 364, 493, 589, 775, 968, 1008
PflFI GACNNNGTC 1 cut(s) 169
PkrI GCNGC 3 cut(s) 91, 145, 148
PleI GAGTC 5 cut(s) 160, 562, 619, 1023, 1120
PpsI GAGTC 5 cut(s) 160, 562, 619, 1023, 1120
PscI ACATGT 1 cut(s) 1326
Psp6I CCWGG 3 cut(s) 445, 691, 1039
PspFI CCCAGC 1 cut(s) 1024
PspGI CCWGG 3 cut(s) 445, 691, 1039
PspN4I GGNNCC 3 cut(s) 93, 513, 883
PspPI GGNCC 4 cut(s) 11, 134, 392, 797
PstNI CAGNNNCTG 1 cut(s) 1187
PsuI RGATCY 3 cut(s) 296, 511, 921
PsyI GACNNNGTC 1 cut(s) 169
RsaI GTAC 3 cut(s) 322, 401, 883
RsaNI GTAC 3 cut(s) 321, 400, 882
SaqAI TTAA 3 cut(s) 600, 657, 984
SatI GCNGC 3 cut(s) 90, 144, 147
Sau3AI GATC 7 cut(s) 40, 210, 296, 310, 433, 511, 921
Sau96I GGNCC 4 cut(s) 11, 134, 392, 797
ScaI AGTACT 1 cut(s) 401
SchI GAGTC 5 cut(s) 160, 563, 619, 1024, 1120
ScrFI CCNGG 3 cut(s) 447, 693, 1041
SfaNI GCATC 2 cut(s) 453, 862
SinI GGWCC 3 cut(s) 11, 392, 797
SmlI CTYRAG 1 cut(s) 751
SmoI CTYRAG 1 cut(s) 751
Sse9I AATT 4 cut(s) 200, 340, 675, 730
SseBI AGGCCT 1 cut(s) 1320
SsiI CCGC 5 cut(s) 89, 143, 146, 722, 889
SspMI CTAG 1 cut(s) 1223
StuI AGGCCT 1 cut(s) 1320
StyD4I CCNGG 3 cut(s) 445, 691, 1039
TaaI ACNGT 1 cut(s) 163
TaiI ACGT 3 cut(s) 408, 919, 1099
TaqI TCGA 6 cut(s) 455, 1011, 1036, 1062, 1118, 1149
TasI AATT 4 cut(s) 200, 340, 675, 730
TatI WGTACW 1 cut(s) 399
TauI GCSGC 3 cut(s) 92, 146, 149
TfiI GAWTC 8 cut(s) 28, 250, 364, 493, 589, 775, 968, 1008
Tru1I TTAA 3 cut(s) 600, 657, 984
Tru9I TTAA 3 cut(s) 600, 657, 984
TscAI CASTG 2 cut(s) 1035, 1213
TseFI GTSAC 2 cut(s) 163, 1249
Tsp45I GTSAC 2 cut(s) 163, 1249
TspDTI ATGAA 5 cut(s) 519, 545, 657, 847, 1259
TspGWI ACGGA 3 cut(s) 159, 223, 1060
TspRI CASTG 2 cut(s) 1035, 1213
Tth111I GACNNNGTC 1 cut(s) 169
VpaK11BI GGWCC 3 cut(s) 11, 392, 797
XapI RAATTY 2 cut(s) 340, 730
XceI RCATGY 1 cut(s) 1330
XmiI GTMKAC 2 cut(s) 173, 1219
XspI CTAG 1 cut(s) 1223
ZrmI AGTACT 1 cut(s) 401
Zsp2I ATGCAT 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.