FvH4_2g19750

26S proteasome regulatory subunit 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004988
Physical Location & Seq
Forward (+)
16682135 .. 16685689
3555 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19750.t1

Sequence Viewer

Length: 1341 bp
ATGGGTCAAGGAACTCCGGGCGGTTTGAACCGGCAGGGCCTACCGGGCGACCGAAAGCCCGACGGCAACGACAAGAAGGAGAAGAAGTACGAGCCGGCGGCTCCGCCGGCCCGCGTCGGACGCAAGCAGCGCAAACAGAAGGGTCCAGAAGCGGCGGCACGGCTTCCGACGGTGACGCCGCTGACCAAGTGCAAGCTCCGGCTACTGAAGCTGGAGCGGATTAAGGACTATCTGCTGATGGAGGAGGAGTTCGTGACGAACCAGGAGCGGCTGAAGCCTCAGGAGGAGAAGGCTGAGGAGGACAGATCTAAGGTCGACGATCTACGTGGCTCGCCGATGAGCGTCGGAAATCTGGAGGAGCTGATTGATGAGAACCACGCGATTGTTTCGTCGTCGGTGGGGCCAGAGTATTATGTGGGGATCCTCTCGTTTGTGGATAAGGACCAGTTGGAGCCTGGGTGTGCGATTTTGATGCACAATAAGGTGCTTTCTGTTGTTGGGCTTCTGCAAGATGAAGTTGATCCAATGGTGTCAGTAATGAAGGTCGAGAAAGCTCCCCTGGAGTCATATGCTGACATTGGTGGACTAGATGCCCAAATACAGGAGATTAAAGAAGCAGTGGAGCTCCCGCTGACACATCCCGAACTTTATGAAGACATTGGTATCAGGCCTCCCAAGGGAGTTATTTTGTATGGAGAGCCTGGAACTGGCAAGACCTTGCTTGCGAAGGCTGTGGCAAACTCAACATCAGCAACTTTCTTGCGTGTTGTTGGTAGTGAATTGATTCAAAAGTACTTGGGAGATGGTCCCAAGTTAGTGAGGGAGCTCTTTAGGGTTGCTGATGATCTCTCGCCTTCGATTGTGTTCATTGATGAAATCGATGCAGTTGGTACAAAGAGGTACGATGCCCATTCAGGTGGTGAACGAGAAATACAAAGGACTATGTTGGAGTTACTGAACCAATTGGACGGCTTTGATTCGAGAGGTGATGTCAAGGTGATTCTTGCAACAAACAGGATTGAAAGTCTTGACCCAGCCTTGCTTCGACCAGGACGAATTGATAGAAAGATTGAATTTCCACTTCCGGATATCAAAACAAGGAGGCGCATCTTTCAGATTCACACATCAAGGATGACATTGTCTGATGATGTTAACCTAGAGGAATTTGTCATGACTAAGGATGAGTTCTCTGGGGCTGATATAAAGGCTATATGCACCGAAGCTGGTCTTCTTGCATTGAGAGAGCGCCGAATGAAGGTGACACATCCAGACTTCAAGAAGGCCAAGGAGAAGGTTATGTTTAAGAAGAAGGAAGGTGTTCCAGAAGGACTATACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0002376 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007276 GO:0007292 GO:0008134 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009628 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009933 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010033 GO:0010035 GO:0010038 GO:0010073 GO:0010074 GO:0010078 GO:0010101 GO:0010102 GO:0010243 GO:0010311 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019827 GO:0019941 GO:0019953 GO:0022414 GO:0022607 GO:0022622 GO:0022624 GO:0030162 GO:0030163 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033554 GO:0034622 GO:0034976 GO:0035266 GO:0035966 GO:0036402 GO:0036503 GO:0040007 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043248 GO:0043254 GO:0043632 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044703 GO:0045087 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0046686 GO:0048232 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048532 GO:0048589 GO:0048609 GO:0048646 GO:0048731 GO:0048827 GO:0048829 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051704 GO:0051716 GO:0051788 GO:0060255 GO:0060260 GO:0060261 GO:0060968 GO:0061136 GO:0065003 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0090351 GO:0090696 GO:0090697 GO:0090698 GO:0098727 GO:0099402 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:1905392 GO:1905393 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05165 ko05169 ko05203 map03050 map05165 map05169 map05203
Pfam Domains
Protein Families
AAA

Protein Analysis

447

Amino Acids

49.78

Weight (kDa)

5.91

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 115 - 169 3.9e-10 Proteasomal ATPase OB C-terminal domain
AAA PF00004 228 - 361 1.7e-41 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 383 - 427 1.5e-11 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 217, 268
AccI GTMKAC 1 cut(s) 315
AccII CGCG 2 cut(s) 114, 380
AccIII TCCGGA 1 cut(s) 1084
AclWI GGATC 3 cut(s) 415, 428, 515
AcsI RAATTY 2 cut(s) 1073, 1163
AcuI CTGAAG 2 cut(s) 227, 293
AcyI GRCGYC 1 cut(s) 176
AfaI GTAC 4 cut(s) 89, 794, 892, 902
AfiI CCNNNNNNNGG 4 cut(s) 601, 677, 707, 1255
AflIII ACRYGT 1 cut(s) 1335
AgsI TTSAA 5 cut(s) 28, 788, 1022, 1073, 1276
AjnI CCWGG 5 cut(s) 261, 454, 558, 700, 1048
AloI GAACNNNNNNTCC 2 cut(s) 233, 265
AluBI AGCT 7 cut(s) 196, 211, 361, 554, 625, 826, 1223
AluI AGCT 7 cut(s) 196, 211, 361, 554, 625, 826, 1223
Alw21I GWGCWC 2 cut(s) 627, 828
AlwI GGATC 3 cut(s) 415, 428, 515
Aor13HI TCCGGA 1 cut(s) 1084
AoxI GGCC 5 cut(s) 37, 108, 401, 668, 1281
ApeKI GCWGC 1 cut(s) 127
ApoI RAATTY 2 cut(s) 1073, 1163
Asp700I GAANNNNTTC 2 cut(s) 783, 1317
AspLEI GCGC 3 cut(s) 132, 1107, 1248
AspS9I GGNCC 6 cut(s) 37, 109, 143, 401, 442, 806
AsuC2I CCSGG 2 cut(s) 18, 45
AsuHPI GGTGA 5 cut(s) 184, 932, 998, 1009, 1270
AvaII GGWCC 3 cut(s) 143, 442, 806
AxyI CCTNAGG 1 cut(s) 279
BaeI ACNNNNGTAYC 2 cut(s) 646, 679
BamHI GGATCC 1 cut(s) 420
BanII GRGCYC 2 cut(s) 627, 828
BbsI GAAGAC 2 cut(s) 660, 1220
Bbv12I GWGCWC 2 cut(s) 627, 828
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 139
BccI CCATC 2 cut(s) 232, 797
BceAI ACGGC 3 cut(s) 79, 176, 985
BcgI CGANNNNNNTGC 2 cut(s) 454, 488
BciT130I CCWGG 5 cut(s) 263, 456, 560, 702, 1050
BcnI CCSGG 2 cut(s) 18, 45
BfaI CTAG 2 cut(s) 587, 1157
BfoI RGCGCY 1 cut(s) 1249
BglI GCCNNNNNGGC 1 cut(s) 45
BglII AGATCT 1 cut(s) 305
BisI GCNGC 6 cut(s) 99, 128, 153, 156, 179, 269
BlsI GCNGC 6 cut(s) 100, 129, 154, 157, 180, 270
BmcAI AGTACT 1 cut(s) 794
Bme1390I CCNGG 7 cut(s) 18, 45, 263, 456, 560, 702, 1050
Bme18I GGWCC 3 cut(s) 143, 442, 806
BmgT120I GGNCC 6 cut(s) 37, 109, 143, 401, 442, 806
BmiI GGNNCC 6 cut(s) 102, 144, 402, 422, 453, 808
BmrFI CCNGG 7 cut(s) 18, 45, 263, 456, 560, 702, 1050
BmsI GCATC 5 cut(s) 462, 580, 871, 895, 1116
BpiI GAAGAC 2 cut(s) 660, 1220
BpmI CTGGAG 3 cut(s) 233, 374, 581
Bpu10I CCTNAGC 1 cut(s) 294
BpuMI CCSGG 2 cut(s) 18, 45
Bsa29I ATCGAT 1 cut(s) 879
BsaAI YACGTR 1 cut(s) 326
BsaHI GRCGYC 1 cut(s) 176
BsaJI CCNNGG 4 cut(s) 455, 558, 675, 1284
BsaWI WCCGGW 1 cut(s) 1084
BsaXI ACNNNNNCTCC 6 cut(s) 71, 101, 233, 263, 443, 473
Bsc4I CCNNNNNNNGG 4 cut(s) 601, 677, 707, 1255
Bse118I RCCGGY 3 cut(s) 30, 94, 106
Bse1I ACTGG 2 cut(s) 445, 712
Bse21I CCTNAGG 1 cut(s) 279
BseAI TCCGGA 1 cut(s) 1084
BseBI CCWGG 5 cut(s) 263, 456, 560, 702, 1050
BseCI ATCGAT 1 cut(s) 879
BseDI CCNNGG 4 cut(s) 455, 558, 675, 1284
BseGI GGATG 4 cut(s) 637, 1137, 1186, 1264
BseLI CCNNNNNNNGG 4 cut(s) 601, 677, 707, 1255
BseMII CTCAG 2 cut(s) 285, 293
BseNI ACTGG 2 cut(s) 445, 712
BseRI GAGGAG 5 cut(s) 257, 260, 299, 311, 371
BseXI GCAGC 1 cut(s) 139
BseYI CCCAGC 1 cut(s) 1033
Bsh1236I CGCG 2 cut(s) 114, 380
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 5 cut(s) 39, 110, 403, 670, 1283
BshVI ATCGAT 1 cut(s) 879
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 2 cut(s) 627, 828
BsiSI CCGG 7 cut(s) 17, 31, 44, 95, 107, 199, 1085
BslFI GGGAC 1 cut(s) 792
BslI CCNNNNNNNGG 4 cut(s) 601, 677, 707, 1255
BsmFI GGGAC 1 cut(s) 792
BsnI GGCC 5 cut(s) 39, 110, 403, 670, 1283
Bsp1286I GDGCHC 2 cut(s) 627, 828
Bsp13I TCCGGA 1 cut(s) 1084
Bsp143I GATC 5 cut(s) 305, 319, 420, 520, 844
BspANI GGCC 5 cut(s) 39, 110, 403, 670, 1283
BspCNI CTCAG 2 cut(s) 286, 292
BspDI ATCGAT 1 cut(s) 879
BspEI TCCGGA 1 cut(s) 1084
BspFNI CGCG 2 cut(s) 114, 380
BspHI TCATGA 1 cut(s) 1170
BspLI GGNNCC 6 cut(s) 102, 144, 402, 422, 453, 808
BspPI GGATC 3 cut(s) 415, 428, 515
BsrBI CCGCTC 2 cut(s) 217, 268
BsrFI RCCGGY 3 cut(s) 30, 94, 106
BsrI ACTGG 2 cut(s) 445, 712
BssAI RCCGGY 3 cut(s) 30, 94, 106
BssECI CCNNGG 4 cut(s) 455, 558, 675, 1284
BssMI GATC 5 cut(s) 305, 319, 420, 520, 844
BssNI GRCGYC 1 cut(s) 176
BssT1I CCWWGG 2 cut(s) 675, 1284
Bst2UI CCWGG 5 cut(s) 263, 456, 560, 702, 1050
Bst4CI ACNGT 1 cut(s) 172
BstACI GRCGYC 1 cut(s) 176
BstBAI YACGTR 1 cut(s) 326
BstC8I GCNNGC 7 cut(s) 96, 108, 112, 125, 194, 332, 723
BstDEI CTNAG 4 cut(s) 279, 294, 309, 1176
BstF5I GGATG 4 cut(s) 637, 1137, 1186, 1264
BstFNI CGCG 2 cut(s) 114, 380
BstH2I RGCGCY 1 cut(s) 1249
BstHHI GCGC 3 cut(s) 132, 1107, 1248
BstKTI GATC 5 cut(s) 308, 322, 423, 523, 847
BstMBI GATC 5 cut(s) 305, 319, 420, 520, 844
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 6 cut(s) 45, 107, 120, 129, 208, 274
BstNI CCWGG 5 cut(s) 263, 456, 560, 702, 1050
BstNSI RCATGY 1 cut(s) 1339
BstSCI CCNGG 7 cut(s) 16, 43, 261, 454, 558, 700, 1048
BstUI CGCG 2 cut(s) 114, 380
BstV1I GCAGC 1 cut(s) 139
BstV2I GAAGAC 2 cut(s) 660, 1220
BstX2I RGATCY 2 cut(s) 305, 420
BstXI CCANNNNNNTGG 1 cut(s) 917
BstYI RGATCY 2 cut(s) 305, 420
Bsu15I ATCGAT 1 cut(s) 879
Bsu36I CCTNAGG 1 cut(s) 279
BsuRI GGCC 5 cut(s) 39, 110, 403, 670, 1283
BsuTUI ATCGAT 1 cut(s) 879
BtsCI GGATG 4 cut(s) 637, 1137, 1186, 1264
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 1 cut(s) 624
Cac8I GCNNGC 7 cut(s) 96, 108, 112, 125, 194, 332, 723
CciI TCATGA 1 cut(s) 1170
CfoI GCGC 3 cut(s) 132, 1107, 1248
Cfr10I RCCGGY 3 cut(s) 30, 94, 106
Cfr13I GGNCC 6 cut(s) 37, 109, 143, 401, 442, 806
ClaI ATCGAT 1 cut(s) 879
CseI GACGC 4 cut(s) 103, 129, 184, 331
Csp6I GTAC 4 cut(s) 88, 793, 891, 901
CviAII CATG 2 cut(s) 1171, 1336
CviQI GTAC 4 cut(s) 88, 793, 891, 901
DdeI CTNAG 4 cut(s) 279, 294, 309, 1176
DpnI GATC 5 cut(s) 307, 321, 422, 522, 846
DpnII GATC 5 cut(s) 305, 319, 420, 520, 844
EciI GGCGGA 1 cut(s) 93
Ecl136II GAGCTC 2 cut(s) 625, 826
Eco130I CCWWGG 2 cut(s) 675, 1284
Eco147I AGGCCT 1 cut(s) 670
Eco24I GRGCYC 2 cut(s) 627, 828
Eco32I GATATC 1 cut(s) 1090
Eco47I GGWCC 3 cut(s) 143, 442, 806
Eco53kI GAGCTC 2 cut(s) 625, 826
Eco57I CTGAAG 2 cut(s) 227, 293
Eco81I CCTNAGG 1 cut(s) 279
EcoICRI GAGCTC 2 cut(s) 625, 826
EcoO109I RGGNCCY 1 cut(s) 37
EcoRII CCWGG 5 cut(s) 261, 454, 558, 700, 1048
EcoRV GATATC 1 cut(s) 1090
EcoT14I CCWWGG 2 cut(s) 675, 1284
EcoT38I GRGCYC 2 cut(s) 627, 828
ErhI CCWWGG 2 cut(s) 675, 1284
FaeI CATG 2 cut(s) 1174, 1339
FalI AAGNNNNNCTT 2 cut(s) 1212, 1244
FaqI GGGAC 1 cut(s) 792
FatI CATG 2 cut(s) 1170, 1335
FauI CCCGC 2 cut(s) 119, 636
FauNDI CATATG 1 cut(s) 568
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 6 cut(s) 99, 128, 153, 156, 179, 269
FokI GGATG 4 cut(s) 624, 1144, 1193, 1251
FriOI GRGCYC 2 cut(s) 627, 828
Fsp4HI GCNGC 6 cut(s) 99, 128, 153, 156, 179, 269
FspBI CTAG 2 cut(s) 587, 1157
GlaI GCGC 3 cut(s) 131, 1106, 1247
GluI GCNGC 6 cut(s) 99, 128, 153, 156, 179, 269
GsaI CCCAGC 1 cut(s) 1037
GsuI CTGGAG 3 cut(s) 233, 374, 581
HaeII RGCGCY 1 cut(s) 1249
HaeIII GGCC 5 cut(s) 39, 110, 403, 670, 1283
HapII CCGG 7 cut(s) 17, 31, 44, 95, 107, 199, 1085
HgaI GACGC 4 cut(s) 103, 129, 184, 331
HhaI GCGC 3 cut(s) 132, 1107, 1248
Hin1I GRCGYC 1 cut(s) 176
Hin1II CATG 2 cut(s) 1174, 1339
Hin6I GCGC 3 cut(s) 130, 1105, 1246
HinP1I GCGC 3 cut(s) 130, 1105, 1246
HincII GTYRAC 2 cut(s) 316, 1153
HindII GTYRAC 2 cut(s) 316, 1153
HinfI GANTC 5 cut(s) 563, 784, 977, 1000, 1117
HpaI GTTAAC 1 cut(s) 1153
HpaII CCGG 7 cut(s) 17, 31, 44, 95, 107, 199, 1085
HphI GGTGA 5 cut(s) 184, 932, 998, 1009, 1270
Hpy166II GTNNAC 4 cut(s) 316, 584, 923, 1153
Hpy188I TCNGA 5 cut(s) 119, 168, 347, 1116, 1144
Hpy8I GTNNAC 4 cut(s) 316, 584, 923, 1153
Hpy99I CGWCG 7 cut(s) 65, 119, 172, 320, 347, 394, 397
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 325
HpyCH4V TGCA 7 cut(s) 192, 475, 508, 884, 1007, 1215, 1235
HpyF10VI GCNNNNNNNGC 6 cut(s) 45, 107, 120, 129, 208, 274
HpyF3I CTNAG 4 cut(s) 279, 294, 309, 1176
HpySE526I ACGT 1 cut(s) 325
Hsp92I GRCGYC 1 cut(s) 176
Hsp92II CATG 2 cut(s) 1174, 1339
HspAI GCGC 3 cut(s) 130, 1105, 1246
Kpn2I TCCGGA 1 cut(s) 1084
KroI GCCGGC 2 cut(s) 94, 106
KroNI GCCGGC 2 cut(s) 96, 108
KspAI GTTAAC 1 cut(s) 1153
Kzo9I GATC 5 cut(s) 305, 319, 420, 520, 844
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 5 cut(s) 462, 580, 871, 895, 1116
MaeI CTAG 2 cut(s) 587, 1157
MaeII ACGT 1 cut(s) 325
MaeIII GTNAC 4 cut(s) 172, 253, 951, 1258
MalI GATC 5 cut(s) 307, 321, 422, 522, 846
MbiI CCGCTC 2 cut(s) 217, 268
MboI GATC 5 cut(s) 305, 319, 420, 520, 844
MboII GAAGA 4 cut(s) 94, 665, 1220, 1318
MfeI CAATTG 1 cut(s) 962
MflI RGATCY 2 cut(s) 305, 420
MhlI GDGCHC 2 cut(s) 627, 828
MluCI AATT 5 cut(s) 779, 962, 1056, 1073, 1163
MlyI GAGTC 1 cut(s) 572
MmeI TCCRAC 5 cut(s) 97, 191, 325, 429, 927
MroI TCCGGA 1 cut(s) 1084
MroNI GCCGGC 2 cut(s) 94, 106
MroXI GAANNNNTTC 2 cut(s) 783, 1317
MseI TTAA 4 cut(s) 222, 609, 1152, 1302
MslI CAYNNNNRTG 1 cut(s) 915
MspA1I CMGCKG 2 cut(s) 181, 631
MspI CCGG 7 cut(s) 17, 31, 44, 95, 107, 199, 1085
MspR9I CCNGG 7 cut(s) 18, 45, 263, 456, 560, 702, 1050
MunI CAATTG 1 cut(s) 962
MvaI CCWGG 5 cut(s) 263, 456, 560, 702, 1050
MvnI CGCG 2 cut(s) 114, 380
MwoI GCNNNNNNNGC 6 cut(s) 45, 107, 120, 129, 208, 274
NaeI GCCGGC 2 cut(s) 96, 108
NciI CCSGG 2 cut(s) 18, 45
NdeI CATATG 1 cut(s) 568
NdeII GATC 5 cut(s) 305, 319, 420, 520, 844
NgoMIV GCCGGC 2 cut(s) 94, 106
NlaIII CATG 2 cut(s) 1174, 1339
NlaIV GGNNCC 6 cut(s) 102, 144, 402, 422, 453, 808
NmuCI GTSAC 3 cut(s) 172, 253, 1258
NspI RCATGY 1 cut(s) 1339
PagI TCATGA 1 cut(s) 1170
PceI AGGCCT 1 cut(s) 670
PciI ACATGT 1 cut(s) 1335
PcsI WCGNNNNNNNCGW 1 cut(s) 1051
PdiI GCCGGC 2 cut(s) 96, 108
PdmI GAANNNNTTC 2 cut(s) 783, 1317
PfeI GAWTC 4 cut(s) 784, 977, 1000, 1117
PflFI GACNNNGTC 1 cut(s) 1138
PkrI GCNGC 6 cut(s) 100, 129, 154, 157, 180, 270
PleI GAGTC 1 cut(s) 571
PpsI GAGTC 1 cut(s) 571
Ppu21I YACGTR 1 cut(s) 326
PscI ACATGT 1 cut(s) 1335
Psp124BI GAGCTC 2 cut(s) 627, 828
Psp6I CCWGG 5 cut(s) 261, 454, 558, 700, 1048
PspFI CCCAGC 1 cut(s) 1033
PspGI CCWGG 5 cut(s) 261, 454, 558, 700, 1048
PspN4I GGNNCC 6 cut(s) 102, 144, 402, 422, 453, 808
PspPI GGNCC 6 cut(s) 37, 109, 143, 401, 442, 806
PsrI GAACNNNNNNTAC 2 cut(s) 915, 947
PsuI RGATCY 2 cut(s) 305, 420
PsyI GACNNNGTC 1 cut(s) 1138
RsaI GTAC 4 cut(s) 89, 794, 892, 902
RsaNI GTAC 4 cut(s) 88, 793, 891, 901
RseI CAYNNNNRTG 1 cut(s) 915
SacI GAGCTC 2 cut(s) 627, 828
SalI GTCGAC 1 cut(s) 314
SaqAI TTAA 4 cut(s) 222, 609, 1152, 1302
SatI GCNGC 6 cut(s) 99, 128, 153, 156, 179, 269
Sau3AI GATC 5 cut(s) 305, 319, 420, 520, 844
Sau96I GGNCC 6 cut(s) 37, 109, 143, 401, 442, 806
ScaI AGTACT 1 cut(s) 794
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 7 cut(s) 18, 45, 263, 456, 560, 702, 1050
SduI GDGCHC 2 cut(s) 627, 828
SfaNI GCATC 5 cut(s) 462, 580, 871, 895, 1116
SinI GGWCC 3 cut(s) 143, 442, 806
SmiMI CAYNNNNRTG 1 cut(s) 915
Sse9I AATT 5 cut(s) 779, 962, 1056, 1073, 1163
SseBI AGGCCT 1 cut(s) 670
SspMI CTAG 2 cut(s) 587, 1157
SstI GAGCTC 2 cut(s) 627, 828
StuI AGGCCT 1 cut(s) 670
StyD4I CCNGG 7 cut(s) 16, 43, 261, 454, 558, 700, 1048
StyI CCWWGG 2 cut(s) 675, 1284
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 1 cut(s) 328
TaqI TCGA 6 cut(s) 315, 546, 857, 879, 980, 1045
TaqII GACCGA 1 cut(s) 66
TasI AATT 5 cut(s) 779, 962, 1056, 1073, 1163
TatI WGTACW 1 cut(s) 792
TauI GCSGC 5 cut(s) 101, 155, 158, 181, 271
TfiI GAWTC 4 cut(s) 784, 977, 1000, 1117
Tru1I TTAA 4 cut(s) 222, 609, 1152, 1302
Tru9I TTAA 4 cut(s) 222, 609, 1152, 1302
TscAI CASTG 1 cut(s) 624
TseFI GTSAC 3 cut(s) 172, 253, 1258
TseI GCWGC 1 cut(s) 127
Tsp45I GTSAC 3 cut(s) 172, 253, 1258
TspDTI ATGAA 6 cut(s) 528, 554, 666, 856, 888, 1268
TspRI CASTG 1 cut(s) 624
Tth111I GACNNNGTC 1 cut(s) 1138
VpaK11BI GGWCC 3 cut(s) 143, 442, 806
XapI RAATTY 2 cut(s) 1073, 1163
XceI RCATGY 1 cut(s) 1339
XcmI CCANNNNNNNNNTGG 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 2 cut(s) 783, 1317
XspI CTAG 2 cut(s) 587, 1157
ZrmI AGTACT 1 cut(s) 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.