AT4G30320

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0017048
Physical Location & Seq
Forward (+)
14835792 .. 14836531
740 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30320.1

Sequence Viewer

Length: 486 bp
ATGGCTTTTCCTAGTTGTGTTTCGGTTGCAATCACGGCTATGATGCTACTTGTAACATGTTGTCATTGTGCAACATACCAAGAACAATTCATGGGTCCACAAAACGCAGCAAGAGCTCATTTAAGGCTCAAACCGCTAAAGTGGGACGCCAAATTGGCCCGTTACGCGCAATGGTGGGCTAACCAGAGACGCGGTGACTGCGCCTTGACCCATTCCAATGGACCCTATGGTGAGAATCTCTTTTGGGGCTCAGGCAATAGATGGGGTCCATCTCAAGCCGCCTATGGATGGTTGTCTGAAGCAAGGAGCTATAACTATAGATCTAACTCGTGCAACTCCGAGATGTGTGGCCATTACACACAAATCGTGTGGAAGAACACACAAAAGATTGGTTGCGCTCACGTGATCTGCAATGGCGGAGGAGGCGTATTCTTGACTTGCAATTATGATCCACCGGGGAATTTTCTTGGTAGGAAGCCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP

Protein Analysis

161

Amino Acids

18.03

Weight (kDa)

9.07

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 34 - 149 4.7e-21 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30320
fragaria_vesca FvH4_4g09950
malus_domestica MD16G1266300.v1.1
prunus_persica Prupe.1G091300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0403501
rosa_laevigata RLG00000008972
rosa_multiflora Rmu_sc0007727.1_g000034
rosa_rugosa Rorug04G0043800
rosa_samantha Rh4AG119200 Rh4BG112300 Rh4CG127000 Rh4DG111900
rosa_wichuraiana Rw4G009670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 167, 192
AciI CCGC 4 cut(s) 134, 192, 279, 417
AclWI GGATC 1 cut(s) 443
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 1 cut(s) 460
AcuI CTGAAG 1 cut(s) 318
AcvI CACGTG 1 cut(s) 403
AcyI GRCGYC 1 cut(s) 147
AfiI CCNNNNNNNGG 1 cut(s) 288
AflIII ACRYGT 1 cut(s) 56
AluBI AGCT 2 cut(s) 116, 309
AluI AGCT 2 cut(s) 116, 309
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 1 cut(s) 181
AlwI GGATC 1 cut(s) 443
AoxI GGCC 2 cut(s) 156, 349
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 1 cut(s) 460
AspLEI GCGC 3 cut(s) 169, 203, 398
AspS9I GGNCC 4 cut(s) 95, 157, 221, 266
AsuC2I CCSGG 1 cut(s) 456
AsuHPI GGTGA 2 cut(s) 206, 242
AvaII GGWCC 3 cut(s) 95, 221, 266
BalI TGGCCA 1 cut(s) 351
BanII GRGCYC 2 cut(s) 118, 251
BauI CACGAG 1 cut(s) 328
BbrPI CACGTG 1 cut(s) 403
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 1 cut(s) 119
BccI CCATC 3 cut(s) 255, 277, 282
BceAI ACGGC 1 cut(s) 51
BcnI CCSGG 1 cut(s) 456
BcoDI GTCTC 1 cut(s) 181
BfaI CTAG 1 cut(s) 12
BfmI CTRYAG 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 155
BglII AGATCT 1 cut(s) 320
BisI GCNGC 2 cut(s) 108, 279
BlsI GCNGC 2 cut(s) 109, 280
Bme1390I CCNGG 1 cut(s) 456
Bme18I GGWCC 3 cut(s) 95, 221, 266
BmgT120I GGNCC 4 cut(s) 95, 157, 221, 266
BmiI GGNNCC 3 cut(s) 96, 223, 267
BmrFI CCNGG 1 cut(s) 456
BmsI GCATC 1 cut(s) 33
Bpu10I CCTNAGC 1 cut(s) 250
BpuEI CTTGAG 1 cut(s) 258
BpuMI CCSGG 1 cut(s) 456
BsaAI YACGTR 1 cut(s) 403
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 455
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bsc4I CCNNNNNNNGG 1 cut(s) 288
Bse3DI GCAATG 2 cut(s) 176, 418
BseDI CCNNGG 1 cut(s) 455
BseGI GGATG 1 cut(s) 293
BseLI CCNNNNNNNGG 1 cut(s) 288
BseMI GCAATG 2 cut(s) 176, 418
BseMII CTCAG 1 cut(s) 264
BseRI GAGGAG 1 cut(s) 435
BseXI GCAGC 1 cut(s) 119
Bsh1236I CGCG 2 cut(s) 167, 192
BshFI GGCC 2 cut(s) 158, 351
BsiHKAI GWGCWC 1 cut(s) 118
BsiSI CCGG 1 cut(s) 455
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 1 cut(s) 288
BsmAI GTCTC 1 cut(s) 181
BsmBI CGTCTC 1 cut(s) 181
BsmFI GGGAC 1 cut(s) 158
BsnI GGCC 2 cut(s) 158, 351
Bsp1286I GDGCHC 2 cut(s) 118, 251
Bsp143I GATC 3 cut(s) 320, 405, 448
BspACI CCGC 4 cut(s) 134, 192, 279, 417
BspANI GGCC 2 cut(s) 158, 351
BspCNI CTCAG 1 cut(s) 263
BspFNI CGCG 2 cut(s) 167, 192
BspLI GGNNCC 3 cut(s) 96, 223, 267
BspPI GGATC 1 cut(s) 443
BsrDI GCAATG 2 cut(s) 176, 418
BssECI CCNNGG 1 cut(s) 455
BssMI GATC 3 cut(s) 320, 405, 448
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 328
Bst2BI CACGAG 1 cut(s) 328
BstACI GRCGYC 1 cut(s) 147
BstBAI YACGTR 1 cut(s) 403
BstDEI CTNAG 1 cut(s) 250
BstF5I GGATG 1 cut(s) 293
BstFNI CGCG 2 cut(s) 167, 192
BstHHI GCGC 3 cut(s) 169, 203, 398
BstKTI GATC 3 cut(s) 323, 408, 451
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 3 cut(s) 320, 405, 448
BstMWI GCNNNNNNNGC 7 cut(s) 35, 113, 133, 155, 164, 198, 423
BstNSI RCATGY 1 cut(s) 60
BstSCI CCNGG 1 cut(s) 454
BstSFI CTRYAG 1 cut(s) 316
BstUI CGCG 2 cut(s) 167, 192
BstV1I GCAGC 1 cut(s) 119
BstX2I RGATCY 1 cut(s) 320
BstXI CCANNNNNNTGG 1 cut(s) 218
BstYI RGATCY 1 cut(s) 320
BsuRI GGCC 2 cut(s) 158, 351
BtsCI GGATG 1 cut(s) 293
CfoI GCGC 3 cut(s) 169, 203, 398
Cfr13I GGNCC 4 cut(s) 95, 157, 221, 266
CseI GACGC 2 cut(s) 155, 198
CspCI CAANNNNNGTGG 2 cut(s) 350, 385
CviAII CATG 2 cut(s) 57, 91
DdeI CTNAG 1 cut(s) 250
DpnI GATC 3 cut(s) 322, 407, 450
DpnII GATC 3 cut(s) 320, 405, 448
EaeI YGGCCR 1 cut(s) 349
EciI GGCGGA 1 cut(s) 432
Ecl136II GAGCTC 1 cut(s) 116
Eco24I GRGCYC 2 cut(s) 118, 251
Eco47I GGWCC 3 cut(s) 95, 221, 266
Eco53kI GAGCTC 1 cut(s) 116
Eco57I CTGAAG 1 cut(s) 318
Eco72I CACGTG 1 cut(s) 403
EcoICRI GAGCTC 1 cut(s) 116
EcoT38I GRGCYC 2 cut(s) 118, 251
Esp3I CGTCTC 1 cut(s) 181
FaeI CATG 2 cut(s) 60, 94
FaiI YATR 9 cut(s) 41, 58, 76, 92, 228, 285, 312, 318, 447
FaqI GGGAC 1 cut(s) 158
FatI CATG 2 cut(s) 56, 90
Fnu4HI GCNGC 2 cut(s) 108, 279
FokI GGATG 1 cut(s) 300
FriOI GRGCYC 2 cut(s) 118, 251
Fsp4HI GCNGC 2 cut(s) 108, 279
FspBI CTAG 1 cut(s) 12
GlaI GCGC 3 cut(s) 168, 202, 397
GluI GCNGC 2 cut(s) 108, 279
HaeIII GGCC 2 cut(s) 158, 351
HapII CCGG 1 cut(s) 455
HgaI GACGC 2 cut(s) 155, 198
HhaI GCGC 3 cut(s) 169, 203, 398
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 2 cut(s) 60, 94
Hin6I GCGC 3 cut(s) 167, 201, 396
HinP1I GCGC 3 cut(s) 167, 201, 396
HinfI GANTC 1 cut(s) 235
HpaII CCGG 1 cut(s) 455
HphI GGTGA 2 cut(s) 206, 242
Hpy166II GTNNAC 1 cut(s) 98
Hpy188I TCNGA 2 cut(s) 298, 340
Hpy188III TCNNGA 1 cut(s) 433
Hpy8I GTNNAC 1 cut(s) 98
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 5 cut(s) 29, 71, 333, 411, 441
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 113, 133, 155, 164, 198, 423
HpyF3I CTNAG 1 cut(s) 250
HpySE526I ACGT 1 cut(s) 402
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 2 cut(s) 60, 94
HspAI GCGC 3 cut(s) 167, 201, 396
Kzo9I GATC 3 cut(s) 320, 405, 448
LmnI GCTCC 1 cut(s) 306
LpnPI CCDG 3 cut(s) 197, 237, 468
Lsp1109I GCAGC 1 cut(s) 119
LweI GCATC 1 cut(s) 33
MaeI CTAG 1 cut(s) 12
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 3 cut(s) 52, 161, 194
MalI GATC 3 cut(s) 322, 407, 450
MboI GATC 3 cut(s) 320, 405, 448
MboII GAAGA 1 cut(s) 385
MflI RGATCY 1 cut(s) 320
MhlI GDGCHC 2 cut(s) 118, 251
MlsI TGGCCA 1 cut(s) 351
MluCI AATT 4 cut(s) 86, 152, 442, 460
MluNI TGGCCA 1 cut(s) 351
MnlI CCTC 2 cut(s) 413, 416
Mox20I TGGCCA 1 cut(s) 351
MscI TGGCCA 1 cut(s) 351
MseI TTAA 1 cut(s) 122
MslI CAYNNNNRTG 2 cut(s) 38, 216
Msp20I TGGCCA 1 cut(s) 351
MspI CCGG 1 cut(s) 455
MspR9I CCNGG 1 cut(s) 456
MvnI CGCG 2 cut(s) 167, 192
MwoI GCNNNNNNNGC 7 cut(s) 35, 113, 133, 155, 164, 198, 423
NciI CCSGG 1 cut(s) 456
NdeII GATC 3 cut(s) 320, 405, 448
NlaIII CATG 2 cut(s) 60, 94
NlaIV GGNNCC 3 cut(s) 96, 223, 267
NmuCI GTSAC 1 cut(s) 194
NspI RCATGY 1 cut(s) 60
PciI ACATGT 1 cut(s) 56
PfeI GAWTC 1 cut(s) 235
PkrI GCNGC 2 cut(s) 109, 280
PmaCI CACGTG 1 cut(s) 403
PmlI CACGTG 1 cut(s) 403
Ppu21I YACGTR 1 cut(s) 403
PscI ACATGT 1 cut(s) 56
Psp124BI GAGCTC 1 cut(s) 118
PspCI CACGTG 1 cut(s) 403
PspN4I GGNNCC 3 cut(s) 96, 223, 267
PspPI GGNCC 4 cut(s) 95, 157, 221, 266
PsuI RGATCY 1 cut(s) 320
RseI CAYNNNNRTG 2 cut(s) 38, 216
SacI GAGCTC 1 cut(s) 118
SaqAI TTAA 1 cut(s) 122
SatI GCNGC 2 cut(s) 108, 279
Sau3AI GATC 3 cut(s) 320, 405, 448
Sau96I GGNCC 4 cut(s) 95, 157, 221, 266
ScrFI CCNGG 1 cut(s) 456
SduI GDGCHC 2 cut(s) 118, 251
SetI ASST 3 cut(s) 118, 311, 405
SfaNI GCATC 1 cut(s) 33
SfcI CTRYAG 1 cut(s) 316
SinI GGWCC 3 cut(s) 95, 221, 266
SmiMI CAYNNNNRTG 2 cut(s) 38, 216
SmlI CTYRAG 1 cut(s) 273
SmoI CTYRAG 1 cut(s) 273
Sse9I AATT 4 cut(s) 86, 152, 442, 460
SsiI CCGC 4 cut(s) 134, 192, 279, 417
SspMI CTAG 1 cut(s) 12
SstI GAGCTC 1 cut(s) 118
StyD4I CCNGG 1 cut(s) 454
TaiI ACGT 1 cut(s) 405
TasI AATT 4 cut(s) 86, 152, 442, 460
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 1 cut(s) 235
Tru1I TTAA 1 cut(s) 122
Tru9I TTAA 1 cut(s) 122
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 1 cut(s) 107
Tsp45I GTSAC 1 cut(s) 194
TspDTI ATGAA 1 cut(s) 79
VpaK11BI GGWCC 3 cut(s) 95, 221, 266
XapI RAATTY 1 cut(s) 460
XceI RCATGY 1 cut(s) 60
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.