MD16G1266300.v1.1

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0017048
Physical Location & Seq
Forward (+)
33930996 .. 33931511
516 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1266300.v1.1.491

Sequence Viewer

Length: 516 bp
ATGCATATAACATTGTACTACTGCTTCACCATCACCATTTTTTTGCTCCTTAATATCTCAAGAATTGCAGATGCTGCAACAAGGCAAACCAAGTACCTAAGCCAGGCCAACCAATTTATGGATCCTCAAAATGCTGCTCGGTCAGCCAACAGAATCCCACCATTGGCGTGGGATGCAAAGTTGGCAAAGTATGCACAATGGTACGCAAGCCAAAGGCGGTTCGACTGCGCGTTGAGGCACTCGAACGGGCCTTACGGGGAGAACATTTTCTGGGGGAGTGGCATCGAGTGGACGCCAGCTCAGGCAGTGACGGCTTGGGTTTCAGAACGGAAGTCTTACAATTACTGGGCAAATTCTTGTGCTACAAGGCAGCAATGTGGGCATTATACACAGATTGTGTGGAGGTCAACAAGCAAAGTTGGCTGTGCTAGAGTCAATTGTTTTGGTGGTAGGGGTGTCTTCATGGTTTGCAACTATGACCCTCCGGGGAATTACATTGGAGAAAGGCCTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAP

Protein Analysis

172

Amino Acids

19.59

Weight (kDa)

9.35

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 41 - 159 1.2e-21 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30320
fragaria_vesca FvH4_4g09950
malus_domestica MD16G1266300.v1.1
prunus_persica Prupe.1G091300_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0403501
rosa_laevigata RLG00000008972
rosa_multiflora Rmu_sc0007727.1_g000034
rosa_rugosa Rorug04G0043800
rosa_samantha Rh4AG119200 Rh4BG112300 Rh4CG127000 Rh4DG111900
rosa_wichuraiana Rw4G009670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 118
AccII CGCG 1 cut(s) 230
AciI CCGC 1 cut(s) 217
AclWI GGATC 2 cut(s) 116, 129
AcsI RAATTY 1 cut(s) 352
AcyI GRCGYC 1 cut(s) 293
AfaI GTAC 3 cut(s) 17, 95, 203
AfiI CCNNNNNNNGG 3 cut(s) 103, 118, 163
AjnI CCWGG 1 cut(s) 102
AluBI AGCT 1 cut(s) 299
AluI AGCT 1 cut(s) 299
AlwI GGATC 2 cut(s) 116, 129
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 3 cut(s) 105, 248, 506
ApeKI GCWGC 3 cut(s) 74, 134, 370
ApoI RAATTY 1 cut(s) 352
Asp700I GAANNNNTTC 1 cut(s) 266
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 248
AsuC2I CCSGG 1 cut(s) 486
AsuHPI GGTGA 2 cut(s) 19, 25
BamHI GGATCC 1 cut(s) 121
BarI GAAGNNNNNNTAC 2 cut(s) 8, 40
BbsI GAAGAC 1 cut(s) 451
BbvI GCAGC 3 cut(s) 61, 121, 382
BccI CCATC 1 cut(s) 38
BceAI ACGGC 1 cut(s) 327
BciT130I CCWGG 1 cut(s) 104
BcnI CCSGG 1 cut(s) 486
BfaI CTAG 1 cut(s) 429
BisI GCNGC 3 cut(s) 75, 135, 371
BlsI GCNGC 3 cut(s) 76, 136, 372
Bme1390I CCNGG 2 cut(s) 104, 486
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 1 cut(s) 123
BmrFI CCNGG 2 cut(s) 104, 486
BmrI ACTGGG 1 cut(s) 355
BmsI GCATC 3 cut(s) 61, 163, 291
BmuI ACTGGG 1 cut(s) 355
BpiI GAAGAC 1 cut(s) 451
Bpu10I CCTNAGC 2 cut(s) 98, 300
BpuEI CTTGAG 1 cut(s) 43
BpuMI CCSGG 1 cut(s) 486
BsaHI GRCGYC 1 cut(s) 293
BsaJI CCNNGG 1 cut(s) 485
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 118, 163
Bse1I ACTGG 1 cut(s) 350
Bse3DI GCAATG 1 cut(s) 380
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 1 cut(s) 485
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 3 cut(s) 103, 118, 163
BseMI GCAATG 1 cut(s) 380
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 1 cut(s) 350
BseXI GCAGC 3 cut(s) 61, 121, 382
Bsh1236I CGCG 1 cut(s) 230
BshFI GGCC 3 cut(s) 107, 250, 508
BsiSI CCGG 1 cut(s) 485
BslI CCNNNNNNNGG 3 cut(s) 103, 118, 163
BsnI GGCC 3 cut(s) 107, 250, 508
Bsp143I GATC 1 cut(s) 121
BspACI CCGC 1 cut(s) 217
BspANI GGCC 3 cut(s) 107, 250, 508
BspCNI CTCAG 1 cut(s) 313
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 1 cut(s) 123
BspPI GGATC 2 cut(s) 116, 129
BsrDI GCAATG 1 cut(s) 380
BsrI ACTGG 1 cut(s) 350
BssECI CCNNGG 1 cut(s) 485
BssMI GATC 1 cut(s) 121
BssNI GRCGYC 1 cut(s) 293
Bst2UI CCWGG 1 cut(s) 104
BstACI GRCGYC 1 cut(s) 293
BstAPI GCANNNNNTGC 2 cut(s) 74, 191
BstC8I GCNNGC 2 cut(s) 208, 297
BstDEI CTNAG 2 cut(s) 98, 300
BstENI CCTNNNNNAGG 1 cut(s) 101
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 1 cut(s) 230
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 1 cut(s) 124
BstMBI GATC 1 cut(s) 121
BstMWI GCNNNNNNNGC 8 cut(s) 74, 143, 173, 182, 191, 311, 379, 420
BstNI CCWGG 1 cut(s) 104
BstSCI CCNGG 2 cut(s) 102, 484
BstUI CGCG 1 cut(s) 230
BstV1I GCAGC 3 cut(s) 61, 121, 382
BstV2I GAAGAC 1 cut(s) 451
BstX2I RGATCY 1 cut(s) 121
BstXI CCANNNNNNTGG 1 cut(s) 168
BstYI RGATCY 1 cut(s) 121
BsuRI GGCC 3 cut(s) 107, 250, 508
BtsCI GGATG 1 cut(s) 178
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 1 cut(s) 312
Cac8I GCNNGC 2 cut(s) 208, 297
CaiI CAGNNNCTG 1 cut(s) 74
CfoI GCGC 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 248
CseI GACGC 1 cut(s) 301
Csp6I GTAC 3 cut(s) 16, 94, 202
CviAII CATG 1 cut(s) 463
CviJI RGCY 9 cut(s) 102, 107, 146, 210, 250, 299, 314, 423, 508
CviKI_1 RGCY 9 cut(s) 102, 107, 146, 210, 250, 299, 314, 423, 508
CviQI GTAC 3 cut(s) 16, 94, 202
DdeI CTNAG 2 cut(s) 98, 300
DpnI GATC 1 cut(s) 123
DpnII GATC 1 cut(s) 121
Eco147I AGGCCT 1 cut(s) 508
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 102
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 1 cut(s) 466
FaiI YATR 7 cut(s) 6, 8, 119, 192, 387, 464, 477
FatI CATG 1 cut(s) 462
Fnu4HI GCNGC 3 cut(s) 75, 135, 371
FokI GGATG 1 cut(s) 185
Fsp4HI GCNGC 3 cut(s) 75, 135, 371
FspBI CTAG 1 cut(s) 429
GlaI GCGC 1 cut(s) 229
GluI GCNGC 3 cut(s) 75, 135, 371
HaeIII GGCC 3 cut(s) 107, 250, 508
HapII CCGG 1 cut(s) 485
HgaI GACGC 1 cut(s) 301
HhaI GCGC 1 cut(s) 230
Hin1I GRCGYC 1 cut(s) 293
Hin1II CATG 1 cut(s) 466
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 2 cut(s) 153, 432
HpaII CCGG 1 cut(s) 485
HphI GGTGA 2 cut(s) 19, 25
Hpy166II GTNNAC 2 cut(s) 291, 408
Hpy188I TCNGA 1 cut(s) 325
Hpy188III TCNNGA 1 cut(s) 60
Hpy8I GTNNAC 2 cut(s) 291, 408
HpyCH4V TGCA 6 cut(s) 4, 68, 77, 176, 194, 471
HpyF10VI GCNNNNNNNGC 8 cut(s) 74, 143, 173, 182, 191, 311, 379, 420
HpyF3I CTNAG 2 cut(s) 98, 300
Hsp92I GRCGYC 1 cut(s) 293
Hsp92II CATG 1 cut(s) 466
HspAI GCGC 1 cut(s) 228
Kzo9I GATC 1 cut(s) 121
LmnI GCTCC 1 cut(s) 51
LpnPI CCDG 7 cut(s) 89, 116, 256, 287, 309, 331, 498
Lsp1109I GCAGC 3 cut(s) 61, 121, 382
LweI GCATC 3 cut(s) 61, 163, 291
MaeI CTAG 1 cut(s) 429
MaeIII GTNAC 1 cut(s) 307
MalI GATC 1 cut(s) 123
MboI GATC 1 cut(s) 121
MboII GAAGA 1 cut(s) 451
MfeI CAATTG 1 cut(s) 436
MflI RGATCY 1 cut(s) 121
MluCI AATT 6 cut(s) 63, 113, 340, 352, 436, 490
MlyI GAGTC 1 cut(s) 441
MnlI CCTC 4 cut(s) 135, 228, 396, 492
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 266
MseI TTAA 1 cut(s) 51
MslI CAYNNNNRTG 1 cut(s) 166
MspI CCGG 1 cut(s) 485
MspR9I CCNGG 2 cut(s) 104, 486
MunI CAATTG 1 cut(s) 436
MvaI CCWGG 1 cut(s) 104
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 8 cut(s) 74, 143, 173, 182, 191, 311, 379, 420
NciI CCSGG 1 cut(s) 486
NdeII GATC 1 cut(s) 121
NlaIII CATG 1 cut(s) 466
NlaIV GGNNCC 1 cut(s) 123
NmuCI GTSAC 1 cut(s) 307
NsiI ATGCAT 1 cut(s) 6
PceI AGGCCT 1 cut(s) 508
PdmI GAANNNNTTC 1 cut(s) 266
PfeI GAWTC 1 cut(s) 153
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 3 cut(s) 76, 136, 372
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 1 cut(s) 248
PsrI GAACNNNNNNTAC 2 cut(s) 236, 268
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 3 cut(s) 17, 95, 203
RsaNI GTAC 3 cut(s) 16, 94, 202
RseI CAYNNNNRTG 1 cut(s) 166
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 3 cut(s) 75, 135, 371
Sau3AI GATC 1 cut(s) 121
Sau96I GGNCC 1 cut(s) 248
SchI GAGTC 1 cut(s) 441
ScrFI CCNGG 2 cut(s) 104, 486
SetI ASST 3 cut(s) 99, 301, 407
SfaNI GCATC 3 cut(s) 61, 163, 291
SmiMI CAYNNNNRTG 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
Sse9I AATT 6 cut(s) 63, 113, 340, 352, 436, 490
SseBI AGGCCT 1 cut(s) 508
SsiI CCGC 1 cut(s) 217
SspMI CTAG 1 cut(s) 429
StuI AGGCCT 1 cut(s) 508
StyD4I CCNGG 2 cut(s) 102, 484
TaqI TCGA 3 cut(s) 222, 242, 285
TaqII GACCGA 1 cut(s) 129
TasI AATT 6 cut(s) 63, 113, 340, 352, 436, 490
TatI WGTACW 1 cut(s) 15
TfiI GAWTC 1 cut(s) 153
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TscAI CASTG 1 cut(s) 312
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 3 cut(s) 74, 134, 370
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 1 cut(s) 451
TspGWI ACGGA 1 cut(s) 343
TspRI CASTG 1 cut(s) 312
Van91I CCANNNNNTGG 1 cut(s) 118
XagI CCTNNNNNAGG 1 cut(s) 101
XapI RAATTY 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 2 cut(s) 115, 165
XmnI GAANNNNTTC 1 cut(s) 266
XspI CTAG 1 cut(s) 429
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.