AT4G32690

Two-on-two hemoglobin-3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016598
Physical Location & Seq
Forward (+)
15765112 .. 15767106
1995 bp
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UTR
Exon/CDS
Intron
AT4G32690.1

Sequence Viewer

Length: 528 bp
ATGCAATCGCTGCAAGATAAGGCATCGGTGTTAAGCGGCGTCGATCAGGCTGAGGCGTTCGCCATCGACGAGTCCAATCTGTTCGACAAGCTTGGTCTTCAGACATTCATCAACCTATCCACCAATTTCTATACCAGGGTATATGATGACGAAGAAGAATGGTTTCAATCAATATTTTCTAACTCTAACAAAGAAGATGCCATCCAAAACCAGTATGAGTTCTTTGTCCAGCGTATGGGAGGCCCTCCTTTGTATTCTCAGAGAAAAGGTCATCCTGCTTTGATCGGACGTCACCGTCCATTTCCAGTGACCCATCAAGCTGCTGAGAGATGGCTAGAGCACATGCAAAATGCTCTGGACGATTCGGTTGACATTGACCAGGACTCAAAAATCAAAATGATGAAGTTCTTTAGACACACCGCATTTTTCCTTGTGGCTGGAAACGAGCTGAAGAATCAGAATGAGAAACCGAAGCATAAGCCTCAATGTGCTTGTAAACACGCAGCCAATAAACCAGCAGAAGAATAA

Protein Analysis

175

Amino Acids

20.2

Weight (kDa)

5.8

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bac_globin PF01152 26 - 142 6.3e-46 Bacterial-like globin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32690
fragaria_vesca FvH4_2g36290
malus_domestica MD15G1099500.v1.1
prunus_persica Prupe.1G454300_v2.0.a1
pyrus_communis pycom15g09150
rosa_chinensis RchiOBHm_Chr6g0305591
rosa_laevigata RLG00000010874
rosa_multiflora Rmu_sc0001809.1_g000009
rosa_roxburghii Rroxscaffold_7G00162550
rosa_rugosa Rorug06G0342000
rosa_samantha Rh6BG447500 Rh6CG467700 Rh6DG454300
rosa_wichuraiana Rw6G039570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 294
AatII GACGTC 1 cut(s) 292
AccB7I CCANNNNNTGG 1 cut(s) 235
AciI CCGC 2 cut(s) 36, 420
AcuI CTGAAG 2 cut(s) 83, 470
AcyI GRCGYC 2 cut(s) 39, 289
AfiI CCNNNNNNNGG 1 cut(s) 235
AgsI TTSAA 1 cut(s) 167
AjnI CCWGG 2 cut(s) 134, 378
AluBI AGCT 3 cut(s) 91, 320, 448
AluI AGCT 3 cut(s) 91, 320, 448
Alw21I GWGCWC 1 cut(s) 342
AoxI GGCC 1 cut(s) 241
ApeKI GCWGC 3 cut(s) 10, 320, 503
Asp700I GAANNNNTTC 1 cut(s) 162
AspS9I GGNCC 1 cut(s) 242
AsuHPI GGTGA 1 cut(s) 284
BbsI GAAGAC 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 342
BbvCI CCTCAGC 1 cut(s) 51
BbvI GCAGC 2 cut(s) 307, 515
BccI CCATC 4 cut(s) 71, 209, 321, 324
BciT130I CCWGG 2 cut(s) 136, 380
BfaI CTAG 1 cut(s) 335
BisI GCNGC 4 cut(s) 11, 37, 321, 504
BlsI GCNGC 4 cut(s) 12, 38, 322, 505
Bme1390I CCNGG 2 cut(s) 136, 380
BmgT120I GGNCC 1 cut(s) 242
BmrFI CCNGG 2 cut(s) 136, 380
BmsI GCATC 2 cut(s) 32, 187
BpiI GAAGAC 1 cut(s) 89
Bpu10I CCTNAGC 1 cut(s) 51
BsaHI GRCGYC 2 cut(s) 39, 289
BsaJI CCNNGG 1 cut(s) 135
Bsc4I CCNNNNNNNGG 1 cut(s) 235
Bse1I ACTGG 2 cut(s) 211, 305
BseBI CCWGG 2 cut(s) 136, 380
BseDI CCNNGG 1 cut(s) 135
BseGI GGATG 2 cut(s) 201, 271
BseLI CCNNNNNNNGG 1 cut(s) 235
BseMII CTCAG 3 cut(s) 42, 272, 315
BseNI ACTGG 2 cut(s) 211, 305
BseXI GCAGC 2 cut(s) 307, 515
BshFI GGCC 1 cut(s) 243
BsiHKAI GWGCWC 1 cut(s) 342
BslI CCNNNNNNNGG 1 cut(s) 235
BsnI GGCC 1 cut(s) 243
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 2 cut(s) 43, 282
BspACI CCGC 2 cut(s) 36, 420
BspANI GGCC 1 cut(s) 243
BspCNI CTCAG 3 cut(s) 43, 271, 316
BsrI ACTGG 2 cut(s) 211, 305
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 2 cut(s) 43, 282
BssNI GRCGYC 2 cut(s) 39, 289
Bst2UI CCWGG 2 cut(s) 136, 380
Bst4CI ACNGT 1 cut(s) 296
BstACI GRCGYC 2 cut(s) 39, 289
BstAPI GCANNNNNTGC 1 cut(s) 10
BstDEI CTNAG 3 cut(s) 51, 258, 324
BstF5I GGATG 2 cut(s) 201, 271
BstKTI GATC 2 cut(s) 46, 285
BstMBI GATC 2 cut(s) 43, 282
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 2 cut(s) 136, 380
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 134, 378
BstV1I GCAGC 2 cut(s) 307, 515
BstV2I GAAGAC 1 cut(s) 89
BsuRI GGCC 1 cut(s) 243
BtsCI GGATG 2 cut(s) 201, 271
BtsIMutI CAGTG 1 cut(s) 312
Cfr13I GGNCC 1 cut(s) 242
CseI GACGC 1 cut(s) 28
CviAII CATG 1 cut(s) 343
CviJI RGCY 9 cut(s) 50, 91, 243, 320, 334, 437, 448, 481, 506
CviKI_1 RGCY 9 cut(s) 50, 91, 243, 320, 334, 437, 448, 481, 506
DdeI CTNAG 3 cut(s) 51, 258, 324
DpnI GATC 2 cut(s) 45, 284
DpnII GATC 2 cut(s) 43, 282
DrdI GACNNNNNNGTC 1 cut(s) 294
DseDI GACNNNNNNGTC 1 cut(s) 294
Eco57I CTGAAG 2 cut(s) 83, 470
EcoO109I RGGNCCY 1 cut(s) 242
EcoRII CCWGG 2 cut(s) 134, 378
FaeI CATG 1 cut(s) 346
FaiI YATR 7 cut(s) 132, 142, 144, 216, 236, 344, 477
FatI CATG 1 cut(s) 342
Fnu4HI GCNGC 4 cut(s) 11, 37, 321, 504
FokI GGATG 2 cut(s) 188, 258
Fsp4HI GCNGC 4 cut(s) 11, 37, 321, 504
FspBI CTAG 1 cut(s) 335
GluI GCNGC 4 cut(s) 11, 37, 321, 504
HaeIII GGCC 1 cut(s) 243
HgaI GACGC 1 cut(s) 28
Hin1I GRCGYC 2 cut(s) 39, 289
Hin1II CATG 1 cut(s) 346
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 4 cut(s) 71, 362, 383, 454
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 2 cut(s) 370, 497
Hpy188I TCNGA 4 cut(s) 102, 261, 287, 459
Hpy188III TCNNGA 1 cut(s) 356
Hpy8I GTNNAC 2 cut(s) 370, 497
Hpy99I CGWCG 2 cut(s) 44, 71
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 3 cut(s) 4, 13, 346
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 3 cut(s) 51, 258, 324
HpySE526I ACGT 1 cut(s) 289
Hsp92I GRCGYC 2 cut(s) 39, 289
Hsp92II CATG 1 cut(s) 346
Kzo9I GATC 2 cut(s) 43, 282
Lsp1109I GCAGC 2 cut(s) 307, 515
LweI GCATC 2 cut(s) 32, 187
MaeI CTAG 1 cut(s) 335
MaeII ACGT 1 cut(s) 289
MaeIII GTNAC 2 cut(s) 290, 307
MalI GATC 2 cut(s) 45, 284
MboI GATC 2 cut(s) 43, 282
MboII GAAGA 5 cut(s) 89, 164, 167, 206, 463
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 1 cut(s) 124
MlyI GAGTC 2 cut(s) 80, 377
MnlI CCTC 4 cut(s) 46, 233, 255, 492
MroXI GAANNNNTTC 1 cut(s) 162
MseI TTAA 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 136, 380
MvaI CCWGG 2 cut(s) 136, 380
MwoI GCNNNNNNNGC 1 cut(s) 10
NdeII GATC 2 cut(s) 43, 282
NlaIII CATG 1 cut(s) 346
NmuCI GTSAC 2 cut(s) 290, 307
NspI RCATGY 1 cut(s) 346
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PdmI GAANNNNTTC 1 cut(s) 162
PfeI GAWTC 2 cut(s) 362, 454
PflMI CCANNNNNTGG 1 cut(s) 235
PkrI GCNGC 4 cut(s) 12, 38, 322, 505
PleI GAGTC 2 cut(s) 79, 377
PpsI GAGTC 2 cut(s) 79, 377
Psp6I CCWGG 2 cut(s) 134, 378
PspGI CCWGG 2 cut(s) 134, 378
PspPI GGNCC 1 cut(s) 242
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 4 cut(s) 11, 37, 321, 504
Sau3AI GATC 2 cut(s) 43, 282
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 2 cut(s) 80, 377
ScrFI CCNGG 2 cut(s) 136, 380
SduI GDGCHC 1 cut(s) 342
SetI ASST 6 cut(s) 93, 117, 271, 292, 322, 450
SfaNI GCATC 2 cut(s) 32, 187
Sse9I AATT 1 cut(s) 124
SsiI CCGC 2 cut(s) 36, 420
SspI AATATT 1 cut(s) 174
SspMI CTAG 1 cut(s) 335
StyD4I CCNGG 2 cut(s) 134, 378
TaaI ACNGT 1 cut(s) 296
TaiI ACGT 1 cut(s) 292
TaqI TCGA 3 cut(s) 42, 66, 84
TasI AATT 1 cut(s) 124
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 2 cut(s) 362, 454
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TscAI CASTG 1 cut(s) 312
TseFI GTSAC 2 cut(s) 290, 307
TseI GCWGC 3 cut(s) 10, 320, 503
Tsp45I GTSAC 2 cut(s) 290, 307
TspDTI ATGAA 2 cut(s) 97, 416
TspRI CASTG 1 cut(s) 312
Van91I CCANNNNNTGG 1 cut(s) 235
XceI RCATGY 1 cut(s) 346
XmnI GAANNNNTTC 1 cut(s) 162
XspI CTAG 1 cut(s) 335
ZraI GACGTC 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.