Rroxscaffold_7G00162550

Group 2 truncated hemoglobin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016598
Physical Location & Seq
Reverse (-)
5022594 .. 5023931
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00162550.1

Sequence Viewer

Length: 483 bp
ATGCAGAGTCTTCAAGCCAAGGCCTCCGAGTGGAGCGGAGTCGACTCGGCCGACGCGTTCGCCATCGACGACACCAACCTCTTCCAGAAGCTCGGCCTCCAGGCCTTCATTAATCTCTCCACCGATTTCTACACCAGGGTTTACGACGACGAGCAGGAATGGTTCAGGTCAATCTTCGCGAATTCCAAGAAGGAAGACGCGATTCAGAACCAGTACGAGTTTTTCGTGCAGAGAATGGGAGGGCCGTCTCTCTACTCTCAAAGAAAAGGCCATCCTGCTCTGATCGGGCGGCACAGGCCATTTCCGGTGACGCATGAAGCGGCAGAGAGGTGGTTGCATCATATGCAGCAAGCATTGGAGAGCTCTTCAGATATTGACGAGGATTCGAAGCGAAAAATGTTCAATTTCTTCAAGCACACTGCGTTTTTCCTTGTGGCTGGAGATGAGTTGAAGAAGGGTCAAACTCAGAACCCTAAACCCTAA

Protein Analysis

160

Amino Acids

18.51

Weight (kDa)

6.06

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bac_globin PF01152 26 - 142 5.5e-43 Bacterial-like globin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32690
fragaria_vesca FvH4_2g36290
malus_domestica MD15G1099500.v1.1
prunus_persica Prupe.1G454300_v2.0.a1
pyrus_communis pycom15g09150
rosa_chinensis RchiOBHm_Chr6g0305591
rosa_laevigata RLG00000010874
rosa_multiflora Rmu_sc0001809.1_g000009
rosa_roxburghii Rroxscaffold_7G00162550
rosa_rugosa Rorug06G0342000
rosa_samantha Rh6BG447500 Rh6CG467700 Rh6DG454300
rosa_wichuraiana Rw6G039570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 36
AccI GTMKAC 1 cut(s) 42
AccII CGCG 3 cut(s) 56, 179, 200
AciI CCGC 3 cut(s) 36, 289, 320
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 1 cut(s) 181
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 1 cut(s) 30
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 4 cut(s) 14, 403, 412, 451
AjnI CCWGG 2 cut(s) 99, 134
AluBI AGCT 2 cut(s) 91, 363
AluI AGCT 2 cut(s) 91, 363
Alw21I GWGCWC 1 cut(s) 365
Alw26I GTCTC 1 cut(s) 252
AoxI GGCC 7 cut(s) 21, 48, 94, 102, 242, 268, 296
ApeKI GCWGC 1 cut(s) 346
ApoI RAATTY 1 cut(s) 181
AseI ATTAAT 1 cut(s) 111
AspS9I GGNCC 1 cut(s) 242
AsuHPI GGTGA 1 cut(s) 319
AsuII TTCGAA 1 cut(s) 386
BanII GRGCYC 1 cut(s) 365
BbsI GAAGAC 2 cut(s) 2, 201
Bbv12I GWGCWC 1 cut(s) 365
BbvI GCAGC 1 cut(s) 358
BccI CCATC 2 cut(s) 71, 279
BceAI ACGGC 1 cut(s) 229
BciT130I CCWGG 2 cut(s) 101, 136
BcoDI GTCTC 1 cut(s) 252
BisI GCNGC 3 cut(s) 290, 321, 347
BlsI GCNGC 3 cut(s) 291, 322, 348
Bme1390I CCNGG 2 cut(s) 101, 136
BmgT120I GGNCC 1 cut(s) 242
BmrFI CCNGG 2 cut(s) 101, 136
BmsI GCATC 1 cut(s) 346
BpiI GAAGAC 2 cut(s) 2, 201
BpmI CTGGAG 2 cut(s) 83, 459
Bpu14I TTCGAA 1 cut(s) 386
BsaJI CCNNGG 2 cut(s) 18, 135
BsaWI WCCGGW 1 cut(s) 304
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse1I ACTGG 1 cut(s) 211
BseBI CCWGG 2 cut(s) 101, 136
BseDI CCNNGG 2 cut(s) 18, 135
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMII CTCAG 1 cut(s) 479
BseNI ACTGG 1 cut(s) 211
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 358
BsgI GTGCAG 1 cut(s) 248
Bsh1236I CGCG 3 cut(s) 56, 179, 200
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 7 cut(s) 23, 50, 96, 104, 244, 270, 298
BsiEI CGRYCG 1 cut(s) 51
BsiHKAI GWGCWC 1 cut(s) 365
BsiSI CCGG 1 cut(s) 305
BslI CCNNNNNNNGG 1 cut(s) 30
BsmAI GTCTC 1 cut(s) 252
BsmBI CGTCTC 1 cut(s) 252
BsnI GGCC 7 cut(s) 23, 50, 96, 104, 244, 270, 298
Bsp119I TTCGAA 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 365
Bsp143I GATC 1 cut(s) 282
Bsp68I TCGCGA 1 cut(s) 179
BspACI CCGC 3 cut(s) 36, 289, 320
BspANI GGCC 7 cut(s) 23, 50, 96, 104, 244, 270, 298
BspCNI CTCAG 1 cut(s) 478
BspFNI CGCG 3 cut(s) 56, 179, 200
BspQI GCTCTTC 1 cut(s) 370
BspT104I TTCGAA 1 cut(s) 386
BsrBI CCGCTC 1 cut(s) 36
BsrI ACTGG 1 cut(s) 211
BssECI CCNNGG 2 cut(s) 18, 135
BssMI GATC 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 101, 136
Bst6I CTCTTC 2 cut(s) 86, 370
BstAPI GCANNNNNTGC 1 cut(s) 343
BstBI TTCGAA 1 cut(s) 386
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 1 cut(s) 465
BstF5I GGATG 1 cut(s) 271
BstFNI CGCG 3 cut(s) 56, 179, 200
BstKTI GATC 1 cut(s) 285
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 1 cut(s) 282
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 2 cut(s) 295, 343
BstNI CCWGG 2 cut(s) 101, 136
BstSCI CCNGG 2 cut(s) 99, 134
BstUI CGCG 3 cut(s) 56, 179, 200
BstV1I GCAGC 1 cut(s) 358
BstV2I GAAGAC 2 cut(s) 2, 201
BstZI CGGCCG 1 cut(s) 48
BsuRI GGCC 7 cut(s) 23, 50, 96, 104, 244, 270, 298
BtsCI GGATG 1 cut(s) 271
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 1 cut(s) 417
BtuMI TCGCGA 1 cut(s) 179
Cac8I GCNNGC 1 cut(s) 351
Cfr13I GGNCC 1 cut(s) 242
CseI GACGC 3 cut(s) 62, 206, 319
Csp6I GTAC 1 cut(s) 214
CviAII CATG 1 cut(s) 314
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 1 cut(s) 465
DpnI GATC 1 cut(s) 284
DpnII GATC 1 cut(s) 282
EaeI YGGCCR 1 cut(s) 48
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 2 cut(s) 86, 370
EarI CTCTTC 2 cut(s) 86, 370
Ecl136II GAGCTC 1 cut(s) 363
EclXI CGGCCG 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 18
Eco147I AGGCCT 2 cut(s) 23, 104
Eco24I GRGCYC 1 cut(s) 365
Eco52I CGGCCG 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 363
Eco57I CTGAAG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 363
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 2 cut(s) 99, 134
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 1 cut(s) 365
ErhI CCWWGG 1 cut(s) 18
Esp3I CGTCTC 1 cut(s) 252
FaeI CATG 1 cut(s) 317
FaiI YATR 3 cut(s) 315, 342, 344
FatI CATG 1 cut(s) 313
FauNDI CATATG 1 cut(s) 342
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 3 cut(s) 290, 321, 347
FokI GGATG 1 cut(s) 258
FriOI GRGCYC 1 cut(s) 365
Fsp4HI GCNGC 3 cut(s) 290, 321, 347
GluI GCNGC 3 cut(s) 290, 321, 347
GsuI CTGGAG 2 cut(s) 83, 459
HaeIII GGCC 7 cut(s) 23, 50, 96, 104, 244, 270, 298
HapII CCGG 1 cut(s) 305
HgaI GACGC 3 cut(s) 62, 206, 319
Hin1II CATG 1 cut(s) 317
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 5 cut(s) 7, 39, 44, 202, 383
HpaII CCGG 1 cut(s) 305
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 2 cut(s) 43, 142
Hpy188I TCNGA 5 cut(s) 28, 207, 282, 370, 468
Hpy188III TCNNGA 2 cut(s) 85, 178
Hpy8I GTNNAC 2 cut(s) 43, 142
Hpy99I CGWCG 4 cut(s) 56, 71, 149, 152
HpyAV CCTTC 3 cut(s) 115, 184, 448
HpyCH4V TGCA 4 cut(s) 4, 229, 337, 346
HpyF10VI GCNNNNNNNGC 2 cut(s) 295, 343
HpyF3I CTNAG 1 cut(s) 465
Hsp92II CATG 1 cut(s) 317
Kzo9I GATC 1 cut(s) 282
LguI GCTCTTC 1 cut(s) 370
LmnI GCTCC 1 cut(s) 33
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 1 cut(s) 346
MaeIII GTNAC 1 cut(s) 307
MalI GATC 1 cut(s) 284
MbiI CCGCTC 1 cut(s) 36
MboI GATC 1 cut(s) 282
MboII GAAGA 6 cut(s) 73, 166, 206, 357, 400, 463
MhlI GDGCHC 1 cut(s) 365
MluCI AATT 2 cut(s) 181, 403
MluI ACGCGT 1 cut(s) 54
MlyI GAGTC 3 cut(s) 16, 38, 48
MnlI CCTC 6 cut(s) 34, 89, 107, 233, 321, 373
MseI TTAA 1 cut(s) 111
MspI CCGG 1 cut(s) 305
MspR9I CCNGG 2 cut(s) 101, 136
MvaI CCWGG 2 cut(s) 101, 136
MvnI CGCG 3 cut(s) 56, 179, 200
MwoI GCNNNNNNNGC 2 cut(s) 295, 343
NdeI CATATG 1 cut(s) 342
NdeII GATC 1 cut(s) 282
NlaIII CATG 1 cut(s) 317
NmeAIII GCCGAG 2 cut(s) 26, 72
NmuCI GTSAC 1 cut(s) 307
NruI TCGCGA 1 cut(s) 179
NspV TTCGAA 1 cut(s) 386
PceI AGGCCT 2 cut(s) 23, 104
PciSI GCTCTTC 1 cut(s) 370
PcsI WCGNNNNNNNCGW 4 cut(s) 48, 53, 66, 222
PfeI GAWTC 2 cut(s) 202, 383
PkrI GCNGC 3 cut(s) 291, 322, 348
PleI GAGTC 3 cut(s) 15, 38, 47
PpsI GAGTC 3 cut(s) 15, 38, 47
PshBI ATTAAT 1 cut(s) 111
Psp124BI GAGCTC 1 cut(s) 365
Psp6I CCWGG 2 cut(s) 99, 134
PspGI CCWGG 2 cut(s) 99, 134
PspPI GGNCC 1 cut(s) 242
RruI TCGCGA 1 cut(s) 179
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SacI GAGCTC 1 cut(s) 365
SalI GTCGAC 1 cut(s) 41
SapI GCTCTTC 1 cut(s) 370
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 3 cut(s) 290, 321, 347
Sau3AI GATC 1 cut(s) 282
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 3 cut(s) 16, 38, 48
ScrFI CCNGG 2 cut(s) 101, 136
SduI GDGCHC 1 cut(s) 365
SetI ASST 5 cut(s) 81, 93, 170, 332, 365
SfaNI GCATC 1 cut(s) 346
SfuI TTCGAA 1 cut(s) 386
Sse9I AATT 2 cut(s) 181, 403
SseBI AGGCCT 2 cut(s) 23, 104
SsiI CCGC 3 cut(s) 36, 289, 320
SstI GAGCTC 1 cut(s) 365
StuI AGGCCT 2 cut(s) 23, 104
StyD4I CCNGG 2 cut(s) 99, 134
StyI CCWWGG 1 cut(s) 18
TaqI TCGA 3 cut(s) 42, 66, 386
TasI AATT 2 cut(s) 181, 403
TauI GCSGC 2 cut(s) 292, 323
TfiI GAWTC 2 cut(s) 202, 383
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 1 cut(s) 424
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 1 cut(s) 346
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 2 cut(s) 97, 330
TspRI CASTG 1 cut(s) 424
VspI ATTAAT 1 cut(s) 111
XapI RAATTY 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.