Rmu_sc0001809.1_g000009

Group 2 truncated hemoglobin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001809.1
OG0016598
Physical Location & Seq
Forward (+)
59154 .. 59802
649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001809.1_g000009.1.cds

Sequence Viewer

Length: 564 bp
atgcagggtcttcaagccaaggcctccgagtggagcggagtcgactcggccgacgcgttcgccatcgacgacaccaacctcttcgagaagctcggcctccaggccttcgttaatctctccaccgatttctacaccaggtttggtttcagtcttctctttcctttgatcatcgatcgatatcggaatgaattgaatttacaattgagccttcacgccagggtttacgacgacgagcaggaatggttcaggtcaatcttcgcgaattccaagaaggaagacgcgattcagaatcagtacgagtttttcgtgcagagaatgggagggccgtctctctactctcaaagaaaaggccatcctgctctgatcgggcggcacaggccatttccggtgacgcatgaagcggcagagaggtggttgcatcatatgcagcaagcattggagagctcttcagatattgacgaggattcgaagcgaaaaatgttcaatttcttcaagcacactgcgtttttccttgtggctggagacgagttgaagaagggtcaaactcagaaccctaaaccctaa

Protein Analysis

187

Amino Acids

21.7

Weight (kDa)

6.16

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32690
fragaria_vesca FvH4_2g36290
malus_domestica MD15G1099500.v1.1
prunus_persica Prupe.1G454300_v2.0.a1
pyrus_communis pycom15g09150
rosa_chinensis RchiOBHm_Chr6g0305591
rosa_laevigata RLG00000010874
rosa_multiflora Rmu_sc0001809.1_g000009
rosa_roxburghii Rroxscaffold_7G00162550
rosa_rugosa Rorug06G0342000
rosa_samantha Rh6BG447500 Rh6CG467700 Rh6DG454300
rosa_wichuraiana Rw6G039570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 36
AccI GTMKAC 1 cut(s) 42
AccII CGCG 3 cut(s) 56, 260, 281
AciI CCGC 3 cut(s) 36, 370, 401
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 2 cut(s) 193, 262
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 1 cut(s) 296
AfiI CCNNNNNNNGG 1 cut(s) 30
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 5 cut(s) 14, 193, 484, 493, 532
AjnI CCWGG 3 cut(s) 99, 134, 215
AluBI AGCT 2 cut(s) 91, 444
AluI AGCT 2 cut(s) 91, 444
Alw21I GWGCWC 1 cut(s) 446
Alw26I GTCTC 2 cut(s) 333, 516
AoxI GGCC 7 cut(s) 21, 48, 94, 102, 323, 349, 377
ApeKI GCWGC 1 cut(s) 427
ApoI RAATTY 2 cut(s) 193, 262
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 400
AsuII TTCGAA 1 cut(s) 467
BanII GRGCYC 1 cut(s) 446
BbsI GAAGAC 3 cut(s) 2, 143, 282
Bbv12I GWGCWC 1 cut(s) 446
BbvI GCAGC 1 cut(s) 439
BccI CCATC 2 cut(s) 71, 360
BceAI ACGGC 1 cut(s) 310
BciT130I CCWGG 3 cut(s) 101, 136, 217
BclI TGATCA 1 cut(s) 165
BcoDI GTCTC 2 cut(s) 333, 516
BisI GCNGC 3 cut(s) 371, 402, 428
BlsI GCNGC 3 cut(s) 372, 403, 429
Bme1390I CCNGG 3 cut(s) 101, 136, 217
BmgT120I GGNCC 1 cut(s) 323
BmrFI CCNGG 3 cut(s) 101, 136, 217
BmsI GCATC 1 cut(s) 427
BpiI GAAGAC 3 cut(s) 2, 143, 282
BpmI CTGGAG 2 cut(s) 83, 540
Bpu14I TTCGAA 1 cut(s) 467
Bsa29I ATCGAT 2 cut(s) 171, 175
BsaBI GATNNNNATC 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 18, 216
BsaWI WCCGGW 1 cut(s) 385
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse8I GATNNNNATC 1 cut(s) 177
BseBI CCWGG 3 cut(s) 101, 136, 217
BseCI ATCGAT 2 cut(s) 171, 175
BseDI CCNNGG 2 cut(s) 18, 216
BseGI GGATG 1 cut(s) 352
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMII CTCAG 1 cut(s) 560
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 439
BsgI GTGCAG 1 cut(s) 329
Bsh1236I CGCG 3 cut(s) 56, 260, 281
Bsh1285I CGRYCG 2 cut(s) 51, 175
BshFI GGCC 7 cut(s) 23, 50, 96, 104, 325, 351, 379
BshVI ATCGAT 2 cut(s) 171, 175
BsiEI CGRYCG 2 cut(s) 51, 175
BsiHKAI GWGCWC 1 cut(s) 446
BsiSI CCGG 1 cut(s) 386
BslI CCNNNNNNNGG 1 cut(s) 30
BsmAI GTCTC 2 cut(s) 333, 516
BsmBI CGTCTC 2 cut(s) 333, 516
BsnI GGCC 7 cut(s) 23, 50, 96, 104, 325, 351, 379
Bsp119I TTCGAA 1 cut(s) 467
Bsp1286I GDGCHC 1 cut(s) 446
Bsp143I GATC 3 cut(s) 165, 172, 363
Bsp68I TCGCGA 1 cut(s) 260
BspACI CCGC 3 cut(s) 36, 370, 401
BspANI GGCC 7 cut(s) 23, 50, 96, 104, 325, 351, 379
BspCNI CTCAG 1 cut(s) 559
BspDI ATCGAT 2 cut(s) 171, 175
BspFNI CGCG 3 cut(s) 56, 260, 281
BspQI GCTCTTC 1 cut(s) 451
BspT104I TTCGAA 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 36
BssECI CCNNGG 2 cut(s) 18, 216
BssMI GATC 3 cut(s) 165, 172, 363
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 3 cut(s) 101, 136, 217
Bst6I CTCTTC 2 cut(s) 86, 451
BstAPI GCANNNNNTGC 1 cut(s) 424
BstBI TTCGAA 1 cut(s) 467
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 1 cut(s) 546
BstF5I GGATG 1 cut(s) 352
BstFNI CGCG 3 cut(s) 56, 260, 281
BstKTI GATC 3 cut(s) 168, 175, 366
BstMAI GTCTC 2 cut(s) 333, 516
BstMBI GATC 3 cut(s) 165, 172, 363
BstMCI CGRYCG 2 cut(s) 51, 175
BstMWI GCNNNNNNNGC 2 cut(s) 376, 424
BstNI CCWGG 3 cut(s) 101, 136, 217
BstSCI CCNGG 3 cut(s) 99, 134, 215
BstUI CGCG 3 cut(s) 56, 260, 281
BstV1I GCAGC 1 cut(s) 439
BstV2I GAAGAC 3 cut(s) 2, 143, 282
BstZI CGGCCG 1 cut(s) 48
Bsu15I ATCGAT 2 cut(s) 171, 175
BsuRI GGCC 7 cut(s) 23, 50, 96, 104, 325, 351, 379
BsuTUI ATCGAT 2 cut(s) 171, 175
BtsCI GGATG 1 cut(s) 352
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 1 cut(s) 498
BtuMI TCGCGA 1 cut(s) 260
Cac8I GCNNGC 1 cut(s) 432
Cfr13I GGNCC 1 cut(s) 323
ClaI ATCGAT 2 cut(s) 171, 175
CseI GACGC 3 cut(s) 62, 287, 400
CsiI ACCWGGT 1 cut(s) 134
Csp6I GTAC 1 cut(s) 295
CviAII CATG 1 cut(s) 395
CviQI GTAC 1 cut(s) 295
DdeI CTNAG 1 cut(s) 546
DpnI GATC 3 cut(s) 167, 174, 365
DpnII GATC 3 cut(s) 165, 172, 363
EaeI YGGCCR 1 cut(s) 48
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 2 cut(s) 86, 451
EarI CTCTTC 2 cut(s) 86, 451
Ecl136II GAGCTC 1 cut(s) 444
EclXI CGGCCG 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 18
Eco147I AGGCCT 2 cut(s) 23, 104
Eco24I GRGCYC 1 cut(s) 446
Eco32I GATATC 1 cut(s) 179
Eco52I CGGCCG 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 444
Eco57I CTGAAG 1 cut(s) 432
EcoICRI GAGCTC 1 cut(s) 444
EcoRI GAATTC 1 cut(s) 262
EcoRII CCWGG 3 cut(s) 99, 134, 215
EcoRV GATATC 1 cut(s) 179
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 1 cut(s) 446
ErhI CCWWGG 1 cut(s) 18
Esp3I CGTCTC 2 cut(s) 333, 516
FaeI CATG 1 cut(s) 398
FaiI YATR 3 cut(s) 396, 423, 425
FatI CATG 1 cut(s) 394
FauNDI CATATG 1 cut(s) 423
FbaI TGATCA 1 cut(s) 165
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 3 cut(s) 371, 402, 428
FokI GGATG 1 cut(s) 339
FriOI GRGCYC 1 cut(s) 446
Fsp4HI GCNGC 3 cut(s) 371, 402, 428
GluI GCNGC 3 cut(s) 371, 402, 428
GsuI CTGGAG 2 cut(s) 83, 540
HaeIII GGCC 7 cut(s) 23, 50, 96, 104, 325, 351, 379
HapII CCGG 1 cut(s) 386
HgaI GACGC 3 cut(s) 62, 287, 400
Hin1II CATG 1 cut(s) 398
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 5 cut(s) 39, 44, 283, 289, 464
HpaII CCGG 1 cut(s) 386
HphI GGTGA 1 cut(s) 400
Hpy166II GTNNAC 2 cut(s) 43, 223
Hpy188I TCNGA 6 cut(s) 28, 183, 288, 363, 451, 549
Hpy188III TCNNGA 2 cut(s) 85, 259
Hpy8I GTNNAC 2 cut(s) 43, 223
Hpy99I CGWCG 4 cut(s) 56, 71, 230, 233
HpyAV CCTTC 4 cut(s) 115, 218, 265, 529
HpyCH4V TGCA 4 cut(s) 4, 310, 418, 427
HpyF10VI GCNNNNNNNGC 2 cut(s) 376, 424
HpyF3I CTNAG 1 cut(s) 546
Hsp92II CATG 1 cut(s) 398
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 3 cut(s) 165, 172, 363
LguI GCTCTTC 1 cut(s) 451
LmnI GCTCC 1 cut(s) 33
Lsp1109I GCAGC 1 cut(s) 439
LweI GCATC 1 cut(s) 427
MabI ACCWGGT 1 cut(s) 134
MaeIII GTNAC 1 cut(s) 388
MalI GATC 3 cut(s) 167, 174, 365
MbiI CCGCTC 1 cut(s) 36
MboI GATC 3 cut(s) 165, 172, 363
MboII GAAGA 7 cut(s) 73, 143, 247, 287, 438, 481, 544
MfeI CAATTG 1 cut(s) 200
MhlI GDGCHC 1 cut(s) 446
MluCI AATT 5 cut(s) 188, 193, 200, 262, 484
MluI ACGCGT 1 cut(s) 54
MlyI GAGTC 2 cut(s) 38, 48
MnlI CCTC 6 cut(s) 34, 89, 107, 314, 402, 454
MseI TTAA 1 cut(s) 111
MspI CCGG 1 cut(s) 386
MspR9I CCNGG 3 cut(s) 101, 136, 217
MunI CAATTG 1 cut(s) 200
MvaI CCWGG 3 cut(s) 101, 136, 217
MvnI CGCG 3 cut(s) 56, 260, 281
MwoI GCNNNNNNNGC 2 cut(s) 376, 424
NdeI CATATG 1 cut(s) 423
NdeII GATC 3 cut(s) 165, 172, 363
NlaIII CATG 1 cut(s) 398
NmeAIII GCCGAG 2 cut(s) 26, 72
NmuCI GTSAC 1 cut(s) 388
NruI TCGCGA 1 cut(s) 260
NspV TTCGAA 1 cut(s) 467
PceI AGGCCT 2 cut(s) 23, 104
PciSI GCTCTTC 1 cut(s) 451
PcsI WCGNNNNNNNCGW 4 cut(s) 48, 53, 66, 303
PfeI GAWTC 3 cut(s) 283, 289, 464
PkrI GCNGC 3 cut(s) 372, 403, 429
Ple19I CGATCG 1 cut(s) 175
PleI GAGTC 2 cut(s) 38, 47
PpsI GAGTC 2 cut(s) 38, 47
Psp124BI GAGCTC 1 cut(s) 446
Psp6I CCWGG 3 cut(s) 99, 134, 215
PspGI CCWGG 3 cut(s) 99, 134, 215
PspPI GGNCC 1 cut(s) 323
PvuI CGATCG 1 cut(s) 175
RruI TCGCGA 1 cut(s) 260
RsaI GTAC 1 cut(s) 296
RsaNI GTAC 1 cut(s) 295
SacI GAGCTC 1 cut(s) 446
SalI GTCGAC 1 cut(s) 41
SapI GCTCTTC 1 cut(s) 451
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 3 cut(s) 371, 402, 428
Sau3AI GATC 3 cut(s) 165, 172, 363
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 2 cut(s) 38, 48
ScrFI CCNGG 3 cut(s) 101, 136, 217
SduI GDGCHC 1 cut(s) 446
SetI ASST 6 cut(s) 81, 93, 140, 251, 413, 446
SexAI ACCWGGT 1 cut(s) 134
SfaNI GCATC 1 cut(s) 427
SfuI TTCGAA 1 cut(s) 467
Sse9I AATT 5 cut(s) 188, 193, 200, 262, 484
SseBI AGGCCT 2 cut(s) 23, 104
SsiI CCGC 3 cut(s) 36, 370, 401
SstI GAGCTC 1 cut(s) 446
StuI AGGCCT 2 cut(s) 23, 104
StyD4I CCNGG 3 cut(s) 99, 134, 215
StyI CCWWGG 1 cut(s) 18
TaqI TCGA 6 cut(s) 42, 66, 84, 171, 175, 467
TasI AATT 5 cut(s) 188, 193, 200, 262, 484
TauI GCSGC 2 cut(s) 373, 404
TfiI GAWTC 3 cut(s) 283, 289, 464
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 1 cut(s) 505
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 1 cut(s) 427
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 2 cut(s) 201, 411
TspRI CASTG 1 cut(s) 505
XapI RAATTY 2 cut(s) 193, 262
XmiI GTMKAC 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.