FvH4_2g36290

Group 2 truncated hemoglobin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016598
Physical Location & Seq
Reverse (-)
26602865 .. 26603587
723 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36290.t1

Sequence Viewer

Length: 504 bp
ATGCAAAGTCTTCAAGCCAAGGCCTCCCAGTGGAGCGGAGTCGACTCCGCCGACGCGTTCGCCATCGACGAAACCAACCTCTTCCAGAAGCTTGGTCTCCAAGCCTTCATCAATCTCTCCACCGATTTCTACACCAGGGTTTACGAGGACGAGCAGGAATGGTTCCGATCAATCTTCGCGAATTCCAAGAAGGAAGACGCGATTCAGAACCAGTACGAGTTTTTCGTGCAGAGAATGGGAGGGCCGCCTCTCTTTTCTCAAAGAAGAGGCCATCCTGCACTGATCGGACGGCACCGGCCGTTTCCGGTGACGCATGAAGCAGCGGAGAGGTGGTTGCATCACATGCAGCAAGCATTGGAGAGCTCTTCAGATATTGATGAGGATTCGAAGCGAAGAATGTTCAATTTCTTCAAGCATACTGCTTATTTCCTTGTGGCTGGAGATGAGTTGAAGAAGGGTCACACTCAGAATGGGACTCAAACTCAGACCCAACAACAACAATAA

Protein Analysis

168

Amino Acids

19.42

Weight (kDa)

6.15

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bac_globin PF01152 26 - 142 1.3e-42 Bacterial-like globin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32690
fragaria_vesca FvH4_2g36290
malus_domestica MD15G1099500.v1.1
prunus_persica Prupe.1G454300_v2.0.a1
pyrus_communis pycom15g09150
rosa_chinensis RchiOBHm_Chr6g0305591
rosa_laevigata RLG00000010874
rosa_multiflora Rmu_sc0001809.1_g000009
rosa_roxburghii Rroxscaffold_7G00162550
rosa_rugosa Rorug06G0342000
rosa_samantha Rh6BG447500 Rh6CG467700 Rh6DG454300
rosa_wichuraiana Rw6G039570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 291
AccBSI CCGCTC 1 cut(s) 36
AccI GTMKAC 1 cut(s) 42
AccII CGCG 3 cut(s) 56, 179, 200
AciI CCGC 4 cut(s) 36, 48, 245, 323
AcoI YGGCCR 1 cut(s) 296
AcsI RAATTY 1 cut(s) 181
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 1 cut(s) 30
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 4 cut(s) 14, 403, 412, 451
AjnI CCWGG 1 cut(s) 134
AluBI AGCT 2 cut(s) 91, 363
AluI AGCT 2 cut(s) 91, 363
Alw21I GWGCWC 1 cut(s) 365
Alw26I GTCTC 1 cut(s) 101
AoxI GGCC 4 cut(s) 21, 242, 268, 296
ApeKI GCWGC 2 cut(s) 320, 346
ApoI RAATTY 1 cut(s) 181
AspS9I GGNCC 1 cut(s) 242
AsuHPI GGTGA 1 cut(s) 319
AsuII TTCGAA 1 cut(s) 386
BanI GGYRCC 1 cut(s) 291
BanII GRGCYC 1 cut(s) 365
BbsI GAAGAC 2 cut(s) 2, 201
Bbv12I GWGCWC 1 cut(s) 365
BbvI GCAGC 2 cut(s) 332, 358
BccI CCATC 2 cut(s) 71, 279
BceAI ACGGC 2 cut(s) 283, 305
BciT130I CCWGG 1 cut(s) 136
BcoDI GTCTC 1 cut(s) 101
BisI GCNGC 3 cut(s) 245, 321, 347
BlsI GCNGC 3 cut(s) 246, 322, 348
Bme1390I CCNGG 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 2 cut(s) 164, 293
BmrFI CCNGG 1 cut(s) 136
BmrI ACTGGG 1 cut(s) 22
BmsI GCATC 1 cut(s) 346
BmuI ACTGGG 1 cut(s) 22
BpiI GAAGAC 2 cut(s) 2, 201
BpmI CTGGAG 1 cut(s) 459
Bpu14I TTCGAA 1 cut(s) 386
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 18, 135
BsaWI WCCGGW 1 cut(s) 304
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse118I RCCGGY 1 cut(s) 294
Bse1I ACTGG 2 cut(s) 28, 211
BseBI CCWGG 1 cut(s) 136
BseDI CCNNGG 2 cut(s) 18, 135
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMII CTCAG 2 cut(s) 479, 497
BseNI ACTGG 2 cut(s) 28, 211
BseX3I CGGCCG 1 cut(s) 296
BseXI GCAGC 2 cut(s) 332, 358
BsgI GTGCAG 2 cut(s) 248, 261
Bsh1236I CGCG 3 cut(s) 56, 179, 200
Bsh1285I CGRYCG 1 cut(s) 299
BshFI GGCC 4 cut(s) 23, 244, 270, 298
BshNI GGYRCC 1 cut(s) 291
BsiEI CGRYCG 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 365
BsiSI CCGG 2 cut(s) 295, 305
BslFI GGGAC 1 cut(s) 487
BslI CCNNNNNNNGG 1 cut(s) 30
BsmAI GTCTC 1 cut(s) 101
BsmFI GGGAC 1 cut(s) 487
BsnI GGCC 4 cut(s) 23, 244, 270, 298
Bso31I GGTCTC 1 cut(s) 101
Bsp119I TTCGAA 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 365
Bsp143I GATC 2 cut(s) 167, 282
Bsp68I TCGCGA 1 cut(s) 179
BspACI CCGC 4 cut(s) 36, 48, 245, 323
BspANI GGCC 4 cut(s) 23, 244, 270, 298
BspCNI CTCAG 2 cut(s) 478, 496
BspFNI CGCG 3 cut(s) 56, 179, 200
BspLI GGNNCC 2 cut(s) 164, 293
BspQI GCTCTTC 1 cut(s) 370
BspT104I TTCGAA 1 cut(s) 386
BspT107I GGYRCC 1 cut(s) 291
BspTNI GGTCTC 1 cut(s) 101
BsrBI CCGCTC 1 cut(s) 36
BsrFI RCCGGY 1 cut(s) 294
BsrI ACTGG 2 cut(s) 28, 211
BssAI RCCGGY 1 cut(s) 294
BssECI CCNNGG 2 cut(s) 18, 135
BssMI GATC 2 cut(s) 167, 282
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 136
Bst6I CTCTTC 3 cut(s) 86, 259, 370
BstAPI GCANNNNNTGC 1 cut(s) 343
BstBI TTCGAA 1 cut(s) 386
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 2 cut(s) 465, 483
BstF5I GGATG 1 cut(s) 271
BstFNI CGCG 3 cut(s) 56, 179, 200
BstKTI GATC 2 cut(s) 170, 285
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 2 cut(s) 167, 282
BstMCI CGRYCG 1 cut(s) 299
BstMWI GCNNNNNNNGC 1 cut(s) 343
BstNI CCWGG 1 cut(s) 136
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 1 cut(s) 134
BstUI CGCG 3 cut(s) 56, 179, 200
BstV1I GCAGC 2 cut(s) 332, 358
BstV2I GAAGAC 2 cut(s) 2, 201
BstXI CCANNNNNNTGG 1 cut(s) 92
BstZI CGGCCG 1 cut(s) 296
BsuRI GGCC 4 cut(s) 23, 244, 270, 298
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 35, 278
BtuMI TCGCGA 1 cut(s) 179
Cac8I GCNNGC 1 cut(s) 351
Cfr10I RCCGGY 1 cut(s) 294
Cfr13I GGNCC 1 cut(s) 242
CseI GACGC 3 cut(s) 62, 206, 319
Csp6I GTAC 1 cut(s) 214
CviAII CATG 2 cut(s) 314, 343
CviJI RGCY 9 cut(s) 17, 23, 91, 104, 244, 270, 298, 363, 437
CviKI_1 RGCY 9 cut(s) 17, 23, 91, 104, 244, 270, 298, 363, 437
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 2 cut(s) 465, 483
DpnI GATC 2 cut(s) 169, 284
DpnII GATC 2 cut(s) 167, 282
EaeI YGGCCR 1 cut(s) 296
EagI CGGCCG 1 cut(s) 296
Eam1104I CTCTTC 3 cut(s) 86, 259, 370
EarI CTCTTC 3 cut(s) 86, 259, 370
EciI GGCGGA 1 cut(s) 37
Ecl136II GAGCTC 1 cut(s) 363
EclXI CGGCCG 1 cut(s) 296
Eco130I CCWWGG 1 cut(s) 18
Eco147I AGGCCT 1 cut(s) 23
Eco24I GRGCYC 1 cut(s) 365
Eco31I GGTCTC 1 cut(s) 101
Eco52I CGGCCG 1 cut(s) 296
Eco53kI GAGCTC 1 cut(s) 363
Eco57I CTGAAG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 363
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 134
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 1 cut(s) 365
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 2 cut(s) 317, 346
FaiI YATR 3 cut(s) 315, 344, 417
FaqI GGGAC 1 cut(s) 487
FatI CATG 2 cut(s) 313, 342
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 3 cut(s) 245, 321, 347
FokI GGATG 1 cut(s) 258
FriOI GRGCYC 1 cut(s) 365
Fsp4HI GCNGC 3 cut(s) 245, 321, 347
GluI GCNGC 3 cut(s) 245, 321, 347
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 4 cut(s) 23, 244, 270, 298
HapII CCGG 2 cut(s) 295, 305
HgaI GACGC 3 cut(s) 62, 206, 319
Hin1II CATG 2 cut(s) 317, 346
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 5 cut(s) 39, 44, 202, 383, 475
HpaII CCGG 2 cut(s) 295, 305
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 2 cut(s) 43, 142
Hpy188I TCNGA 6 cut(s) 167, 207, 287, 370, 468, 486
Hpy188III TCNNGA 2 cut(s) 85, 178
Hpy8I GTNNAC 2 cut(s) 43, 142
Hpy99I CGWCG 2 cut(s) 56, 71
HpyAV CCTTC 3 cut(s) 115, 184, 448
HpyCH4V TGCA 5 cut(s) 4, 229, 278, 337, 346
HpyF10VI GCNNNNNNNGC 1 cut(s) 343
HpyF3I CTNAG 2 cut(s) 465, 483
Hsp92II CATG 2 cut(s) 317, 346
Kzo9I GATC 2 cut(s) 167, 282
LguI GCTCTTC 1 cut(s) 370
LmnI GCTCC 1 cut(s) 33
Lsp1109I GCAGC 2 cut(s) 332, 358
LweI GCATC 1 cut(s) 346
MaeIII GTNAC 2 cut(s) 307, 458
MalI GATC 2 cut(s) 169, 284
MbiI CCGCTC 1 cut(s) 36
MboI GATC 2 cut(s) 167, 282
MboII GAAGA 8 cut(s) 73, 166, 206, 276, 357, 400, 405, 463
MhlI GDGCHC 1 cut(s) 365
MluCI AATT 2 cut(s) 181, 403
MluI ACGCGT 1 cut(s) 54
MlyI GAGTC 3 cut(s) 38, 48, 469
MnlI CCTC 8 cut(s) 34, 89, 139, 233, 258, 260, 321, 373
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 2 cut(s) 295, 305
MspR9I CCNGG 1 cut(s) 136
MvaI CCWGG 1 cut(s) 136
MvnI CGCG 3 cut(s) 56, 179, 200
MwoI GCNNNNNNNGC 1 cut(s) 343
NdeII GATC 2 cut(s) 167, 282
NlaIII CATG 2 cut(s) 317, 346
NlaIV GGNNCC 2 cut(s) 164, 293
NmuCI GTSAC 2 cut(s) 307, 458
NruI TCGCGA 1 cut(s) 179
NspI RCATGY 1 cut(s) 346
NspV TTCGAA 1 cut(s) 386
PceI AGGCCT 1 cut(s) 23
PciSI GCTCTTC 1 cut(s) 370
PcsI WCGNNNNNNNCGW 3 cut(s) 48, 66, 222
PfeI GAWTC 2 cut(s) 202, 383
PkrI GCNGC 3 cut(s) 246, 322, 348
PleI GAGTC 3 cut(s) 38, 47, 469
PpsI GAGTC 3 cut(s) 38, 47, 469
Psp124BI GAGCTC 1 cut(s) 365
Psp6I CCWGG 1 cut(s) 134
PspGI CCWGG 1 cut(s) 134
PspN4I GGNNCC 2 cut(s) 164, 293
PspPI GGNCC 1 cut(s) 242
RruI TCGCGA 1 cut(s) 179
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SacI GAGCTC 1 cut(s) 365
SalI GTCGAC 1 cut(s) 41
SapI GCTCTTC 1 cut(s) 370
SatI GCNGC 3 cut(s) 245, 321, 347
Sau3AI GATC 2 cut(s) 167, 282
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 3 cut(s) 38, 48, 469
ScrFI CCNGG 1 cut(s) 136
SduI GDGCHC 1 cut(s) 365
SetI ASST 4 cut(s) 81, 93, 332, 365
SfaNI GCATC 1 cut(s) 346
SfuI TTCGAA 1 cut(s) 386
Sse9I AATT 2 cut(s) 181, 403
SseBI AGGCCT 1 cut(s) 23
SsiI CCGC 4 cut(s) 36, 48, 245, 323
SstI GAGCTC 1 cut(s) 365
StuI AGGCCT 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 134
StyI CCWWGG 1 cut(s) 18
TaqI TCGA 3 cut(s) 42, 66, 386
TasI AATT 2 cut(s) 181, 403
TauI GCSGC 1 cut(s) 247
TfiI GAWTC 2 cut(s) 202, 383
TscAI CASTG 2 cut(s) 35, 285
TseFI GTSAC 2 cut(s) 307, 458
TseI GCWGC 2 cut(s) 320, 346
Tsp45I GTSAC 2 cut(s) 307, 458
TspDTI ATGAA 2 cut(s) 97, 330
TspRI CASTG 2 cut(s) 35, 285
XapI RAATTY 1 cut(s) 181
XceI RCATGY 1 cut(s) 346
XmiI GTMKAC 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.