AT4G34320

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010428
Physical Location & Seq
Forward (+)
16420416 .. 16423986
3571 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G34320.2

Sequence Viewer

Length: 1125 bp
ATGGGAAACCAAACAAGCAAAAAGTCACAAGAGACATCGGCTAAGAGCGTGCACTACACAACGGAGCTGAGATCCTACGCGGCTGCGTGCAAAGCAGACACGGAGCTTCAATCTTTCGACACGTGTTTGCAAGCACGGACGAGTCACGTGATAAGCACGCTAGCTACGGGGGTTGAGGTTCGAGCGCTCTCGTTCGATTCCTTGAAAGAAGTGACGCAATGCTTGTTGGAGATGAACCAAGAAGTGGTGAAAGTGATATTGGATTGCAAGAAAGACATTTGGAAGAATCAAGAAATGTTTGAGCTTGTCGAAGATTACTTTGAGAATAGCTTGAAGACACTTGATTTTTGTGCTGCTTTGGAGAAAGGCTTGAGACGAGCTCGTGATAGTCATCTTTTGATTCTTGTTGCTCTTCAACAGTTTGAAGATGAGAGCCTTGTCCAAGGTGGGAACGGATACAAGAAGACACTTGAAGAGCTAAAGAATTTCAAAGACGCAGAGAGTCCTTTCAACGAAGACTTCTTCAAAATGTTCCAATCTGTTTACAAACAGCAGATGCTGATGCTCGAGAAGCTTCAGCATCGTAAAAACAAGCTTGACAAGAAGCTTAAATGCATCCACACATGGAGAAAACTCTCCAGCATCATCTTTGTGGCTACCTTTGCCACTGTGCTCATCTGCTCTGTTGTGGCTGCAGCCATGGCAGCTCCTCCTGTGGCTGCGGCTCTCGCTGCAGCTACAGCCGTGCCGCTAGGCTCAATGGGGAAATGGATTGACTCGCTGTGGAAGAATTATGAGAATGCACTCAAAGGGCAAAAAGAGGTGATCAGTTCGATGCAGGCAGGAACATTTGTGGCGGTTAAAGATTTGGATAATATCCGAGTTTTGATCGAACGGTTGGAGATTGAGATCACGGGGATGGTGAAGAGTGCGGAGTTCGCTGTGGAACATAACGCGGTCAAGATTGGGATCGATGATATAAAGAAGAAGCTTGAGGTGTTTAAGAAGAATGTTGAAGAACTGGGGACTCAAGCAGATTTGTGTAGCAGAGATATAAGAAGGGCAAGGACTGTGATTCTTCAGAGGATCATCAAGCACCCTAATAATGCTAGTAGTAGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

374

Amino Acids

42.05

Weight (kDa)

8.47

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 30 - 158 2.2e-09 Protein ROH1-like
DUF677 PF05055 36 - 363 1.1e-147 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 244
AccII CGCG 2 cut(s) 80, 956
AciI CCGC 6 cut(s) 80, 722, 749, 857, 932, 956
AclWI GGATC 3 cut(s) 66, 977, 1094
AcsI RAATTY 1 cut(s) 484
AcuI CTGAAG 2 cut(s) 560, 1064
AcvI CACGTG 2 cut(s) 123, 148
AfeI AGCGCT 1 cut(s) 186
AfiI CCNNNNNNNGG 1 cut(s) 244
AflIII ACRYGT 2 cut(s) 120, 122
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
Alw21I GWGCWC 3 cut(s) 54, 382, 675
Alw26I GTCTC 2 cut(s) 26, 367
Alw44I GTGCAC 1 cut(s) 50
AlwI GGATC 3 cut(s) 66, 977, 1094
AlwNI CAGNNNCTG 1 cut(s) 559
Ama87I CYCGRG 1 cut(s) 566
Aor51HI AGCGCT 1 cut(s) 186
ApaLI GTGCAC 1 cut(s) 50
ApeKI GCWGC 8 cut(s) 83, 353, 692, 695, 704, 719, 731, 734
ApoI RAATTY 1 cut(s) 484
AspLEI GCGC 1 cut(s) 187
AsuHPI GGTGA 3 cut(s) 259, 835, 934
AsuNHI GCTAGC 1 cut(s) 160
AvaI CYCGRG 1 cut(s) 566
BaeGI GKGCMC 1 cut(s) 54
BanII GRGCYC 1 cut(s) 382
BauI CACGAG 1 cut(s) 381
BbrPI CACGTG 2 cut(s) 123, 148
BbsI GAAGAC 3 cut(s) 341, 470, 522
Bbv12I GWGCWC 3 cut(s) 54, 382, 675
BbvI GCAGC 8 cut(s) 70, 340, 679, 706, 707, 716, 718, 746
BccI CCATC 1 cut(s) 913
BceAI ACGGC 1 cut(s) 728
BciVI GTATCC 1 cut(s) 449
BclI TGATCA 1 cut(s) 825
BcoDI GTCTC 2 cut(s) 26, 367
BfaI CTAG 3 cut(s) 161, 752, 1110
BfmI CTRYAG 3 cut(s) 693, 732, 738
BfoI RGCGCY 1 cut(s) 188
BfuI GTATCC 1 cut(s) 449
BmeT110I CYCGRG 1 cut(s) 566
BmrI ACTGGG 1 cut(s) 1031
BmsI GCATC 6 cut(s) 546, 552, 589, 624, 651, 825
BmtI GCTAGC 1 cut(s) 164
BmuI ACTGGG 1 cut(s) 1031
BpiI GAAGAC 3 cut(s) 341, 470, 522
BplI GAGNNNNNCTC 2 cut(s) 364, 396
BpmI CTGGAG 1 cut(s) 622
BpuEI CTTGAG 3 cut(s) 391, 1013, 1014
Bsa29I ATCGAT 1 cut(s) 972
BsaAI YACGTR 2 cut(s) 123, 148
BsaBI GATNNNNATC 3 cut(s) 390, 908, 968
BsaJI CCNNGG 2 cut(s) 442, 699
Bsc4I CCNNNNNNNGG 1 cut(s) 244
Bse1I ACTGG 1 cut(s) 1026
Bse3DI GCAATG 1 cut(s) 224
Bse8I GATNNNNATC 3 cut(s) 390, 908, 968
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 2 cut(s) 442, 699
BseGI GGATG 2 cut(s) 615, 924
BseJI GATNNNNATC 3 cut(s) 390, 908, 968
BseLI CCNNNNNNNGG 1 cut(s) 244
BseMI GCAATG 1 cut(s) 224
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 1 cut(s) 1026
BseRI GAGGAG 1 cut(s) 699
BseSI GKGCMC 1 cut(s) 54
BseXI GCAGC 8 cut(s) 70, 340, 679, 706, 707, 716, 718, 746
Bsh1236I CGCG 2 cut(s) 80, 956
BshVI ATCGAT 1 cut(s) 972
BsiHKAI GWGCWC 3 cut(s) 54, 382, 675
BsiHKCI CYCGRG 1 cut(s) 566
BslFI GGGAC 1 cut(s) 1039
BslI CCNNNNNNNGG 1 cut(s) 244
BsmAI GTCTC 2 cut(s) 26, 367
BsmBI CGTCTC 1 cut(s) 367
BsmFI GGGAC 1 cut(s) 1039
BsmI GAATGC 1 cut(s) 805
BsoBI CYCGRG 1 cut(s) 566
Bsp1286I GDGCHC 3 cut(s) 54, 382, 675
Bsp143I GATC 6 cut(s) 71, 825, 888, 909, 969, 1086
Bsp19I CCATGG 1 cut(s) 699
BspACI CCGC 6 cut(s) 80, 722, 749, 857, 932, 956
BspCNI CTCAG 1 cut(s) 60
BspDI ATCGAT 1 cut(s) 972
BspFNI CGCG 2 cut(s) 80, 956
BspMAI CTGCAG 2 cut(s) 697, 736
BspOI GCTAGC 1 cut(s) 164
BspPI GGATC 3 cut(s) 66, 977, 1094
BspQI GCTCTTC 2 cut(s) 417, 468
BsrDI GCAATG 1 cut(s) 224
BsrI ACTGG 1 cut(s) 1026
BssECI CCNNGG 2 cut(s) 442, 699
BssMI GATC 6 cut(s) 71, 825, 888, 909, 969, 1086
BssSI CACGAG 1 cut(s) 381
BssT1I CCWWGG 2 cut(s) 442, 699
Bst2BI CACGAG 1 cut(s) 381
Bst4CI ACNGT 4 cut(s) 420, 670, 897, 1072
Bst6I CTCTTC 3 cut(s) 417, 468, 920
BstBAI YACGTR 2 cut(s) 123, 148
BstC8I GCNNGC 6 cut(s) 50, 88, 132, 158, 162, 840
BstDEI CTNAG 2 cut(s) 42, 68
BstDSI CCRYGG 1 cut(s) 699
BstF5I GGATG 2 cut(s) 615, 924
BstFNI CGCG 2 cut(s) 80, 956
BstH2I RGCGCY 1 cut(s) 188
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 6 cut(s) 74, 828, 891, 912, 972, 1089
BstMAI GTCTC 2 cut(s) 26, 367
BstMBI GATC 6 cut(s) 71, 825, 888, 909, 969, 1086
BstMWI GCNNNNNNNGC 9 cut(s) 92, 571, 662, 701, 704, 728, 731, 740, 938
BstSFI CTRYAG 3 cut(s) 693, 732, 738
BstSLI GKGCMC 1 cut(s) 54
BstUI CGCG 2 cut(s) 80, 956
BstV1I GCAGC 8 cut(s) 70, 340, 679, 706, 707, 716, 718, 746
BstV2I GAAGAC 3 cut(s) 341, 470, 522
BstX2I RGATCY 1 cut(s) 71
BstYI RGATCY 1 cut(s) 71
Bsu15I ATCGAT 1 cut(s) 972
BsuI GTATCC 1 cut(s) 449
BsuTUI ATCGAT 1 cut(s) 972
BtgI CCRYGG 1 cut(s) 699
BtsCI GGATG 2 cut(s) 615, 924
BtsIMutI CAGTG 1 cut(s) 666
Cac8I GCNNGC 6 cut(s) 50, 88, 132, 158, 162, 840
CaiI CAGNNNCTG 1 cut(s) 559
CfoI GCGC 1 cut(s) 187
ClaI ATCGAT 1 cut(s) 972
CseI GACGC 2 cut(s) 223, 503
CviAII CATG 2 cut(s) 624, 700
DdeI CTNAG 2 cut(s) 42, 68
DpnI GATC 6 cut(s) 73, 827, 890, 911, 971, 1088
DpnII GATC 6 cut(s) 71, 825, 888, 909, 969, 1086
Eam1104I CTCTTC 3 cut(s) 417, 468, 920
EarI CTCTTC 3 cut(s) 417, 468, 920
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 2 cut(s) 442, 699
Eco24I GRGCYC 1 cut(s) 382
Eco47III AGCGCT 1 cut(s) 186
Eco53kI GAGCTC 1 cut(s) 380
Eco57I CTGAAG 2 cut(s) 560, 1064
Eco72I CACGTG 2 cut(s) 123, 148
Eco88I CYCGRG 1 cut(s) 566
EcoICRI GAGCTC 1 cut(s) 380
EcoT14I CCWWGG 2 cut(s) 442, 699
EcoT22I ATGCAT 1 cut(s) 617
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 2 cut(s) 442, 699
Esp3I CGTCTC 1 cut(s) 367
FaeI CATG 2 cut(s) 627, 703
FaiI YATR 6 cut(s) 625, 701, 795, 951, 980, 1055
FaqI GGGAC 1 cut(s) 1039
FatI CATG 2 cut(s) 623, 699
FbaI TGATCA 1 cut(s) 825
FokI GGATG 2 cut(s) 602, 931
FriOI GRGCYC 1 cut(s) 382
FspBI CTAG 3 cut(s) 161, 752, 1110
GlaI GCGC 1 cut(s) 186
GsuI CTGGAG 1 cut(s) 622
HaeII RGCGCY 1 cut(s) 188
HgaI GACGC 2 cut(s) 223, 503
HhaI GCGC 1 cut(s) 187
Hin1II CATG 2 cut(s) 627, 703
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HindIII AAGCTT 4 cut(s) 572, 593, 605, 989
HinfI GANTC 8 cut(s) 142, 197, 286, 400, 502, 776, 1027, 1075
HphI GGTGA 3 cut(s) 259, 835, 934
Hpy166II GTNNAC 2 cut(s) 52, 544
Hpy188I TCNGA 2 cut(s) 881, 1083
Hpy188III TCNNGA 4 cut(s) 290, 383, 568, 961
Hpy8I GTNNAC 2 cut(s) 52, 544
HpyAV CCTTC 1 cut(s) 1053
HpyCH4III ACNGT 4 cut(s) 420, 670, 897, 1072
HpyCH4IV ACGT 2 cut(s) 122, 147
HpyCH4V TGCA 9 cut(s) 52, 90, 130, 267, 615, 695, 734, 803, 838
HpyF10VI GCNNNNNNNGC 9 cut(s) 92, 571, 662, 701, 704, 728, 731, 740, 938
HpyF3I CTNAG 2 cut(s) 42, 68
HpySE526I ACGT 2 cut(s) 122, 147
Hsp92II CATG 2 cut(s) 627, 703
HspAI GCGC 1 cut(s) 185
Ksp22I TGATCA 1 cut(s) 825
Kzo9I GATC 6 cut(s) 71, 825, 888, 909, 969, 1086
LguI GCTCTTC 2 cut(s) 417, 468
LmnI GCTCC 3 cut(s) 64, 103, 712
LpnPI CCDG 5 cut(s) 652, 726, 824, 828, 1007
Lsp1109I GCAGC 8 cut(s) 70, 340, 679, 706, 707, 716, 718, 746
LweI GCATC 6 cut(s) 546, 552, 589, 624, 651, 825
MaeI CTAG 3 cut(s) 161, 752, 1110
MaeII ACGT 2 cut(s) 122, 147
MaeIII GTNAC 3 cut(s) 24, 143, 211
MalI GATC 6 cut(s) 73, 827, 890, 911, 971, 1088
MboI GATC 6 cut(s) 71, 825, 888, 909, 969, 1086
MflI RGATCY 1 cut(s) 71
MhlI GDGCHC 3 cut(s) 54, 382, 675
MluCI AATT 2 cut(s) 484, 790
MlyI GAGTC 4 cut(s) 151, 511, 770, 1021
MmeI TCCRAC 2 cut(s) 207, 879
MnlI CCTC 5 cut(s) 169, 720, 814, 988, 1077
Mph1103I ATGCAT 1 cut(s) 617
MseI TTAA 3 cut(s) 609, 861, 1002
MslI CAYNNNNRTG 2 cut(s) 650, 917
Mva1269I GAATGC 1 cut(s) 805
MvnI CGCG 2 cut(s) 80, 956
MwoI GCNNNNNNNGC 9 cut(s) 92, 571, 662, 701, 704, 728, 731, 740, 938
NcoI CCATGG 1 cut(s) 699
NdeII GATC 6 cut(s) 71, 825, 888, 909, 969, 1086
NheI GCTAGC 1 cut(s) 160
NlaIII CATG 2 cut(s) 627, 703
NmuCI GTSAC 3 cut(s) 24, 143, 211
NsiI ATGCAT 1 cut(s) 617
PaeR7I CTCGAG 1 cut(s) 566
PciSI GCTCTTC 2 cut(s) 417, 468
PctI GAATGC 1 cut(s) 805
PfeI GAWTC 4 cut(s) 197, 286, 400, 1075
PflMI CCANNNNNTGG 1 cut(s) 244
PleI GAGTC 4 cut(s) 150, 510, 770, 1021
PmaCI CACGTG 2 cut(s) 123, 148
PmlI CACGTG 2 cut(s) 123, 148
PpsI GAGTC 4 cut(s) 150, 510, 770, 1021
Ppu21I YACGTR 2 cut(s) 123, 148
Psp124BI GAGCTC 1 cut(s) 382
PspCI CACGTG 2 cut(s) 123, 148
PstI CTGCAG 2 cut(s) 697, 736
PstNI CAGNNNCTG 1 cut(s) 559
PsuI RGATCY 1 cut(s) 71
RseI CAYNNNNRTG 2 cut(s) 650, 917
SacI GAGCTC 1 cut(s) 382
SapI GCTCTTC 2 cut(s) 417, 468
SaqAI TTAA 3 cut(s) 609, 861, 1002
Sau3AI GATC 6 cut(s) 71, 825, 888, 909, 969, 1086
SchI GAGTC 4 cut(s) 151, 511, 770, 1021
SduI GDGCHC 3 cut(s) 54, 382, 675
SfaNI GCATC 6 cut(s) 546, 552, 589, 624, 651, 825
SfcI CTRYAG 3 cut(s) 693, 732, 738
Sfr274I CTCGAG 1 cut(s) 566
SlaI CTCGAG 1 cut(s) 566
SmiMI CAYNNNNRTG 2 cut(s) 650, 917
SmlI CTYRAG 4 cut(s) 370, 566, 992, 1029
SmoI CTYRAG 4 cut(s) 370, 566, 992, 1029
Sse9I AATT 2 cut(s) 484, 790
SsiI CCGC 6 cut(s) 80, 722, 749, 857, 932, 956
SspMI CTAG 3 cut(s) 161, 752, 1110
SstI GAGCTC 1 cut(s) 382
StyI CCWWGG 2 cut(s) 442, 699
TaaI ACNGT 4 cut(s) 420, 670, 897, 1072
TaiI ACGT 2 cut(s) 125, 150
TaqI TCGA 8 cut(s) 117, 181, 195, 309, 567, 833, 891, 972
TasI AATT 2 cut(s) 484, 790
TauI GCSGC 3 cut(s) 83, 725, 751
TfiI GAWTC 4 cut(s) 197, 286, 400, 1075
Tru1I TTAA 3 cut(s) 609, 861, 1002
Tru9I TTAA 3 cut(s) 609, 861, 1002
TscAI CASTG 1 cut(s) 673
TseFI GTSAC 3 cut(s) 24, 143, 211
TseI GCWGC 8 cut(s) 83, 353, 692, 695, 704, 719, 731, 734
Tsp45I GTSAC 3 cut(s) 24, 143, 211
TspDTI ATGAA 1 cut(s) 248
TspGWI ACGGA 4 cut(s) 77, 116, 151, 468
TspRI CASTG 1 cut(s) 673
Van91I CCANNNNNTGG 1 cut(s) 244
VneI GTGCAC 1 cut(s) 50
XapI RAATTY 1 cut(s) 484
XhoI CTCGAG 1 cut(s) 566
XspI CTAG 3 cut(s) 161, 752, 1110
Zsp2I ATGCAT 1 cut(s) 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.