Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0010428

Physical Location & Seq

Genomic Coordinates Forward (+)

12008957 .. 12010739

Gene Structure 1783 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1081600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1113 bp

ATGAGCAAAAGGAGCGCTGAAACATCGTCATCTGCTATCAATCTGAACAATTTGCAATACACAACTGATCAGTTGACTTCATATGAGGCAGCTTGCAAGCTCGATGCAGACTTGCAGTCCTTCGACACGAACCTCCATACCCGGACCAATCAAGTCATCAACACAATCGCAGCAGGAGTCGAAGTCCGAGCCCTTTCCTTCGATTCCTTGAAGGAGGTGACAGGATGTCTGTTGGAGATGAATCAGGAAGTTGTGAAAGTTATCTTGGAGTGCAAGAAAGACATATGGAAAAATCAGGAGTTGTTTGAGCTGGTTGAGGAGTATTTTGAGAACAGCTTGCAGACACTGGATTTCTGTACTGCATTGGACAAGTGTTTGAAGCGAGCTCGCGATAGCCAGTTGCTTATTCTCGTTGCGCTTCAGCAATTCGAGGAGGAAACCGAAATGGGAGGCAGTCGATACACGAGGACTTTGGAGGAATTGAAGAATTTCAAGACGATAGGTGATCCTTTTACTGAGGAGTTCTTTCAAATATTTCAATCTGTTTATAAGCAACAAATATCAATGCTCGAGAAGTTGCAACTGCGAAAGAACAAGCTCGATAAGAAGCTCAAAGGTATTAATGCTTGGAGGAAGGTGACTAATATGATATTTGTTGCTACATTCGCTGCCGTGTTGATTTGTTCTGTTGTGGCGGCAGCCATGGCTGCTCCACCCGTCGCAGCAGCTCTGGCCGCTGCTAGTTCTATCCCGATAGGCTCAATGGGGAAGTGGATTGACTCCTTGTGGAAAAACTACGAACTTGCTTTGAGGGGTCAAAGGGAAGTGCTTAGCTCAATGCAAGTAGGAACTTATGTTGCCATTAAGGACTTGGACAACATTCGGGTTCTCATTGATCGATTGGAAATTGATATCGAGTCCATCTTGCACAATGCAAGCTTTGCCATTGAGGAAGACGCGGTGAAAGTTGCAATAGAGGACATTAAAAAGAAGTTGGGAGTGTTTATGAAGAATGTGGAGGATTTGGGAACTCAAGCTGATATCTGCAGCCGCGACATTCGAAGAGCAAGGACCGTGGTTCTCCAGAGGATCATCAAACATTCCAACAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF677

Protein Analysis

371

Amino Acids

41.77

Weight (kDa)

5.69

Isoelectric Point (pI)

44.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ROH1-like	PF05633	15 - 156	7e-08	Protein ROH1-like
DUF677	PF05055	38 - 364	1.1e-136	Protein of unknown function (DUF677)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010428)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34320 AT4G34320
fragaria_vesca	FvH4_2g08180 FvH4_2g08180
malus_domestica	MD05G1070600.v1.1 MD10G1081600.v1.1
prunus_persica	Prupe.8G103100_v2.0.a1 Prupe.8G103100_v2.0.a1 Prupe.8G103100_v2.0.a1
pyrus_communis	pycom10g06450
rosa_chinensis	RchiOBHm_Chr6g0262961
rosa_laevigata	RLG00000014389
rosa_multiflora	Rmu_sc0006493.1_g000001
rosa_roxburghii	Rroxscaffold_7G00204820
rosa_rugosa	Rorug06G0002500 Rorug06G0002600
rosa_samantha	Rh6AG121300 Rh6BG114900 Rh6CG113300 Rh6DG102600
rosa_wichuraiana	Rw6G010410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	549
AccII	CGCG	3 cut(s)	390, 959, 1053
AciI	CCGC	4 cut(s)	695, 735, 959, 1051
AclWI	GGATC	2 cut(s)	500, 1097
AcoI	YGGCCR	1 cut(s)	732
AcsI	RAATTY	1 cut(s)	487
AcuI	CTGAAG	1 cut(s)	404
AfaI	GTAC	1 cut(s)	358
AfeI	AGCGCT	1 cut(s)	16
AgsI	TTSAA	6 cut(s)	211, 379, 484, 493, 530, 539
AhdI	GACNNNNNGTC	2 cut(s)	115, 225
AjuI	GAANNNNNNNTTGG	2 cut(s)	248, 280
Alw21I	GWGCWC	1 cut(s)	388
AlwI	GGATC	2 cut(s)	500, 1097
AlwNI	CAGNNNCTG	1 cut(s)	346
Ama87I	CYCGRG	1 cut(s)	569
Aor51HI	AGCGCT	1 cut(s)	16
AoxI	GGCC	1 cut(s)	732
ApeKI	GCWGC	9 cut(s)	89, 170, 668, 698, 707, 722, 725, 737, 1047
ApoI	RAATTY	1 cut(s)	487
AseI	ATTAAT	1 cut(s)	621
Asp700I	GAANNNNTTC	1 cut(s)	488
AspLEI	GCGC	2 cut(s)	17, 418
AspS9I	GGNCC	2 cut(s)	144, 1071
AsuC2I	CCSGG	1 cut(s)	142
AsuHPI	GGTGA	4 cut(s)	229, 515, 649, 973
AsuII	TTCGAA	1 cut(s)	1060
AvaI	CYCGRG	1 cut(s)	569
AvaII	GGWCC	2 cut(s)	144, 1071
BanII	GRGCYC	2 cut(s)	193, 388
BauI	CACGAG	1 cut(s)	463
BbsI	GAAGAC	1 cut(s)	960
Bbv12I	GWGCWC	1 cut(s)	388
BbvI	GCAGC	9 cut(s)	101, 182, 655, 694, 710, 724, 734, 737, 1059
BccI	CCATC	1 cut(s)	929
BceAI	ACGGC	1 cut(s)	656
BclI	TGATCA	1 cut(s)	67
BcnI	CCSGG	1 cut(s)	142
BfaI	CTAG	1 cut(s)	741
BfmI	CTRYAG	1 cut(s)	1045
BfoI	RGCGCY	1 cut(s)	18
BlpI	GCTNAGC	1 cut(s)	830
Bme1390I	CCNGG	1 cut(s)	142
Bme18I	GGWCC	2 cut(s)	144, 1071
BmeRI	GACNNNNNGTC	2 cut(s)	115, 225
BmeT110I	CYCGRG	1 cut(s)	569
BmgT120I	GGNCC	2 cut(s)	144, 1071
BmrFI	CCNGG	1 cut(s)	142
BmsI	GCATC	1 cut(s)	94
BpiI	GAAGAC	1 cut(s)	960
BpmI	CTGGAG	1 cut(s)	1067
Bpu1102I	GCTNAGC	1 cut(s)	830
Bpu14I	TTCGAA	1 cut(s)	1060
BpuEI	CTTGAG	1 cut(s)	1017
BpuMI	CCSGG	1 cut(s)	142
Bsa29I	ATCGAT	1 cut(s)	898
BsaJI	CCNNGG	2 cut(s)	702, 1074
BsaXI	ACNNNNNCTCC	2 cut(s)	168, 198
Bse1I	ACTGG	2 cut(s)	351, 397
BseCI	ATCGAT	1 cut(s)	898
BseDI	CCNNGG	2 cut(s)	702, 1074
BseGI	GGATG	1 cut(s)	230
BseMII	CTCAG	1 cut(s)	507
BseNI	ACTGG	2 cut(s)	351, 397
BseRI	GAGGAG	3 cut(s)	332, 446, 533
BseXI	GCAGC	9 cut(s)	101, 182, 655, 694, 710, 724, 734, 737, 1059
Bsh1236I	CGCG	3 cut(s)	390, 959, 1053
BshFI	GGCC	1 cut(s)	734
BshVI	ATCGAT	1 cut(s)	898
BsiHKAI	GWGCWC	1 cut(s)	388
BsiHKCI	CYCGRG	1 cut(s)	569
BsiSI	CCGG	1 cut(s)	142
BsnI	GGCC	1 cut(s)	734
BsoBI	CYCGRG	1 cut(s)	569
Bsp119I	TTCGAA	1 cut(s)	1060
Bsp1286I	GDGCHC	2 cut(s)	193, 388
Bsp143I	GATC	4 cut(s)	67, 505, 895, 1089
Bsp1720I	GCTNAGC	1 cut(s)	830
Bsp19I	CCATGG	1 cut(s)	702
Bsp68I	TCGCGA	1 cut(s)	390
BspACI	CCGC	4 cut(s)	695, 735, 959, 1051
BspANI	GGCC	1 cut(s)	734
BspCNI	CTCAG	1 cut(s)	508
BspDI	ATCGAT	1 cut(s)	898
BspFNI	CGCG	3 cut(s)	390, 959, 1053
BspMAI	CTGCAG	1 cut(s)	1049
BspPI	GGATC	2 cut(s)	500, 1097
BspQI	GCTCTTC	1 cut(s)	1057
BspT104I	TTCGAA	1 cut(s)	1060
BsrI	ACTGG	2 cut(s)	351, 397
BssECI	CCNNGG	2 cut(s)	702, 1074
BssMI	GATC	4 cut(s)	67, 505, 895, 1089
BssSI	CACGAG	1 cut(s)	463
BssT1I	CCWWGG	1 cut(s)	702
Bst2BI	CACGAG	1 cut(s)	463
Bst4CI	ACNGT	1 cut(s)	1075
Bst6I	CTCTTC	1 cut(s)	1057
BstAPI	GCANNNNNTGC	1 cut(s)	941
BstBI	TTCGAA	1 cut(s)	1060
BstC8I	GCNNGC	6 cut(s)	94, 98, 338, 384, 388, 937
BstDEI	CTNAG	2 cut(s)	516, 830
BstDSI	CCRYGG	2 cut(s)	702, 1074
BstF5I	GGATG	1 cut(s)	230
BstFNI	CGCG	3 cut(s)	390, 959, 1053
BstH2I	RGCGCY	1 cut(s)	18
BstHHI	GCGC	2 cut(s)	17, 418
BstKTI	GATC	4 cut(s)	70, 508, 898, 1092
BstMBI	GATC	4 cut(s)	67, 505, 895, 1089
BstMWI	GCNNNNNNNGC	7 cut(s)	12, 665, 704, 707, 731, 734, 941
BstSCI	CCNGG	1 cut(s)	140
BstSFI	CTRYAG	1 cut(s)	1045
BstUI	CGCG	3 cut(s)	390, 959, 1053
BstV1I	GCAGC	9 cut(s)	101, 182, 655, 694, 710, 724, 734, 737, 1059
BstV2I	GAAGAC	1 cut(s)	960
Bsu15I	ATCGAT	1 cut(s)	898
BsuRI	GGCC	1 cut(s)	734
BsuTUI	ATCGAT	1 cut(s)	898
BtgI	CCRYGG	2 cut(s)	702, 1074
BtsCI	GGATG	1 cut(s)	230
BtsIMutI	CAGTG	1 cut(s)	344
BtuMI	TCGCGA	1 cut(s)	390
Cac8I	GCNNGC	6 cut(s)	94, 98, 338, 384, 388, 937
CaiI	CAGNNNCTG	1 cut(s)	346
CfoI	GCGC	2 cut(s)	17, 418
Cfr13I	GGNCC	2 cut(s)	144, 1071
ClaI	ATCGAT	1 cut(s)	898
CseI	GACGC	1 cut(s)	965
Csp6I	GTAC	1 cut(s)	357
CspCI	CAANNNNNGTGG	2 cut(s)	1056, 1091
CviAII	CATG	1 cut(s)	703
CviQI	GTAC	1 cut(s)	357
DdeI	CTNAG	2 cut(s)	516, 830
DpnI	GATC	4 cut(s)	69, 507, 897, 1091
DpnII	GATC	4 cut(s)	67, 505, 895, 1089
DriI	GACNNNNNGTC	2 cut(s)	115, 225
EaeI	YGGCCR	1 cut(s)	732
Eam1104I	CTCTTC	1 cut(s)	1057
Eam1105I	GACNNNNNGTC	2 cut(s)	115, 225
EarI	CTCTTC	1 cut(s)	1057
Ecl136II	GAGCTC	1 cut(s)	386
Eco130I	CCWWGG	1 cut(s)	702
Eco24I	GRGCYC	2 cut(s)	193, 388
Eco32I	GATATC	2 cut(s)	913, 1042
Eco47I	GGWCC	2 cut(s)	144, 1071
Eco47III	AGCGCT	1 cut(s)	16
Eco53kI	GAGCTC	1 cut(s)	386
Eco57I	CTGAAG	1 cut(s)	404
Eco88I	CYCGRG	1 cut(s)	569
EcoICRI	GAGCTC	1 cut(s)	386
EcoRV	GATATC	2 cut(s)	913, 1042
EcoT14I	CCWWGG	1 cut(s)	702
EcoT38I	GRGCYC	2 cut(s)	193, 388
ErhI	CCWWGG	1 cut(s)	702
FaeI	CATG	1 cut(s)	706
FatI	CATG	1 cut(s)	702
FauNDI	CATATG	2 cut(s)	82, 284
FbaI	TGATCA	1 cut(s)	67
FokI	GGATG	1 cut(s)	237
FriOI	GRGCYC	2 cut(s)	193, 388
FspBI	CTAG	1 cut(s)	741
GlaI	GCGC	2 cut(s)	16, 417
GsuI	CTGGAG	1 cut(s)	1067
HaeII	RGCGCY	1 cut(s)	18
HaeIII	GGCC	1 cut(s)	734
HapII	CCGG	1 cut(s)	142
HgaI	GACGC	1 cut(s)	965
HhaI	GCGC	2 cut(s)	17, 418
Hin1II	CATG	1 cut(s)	706
Hin6I	GCGC	2 cut(s)	15, 416
HinP1I	GCGC	2 cut(s)	15, 416
HincII	GTYRAC	1 cut(s)	75
HindII	GTYRAC	1 cut(s)	75
HindIII	AAGCTT	1 cut(s)	937
HinfI	GANTC	5 cut(s)	177, 203, 241, 779, 917
HpaII	CCGG	1 cut(s)	142
HphI	GGTGA	4 cut(s)	229, 515, 649, 973
Hpy166II	GTNNAC	1 cut(s)	75
Hpy188I	TCNGA	2 cut(s)	45, 188
Hpy188III	TCNNGA	7 cut(s)	245, 296, 389, 493, 571, 751, 1084
Hpy8I	GTNNAC	1 cut(s)	75
Hpy99I	CGWCG	1 cut(s)	722
HpyAV	CCTTC	4 cut(s)	130, 205, 208, 628
HpyCH4III	ACNGT	1 cut(s)	1075
HpyF10VI	GCNNNNNNNGC	7 cut(s)	12, 665, 704, 707, 731, 734, 941
HpyF3I	CTNAG	2 cut(s)	516, 830
Hsp92II	CATG	1 cut(s)	706
HspAI	GCGC	2 cut(s)	15, 416
Ksp22I	TGATCA	1 cut(s)	67
Kzo9I	GATC	4 cut(s)	67, 505, 895, 1089
LguI	GCTCTTC	1 cut(s)	1057
LmnI	GCTCC	2 cut(s)	12, 715
Lsp1109I	GCAGC	9 cut(s)	101, 182, 655, 694, 710, 724, 734, 737, 1059
LweI	GCATC	1 cut(s)	94
MaeI	CTAG	1 cut(s)	741
MaeIII	GTNAC	2 cut(s)	217, 637
MalI	GATC	4 cut(s)	69, 507, 897, 1091
MboI	GATC	4 cut(s)	67, 505, 895, 1089
MboII	GAAGA	4 cut(s)	496, 965, 1021, 1074
MhlI	GDGCHC	2 cut(s)	193, 388
MluCI	AATT	5 cut(s)	49, 425, 479, 487, 906
MlyI	GAGTC	3 cut(s)	186, 773, 926
MmeI	TCCRAC	1 cut(s)	213
MroXI	GAANNNNTTC	1 cut(s)	488
MseI	TTAA	3 cut(s)	621, 864, 984
MspA1I	CMGCKG	1 cut(s)	737
MspI	CCGG	1 cut(s)	142
MspR9I	CCNGG	1 cut(s)	142
MvnI	CGCG	3 cut(s)	390, 959, 1053
MwoI	GCNNNNNNNGC	7 cut(s)	12, 665, 704, 707, 731, 734, 941
NciI	CCSGG	1 cut(s)	142
NcoI	CCATGG	1 cut(s)	702
NdeI	CATATG	2 cut(s)	82, 284
NdeII	GATC	4 cut(s)	67, 505, 895, 1089
NlaIII	CATG	1 cut(s)	706
NmuCI	GTSAC	2 cut(s)	217, 637
NruI	TCGCGA	1 cut(s)	390
NspV	TTCGAA	1 cut(s)	1060
PaeR7I	CTCGAG	1 cut(s)	569
PciSI	GCTCTTC	1 cut(s)	1057
PdmI	GAANNNNTTC	1 cut(s)	488
PfeI	GAWTC	2 cut(s)	203, 241
PleI	GAGTC	3 cut(s)	185, 773, 925
PpsI	GAGTC	3 cut(s)	185, 773, 925
PshBI	ATTAAT	1 cut(s)	621
PsiI	TTATAA	1 cut(s)	549
Psp124BI	GAGCTC	1 cut(s)	388
PspPI	GGNCC	2 cut(s)	144, 1071
PstI	CTGCAG	1 cut(s)	1049
PstNI	CAGNNNCTG	1 cut(s)	346
RruI	TCGCGA	1 cut(s)	390
RsaI	GTAC	1 cut(s)	358
RsaNI	GTAC	1 cut(s)	357
SacI	GAGCTC	1 cut(s)	388
SapI	GCTCTTC	1 cut(s)	1057
SaqAI	TTAA	3 cut(s)	621, 864, 984
Sau3AI	GATC	4 cut(s)	67, 505, 895, 1089
Sau96I	GGNCC	2 cut(s)	144, 1071
SchI	GAGTC	3 cut(s)	186, 773, 926
ScrFI	CCNGG	1 cut(s)	142
SduI	GDGCHC	2 cut(s)	193, 388
SfaNI	GCATC	1 cut(s)	94
SfcI	CTRYAG	1 cut(s)	1045
Sfr274I	CTCGAG	1 cut(s)	569
SfuI	TTCGAA	1 cut(s)	1060
SinI	GGWCC	2 cut(s)	144, 1071
SlaI	CTCGAG	1 cut(s)	569
SmlI	CTYRAG	2 cut(s)	569, 1032
SmoI	CTYRAG	2 cut(s)	569, 1032
Sse9I	AATT	5 cut(s)	49, 425, 479, 487, 906
SsiI	CCGC	4 cut(s)	695, 735, 959, 1051
SspI	AATATT	1 cut(s)	534
SspMI	CTAG	1 cut(s)	741
SstI	GAGCTC	1 cut(s)	388
StyD4I	CCNGG	1 cut(s)	140
StyI	CCWWGG	1 cut(s)	702
TaaI	ACNGT	1 cut(s)	1075
TasI	AATT	5 cut(s)	49, 425, 479, 487, 906
TatI	WGTACW	1 cut(s)	356
TauI	GCSGC	3 cut(s)	698, 737, 1053
TfiI	GAWTC	2 cut(s)	203, 241
Tru1I	TTAA	3 cut(s)	621, 864, 984
Tru9I	TTAA	3 cut(s)	621, 864, 984
TscAI	CASTG	1 cut(s)	351
TseFI	GTSAC	2 cut(s)	217, 637
TseI	GCWGC	9 cut(s)	89, 170, 668, 698, 707, 722, 725, 737, 1047
Tsp45I	GTSAC	2 cut(s)	217, 637
TspDTI	ATGAA	3 cut(s)	69, 254, 1022
TspRI	CASTG	1 cut(s)	351
VpaK11BI	GGWCC	2 cut(s)	144, 1071
VspI	ATTAAT	1 cut(s)	621
XapI	RAATTY	1 cut(s)	487
XcmI	CCANNNNNNNNNTGG	1 cut(s)	868
XhoI	CTCGAG	1 cut(s)	569
XmnI	GAANNNNTTC	1 cut(s)	488
XspI	CTAG	1 cut(s)	741

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.