AT4G34560

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014184
Physical Location & Seq
Forward (+)
16507616 .. 16508824
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G34560.1

Sequence Viewer

Length: 666 bp
ATGGATCCAATTAAGTTTCAACACTTGAAGATGGAGAAATCCAGAGGAATCTTGAATTTCCAAGCAATAAGATCGTTCACGAGCTTGTTCAGACTGTTCGAGCTTCTTCTTCTTGTAATCTTGATCTCCAAACTCTCTTTCCCTTCTGTAAAAATCTCTGGGGACATTTTCAAGGAAGCAGCAGTGTTTCTCGTTAGCCCGAGATTCGTTTTCTTCATCGGAAACGCAATTGTAATTACTCTTCTTGCGAAATCCGGACGATACAGCTCGAGTCACGAGCCTTCAAAGACAGCAACAGAAGCTGCAAGCAACGATCTTTACCAAGAATTTCTTCACAAGAGTGAGAAGAAGAAACCCGTAGTTTACGCTACGAAAACAGAACAACTAAAGAAGCCGAGTGGAGGGAAGAGGGTTAGTTTCGGGAGAAGCCAATCGCACAAAGCGTTTGAGGCGGTTAAGCCGCTGGAGAGTACCAATGGTGAGAAAAACATGAAGAGGTATGTGTCGGAGAAGCATATGAGGGTTTGTGACTCCGACAAGAAAGTAGTGGTGAGAGTTAAGAAACCAGAAGATGGAATGAGCAACGAGCAATTTAGGACAAAGATTGAAGCTTTCATCGCTAGACAAAAGAGAATTCAAAAGGATGAAGAACACTTGATAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408

Protein Analysis

221

Amino Acids

25.28

Weight (kDa)

9.95

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34560
fragaria_vesca FvH4_2g09200
malus_domestica MD05G1064000.v1.1 MD10G1072700.v1.1
prunus_persica Prupe.6G200500_v2.0.a1 Prupe.8G094100_v2.0.a1
pyrus_communis pycom10g05740
rosa_chinensis RchiOBHm_Chr6g0265051
rosa_laevigata RLG00000014213
rosa_roxburghii Rroxscaffold_175G00432120 Rroxscaffold_7G00203000
rosa_rugosa Rorug06G0013400
rosa_samantha Rh6AG136400 Rh6BG133400 Rh6CG131800 Rh6DG119100
rosa_wichuraiana Rw6G011770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 572
AccIII TCCGGA 1 cut(s) 254
AciI CCGC 2 cut(s) 452, 461
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 3 cut(s) 55, 326, 633
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 2 cut(s) 401, 572
AgsI TTSAA 7 cut(s) 20, 28, 55, 172, 285, 608, 638
AjuI GAANNNNNNNTTGG 4 cut(s) 122, 154, 315, 347
AleI CACNNNNGTG 1 cut(s) 339
AluBI AGCT 5 cut(s) 84, 103, 267, 302, 611
AluI AGCT 5 cut(s) 84, 103, 267, 302, 611
AlwI GGATC 1 cut(s) 12
AlwNI CAGNNNCTG 1 cut(s) 302
Ama87I CYCGRG 2 cut(s) 199, 268
Aor13HI TCCGGA 1 cut(s) 254
ApeKI GCWGC 2 cut(s) 179, 302
ApoI RAATTY 3 cut(s) 55, 326, 633
Asp700I GAANNNNTTC 1 cut(s) 330
AsuHPI GGTGA 2 cut(s) 491, 562
AvaI CYCGRG 2 cut(s) 199, 268
BamHI GGATCC 1 cut(s) 4
BarI GAAGNNNNNNTAC 2 cut(s) 225, 257
BauI CACGAG 2 cut(s) 79, 275
BbvI GCAGC 2 cut(s) 191, 289
BccI CCATC 2 cut(s) 25, 566
BcgI CGANNNNNNTGC 2 cut(s) 54, 88
BfaI CTAG 2 cut(s) 621, 664
BisI GCNGC 3 cut(s) 180, 303, 461
BlsI GCNGC 3 cut(s) 181, 304, 462
BmeT110I CYCGRG 2 cut(s) 199, 268
BmiI GGNNCC 1 cut(s) 6
BpmI CTGGAG 1 cut(s) 485
BsaWI WCCGGW 1 cut(s) 254
Bsc4I CCNNNNNNNGG 2 cut(s) 401, 572
BseAI TCCGGA 1 cut(s) 254
BseGI GGATG 1 cut(s) 649
BseLI CCNNNNNNNGG 2 cut(s) 401, 572
BseXI GCAGC 2 cut(s) 191, 289
BsiHKCI CYCGRG 2 cut(s) 199, 268
BsiSI CCGG 1 cut(s) 255
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 2 cut(s) 401, 572
BsmFI GGGAC 1 cut(s) 176
BsoBI CYCGRG 2 cut(s) 199, 268
Bsp13I TCCGGA 1 cut(s) 254
Bsp143I GATC 4 cut(s) 4, 71, 123, 313
BspACI CCGC 2 cut(s) 452, 461
BspEI TCCGGA 1 cut(s) 254
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 12
BssMI GATC 4 cut(s) 4, 71, 123, 313
BssSI CACGAG 2 cut(s) 79, 275
Bst2BI CACGAG 2 cut(s) 79, 275
Bst4CI ACNGT 1 cut(s) 96
Bst6I CTCTTC 3 cut(s) 246, 401, 488
BstC8I GCNNGC 1 cut(s) 307
BstF5I GGATG 1 cut(s) 649
BstKTI GATC 4 cut(s) 7, 74, 126, 316
BstMBI GATC 4 cut(s) 4, 71, 123, 313
BstMWI GCNNNNNNNGC 3 cut(s) 299, 449, 617
BstV1I GCAGC 2 cut(s) 191, 289
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BtgZI GCGATG 1 cut(s) 601
BtsCI GGATG 1 cut(s) 649
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 1 cut(s) 307
CaiI CAGNNNCTG 1 cut(s) 302
Csp6I GTAC 1 cut(s) 471
CviAII CATG 1 cut(s) 490
CviQI GTAC 1 cut(s) 471
DpnI GATC 4 cut(s) 6, 73, 125, 315
DpnII GATC 4 cut(s) 4, 71, 123, 313
Eam1104I CTCTTC 3 cut(s) 246, 401, 488
EarI CTCTTC 3 cut(s) 246, 401, 488
Eco88I CYCGRG 2 cut(s) 199, 268
EcoRI GAATTC 1 cut(s) 633
FaeI CATG 1 cut(s) 493
FaiI YATR 4 cut(s) 491, 501, 516, 518
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FaqI GGGAC 1 cut(s) 176
FatI CATG 1 cut(s) 489
FauNDI CATATG 1 cut(s) 516
Fnu4HI GCNGC 3 cut(s) 180, 303, 461
FokI GGATG 1 cut(s) 656
Fsp4HI GCNGC 3 cut(s) 180, 303, 461
FspBI CTAG 2 cut(s) 621, 664
GluI GCNGC 3 cut(s) 180, 303, 461
GsuI CTGGAG 1 cut(s) 485
HapII CCGG 1 cut(s) 255
Hin1II CATG 1 cut(s) 493
HindIII AAGCTT 1 cut(s) 609
HinfI GANTC 4 cut(s) 48, 204, 271, 530
HpaII CCGG 1 cut(s) 255
HphI GGTGA 2 cut(s) 491, 562
Hpy166II GTNNAC 2 cut(s) 78, 364
Hpy188I TCNGA 4 cut(s) 92, 221, 508, 535
Hpy188III TCNNGA 7 cut(s) 42, 52, 79, 121, 255, 275, 421
Hpy8I GTNNAC 2 cut(s) 78, 364
HpyAV CCTTC 2 cut(s) 153, 291
HpyCH4III ACNGT 1 cut(s) 96
HpyCH4V TGCA 1 cut(s) 305
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 449, 617
Hsp92II CATG 1 cut(s) 493
Kpn2I TCCGGA 1 cut(s) 254
Kzo9I GATC 4 cut(s) 4, 71, 123, 313
LpnPI CCDG 5 cut(s) 55, 144, 268, 449, 579
Lsp1109I GCAGC 2 cut(s) 191, 289
MaeI CTAG 2 cut(s) 621, 664
MaeIII GTNAC 2 cut(s) 272, 527
MalI GATC 4 cut(s) 6, 73, 125, 315
MboI GATC 4 cut(s) 4, 71, 123, 313
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 1 cut(s) 4
MluCI AATT 7 cut(s) 9, 55, 228, 234, 326, 590, 633
MlyI GAGTC 2 cut(s) 280, 524
MmeI TCCRAC 2 cut(s) 486, 558
MnlI CCTC 6 cut(s) 38, 395, 402, 442, 489, 513
MroI TCCGGA 1 cut(s) 254
MroXI GAANNNNTTC 1 cut(s) 330
MseI TTAA 3 cut(s) 12, 456, 558
MslI CAYNNNNRTG 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 463
MspI CCGG 1 cut(s) 255
MunI CAATTG 1 cut(s) 228
MwoI GCNNNNNNNGC 3 cut(s) 299, 449, 617
NdeI CATATG 1 cut(s) 516
NdeII GATC 4 cut(s) 4, 71, 123, 313
NlaIII CATG 1 cut(s) 493
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 420
NmuCI GTSAC 2 cut(s) 272, 527
OliI CACNNNNGTG 1 cut(s) 339
PaeR7I CTCGAG 1 cut(s) 268
PcsI WCGNNNNNNNCGW 1 cut(s) 440
PdmI GAANNNNTTC 1 cut(s) 330
PfeI GAWTC 2 cut(s) 48, 204
PflMI CCANNNNNTGG 1 cut(s) 572
PkrI GCNGC 3 cut(s) 181, 304, 462
PleI GAGTC 2 cut(s) 279, 524
PpsI GAGTC 2 cut(s) 279, 524
PspN4I GGNNCC 1 cut(s) 6
PspXI VCTCGAGB 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 3 cut(s) 12, 456, 558
SatI GCNGC 3 cut(s) 180, 303, 461
Sau3AI GATC 4 cut(s) 4, 71, 123, 313
SchI GAGTC 2 cut(s) 280, 524
SetI ASST 6 cut(s) 86, 105, 269, 304, 500, 613
Sfr274I CTCGAG 1 cut(s) 268
SlaI CTCGAG 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 1 cut(s) 268
SmoI CTYRAG 1 cut(s) 268
Sse9I AATT 7 cut(s) 9, 55, 228, 234, 326, 590, 633
SsiI CCGC 2 cut(s) 452, 461
SspMI CTAG 2 cut(s) 621, 664
TaaI ACNGT 1 cut(s) 96
TaqI TCGA 2 cut(s) 99, 269
TasI AATT 7 cut(s) 9, 55, 228, 234, 326, 590, 633
TauI GCSGC 1 cut(s) 463
TfiI GAWTC 2 cut(s) 48, 204
Tru1I TTAA 3 cut(s) 12, 456, 558
Tru9I TTAA 3 cut(s) 12, 456, 558
TscAI CASTG 1 cut(s) 189
TseFI GTSAC 2 cut(s) 272, 527
TseI GCWGC 2 cut(s) 179, 302
Tsp45I GTSAC 2 cut(s) 272, 527
TspDTI ATGAA 4 cut(s) 205, 506, 604, 660
TspRI CASTG 1 cut(s) 189
Van91I CCANNNNNTGG 1 cut(s) 572
XapI RAATTY 3 cut(s) 55, 326, 633
XhoI CTCGAG 1 cut(s) 268
XmnI GAANNNNTTC 1 cut(s) 330
XspI CTAG 2 cut(s) 621, 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.