MD05G1064000.v1.1

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014184
Physical Location & Seq
Forward (+)
11253706 .. 11254505
800 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1064000.v1.1.491

Sequence Viewer

Length: 684 bp
ATGGATTCAATCAATTTTTATGACATCAAAGCTGAGAAGGCAAACGCAATCTTGAAATACCGCCAGCTTCGAAAAATCGCAAAACTCTTTCGGTTGATAGAGGTGCTTGCTGTTCTGCTCCTGCTTTCCAGGTTTTCTGTCCAACTCCCTCAGGCTGTCAAGAACTCTGCCTCTTACTTCAAAGACATATCCGGCTTCATGGTAAGTCCTCGCTTCGTGTTTGTCGTTGGAAATGTAATAGTTATCATTCTCTACGCCAAGTCGGGCAGATTTTCAGCTAAAGACTCTAGCACAGACAGCTCGGGTGGTGATCTTTACGAAGAGTTTGTTCAGAACAGCGAAAAGAATCAAAAACTTCGTGGCGATGGGATTGAGTACCAGGCCATAAAAAAAAGGCCCGAGGATAGTATAGTTTCTGAGCAAGCTCCTCATACTTCGGAAGTCAAGAAGTTCACAAGGAGTCAGTCAGAGAATTTAGAACGCGCGGTTTGCAAGAGCTCGCGGCACTTACTGAAACGATCAGAGACGGAGAAATTCAAGGAAAATGTTCAGTCTGGCGGGAAATTGGTAGGGAGCGAGTACCCCGAAGACGGTATGAGTAACGAGGAGTTTCGAAGCAAGGTTGAGTCCTTCATTGCAAGACAGCAGAAGTTTCGACTGCAAGAAGAGTTCTCTGTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408

Protein Analysis

228

Amino Acids

25.9

Weight (kDa)

9.35

Isoelectric Point (pI)

60.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34560
fragaria_vesca FvH4_2g09200
malus_domestica MD05G1064000.v1.1 MD10G1072700.v1.1
prunus_persica Prupe.6G200500_v2.0.a1 Prupe.8G094100_v2.0.a1
pyrus_communis pycom10g05740
rosa_chinensis RchiOBHm_Chr6g0265051
rosa_laevigata RLG00000014213
rosa_roxburghii Rroxscaffold_175G00432120 Rroxscaffold_7G00203000
rosa_rugosa Rorug06G0013400
rosa_samantha Rh6AG136400 Rh6BG133400 Rh6CG131800 Rh6DG119100
rosa_wichuraiana Rw6G011770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 483, 485, 502
AciI CCGC 4 cut(s) 61, 485, 502, 558
AcsI RAATTY 2 cut(s) 472, 533
AfaI GTAC 2 cut(s) 377, 581
AfiI CCNNNNNNNGG 1 cut(s) 590
AgsI TTSAA 4 cut(s) 9, 55, 181, 538
AjnI CCWGG 2 cut(s) 128, 378
AluBI AGCT 6 cut(s) 32, 67, 278, 300, 425, 498
AluI AGCT 6 cut(s) 32, 67, 278, 300, 425, 498
Alw21I GWGCWC 1 cut(s) 500
Alw26I GTCTC 1 cut(s) 518
Ama87I CYCGRG 2 cut(s) 301, 398
AoxI GGCC 2 cut(s) 381, 395
ApoI RAATTY 2 cut(s) 472, 533
Asp700I GAANNNNTTC 1 cut(s) 546
AspLEI GCGC 1 cut(s) 485
AspS9I GGNCC 1 cut(s) 396
AsuHPI GGTGA 1 cut(s) 320
AsuII TTCGAA 2 cut(s) 70, 613
AvaI CYCGRG 2 cut(s) 301, 398
AxyI CCTNAGG 1 cut(s) 150
BanII GRGCYC 1 cut(s) 500
BbsI GAAGAC 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 500
BccI CCATC 1 cut(s) 359
BcgI CGANNNNNNTGC 2 cut(s) 635, 669
BciT130I CCWGG 2 cut(s) 130, 380
BcoDI GTCTC 1 cut(s) 518
BfaI CTAG 2 cut(s) 288, 682
BisI GCNGC 1 cut(s) 503
BlsI GCNGC 1 cut(s) 504
Bme1390I CCNGG 2 cut(s) 130, 380
BmeT110I CYCGRG 2 cut(s) 301, 398
BmgT120I GGNCC 1 cut(s) 396
BmrFI CCNGG 2 cut(s) 130, 380
BpiI GAAGAC 1 cut(s) 594
Bpu14I TTCGAA 2 cut(s) 70, 613
BsaJI CCNNGG 1 cut(s) 399
Bsc4I CCNNNNNNNGG 1 cut(s) 590
Bse21I CCTNAGG 1 cut(s) 150
Bse3DI GCAATG 1 cut(s) 633
BseBI CCWGG 2 cut(s) 130, 380
BseDI CCNNGG 1 cut(s) 399
BseLI CCNNNNNNNGG 1 cut(s) 590
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 3 cut(s) 24, 164, 408
BseRI GAGGAG 2 cut(s) 417, 620
Bsh1236I CGCG 3 cut(s) 483, 485, 502
BshFI GGCC 2 cut(s) 383, 397
BsiHKAI GWGCWC 1 cut(s) 500
BsiHKCI CYCGRG 2 cut(s) 301, 398
BsiSI CCGG 1 cut(s) 192
BslI CCNNNNNNNGG 1 cut(s) 590
BsmAI GTCTC 1 cut(s) 518
BsmBI CGTCTC 1 cut(s) 518
BsnI GGCC 2 cut(s) 383, 397
BsoBI CYCGRG 2 cut(s) 301, 398
Bsp119I TTCGAA 2 cut(s) 70, 613
Bsp1286I GDGCHC 1 cut(s) 500
Bsp143I GATC 2 cut(s) 310, 518
BspACI CCGC 4 cut(s) 61, 485, 502, 558
BspANI GGCC 2 cut(s) 383, 397
BspCNI CTCAG 3 cut(s) 25, 163, 409
BspFNI CGCG 3 cut(s) 483, 485, 502
BspT104I TTCGAA 2 cut(s) 70, 613
BsrDI GCAATG 1 cut(s) 633
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 2 cut(s) 310, 518
Bst2UI CCWGG 2 cut(s) 130, 380
Bst4CI ACNGT 1 cut(s) 593
Bst6I CTCTTC 2 cut(s) 315, 660
BstBI TTCGAA 2 cut(s) 70, 613
BstC8I GCNNGC 4 cut(s) 65, 108, 423, 500
BstDEI CTNAG 3 cut(s) 33, 150, 417
BstFNI CGCG 3 cut(s) 483, 485, 502
BstHHI GCGC 1 cut(s) 485
BstKTI GATC 2 cut(s) 313, 521
BstMAI GTCTC 1 cut(s) 518
BstMBI GATC 2 cut(s) 310, 518
BstMWI GCNNNNNNNGC 3 cut(s) 38, 297, 489
BstNI CCWGG 2 cut(s) 130, 380
BstSCI CCNGG 2 cut(s) 128, 378
BstUI CGCG 3 cut(s) 483, 485, 502
BstV2I GAAGAC 1 cut(s) 594
Bsu36I CCTNAGG 1 cut(s) 150
BsuRI GGCC 2 cut(s) 383, 397
BtgZI GCGATG 1 cut(s) 378
Cac8I GCNNGC 4 cut(s) 65, 108, 423, 500
CfoI GCGC 1 cut(s) 485
Cfr13I GGNCC 1 cut(s) 396
Csp6I GTAC 2 cut(s) 376, 580
CviAII CATG 1 cut(s) 199
CviQI GTAC 2 cut(s) 376, 580
DdeI CTNAG 3 cut(s) 33, 150, 417
DpnI GATC 2 cut(s) 312, 520
DpnII GATC 2 cut(s) 310, 518
Eam1104I CTCTTC 2 cut(s) 315, 660
EarI CTCTTC 2 cut(s) 315, 660
Ecl136II GAGCTC 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 500
Eco53kI GAGCTC 1 cut(s) 498
Eco81I CCTNAGG 1 cut(s) 150
Eco88I CYCGRG 2 cut(s) 301, 398
EcoICRI GAGCTC 1 cut(s) 498
EcoRII CCWGG 2 cut(s) 128, 378
EcoT38I GRGCYC 1 cut(s) 500
Esp3I CGTCTC 1 cut(s) 518
FaeI CATG 1 cut(s) 202
FaiI YATR 7 cut(s) 21, 188, 200, 386, 410, 432, 596
FatI CATG 1 cut(s) 198
FauI CCCGC 1 cut(s) 551
Fnu4HI GCNGC 1 cut(s) 503
FriOI GRGCYC 1 cut(s) 500
Fsp4HI GCNGC 1 cut(s) 503
FspBI CTAG 2 cut(s) 288, 682
GlaI GCGC 1 cut(s) 484
GluI GCNGC 1 cut(s) 503
HaeIII GGCC 2 cut(s) 383, 397
HapII CCGG 1 cut(s) 192
HhaI GCGC 1 cut(s) 485
Hin1II CATG 1 cut(s) 202
Hin6I GCGC 1 cut(s) 483
HinP1I GCGC 1 cut(s) 483
HinfI GANTC 5 cut(s) 5, 284, 346, 460, 626
HpaII CCGG 1 cut(s) 192
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 2 cut(s) 453, 679
Hpy188I TCNGA 5 cut(s) 333, 418, 439, 469, 523
Hpy188III TCNNGA 3 cut(s) 52, 160, 445
Hpy8I GTNNAC 2 cut(s) 453, 679
HpyAV CCTTC 2 cut(s) 31, 640
HpyCH4III ACNGT 1 cut(s) 593
HpyCH4V TGCA 3 cut(s) 492, 638, 661
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 297, 489
HpyF3I CTNAG 3 cut(s) 33, 150, 417
Hsp92II CATG 1 cut(s) 202
HspAI GCGC 1 cut(s) 483
Kzo9I GATC 2 cut(s) 310, 518
LmnI GCTCC 3 cut(s) 123, 430, 573
LpnPI CCDG 9 cut(s) 77, 115, 134, 137, 142, 205, 365, 392, 540
MaeI CTAG 2 cut(s) 288, 682
MaeIII GTNAC 1 cut(s) 599
MalI GATC 2 cut(s) 312, 520
MboI GATC 2 cut(s) 310, 518
MboII GAAGA 3 cut(s) 332, 599, 677
MhlI GDGCHC 1 cut(s) 500
MluCI AATT 4 cut(s) 13, 472, 533, 563
MlyI GAGTC 3 cut(s) 278, 469, 635
MmeI TCCRAC 2 cut(s) 166, 208
MnlI CCTC 7 cut(s) 94, 159, 181, 219, 394, 438, 598
MroXI GAANNNNTTC 1 cut(s) 546
MspI CCGG 1 cut(s) 192
MspR9I CCNGG 2 cut(s) 130, 380
MvaI CCWGG 2 cut(s) 130, 380
MvnI CGCG 3 cut(s) 483, 485, 502
MwoI GCNNNNNNNGC 3 cut(s) 38, 297, 489
NdeII GATC 2 cut(s) 310, 518
NlaIII CATG 1 cut(s) 202
NspV TTCGAA 2 cut(s) 70, 613
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdmI GAANNNNTTC 1 cut(s) 546
PfeI GAWTC 2 cut(s) 5, 346
PkrI GCNGC 1 cut(s) 504
PleI GAGTC 3 cut(s) 278, 468, 634
PpsI GAGTC 3 cut(s) 278, 468, 634
Psp124BI GAGCTC 1 cut(s) 500
Psp6I CCWGG 2 cut(s) 128, 378
PspGI CCWGG 2 cut(s) 128, 378
PspPI GGNCC 1 cut(s) 396
RsaI GTAC 2 cut(s) 377, 581
RsaNI GTAC 2 cut(s) 376, 580
SacI GAGCTC 1 cut(s) 500
SatI GCNGC 1 cut(s) 503
Sau3AI GATC 2 cut(s) 310, 518
Sau96I GGNCC 1 cut(s) 396
SchI GAGTC 3 cut(s) 278, 469, 635
ScrFI CCNGG 2 cut(s) 130, 380
SduI GDGCHC 1 cut(s) 500
SetI ASST 9 cut(s) 34, 69, 105, 134, 280, 302, 427, 500, 624
SfuI TTCGAA 2 cut(s) 70, 613
Sse9I AATT 4 cut(s) 13, 472, 533, 563
SsiI CCGC 4 cut(s) 61, 485, 502, 558
SspMI CTAG 2 cut(s) 288, 682
SstI GAGCTC 1 cut(s) 500
StyD4I CCNGG 2 cut(s) 128, 378
TaaI ACNGT 1 cut(s) 593
TaqI TCGA 3 cut(s) 70, 613, 655
TasI AATT 4 cut(s) 13, 472, 533, 563
TauI GCSGC 1 cut(s) 505
TfiI GAWTC 2 cut(s) 5, 346
TspDTI ATGAA 2 cut(s) 187, 622
TspGWI ACGGA 1 cut(s) 542
XapI RAATTY 2 cut(s) 472, 533
XmnI GAANNNNTTC 1 cut(s) 546
XspI CTAG 2 cut(s) 288, 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.