Rorug06G0013400

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0014184
Physical Location & Seq
Forward (+)
1781096 .. 1781956
861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0013400.1

Sequence Viewer

Length: 861 bp
ATGCTGTCTTATTATTATACTGATCTATTGTTCATCATTGATCGATTTATTAGCGTTCTGCATTTATATTTGGTTTGGCCTACAAAGTTCTGGAATGTAACTGTCGCGATCGGTTTCTGCGCATTTTCAACTATAGGTTTCTGCCCCATTTTCAACTATATTCAGTGCTCTCAACATATATTATTATTACGATTTCATGTTTTCAACTCCAACAAACAAGGTCAACAATTAGAGAAACAGAAGGTGAGGAAGATATTCCACTCAATCCAGAAAAGTCTTCTCAAATTAAAGAAGAAGAGGGAGAAGAAAAAAGAAGAAGATATCAAATGGGAAGAGAAGGAGGATTTGTTCTGTAGTATTCGAGCTCCTCCCTTGGTTCAGGAGAATAAAGATGAGGACTTGGAAATGTTGGCAAGGCGTCGAGCTTATCGAGGAGATTGCCGCAAATGGAAGCAGGAGAAGATCTCTATAGCTGCAAAACTTGACTCTATGATTGCAGAAGCAACTGCTGCTTCTGCAAAGCTTTTGGCTGCTGCAGAGGATAACTGTGATTATATAATTACTACCGAGCTTGTTTGCGTGAGTAGAGAAAAATGCAATGATCTTCATGACACGATTGAGAAAAAAACTAATTATGTTCGCCACTCCAGAATCCTATGGTACCGTCTTTATGTAATGCTTGAGGCTGAATCCAAGTCGTCTAATTTGTTTCTTGAACTTGTAGAAGAGTGGGAAGAGAAATGCTGGGCATTTGAGGAAGACAAGGACAATCTCATTGAAAAGCGTTGCAGCACAAAGAAGCGTATTGAGCGAGAACCAGCTTATACTAATTTTGCAATTGGAAGCATCTTCCGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408

Protein Analysis

286

Amino Acids

33.98

Weight (kDa)

8.55

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34560
fragaria_vesca FvH4_2g09200
malus_domestica MD05G1064000.v1.1 MD10G1072700.v1.1
prunus_persica Prupe.6G200500_v2.0.a1 Prupe.8G094100_v2.0.a1
pyrus_communis pycom10g05740
rosa_chinensis RchiOBHm_Chr6g0265051
rosa_laevigata RLG00000014213
rosa_roxburghii Rroxscaffold_175G00432120 Rroxscaffold_7G00203000
rosa_rugosa Rorug06G0013400
rosa_samantha Rh6AG136400 Rh6BG133400 Rh6CG131800 Rh6DG119100
rosa_wichuraiana Rw6G011770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 121
Acc65I GGTACC 1 cut(s) 660
AccB1I GGYRCC 1 cut(s) 660
AccII CGCG 1 cut(s) 107
AciI CCGC 1 cut(s) 442
AcyI GRCGYC 1 cut(s) 418
AfaI GTAC 1 cut(s) 662
AgsI TTSAA 5 cut(s) 129, 154, 205, 716, 779
AloI GAACNNNNNNTCC 2 cut(s) 332, 364
AluBI AGCT 6 cut(s) 365, 425, 473, 523, 571, 821
AluI AGCT 6 cut(s) 365, 425, 473, 523, 571, 821
Alw21I GWGCWC 2 cut(s) 170, 367
AoxI GGCC 1 cut(s) 77
ApeKI GCWGC 5 cut(s) 473, 509, 530, 533, 789
Asp700I GAANNNNTTC 1 cut(s) 254
Asp718I GGTACC 1 cut(s) 660
AspLEI GCGC 1 cut(s) 122
AsuHPI GGTGA 1 cut(s) 256
BanI GGYRCC 1 cut(s) 660
BanII GRGCYC 1 cut(s) 367
BbsI GAAGAC 2 cut(s) 269, 765
Bbv12I GWGCWC 2 cut(s) 170, 367
BbvI GCAGC 5 cut(s) 460, 496, 517, 520, 801
BcgI CGANNNNNNTGC 2 cut(s) 420, 454
BfmI CTRYAG 4 cut(s) 132, 352, 468, 534
BglII AGATCT 1 cut(s) 462
BisI GCNGC 6 cut(s) 442, 474, 510, 531, 534, 790
BlsI GCNGC 6 cut(s) 443, 475, 511, 532, 535, 791
BmiI GGNNCC 1 cut(s) 662
BmsI GCATC 1 cut(s) 855
BpiI GAAGAC 2 cut(s) 269, 765
BplI GAGNNNNNCTC 2 cut(s) 449, 481
BpmI CTGGAG 1 cut(s) 631
BpuEI CTTGAG 1 cut(s) 701
Bsa29I ATCGAT 1 cut(s) 43
BsaHI GRCGYC 1 cut(s) 418
BsaJI CCNNGG 1 cut(s) 372
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bse3DI GCAATG 1 cut(s) 604
BseCI ATCGAT 1 cut(s) 43
BseDI CCNNGG 1 cut(s) 372
BseMI GCAATG 1 cut(s) 604
BseRI GAGGAG 2 cut(s) 357, 447
BseXI GCAGC 5 cut(s) 460, 496, 517, 520, 801
BseYI CCCAGC 1 cut(s) 744
Bsh1236I CGCG 1 cut(s) 107
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 1 cut(s) 79
BshNI GGYRCC 1 cut(s) 660
BshVI ATCGAT 1 cut(s) 43
BsiEI CGRYCG 1 cut(s) 111
BsiHKAI GWGCWC 2 cut(s) 170, 367
BsnI GGCC 1 cut(s) 79
Bsp1286I GDGCHC 2 cut(s) 170, 367
Bsp143I GATC 5 cut(s) 22, 40, 108, 462, 601
Bsp68I TCGCGA 1 cut(s) 107
BspACI CCGC 1 cut(s) 442
BspANI GGCC 1 cut(s) 79
BspDI ATCGAT 1 cut(s) 43
BspFNI CGCG 1 cut(s) 107
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 1 cut(s) 662
BspMAI CTGCAG 1 cut(s) 538
BspT107I GGYRCC 1 cut(s) 660
BsrDI GCAATG 1 cut(s) 604
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 5 cut(s) 22, 40, 108, 462, 601
BssNI GRCGYC 1 cut(s) 418
BssT1I CCWWGG 1 cut(s) 372
Bst4CI ACNGT 3 cut(s) 103, 548, 665
Bst6I CTCTTC 4 cut(s) 290, 327, 720, 729
BstACI GRCGYC 1 cut(s) 418
BstAPI GCANNNNNTGC 1 cut(s) 509
BstFNI CGCG 1 cut(s) 107
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 5 cut(s) 25, 43, 111, 465, 604
BstMBI GATC 5 cut(s) 22, 40, 108, 462, 601
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 3 cut(s) 509, 515, 808
BstSFI CTRYAG 4 cut(s) 132, 352, 468, 534
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 5 cut(s) 460, 496, 517, 520, 801
BstV2I GAAGAC 2 cut(s) 269, 765
BstX2I RGATCY 1 cut(s) 462
BstYI RGATCY 1 cut(s) 462
Bsu15I ATCGAT 1 cut(s) 43
BsuRI GGCC 1 cut(s) 79
BsuTUI ATCGAT 1 cut(s) 43
BtsIMutI CAGTG 1 cut(s) 170
BtuMI TCGCGA 1 cut(s) 107
CciI TCATGA 1 cut(s) 607
CfoI GCGC 1 cut(s) 122
ClaI ATCGAT 1 cut(s) 43
CseI GACGC 1 cut(s) 407
Csp6I GTAC 1 cut(s) 661
CviAII CATG 2 cut(s) 197, 608
CviJI RGCY 9 cut(s) 79, 365, 425, 473, 523, 530, 571, 686, 821
CviKI_1 RGCY 9 cut(s) 79, 365, 425, 473, 523, 530, 571, 686, 821
CviQI GTAC 1 cut(s) 661
DpnI GATC 5 cut(s) 24, 42, 110, 464, 603
DpnII GATC 5 cut(s) 22, 40, 108, 462, 601
Eam1104I CTCTTC 4 cut(s) 290, 327, 720, 729
EarI CTCTTC 4 cut(s) 290, 327, 720, 729
Ecl136II GAGCTC 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 372
Eco24I GRGCYC 1 cut(s) 367
Eco32I GATATC 1 cut(s) 322
Eco53kI GAGCTC 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 365
EcoRV GATATC 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 372
EcoT38I GRGCYC 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 2 cut(s) 200, 611
FatI CATG 2 cut(s) 196, 607
Fnu4HI GCNGC 6 cut(s) 442, 474, 510, 531, 534, 790
FriOI GRGCYC 1 cut(s) 367
Fsp4HI GCNGC 6 cut(s) 442, 474, 510, 531, 534, 790
FspI TGCGCA 1 cut(s) 121
GlaI GCGC 1 cut(s) 121
GluI GCNGC 6 cut(s) 442, 474, 510, 531, 534, 790
GsaI CCCAGC 1 cut(s) 748
GsuI CTGGAG 1 cut(s) 631
HaeIII GGCC 1 cut(s) 79
HgaI GACGC 1 cut(s) 407
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 418
Hin1II CATG 2 cut(s) 200, 611
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HincII GTYRAC 1 cut(s) 224
HindII GTYRAC 1 cut(s) 224
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 3 cut(s) 485, 651, 689
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 1 cut(s) 224
Hpy188III TCNNGA 7 cut(s) 91, 106, 268, 380, 608, 648, 713
Hpy8I GTNNAC 1 cut(s) 224
Hpy99I CGWCG 1 cut(s) 423
HpyAV CCTTC 2 cut(s) 235, 331
HpyCH4III ACNGT 3 cut(s) 103, 548, 665
HpyCH4V TGCA 8 cut(s) 61, 476, 497, 518, 536, 597, 789, 836
HpyF10VI GCNNNNNNNGC 3 cut(s) 509, 515, 808
Hsp92I GRCGYC 1 cut(s) 418
Hsp92II CATG 2 cut(s) 200, 611
HspAI GCGC 1 cut(s) 120
KpnI GGTACC 1 cut(s) 664
Kzo9I GATC 5 cut(s) 22, 40, 108, 462, 601
LmnI GCTCC 1 cut(s) 370
LpnPI CCDG 7 cut(s) 76, 281, 365, 440, 661, 730, 831
Lsp1109I GCAGC 5 cut(s) 460, 496, 517, 520, 801
LweI GCATC 1 cut(s) 855
MaeIII GTNAC 2 cut(s) 97, 854
MalI GATC 5 cut(s) 24, 42, 110, 464, 603
MboI GATC 5 cut(s) 22, 40, 108, 462, 601
MfeI CAATTG 1 cut(s) 837
MflI RGATCY 1 cut(s) 462
MhlI GDGCHC 2 cut(s) 170, 367
MluCI AATT 7 cut(s) 227, 284, 558, 631, 703, 829, 837
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 9 cut(s) 240, 291, 334, 378, 388, 425, 532, 676, 748
MroXI GAANNNNTTC 1 cut(s) 254
MseI TTAA 1 cut(s) 287
MunI CAATTG 1 cut(s) 837
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 3 cut(s) 509, 515, 808
NdeII GATC 5 cut(s) 22, 40, 108, 462, 601
NlaIII CATG 2 cut(s) 200, 611
NlaIV GGNNCC 1 cut(s) 662
NmuCI GTSAC 1 cut(s) 854
NruI TCGCGA 1 cut(s) 107
NsbI TGCGCA 1 cut(s) 121
PagI TCATGA 1 cut(s) 607
PcsI WCGNNNNNNNCGW 1 cut(s) 427
PdmI GAANNNNTTC 1 cut(s) 254
PfeI GAWTC 2 cut(s) 651, 689
PkrI GCNGC 6 cut(s) 443, 475, 511, 532, 535, 791
Ple19I CGATCG 1 cut(s) 111
PleI GAGTC 1 cut(s) 479
PpsI GAGTC 1 cut(s) 479
Psp124BI GAGCTC 1 cut(s) 367
PspFI CCCAGC 1 cut(s) 744
PspN4I GGNNCC 1 cut(s) 662
PstI CTGCAG 1 cut(s) 538
PsuI RGATCY 1 cut(s) 462
PvuI CGATCG 1 cut(s) 111
RruI TCGCGA 1 cut(s) 107
RsaI GTAC 1 cut(s) 662
RsaNI GTAC 1 cut(s) 661
SacI GAGCTC 1 cut(s) 367
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 6 cut(s) 442, 474, 510, 531, 534, 790
Sau3AI GATC 5 cut(s) 22, 40, 108, 462, 601
SchI GAGTC 1 cut(s) 479
SduI GDGCHC 2 cut(s) 170, 367
SetI ASST 9 cut(s) 139, 223, 246, 367, 427, 475, 525, 573, 823
SfaNI GCATC 1 cut(s) 855
SfcI CTRYAG 4 cut(s) 132, 352, 468, 534
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
Sse9I AATT 7 cut(s) 227, 284, 558, 631, 703, 829, 837
SsiI CCGC 1 cut(s) 442
SstI GAGCTC 1 cut(s) 367
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 3 cut(s) 103, 548, 665
TaqI TCGA 4 cut(s) 43, 361, 421, 430
TasI AATT 7 cut(s) 227, 284, 558, 631, 703, 829, 837
TauI GCSGC 1 cut(s) 444
TfiI GAWTC 2 cut(s) 651, 689
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TscAI CASTG 1 cut(s) 170
TseFI GTSAC 1 cut(s) 854
TseI GCWGC 5 cut(s) 473, 509, 530, 533, 789
Tsp45I GTSAC 1 cut(s) 854
TspDTI ATGAA 3 cut(s) 22, 185, 596
TspGWI ACGGA 1 cut(s) 842
TspRI CASTG 1 cut(s) 170
XmnI GAANNNNTTC 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.