AT4G35750

Ganglioside-induced differentiation-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015291
Physical Location & Seq
Reverse (-)
16940462 .. 16942060
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35750.1

Sequence Viewer

Length: 609 bp
ATGAGTTCTCAGATTTCGGAGATTGAACAAGAGCAGCTGATCGAGAAGCTTGAGATCTTCAAGATCCATGGCAGAGACAAACGTGGCCGTAAGATCCTTCGTATTATCGGAAAATTCTTCCCAGCTCGATTTCTGTCACTGGATGTGTTGAAGAAGTATCTAGAGGAGAAGATATTTCCTCGATTAGGTAGAAAACCATTCGCCGTACTCTACGTCCACACCGGCGTACAGAGAAGCGAGAACTTCCCAGGTATCTCAGCTCTACGAGCGATCTACGACGCAATTCCGGTAAACGTCAGAGACAATCTTCAGGAGGTTTACTTCCTCCATCCAGGTCTTCAATCACGTCTCTTCCTCGCCACCTGCGGCCGATTTCTATTTTCCGGCGGGTTGTACGGGAAGCTGAGGTACATAAGCAGAGTTGATTATCTGTGGGAACATGTGAGGAGGAATGAGATAGAGATGCCGGAGTTTGTATACGATCACGATGATGATCTGGAGTATCGTCCGATGATGGATTACGGTCAAGAAAGCGATCACGCGAGGGTTTTCGCCGGAGCCGCCGTGGATTCATCAGTCTCAAGTTTCTCCATGAGGTGTATCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2

Protein Analysis

202

Amino Acids

23.58

Weight (kDa)

8.48

Isoelectric Point (pI)

53.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_2 PF13716 29 - 165 2.9e-40 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015291)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35750
fragaria_vesca FvH4_1g10910
malus_domestica MD02G1122200.v1.1 MD15G1236100.v1.1
prunus_persica Prupe.7G176400_v2.0.a1
pyrus_communis pycom02g09510 pycom15g21010
rosa_chinensis RchiOBHm_Chr2g0098171
rosa_laevigata RLG00000016768
rosa_multiflora Rmu_sc0009178.1_g000010
rosa_roxburghii Rroxscaffold_2G00144230
rosa_rugosa Rorug02G0070700
rosa_samantha Rh2BG121000 Rh2CG122600 Rh2DG122500
rosa_wichuraiana Rw2G009230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 371
Acc36I ACCTGC 1 cut(s) 371
AccI GTMKAC 1 cut(s) 477
AccII CGCG 1 cut(s) 542
AciI CCGC 3 cut(s) 366, 387, 561
AclWI GGATC 2 cut(s) 58, 88
AcoI YGGCCR 2 cut(s) 85, 367
AcsI RAATTY 1 cut(s) 113
AcuI CTGAAG 1 cut(s) 293
AfaI GTAC 4 cut(s) 207, 228, 395, 410
AfiI CCNNNNNNNGG 1 cut(s) 185
AflIII ACRYGT 1 cut(s) 439
AgsI TTSAA 4 cut(s) 26, 61, 151, 341
AjiI CACGTC 1 cut(s) 347
AjnI CCWGG 2 cut(s) 247, 331
AluBI AGCT 5 cut(s) 37, 49, 125, 260, 403
AluI AGCT 5 cut(s) 37, 49, 125, 260, 403
Alw26I GTCTC 4 cut(s) 69, 294, 353, 583
AlwI GGATC 2 cut(s) 58, 88
AoxI GGCC 2 cut(s) 85, 367
ApeKI GCWGC 1 cut(s) 34
ApoI RAATTY 1 cut(s) 113
BarI GAAGNNNNNNTAC 2 cut(s) 392, 424
BbsI GAAGAC 1 cut(s) 329
BbvCI CCTCAGC 1 cut(s) 404
BbvI GCAGC 1 cut(s) 46
BccI CCATC 2 cut(s) 336, 508
BceAI ACGGC 3 cut(s) 72, 188, 548
BciT130I CCWGG 2 cut(s) 249, 333
BcoDI GTCTC 4 cut(s) 69, 294, 353, 583
BfaI CTAG 1 cut(s) 161
BfuAI ACCTGC 1 cut(s) 371
BglII AGATCT 1 cut(s) 54
BisI GCNGC 3 cut(s) 35, 367, 561
BlsI GCNGC 3 cut(s) 36, 368, 562
Bme1390I CCNGG 2 cut(s) 249, 333
BmgBI CACGTC 1 cut(s) 347
BmiI GGNNCC 1 cut(s) 559
BmrFI CCNGG 2 cut(s) 249, 333
BmsI GCATC 1 cut(s) 453
BpiI GAAGAC 1 cut(s) 329
BpmI CTGGAG 1 cut(s) 518
Bpu10I CCTNAGC 1 cut(s) 404
BpuEI CTTGAG 2 cut(s) 71, 565
BsaBI GATNNNNATC 1 cut(s) 492
BsaJI CCNNGG 3 cut(s) 67, 247, 564
BsaWI WCCGGW 1 cut(s) 286
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 1 cut(s) 144
Bse8I GATNNNNATC 1 cut(s) 492
BseBI CCWGG 2 cut(s) 249, 333
BseDI CCNNGG 3 cut(s) 67, 247, 564
BseGI GGATG 2 cut(s) 148, 328
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMII CTCAG 3 cut(s) 23, 270, 395
BseNI ACTGG 1 cut(s) 144
BseRI GAGGAG 2 cut(s) 179, 460
BseX3I CGGCCG 1 cut(s) 367
BseXI GCAGC 1 cut(s) 46
BseYI CCCAGC 1 cut(s) 121
Bsh1236I CGCG 1 cut(s) 542
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 2 cut(s) 87, 369
BsiEI CGRYCG 1 cut(s) 370
BsiSI CCGG 5 cut(s) 222, 287, 384, 467, 555
BslI CCNNNNNNNGG 1 cut(s) 185
BsmAI GTCTC 4 cut(s) 69, 294, 353, 583
BsmBI CGTCTC 1 cut(s) 353
BsnI GGCC 2 cut(s) 87, 369
Bsp143I GATC 8 cut(s) 39, 54, 63, 93, 270, 481, 493, 535
Bsp19I CCATGG 1 cut(s) 67
BspACI CCGC 3 cut(s) 366, 387, 561
BspANI GGCC 2 cut(s) 87, 369
BspCNI CTCAG 3 cut(s) 22, 269, 396
BspFNI CGCG 1 cut(s) 542
BspLI GGNNCC 1 cut(s) 559
BspMI ACCTGC 1 cut(s) 371
BspPI GGATC 2 cut(s) 58, 88
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 1 cut(s) 144
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 3 cut(s) 67, 247, 564
BssMI GATC 8 cut(s) 39, 54, 63, 93, 270, 481, 493, 535
BssNAI GTATAC 1 cut(s) 478
BssT1I CCWWGG 1 cut(s) 67
Bst1107I GTATAC 1 cut(s) 478
Bst2UI CCWGG 2 cut(s) 249, 333
Bst4CI ACNGT 1 cut(s) 524
Bst6I CTCTTC 1 cut(s) 356
BstDEI CTNAG 3 cut(s) 9, 256, 404
BstDSI CCRYGG 2 cut(s) 67, 564
BstENI CCTNNNNNAGG 1 cut(s) 183
BstF5I GGATG 2 cut(s) 148, 328
BstFNI CGCG 1 cut(s) 542
BstKTI GATC 8 cut(s) 42, 57, 66, 96, 273, 484, 496, 538
BstMAI GTCTC 4 cut(s) 69, 294, 353, 583
BstMBI GATC 8 cut(s) 39, 54, 63, 93, 270, 481, 493, 535
BstMCI CGRYCG 1 cut(s) 370
BstMWI GCNNNNNNNGC 2 cut(s) 266, 560
BstNI CCWGG 2 cut(s) 249, 333
BstNSI RCATGY 1 cut(s) 443
BstSCI CCNGG 2 cut(s) 247, 331
BstUI CGCG 1 cut(s) 542
BstV1I GCAGC 1 cut(s) 46
BstV2I GAAGAC 1 cut(s) 329
BstX2I RGATCY 3 cut(s) 54, 63, 93
BstYI RGATCY 3 cut(s) 54, 63, 93
BstZ17I GTATAC 1 cut(s) 478
BstZI CGGCCG 1 cut(s) 367
BsuRI GGCC 2 cut(s) 87, 369
BtgI CCRYGG 2 cut(s) 67, 564
BtrI CACGTC 1 cut(s) 347
BtsCI GGATG 2 cut(s) 148, 328
BtsIMutI CAGTG 1 cut(s) 137
BveI ACCTGC 1 cut(s) 371
Cfr10I RCCGGY 1 cut(s) 221
CseI GACGC 1 cut(s) 287
Csp6I GTAC 4 cut(s) 206, 227, 394, 409
CviAII CATG 3 cut(s) 68, 440, 592
CviJI RGCY 8 cut(s) 37, 49, 87, 125, 260, 369, 403, 560
CviKI_1 RGCY 8 cut(s) 37, 49, 87, 125, 260, 369, 403, 560
CviQI GTAC 4 cut(s) 206, 227, 394, 409
DdeI CTNAG 3 cut(s) 9, 256, 404
DpnI GATC 8 cut(s) 41, 56, 65, 95, 272, 483, 495, 537
DpnII GATC 8 cut(s) 39, 54, 63, 93, 270, 481, 493, 535
EaeI YGGCCR 2 cut(s) 85, 367
EagI CGGCCG 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
EclXI CGGCCG 1 cut(s) 367
Eco130I CCWWGG 1 cut(s) 67
Eco52I CGGCCG 1 cut(s) 367
Eco57I CTGAAG 1 cut(s) 293
EcoNI CCTNNNNNAGG 1 cut(s) 183
EcoRII CCWGG 2 cut(s) 247, 331
EcoT14I CCWWGG 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 67
Esp3I CGTCTC 1 cut(s) 353
FaeI CATG 3 cut(s) 71, 443, 595
FaiI YATR 6 cut(s) 69, 413, 441, 478, 593, 607
FatI CATG 3 cut(s) 67, 439, 591
FauI CCCGC 1 cut(s) 380
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 3 cut(s) 35, 367, 561
FokI GGATG 2 cut(s) 155, 315
Fsp4HI GCNGC 3 cut(s) 35, 367, 561
FspBI CTAG 1 cut(s) 161
GluI GCNGC 3 cut(s) 35, 367, 561
GsaI CCCAGC 1 cut(s) 125
GsuI CTGGAG 1 cut(s) 518
HaeIII GGCC 2 cut(s) 87, 369
HapII CCGG 5 cut(s) 222, 287, 384, 467, 555
HgaI GACGC 1 cut(s) 287
Hin1II CATG 3 cut(s) 71, 443, 595
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 1 cut(s) 569
HpaII CCGG 5 cut(s) 222, 287, 384, 467, 555
Hpy166II GTNNAC 4 cut(s) 217, 292, 319, 478
Hpy188I TCNGA 5 cut(s) 12, 19, 110, 299, 510
Hpy188III TCNNGA 7 cut(s) 43, 61, 161, 311, 485, 497, 527
Hpy8I GTNNAC 4 cut(s) 217, 292, 319, 478
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 1 cut(s) 107
HpyCH4III ACNGT 1 cut(s) 524
HpyCH4IV ACGT 4 cut(s) 82, 213, 294, 346
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 560
HpyF3I CTNAG 3 cut(s) 9, 256, 404
HpySE526I ACGT 4 cut(s) 82, 213, 294, 346
Hsp92II CATG 3 cut(s) 71, 443, 595
Kzo9I GATC 8 cut(s) 39, 54, 63, 93, 270, 481, 493, 535
LmnI GCTCC 1 cut(s) 557
Lsp1109I GCAGC 1 cut(s) 46
LweI GCATC 1 cut(s) 453
MaeI CTAG 1 cut(s) 161
MaeII ACGT 4 cut(s) 82, 213, 294, 346
MaeIII GTNAC 1 cut(s) 135
MalI GATC 8 cut(s) 41, 56, 65, 95, 272, 483, 495, 537
MboI GATC 8 cut(s) 39, 54, 63, 93, 270, 481, 493, 535
MboII GAAGA 7 cut(s) 49, 109, 163, 181, 299, 329, 343
MflI RGATCY 3 cut(s) 54, 63, 93
MluCI AATT 2 cut(s) 113, 282
MslI CAYNNNNRTG 1 cut(s) 489
MspA1I CMGCKG 1 cut(s) 37
MspI CCGG 5 cut(s) 222, 287, 384, 467, 555
MspR9I CCNGG 2 cut(s) 249, 333
MvaI CCWGG 2 cut(s) 249, 333
MvnI CGCG 1 cut(s) 542
MwoI GCNNNNNNNGC 2 cut(s) 266, 560
NcoI CCATGG 1 cut(s) 67
NdeII GATC 8 cut(s) 39, 54, 63, 93, 270, 481, 493, 535
NlaIII CATG 3 cut(s) 71, 443, 595
NlaIV GGNNCC 1 cut(s) 559
NmuCI GTSAC 1 cut(s) 135
NspI RCATGY 1 cut(s) 443
PaqCI CACCTGC 1 cut(s) 371
PciI ACATGT 1 cut(s) 439
PfeI GAWTC 1 cut(s) 569
PkrI GCNGC 3 cut(s) 36, 368, 562
PscI ACATGT 1 cut(s) 439
Psp6I CCWGG 2 cut(s) 247, 331
PspFI CCCAGC 1 cut(s) 121
PspGI CCWGG 2 cut(s) 247, 331
PspN4I GGNNCC 1 cut(s) 559
PsuI RGATCY 3 cut(s) 54, 63, 93
PvuII CAGCTG 1 cut(s) 37
RsaI GTAC 4 cut(s) 207, 228, 395, 410
RsaNI GTAC 4 cut(s) 206, 227, 394, 409
RseI CAYNNNNRTG 1 cut(s) 489
SatI GCNGC 3 cut(s) 35, 367, 561
Sau3AI GATC 8 cut(s) 39, 54, 63, 93, 270, 481, 493, 535
ScrFI CCNGG 2 cut(s) 249, 333
SfaNI GCATC 1 cut(s) 453
SgrAI CRCCGGYG 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 2 cut(s) 50, 580
SmoI CTYRAG 2 cut(s) 50, 580
Sse9I AATT 2 cut(s) 113, 282
SsiI CCGC 3 cut(s) 366, 387, 561
SspMI CTAG 1 cut(s) 161
StyD4I CCNGG 2 cut(s) 247, 331
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 1 cut(s) 524
TaiI ACGT 4 cut(s) 85, 216, 297, 349
TaqI TCGA 3 cut(s) 42, 127, 181
TasI AATT 2 cut(s) 113, 282
TauI GCSGC 2 cut(s) 369, 563
TfiI GAWTC 1 cut(s) 569
TscAI CASTG 1 cut(s) 144
TseFI GTSAC 1 cut(s) 135
TseI GCWGC 1 cut(s) 34
Tsp45I GTSAC 1 cut(s) 135
TspDTI ATGAA 1 cut(s) 561
TspRI CASTG 1 cut(s) 144
XagI CCTNNNNNAGG 1 cut(s) 183
XapI RAATTY 1 cut(s) 113
XbaI TCTAGA 1 cut(s) 160
XceI RCATGY 1 cut(s) 443
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.