MD15G1236100.v1.1

Ganglioside-induced differentiation-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015291
Physical Location & Seq
Reverse (-)
19424121 .. 19425555
1435 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1236100.v1.1.491

Sequence Viewer

Length: 609 bp
ATGCATACTCAAATCTCCGATTCCGAGCAGCAGGAACTCATCGAGAAGCTCGACATCTTCAGGATCAGGGGACGTGACAAACGCGGCCGCACCGTCCTTCGCATCATCGGCAAATTCTTTCCAGCTCGAATTGTGAGCGTTGATGCTCTGCGGAAGTATTTGGAGGAGAGAATATTCCCAGAGATCGAGAAAAAGCCGTTCGCTGTGCTGTACGTGCACACTGGAGCTCAAAGGTGCGAGAACTTCCCCGGAATCTCAGCCGTCCGATCAATCTACGACGCCATCCCGATCGGCGTCCGCCGGAATCTCGAAGCCGTGTATTTCCTCCACCCCGGCCTCCAGGCCCGCCTCTTTTTCGCCACCTTCGGCCGCTTCTTTTTCACCGACGGGGTATACGGAAAGCTGAGGTACGTGAGCAGGCTCGATTATTTGTGGGAGCACGTGAGGAGGAGCGAGATTGAGGTGCCTGATTTCGTGTACGATCATGATGAGGACTTGGAGCACCGTCCGATGATGGACTACGGATTGGAGAGCGATCATCCGAGGGTGTACGATGCGCCCGCGGTTGATTCGCCTGTGTCGACGTACTCCATGAGATGCATCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2

Protein Analysis

203

Amino Acids

23.73

Weight (kDa)

6.66

Isoelectric Point (pI)

52.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_2 PF13716 29 - 165 2.7e-37 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015291)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35750
fragaria_vesca FvH4_1g10910
malus_domestica MD02G1122200.v1.1 MD15G1236100.v1.1
prunus_persica Prupe.7G176400_v2.0.a1
pyrus_communis pycom02g09510 pycom15g21010
rosa_chinensis RchiOBHm_Chr2g0098171
rosa_laevigata RLG00000016768
rosa_multiflora Rmu_sc0009178.1_g000010
rosa_roxburghii Rroxscaffold_2G00144230
rosa_rugosa Rorug02G0070700
rosa_samantha Rh2BG121000 Rh2CG122600 Rh2DG122500
rosa_wichuraiana Rw2G009230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 463
AccI GTMKAC 2 cut(s) 393, 581
AccII CGCG 2 cut(s) 84, 563
AciI CCGC 8 cut(s) 84, 88, 151, 298, 346, 370, 561, 563
AclWI GGATC 1 cut(s) 71
AcoI YGGCCR 2 cut(s) 85, 367
AcsI RAATTY 1 cut(s) 113
AcuI CTGAAG 1 cut(s) 43
AcvI CACGTG 1 cut(s) 442
AcyI GRCGYC 2 cut(s) 279, 294
AfaI GTAC 5 cut(s) 212, 410, 479, 551, 587
AjiI CACGTC 1 cut(s) 74
AjnI CCWGG 1 cut(s) 339
AluBI AGCT 4 cut(s) 49, 125, 227, 403
AluI AGCT 4 cut(s) 49, 125, 227, 403
Alw21I GWGCWC 4 cut(s) 219, 229, 441, 504
Alw44I GTGCAC 1 cut(s) 215
AlwI GGATC 1 cut(s) 71
AoxI GGCC 4 cut(s) 85, 334, 342, 367
ApaLI GTGCAC 1 cut(s) 215
ApeKI GCWGC 1 cut(s) 28
ApoI RAATTY 1 cut(s) 113
AspLEI GCGC 1 cut(s) 559
AspS9I GGNCC 1 cut(s) 343
AsuC2I CCSGG 2 cut(s) 249, 333
AsuHPI GGTGA 1 cut(s) 373
BaeGI GKGCMC 1 cut(s) 219
BanI GGYRCC 1 cut(s) 463
BanII GRGCYC 1 cut(s) 229
BbrPI CACGTG 1 cut(s) 442
Bbv12I GWGCWC 4 cut(s) 219, 229, 441, 504
BbvCI CCTCAGC 1 cut(s) 404
BbvI GCAGC 1 cut(s) 40
BccI CCATC 2 cut(s) 290, 508
BceAI ACGGC 3 cut(s) 181, 245, 299
BciT130I CCWGG 1 cut(s) 341
BcnI CCSGG 2 cut(s) 249, 333
BisI GCNGC 4 cut(s) 29, 85, 88, 370
BlsI GCNGC 4 cut(s) 30, 86, 89, 371
Bme1390I CCNGG 3 cut(s) 249, 333, 341
BmgBI CACGTC 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 343
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 3 cut(s) 249, 333, 341
BmsI GCATC 4 cut(s) 111, 133, 544, 587
BpmI CTGGAG 2 cut(s) 243, 323
Bpu10I CCTNAGC 1 cut(s) 404
BpuMI CCSGG 2 cut(s) 249, 333
BsaAI YACGTR 3 cut(s) 214, 412, 442
BsaHI GRCGYC 2 cut(s) 279, 294
BsaJI CCNNGG 4 cut(s) 247, 331, 542, 561
Bse1I ACTGG 1 cut(s) 226
BseBI CCWGG 1 cut(s) 341
BseDI CCNNGG 4 cut(s) 247, 331, 542, 561
BseGI GGATG 2 cut(s) 282, 538
BseMII CTCAG 2 cut(s) 270, 395
BseNI ACTGG 1 cut(s) 226
BseRI GAGGAG 3 cut(s) 179, 460, 463
BseSI GKGCMC 1 cut(s) 219
BseX3I CGGCCG 2 cut(s) 85, 367
BseXI GCAGC 1 cut(s) 40
Bsh1236I CGCG 2 cut(s) 84, 563
Bsh1285I CGRYCG 3 cut(s) 88, 291, 370
BshFI GGCC 4 cut(s) 87, 336, 344, 369
BshNI GGYRCC 1 cut(s) 463
BsiEI CGRYCG 3 cut(s) 88, 291, 370
BsiHKAI GWGCWC 4 cut(s) 219, 229, 441, 504
BsiSI CCGG 3 cut(s) 249, 301, 333
BslFI GGGAC 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 84
BsnI GGCC 4 cut(s) 87, 336, 344, 369
Bsp1286I GDGCHC 4 cut(s) 219, 229, 441, 504
Bsp143I GATC 6 cut(s) 63, 183, 266, 288, 481, 535
BspACI CCGC 8 cut(s) 84, 88, 151, 298, 346, 370, 561, 563
BspANI GGCC 4 cut(s) 87, 336, 344, 369
BspCNI CTCAG 2 cut(s) 269, 396
BspFNI CGCG 2 cut(s) 84, 563
BspHI TCATGA 1 cut(s) 484
BspLI GGNNCC 1 cut(s) 465
BspPI GGATC 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 463
BsrI ACTGG 1 cut(s) 226
BssECI CCNNGG 4 cut(s) 247, 331, 542, 561
BssMI GATC 6 cut(s) 63, 183, 266, 288, 481, 535
BssNAI GTATAC 1 cut(s) 394
BssNI GRCGYC 2 cut(s) 279, 294
Bst1107I GTATAC 1 cut(s) 394
Bst2UI CCWGG 1 cut(s) 341
Bst4CI ACNGT 2 cut(s) 94, 506
BstACI GRCGYC 2 cut(s) 279, 294
BstBAI YACGTR 3 cut(s) 214, 412, 442
BstC8I GCNNGC 3 cut(s) 346, 419, 561
BstDEI CTNAG 3 cut(s) 256, 404, 606
BstDSI CCRYGG 1 cut(s) 561
BstF5I GGATG 2 cut(s) 282, 538
BstFNI CGCG 2 cut(s) 84, 563
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 6 cut(s) 66, 186, 269, 291, 484, 538
BstMBI GATC 6 cut(s) 63, 183, 266, 288, 481, 535
BstMCI CGRYCG 3 cut(s) 88, 291, 370
BstMWI GCNNNNNNNGC 2 cut(s) 108, 214
BstNI CCWGG 1 cut(s) 341
BstSCI CCNGG 3 cut(s) 247, 331, 339
BstSLI GKGCMC 1 cut(s) 219
BstUI CGCG 2 cut(s) 84, 563
BstV1I GCAGC 1 cut(s) 40
BstZ17I GTATAC 1 cut(s) 394
BstZI CGGCCG 2 cut(s) 85, 367
BsuRI GGCC 4 cut(s) 87, 336, 344, 369
BtgI CCRYGG 1 cut(s) 561
BtrI CACGTC 1 cut(s) 74
BtsCI GGATG 2 cut(s) 282, 538
BtsIMutI CAGTG 1 cut(s) 219
Cac8I GCNNGC 3 cut(s) 346, 419, 561
CciI TCATGA 1 cut(s) 484
CciNI GCGGCCGC 1 cut(s) 85
CfoI GCGC 1 cut(s) 559
Cfr13I GGNCC 1 cut(s) 343
Cfr42I CCGCGG 1 cut(s) 564
CseI GACGC 2 cut(s) 283, 287
Csp6I GTAC 5 cut(s) 211, 409, 478, 550, 586
CviAII CATG 2 cut(s) 485, 592
CviQI GTAC 5 cut(s) 211, 409, 478, 550, 586
DdeI CTNAG 3 cut(s) 256, 404, 606
DpnI GATC 6 cut(s) 65, 185, 268, 290, 483, 537
DpnII GATC 6 cut(s) 63, 183, 266, 288, 481, 535
EaeI YGGCCR 2 cut(s) 85, 367
EagI CGGCCG 2 cut(s) 85, 367
EciI GGCGGA 1 cut(s) 287
Ecl136II GAGCTC 1 cut(s) 227
EclXI CGGCCG 2 cut(s) 85, 367
Eco24I GRGCYC 1 cut(s) 229
Eco52I CGGCCG 2 cut(s) 85, 367
Eco53kI GAGCTC 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 43
Eco72I CACGTG 1 cut(s) 442
EcoICRI GAGCTC 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 339
EcoT22I ATGCAT 2 cut(s) 6, 602
EcoT38I GRGCYC 1 cut(s) 229
FaeI CATG 2 cut(s) 488, 595
FaiI YATR 4 cut(s) 6, 394, 486, 593
FaqI GGGAC 1 cut(s) 84
FatI CATG 2 cut(s) 484, 591
FauI CCCGC 2 cut(s) 353, 568
FblI GTMKAC 2 cut(s) 393, 581
Fnu4HI GCNGC 4 cut(s) 29, 85, 88, 370
FokI GGATG 2 cut(s) 269, 525
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 4 cut(s) 29, 85, 88, 370
GlaI GCGC 1 cut(s) 558
GluI GCNGC 4 cut(s) 29, 85, 88, 370
GsuI CTGGAG 2 cut(s) 243, 323
HaeIII GGCC 4 cut(s) 87, 336, 344, 369
HapII CCGG 3 cut(s) 249, 301, 333
HgaI GACGC 2 cut(s) 283, 287
HhaI GCGC 1 cut(s) 559
Hin1I GRCGYC 2 cut(s) 279, 294
Hin1II CATG 2 cut(s) 488, 595
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HincII GTYRAC 1 cut(s) 582
HindII GTYRAC 1 cut(s) 582
HinfI GANTC 4 cut(s) 20, 252, 304, 569
HpaII CCGG 3 cut(s) 249, 301, 333
HphI GGTGA 1 cut(s) 373
Hpy166II GTNNAC 5 cut(s) 217, 394, 478, 550, 582
Hpy188I TCNGA 5 cut(s) 19, 25, 266, 510, 543
Hpy188III TCNNGA 6 cut(s) 43, 61, 187, 286, 308, 485
Hpy8I GTNNAC 5 cut(s) 217, 394, 478, 550, 582
Hpy99I CGWCG 3 cut(s) 281, 389, 586
HpyAV CCTTC 2 cut(s) 107, 373
HpyCH4III ACNGT 2 cut(s) 94, 506
HpyCH4IV ACGT 5 cut(s) 73, 213, 411, 441, 584
HpyCH4V TGCA 3 cut(s) 4, 217, 600
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 214
HpyF3I CTNAG 3 cut(s) 256, 404, 606
HpySE526I ACGT 5 cut(s) 73, 213, 411, 441, 584
Hsp92I GRCGYC 2 cut(s) 279, 294
Hsp92II CATG 2 cut(s) 488, 595
HspAI GCGC 1 cut(s) 557
KspI CCGCGG 1 cut(s) 564
Kzo9I GATC 6 cut(s) 63, 183, 266, 288, 481, 535
LmnI GCTCC 4 cut(s) 224, 436, 450, 499
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 4 cut(s) 111, 133, 544, 587
MaeII ACGT 5 cut(s) 73, 213, 411, 441, 584
MaeIII GTNAC 1 cut(s) 74
MalI GATC 6 cut(s) 65, 185, 268, 290, 483, 537
MboI GATC 6 cut(s) 63, 183, 266, 288, 481, 535
MboII GAAGA 1 cut(s) 49
MhlI GDGCHC 4 cut(s) 219, 229, 441, 504
MluCI AATT 2 cut(s) 113, 129
Mph1103I ATGCAT 2 cut(s) 6, 602
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 3 cut(s) 249, 301, 333
MspR9I CCNGG 3 cut(s) 249, 333, 341
MvaI CCWGG 1 cut(s) 341
MvnI CGCG 2 cut(s) 84, 563
MwoI GCNNNNNNNGC 2 cut(s) 108, 214
NciI CCSGG 2 cut(s) 249, 333
NdeII GATC 6 cut(s) 63, 183, 266, 288, 481, 535
NlaIII CATG 2 cut(s) 488, 595
NlaIV GGNNCC 1 cut(s) 465
NmuCI GTSAC 1 cut(s) 74
NotI GCGGCCGC 1 cut(s) 85
NsiI ATGCAT 2 cut(s) 6, 602
PagI TCATGA 1 cut(s) 484
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 578
PfeI GAWTC 4 cut(s) 20, 252, 304, 569
PkrI GCNGC 4 cut(s) 30, 86, 89, 371
Ple19I CGATCG 1 cut(s) 291
PmaCI CACGTG 1 cut(s) 442
PmlI CACGTG 1 cut(s) 442
Ppu21I YACGTR 3 cut(s) 214, 412, 442
Psp124BI GAGCTC 1 cut(s) 229
Psp6I CCWGG 1 cut(s) 339
PspCI CACGTG 1 cut(s) 442
PspGI CCWGG 1 cut(s) 339
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 1 cut(s) 343
PvuI CGATCG 1 cut(s) 291
RsaI GTAC 5 cut(s) 212, 410, 479, 551, 587
RsaNI GTAC 5 cut(s) 211, 409, 478, 550, 586
SacI GAGCTC 1 cut(s) 229
SacII CCGCGG 1 cut(s) 564
SalI GTCGAC 1 cut(s) 580
SatI GCNGC 4 cut(s) 29, 85, 88, 370
Sau3AI GATC 6 cut(s) 63, 183, 266, 288, 481, 535
Sau96I GGNCC 1 cut(s) 343
ScrFI CCNGG 3 cut(s) 249, 333, 341
SduI GDGCHC 4 cut(s) 219, 229, 441, 504
SfaNI GCATC 4 cut(s) 111, 133, 544, 587
Sfr303I CCGCGG 1 cut(s) 564
SgrBI CCGCGG 1 cut(s) 564
Sse9I AATT 2 cut(s) 113, 129
SsiI CCGC 8 cut(s) 84, 88, 151, 298, 346, 370, 561, 563
SspI AATATT 1 cut(s) 174
SstI GAGCTC 1 cut(s) 229
StyD4I CCNGG 3 cut(s) 247, 331, 339
TaaI ACNGT 2 cut(s) 94, 506
TaiI ACGT 5 cut(s) 76, 216, 414, 444, 587
TaqI TCGA 7 cut(s) 42, 51, 127, 186, 309, 423, 581
TasI AATT 2 cut(s) 113, 129
TauI GCSGC 3 cut(s) 87, 90, 372
TfiI GAWTC 4 cut(s) 20, 252, 304, 569
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 1 cut(s) 74
TseI GCWGC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 74
TspGWI ACGGA 2 cut(s) 411, 537
TspRI CASTG 1 cut(s) 226
VneI GTGCAC 1 cut(s) 215
XapI RAATTY 1 cut(s) 113
XmiI GTMKAC 2 cut(s) 393, 581
Zsp2I ATGCAT 2 cut(s) 6, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.