MD02G1122200.v1.1

Ganglioside-induced differentiation-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015291
Physical Location & Seq
Reverse (-)
9953964 .. 9955432
1469 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1122200.v1.1.491

Sequence Viewer

Length: 609 bp
ATGCATACTCAAATCTCCGATTCCGAGCAGCAGGAACTCATCGAAAAGCTCGACATCTTCAAGATCAAAGGACGAGATAAACGCGGCCGCACCGTCCTTCGCATCATCGGCAAATTCTTTCCAGCTCGAATTGTGAGCGTGGATGCTCTGCGGAAGTATTTGGAGGAGAGGATATTTCCAGAGATCGAGAAAAAGCCGTTCGCCGTGCTGTACGTGCACACTGGAGCCCAAAGGTGCGAGAACTTCCCCGGGATCTCAGCCGTCCGATCAATCTACGACGCCGTACCAATCGGCGTCCGCCAGAATCTCGAAGCCGTGTATATCCTCCACCCCGGCCTCCAGGCCCGGCTCTTCCTCGCCACCTTCGGCCGCTTCTTTTTCACCGGCGGGGTGTATGGGAAGCTGAGGTACGTGAGCAGGTTGGATTATCTGTGGGAACATGTGAGGAGGAACGAGATTGAGTTGCCTGATTTCGTGTACGATCATGATGAGGACTTGGAGCACCGTCCGATGATGGACTATGGATTGGAGAGCGATCATCCGAGGGTGTACGATGCACCCGCGGTGGATTCACCCGTGTCGACGTACTCCATGAGATGCATCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2

Protein Analysis

203

Amino Acids

23.54

Weight (kDa)

6.66

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_2 PF13716 29 - 165 3.4e-39 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015291)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35750
fragaria_vesca FvH4_1g10910
malus_domestica MD02G1122200.v1.1 MD15G1236100.v1.1
prunus_persica Prupe.7G176400_v2.0.a1
pyrus_communis pycom02g09510 pycom15g21010
rosa_chinensis RchiOBHm_Chr2g0098171
rosa_laevigata RLG00000016768
rosa_multiflora Rmu_sc0009178.1_g000010
rosa_roxburghii Rroxscaffold_2G00144230
rosa_rugosa Rorug02G0070700
rosa_samantha Rh2BG121000 Rh2CG122600 Rh2DG122500
rosa_wichuraiana Rw2G009230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 408
AccI GTMKAC 1 cut(s) 581
AccII CGCG 2 cut(s) 84, 563
AciI CCGC 8 cut(s) 84, 88, 151, 298, 370, 387, 561, 563
AclWI GGATC 1 cut(s) 260
AcoI YGGCCR 2 cut(s) 85, 367
AcsI RAATTY 1 cut(s) 113
AcyI GRCGYC 2 cut(s) 279, 294
AfaI GTAC 6 cut(s) 212, 285, 410, 479, 551, 587
AflIII ACRYGT 1 cut(s) 439
AgsI TTSAA 1 cut(s) 61
AjnI CCWGG 1 cut(s) 339
AluBI AGCT 3 cut(s) 49, 125, 403
AluI AGCT 3 cut(s) 49, 125, 403
Alw21I GWGCWC 2 cut(s) 219, 504
Alw44I GTGCAC 1 cut(s) 215
AlwI GGATC 1 cut(s) 260
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 4 cut(s) 85, 334, 342, 367
ApaLI GTGCAC 1 cut(s) 215
ApeKI GCWGC 1 cut(s) 28
ApoI RAATTY 1 cut(s) 113
AspS9I GGNCC 1 cut(s) 343
AsuC2I CCSGG 4 cut(s) 249, 250, 333, 346
AsuHPI GGTGA 2 cut(s) 373, 564
AvaI CYCGRG 1 cut(s) 248
BaeGI GKGCMC 1 cut(s) 219
BanII GRGCYC 1 cut(s) 229
BarI GAAGNNNNNNTAC 2 cut(s) 392, 424
Bbv12I GWGCWC 2 cut(s) 219, 504
BbvCI CCTCAGC 1 cut(s) 404
BbvI GCAGC 1 cut(s) 40
BccI CCATC 1 cut(s) 508
BceAI ACGGC 5 cut(s) 181, 188, 245, 266, 299
BciT130I CCWGG 1 cut(s) 341
BcnI CCSGG 4 cut(s) 249, 250, 333, 346
BfaI CTAG 1 cut(s) 607
BfuAI ACCTGC 1 cut(s) 408
BisI GCNGC 4 cut(s) 29, 85, 88, 370
BlsI GCNGC 4 cut(s) 30, 86, 89, 371
Bme1390I CCNGG 5 cut(s) 249, 250, 333, 341, 346
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 343
BmiI GGNNCC 1 cut(s) 226
BmrFI CCNGG 5 cut(s) 249, 250, 333, 341, 346
BmsI GCATC 4 cut(s) 111, 133, 544, 587
BpmI CTGGAG 2 cut(s) 243, 323
Bpu10I CCTNAGC 1 cut(s) 404
BpuMI CCSGG 4 cut(s) 249, 250, 333, 346
BsaAI YACGTR 2 cut(s) 214, 412
BsaHI GRCGYC 2 cut(s) 279, 294
BsaJI CCNNGG 5 cut(s) 247, 248, 331, 542, 561
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 1 cut(s) 226
BseBI CCWGG 1 cut(s) 341
BseDI CCNNGG 5 cut(s) 247, 248, 331, 542, 561
BseGI GGATG 2 cut(s) 148, 538
BseMII CTCAG 2 cut(s) 270, 395
BseNI ACTGG 1 cut(s) 226
BseRI GAGGAG 2 cut(s) 179, 460
BseSI GKGCMC 1 cut(s) 219
BseX3I CGGCCG 2 cut(s) 85, 367
BseXI GCAGC 1 cut(s) 40
Bsh1236I CGCG 2 cut(s) 84, 563
Bsh1285I CGRYCG 2 cut(s) 88, 370
BshFI GGCC 4 cut(s) 87, 336, 344, 369
BsiEI CGRYCG 2 cut(s) 88, 370
BsiHKAI GWGCWC 2 cut(s) 219, 504
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 4 cut(s) 249, 333, 346, 384
BsnI GGCC 4 cut(s) 87, 336, 344, 369
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 3 cut(s) 219, 229, 504
Bsp143I GATC 6 cut(s) 63, 183, 252, 266, 481, 535
BspACI CCGC 8 cut(s) 84, 88, 151, 298, 370, 387, 561, 563
BspANI GGCC 4 cut(s) 87, 336, 344, 369
BspCNI CTCAG 2 cut(s) 269, 396
BspFNI CGCG 2 cut(s) 84, 563
BspHI TCATGA 1 cut(s) 484
BspLI GGNNCC 1 cut(s) 226
BspMI ACCTGC 1 cut(s) 408
BspPI GGATC 1 cut(s) 260
BspQI GCTCTTC 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 383
BsrI ACTGG 1 cut(s) 226
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 5 cut(s) 247, 248, 331, 542, 561
BssMI GATC 6 cut(s) 63, 183, 252, 266, 481, 535
BssNI GRCGYC 2 cut(s) 279, 294
Bst2UI CCWGG 1 cut(s) 341
Bst4CI ACNGT 2 cut(s) 94, 506
Bst6I CTCTTC 1 cut(s) 356
BstACI GRCGYC 2 cut(s) 279, 294
BstBAI YACGTR 2 cut(s) 214, 412
BstDEI CTNAG 2 cut(s) 256, 404
BstDSI CCRYGG 1 cut(s) 561
BstF5I GGATG 2 cut(s) 148, 538
BstFNI CGCG 2 cut(s) 84, 563
BstKTI GATC 6 cut(s) 66, 186, 255, 269, 484, 538
BstMBI GATC 6 cut(s) 63, 183, 252, 266, 481, 535
BstMCI CGRYCG 2 cut(s) 88, 370
BstMWI GCNNNNNNNGC 2 cut(s) 108, 214
BstNI CCWGG 1 cut(s) 341
BstNSI RCATGY 1 cut(s) 443
BstSCI CCNGG 5 cut(s) 247, 248, 331, 339, 344
BstSLI GKGCMC 1 cut(s) 219
BstUI CGCG 2 cut(s) 84, 563
BstV1I GCAGC 1 cut(s) 40
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BstZI CGGCCG 2 cut(s) 85, 367
BsuRI GGCC 4 cut(s) 87, 336, 344, 369
BtgI CCRYGG 1 cut(s) 561
BtsCI GGATG 2 cut(s) 148, 538
BtsIMutI CAGTG 1 cut(s) 219
BveI ACCTGC 1 cut(s) 408
CciI TCATGA 1 cut(s) 484
CciNI GCGGCCGC 1 cut(s) 85
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 343
Cfr42I CCGCGG 1 cut(s) 564
Cfr9I CCCGGG 1 cut(s) 248
CseI GACGC 2 cut(s) 283, 287
Csp6I GTAC 6 cut(s) 211, 284, 409, 478, 550, 586
CviAII CATG 3 cut(s) 440, 485, 592
CviQI GTAC 6 cut(s) 211, 284, 409, 478, 550, 586
DdeI CTNAG 2 cut(s) 256, 404
DpnI GATC 6 cut(s) 65, 185, 254, 268, 483, 537
DpnII GATC 6 cut(s) 63, 183, 252, 266, 481, 535
EaeI YGGCCR 2 cut(s) 85, 367
EagI CGGCCG 2 cut(s) 85, 367
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
EciI GGCGGA 1 cut(s) 287
EclXI CGGCCG 2 cut(s) 85, 367
Eco24I GRGCYC 1 cut(s) 229
Eco52I CGGCCG 2 cut(s) 85, 367
Eco88I CYCGRG 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 339
EcoT22I ATGCAT 2 cut(s) 6, 602
EcoT38I GRGCYC 1 cut(s) 229
FaeI CATG 3 cut(s) 443, 488, 595
FaiI YATR 7 cut(s) 6, 321, 396, 441, 486, 522, 593
FatI CATG 3 cut(s) 439, 484, 591
FauI CCCGC 2 cut(s) 380, 568
FblI GTMKAC 1 cut(s) 581
Fnu4HI GCNGC 4 cut(s) 29, 85, 88, 370
FokI GGATG 2 cut(s) 155, 525
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 4 cut(s) 29, 85, 88, 370
FspBI CTAG 1 cut(s) 607
GluI GCNGC 4 cut(s) 29, 85, 88, 370
GsuI CTGGAG 2 cut(s) 243, 323
HaeIII GGCC 4 cut(s) 87, 336, 344, 369
HapII CCGG 4 cut(s) 249, 333, 346, 384
HgaI GACGC 2 cut(s) 283, 287
Hin1I GRCGYC 2 cut(s) 279, 294
Hin1II CATG 3 cut(s) 443, 488, 595
HincII GTYRAC 1 cut(s) 582
HindII GTYRAC 1 cut(s) 582
HinfI GANTC 3 cut(s) 20, 304, 569
HpaII CCGG 4 cut(s) 249, 333, 346, 384
HphI GGTGA 2 cut(s) 373, 564
Hpy166II GTNNAC 4 cut(s) 217, 478, 550, 582
Hpy188I TCNGA 5 cut(s) 19, 25, 266, 510, 543
Hpy188III TCNNGA 5 cut(s) 61, 179, 187, 308, 485
Hpy8I GTNNAC 4 cut(s) 217, 478, 550, 582
Hpy99I CGWCG 2 cut(s) 281, 586
HpyAV CCTTC 2 cut(s) 107, 373
HpyCH4III ACNGT 2 cut(s) 94, 506
HpyCH4IV ACGT 3 cut(s) 213, 411, 584
HpyCH4V TGCA 4 cut(s) 4, 217, 557, 600
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 214
HpyF3I CTNAG 2 cut(s) 256, 404
HpySE526I ACGT 3 cut(s) 213, 411, 584
Hsp92I GRCGYC 2 cut(s) 279, 294
Hsp92II CATG 3 cut(s) 443, 488, 595
KspI CCGCGG 1 cut(s) 564
Kzo9I GATC 6 cut(s) 63, 183, 252, 266, 481, 535
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 2 cut(s) 224, 499
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 4 cut(s) 111, 133, 544, 587
MaeI CTAG 1 cut(s) 607
MaeII ACGT 3 cut(s) 213, 411, 584
MalI GATC 6 cut(s) 65, 185, 254, 268, 483, 537
MboI GATC 6 cut(s) 63, 183, 252, 266, 481, 535
MboII GAAGA 2 cut(s) 49, 343
MflI RGATCY 1 cut(s) 252
MhlI GDGCHC 3 cut(s) 219, 229, 504
MluCI AATT 2 cut(s) 113, 129
MmeI TCCRAC 1 cut(s) 402
Mph1103I ATGCAT 2 cut(s) 6, 602
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 4 cut(s) 249, 333, 346, 384
MspR9I CCNGG 5 cut(s) 249, 250, 333, 341, 346
MvaI CCWGG 1 cut(s) 341
MvnI CGCG 2 cut(s) 84, 563
MwoI GCNNNNNNNGC 2 cut(s) 108, 214
NciI CCSGG 4 cut(s) 249, 250, 333, 346
NdeII GATC 6 cut(s) 63, 183, 252, 266, 481, 535
NlaIII CATG 3 cut(s) 443, 488, 595
NlaIV GGNNCC 1 cut(s) 226
NotI GCGGCCGC 1 cut(s) 85
NsiI ATGCAT 2 cut(s) 6, 602
NspI RCATGY 1 cut(s) 443
PagI TCATGA 1 cut(s) 484
PciI ACATGT 1 cut(s) 439
PciSI GCTCTTC 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PfeI GAWTC 3 cut(s) 20, 304, 569
PkrI GCNGC 4 cut(s) 30, 86, 89, 371
Ppu21I YACGTR 2 cut(s) 214, 412
PscI ACATGT 1 cut(s) 439
Psp6I CCWGG 1 cut(s) 339
PspGI CCWGG 1 cut(s) 339
PspN4I GGNNCC 1 cut(s) 226
PspPI GGNCC 1 cut(s) 343
PsuI RGATCY 1 cut(s) 252
RsaI GTAC 6 cut(s) 212, 285, 410, 479, 551, 587
RsaNI GTAC 6 cut(s) 211, 284, 409, 478, 550, 586
SacII CCGCGG 1 cut(s) 564
SalI GTCGAC 1 cut(s) 580
SapI GCTCTTC 1 cut(s) 356
SatI GCNGC 4 cut(s) 29, 85, 88, 370
Sau3AI GATC 6 cut(s) 63, 183, 252, 266, 481, 535
Sau96I GGNCC 1 cut(s) 343
ScrFI CCNGG 5 cut(s) 249, 250, 333, 341, 346
SduI GDGCHC 3 cut(s) 219, 229, 504
SfaNI GCATC 4 cut(s) 111, 133, 544, 587
Sfr303I CCGCGG 1 cut(s) 564
SgrAI CRCCGGYG 1 cut(s) 383
SgrBI CCGCGG 1 cut(s) 564
SmaI CCCGGG 1 cut(s) 250
Sse9I AATT 2 cut(s) 113, 129
SsiI CCGC 8 cut(s) 84, 88, 151, 298, 370, 387, 561, 563
SspMI CTAG 1 cut(s) 607
StyD4I CCNGG 5 cut(s) 247, 248, 331, 339, 344
TaaI ACNGT 2 cut(s) 94, 506
TaiI ACGT 3 cut(s) 216, 414, 587
TaqI TCGA 6 cut(s) 42, 51, 127, 186, 309, 581
TasI AATT 2 cut(s) 113, 129
TauI GCSGC 3 cut(s) 87, 90, 372
TfiI GAWTC 3 cut(s) 20, 304, 569
TscAI CASTG 1 cut(s) 226
TseI GCWGC 1 cut(s) 28
TspMI CCCGGG 1 cut(s) 248
TspRI CASTG 1 cut(s) 226
VneI GTGCAC 1 cut(s) 215
XapI RAATTY 1 cut(s) 113
XceI RCATGY 1 cut(s) 443
XmaI CCCGGG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 581
XspI CTAG 1 cut(s) 607
Zsp2I ATGCAT 2 cut(s) 6, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.