Rorug02G0070700

Ganglioside-induced differentiation-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009343
Physical Location & Seq
Forward (+)
5503166 .. 5505578
2413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0070700.1

Sequence Viewer

Length: 1092 bp
ATGGAAGACGGTAAAATCGCAAGCGTGCACTCCACAGTGTTCAAAGAATCAGAGAATTTACAAGGCAAATGCAGCGAAATCAGAGGCTACGATTTCAACGAAGGACTCGATTACTCTCGTCTCTTCGGTTCGATGATCACTACTGGTTTCCAAGCCACCAATCTCGGAGAAGCCATCGAAGTCGTCAACCAAATGCTAGATTGGAGGCTGGCAGATGAGGCTGTAGCTGAGGATTGTGGTGAGGAGGAGAGGGATGGGAAGTTTAGGGAGTCTGTGAAGTGTAAAGTGTTTTTGGGGTTCACTTCGAATTTGGTTTCGTCTGGGCTTAGAGATGTGTTTAGGTACTTGGTGGAGCACCATGTGGTGGATGTGGTGGTCACAACTGCTGGTGGCATTGAGGAGGATCTTATCAAATGCCTTGCGCCGACTTATAAGGGTGATTTCGCTCTGGCAGGGGCGTATTTGAGGTCCAAGGGATTGAATCGTATCGGTAATTTACTGGTTCCTAATGATAACTATTGCAAATTTGAGGATTGGATCATACCGATTTTCGATCAGATGTTGAAGGAACAGAATGAACAGAATGTGCTGTGGACGCCATCTAAATTGATTGCACGCTTGGGGAAGGAAATAAATGATGAAAGATCGTACCTTTACTGGGCATACAAGAACAATGTCCCAGTTTACTGTCCATCCATAACAGATGGCTCATTGGGAGACATGTTGTACTTCCATTCTTTCCGCAGCCCAGGTCTGGTTGTTGACATAGTGCAAGATATTAGGGCCATGAATGGTGAAGCTGTTCATGTTGGTCTGAGGAAGACTGGAATGATCATCCTCGGAGGAGGCCTACCCAAACATCACATTTGCAATGCTAATATGATGCGCAATGGTGCGGATTATGCTGTTTTCATTAATACTGCACAGGAGTATGATGGTAGTGATTCTGGAGCTCGTCCTGATGAGGCTGTGTCATGGGGGAAAATACGGGGTTCTGCTAAAACTGTTAAGGTGCATTGTGATGCAACAATTGCTTTCCCTTTTCTGGTTGCTGAGACATTTGCAAAGAAAGTTAGCAAGACTACAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2

Protein Analysis

363

Amino Acids

40.35

Weight (kDa)

5.39

Isoelectric Point (pI)

30.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DS PF01916 41 - 352 3.6e-123 Deoxyhypusine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 432
Acc16I TGCGCA 1 cut(s) 887
AccB7I CCANNNNNTGG 1 cut(s) 364
AciI CCGC 2 cut(s) 742, 896
AclWI GGATC 2 cut(s) 411, 545
AcsI RAATTY 3 cut(s) 55, 307, 524
AcyI GRCGYC 1 cut(s) 596
AdeI CACNNNGTG 1 cut(s) 361
AfaI GTAC 3 cut(s) 344, 650, 728
AfiI CCNNNNNNNGG 4 cut(s) 364, 658, 754, 1045
AflIII ACRYGT 1 cut(s) 720
AgsI TTSAA 4 cut(s) 43, 97, 481, 565
AjnI CCWGG 1 cut(s) 748
AluBI AGCT 3 cut(s) 227, 800, 953
AluI AGCT 3 cut(s) 227, 800, 953
Alw21I GWGCWC 3 cut(s) 30, 357, 955
Alw26I GTCTC 3 cut(s) 125, 711, 1049
Alw44I GTGCAC 1 cut(s) 26
AlwI GGATC 2 cut(s) 411, 545
AoxI GGCC 2 cut(s) 783, 847
ApaLI GTGCAC 1 cut(s) 26
ApeKI GCWGC 2 cut(s) 72, 744
ApoI RAATTY 3 cut(s) 55, 307, 524
AseI ATTAAT 1 cut(s) 915
Asp700I GAANNNNTTC 1 cut(s) 801
AspLEI GCGC 2 cut(s) 424, 888
AspS9I GGNCC 2 cut(s) 468, 783
AsuHPI GGTGA 3 cut(s) 251, 449, 806
AsuII TTCGAA 1 cut(s) 305
AvaII GGWCC 1 cut(s) 468
BaeGI GKGCMC 1 cut(s) 30
BanII GRGCYC 1 cut(s) 955
BbsI GAAGAC 2 cut(s) 12, 827
Bbv12I GWGCWC 3 cut(s) 30, 357, 955
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 2 cut(s) 84, 756
BccI CCATC 6 cut(s) 182, 248, 607, 698, 700, 929
BciT130I CCWGG 1 cut(s) 750
BclI TGATCA 2 cut(s) 135, 831
BcoDI GTCTC 3 cut(s) 125, 711, 1049
BfaI CTAG 2 cut(s) 197, 1090
BfmI CTRYAG 1 cut(s) 222
BisI GCNGC 2 cut(s) 73, 745
BlsI GCNGC 2 cut(s) 74, 746
Bme1390I CCNGG 1 cut(s) 750
Bme18I GGWCC 1 cut(s) 468
BmgT120I GGNCC 2 cut(s) 468, 783
BmiI GGNNCC 1 cut(s) 504
BmrFI CCNGG 1 cut(s) 750
BmrI ACTGGG 2 cut(s) 667, 674
BmsI GCATC 2 cut(s) 873, 1012
BmuI ACTGGG 2 cut(s) 667, 674
BpiI GAAGAC 2 cut(s) 12, 827
BpmI CTGGAG 1 cut(s) 969
Bpu10I CCTNAGC 1 cut(s) 228
Bpu14I TTCGAA 1 cut(s) 305
BsaHI GRCGYC 1 cut(s) 596
BsaJI CCNNGG 3 cut(s) 471, 748, 838
Bsc4I CCNNNNNNNGG 4 cut(s) 364, 658, 754, 1045
Bse1I ACTGG 5 cut(s) 148, 504, 662, 680, 829
Bse3DI GCAATG 2 cut(s) 877, 895
BseBI CCWGG 1 cut(s) 750
BseDI CCNNGG 3 cut(s) 471, 748, 838
BseGI GGATG 4 cut(s) 259, 373, 692, 834
BseLI CCNNNNNNNGG 4 cut(s) 364, 658, 754, 1045
BseMI GCAATG 2 cut(s) 877, 895
BseMII CTCAG 3 cut(s) 219, 806, 1044
BseNI ACTGG 5 cut(s) 148, 504, 662, 680, 829
BseRI GAGGAG 4 cut(s) 257, 260, 413, 858
BseSI GKGCMC 1 cut(s) 30
BseXI GCAGC 2 cut(s) 84, 756
BsgI GTGCAG 1 cut(s) 906
BshFI GGCC 2 cut(s) 785, 849
BsiHKAI GWGCWC 3 cut(s) 30, 357, 955
BslFI GGGAC 1 cut(s) 662
BslI CCNNNNNNNGG 4 cut(s) 364, 658, 754, 1045
BsmAI GTCTC 3 cut(s) 125, 711, 1049
BsmBI CGTCTC 1 cut(s) 125
BsmFI GGGAC 1 cut(s) 662
BsnI GGCC 2 cut(s) 785, 849
Bsp119I TTCGAA 1 cut(s) 305
Bsp1286I GDGCHC 3 cut(s) 30, 357, 955
Bsp143I GATC 6 cut(s) 135, 403, 537, 553, 644, 831
BspACI CCGC 2 cut(s) 742, 896
BspANI GGCC 2 cut(s) 785, 849
BspCNI CTCAG 3 cut(s) 220, 807, 1045
BspLI GGNNCC 1 cut(s) 504
BspPI GGATC 2 cut(s) 411, 545
BspT104I TTCGAA 1 cut(s) 305
BsrDI GCAATG 2 cut(s) 877, 895
BsrI ACTGG 5 cut(s) 148, 504, 662, 680, 829
BssECI CCNNGG 3 cut(s) 471, 748, 838
BssMI GATC 6 cut(s) 135, 403, 537, 553, 644, 831
BssNI GRCGYC 1 cut(s) 596
BssT1I CCWWGG 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 750
Bst4CI ACNGT 4 cut(s) 11, 37, 689, 1006
Bst6I CTCTTC 1 cut(s) 128
BstACI GRCGYC 1 cut(s) 596
BstAPI GCANNNNNTGC 1 cut(s) 1031
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 4 cut(s) 22, 26, 210, 616
BstDEI CTNAG 4 cut(s) 228, 326, 815, 1053
BstF5I GGATG 4 cut(s) 259, 373, 692, 834
BstHHI GCGC 2 cut(s) 424, 888
BstKTI GATC 6 cut(s) 138, 406, 540, 556, 647, 834
BstMAI GTCTC 3 cut(s) 125, 711, 1049
BstMBI GATC 6 cut(s) 135, 403, 537, 553, 644, 831
BstMWI GCNNNNNNNGC 5 cut(s) 72, 218, 595, 902, 1031
BstNI CCWGG 1 cut(s) 750
BstNSI RCATGY 1 cut(s) 724
BstSCI CCNGG 1 cut(s) 748
BstSFI CTRYAG 1 cut(s) 222
BstSLI GKGCMC 1 cut(s) 30
BstV1I GCAGC 2 cut(s) 84, 756
BstV2I GAAGAC 2 cut(s) 12, 827
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BsuRI GGCC 2 cut(s) 785, 849
BtsCI GGATG 4 cut(s) 259, 373, 692, 834
BtsIMutI CAGTG 1 cut(s) 42
Cac8I GCNNGC 4 cut(s) 22, 26, 210, 616
CfoI GCGC 2 cut(s) 424, 888
Cfr13I GGNCC 2 cut(s) 468, 783
CseI GACGC 1 cut(s) 604
Csp6I GTAC 3 cut(s) 343, 649, 727
CviAII CATG 5 cut(s) 359, 721, 787, 806, 975
CviQI GTAC 3 cut(s) 343, 649, 727
DdeI CTNAG 4 cut(s) 228, 326, 815, 1053
DpnI GATC 6 cut(s) 137, 405, 539, 555, 646, 833
DpnII GATC 6 cut(s) 135, 403, 537, 553, 644, 831
DraIII CACNNNGTG 1 cut(s) 361
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Ecl136II GAGCTC 1 cut(s) 953
Eco130I CCWWGG 1 cut(s) 471
Eco147I AGGCCT 1 cut(s) 849
Eco24I GRGCYC 1 cut(s) 955
Eco47I GGWCC 1 cut(s) 468
Eco53kI GAGCTC 1 cut(s) 953
EcoICRI GAGCTC 1 cut(s) 953
EcoRII CCWGG 1 cut(s) 748
EcoT14I CCWWGG 1 cut(s) 471
EcoT38I GRGCYC 1 cut(s) 955
ErhI CCWWGG 1 cut(s) 471
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 5 cut(s) 362, 724, 790, 809, 978
FaqI GGGAC 1 cut(s) 662
FatI CATG 5 cut(s) 358, 720, 786, 805, 974
FbaI TGATCA 2 cut(s) 135, 831
Fnu4HI GCNGC 2 cut(s) 73, 745
FokI GGATG 4 cut(s) 266, 380, 679, 821
FriOI GRGCYC 1 cut(s) 955
Fsp4HI GCNGC 2 cut(s) 73, 745
FspBI CTAG 2 cut(s) 197, 1090
FspI TGCGCA 1 cut(s) 887
GlaI GCGC 2 cut(s) 423, 887
GluI GCNGC 2 cut(s) 73, 745
GsuI CTGGAG 1 cut(s) 969
HaeIII GGCC 2 cut(s) 785, 849
HgaI GACGC 1 cut(s) 604
HhaI GCGC 2 cut(s) 424, 888
Hin1I GRCGYC 1 cut(s) 596
Hin1II CATG 5 cut(s) 362, 724, 790, 809, 978
Hin6I GCGC 2 cut(s) 422, 886
HinP1I GCGC 2 cut(s) 422, 886
HincII GTYRAC 2 cut(s) 187, 763
HindII GTYRAC 2 cut(s) 187, 763
HinfI GANTC 5 cut(s) 47, 105, 269, 481, 944
HphI GGTGA 3 cut(s) 251, 449, 806
Hpy166II GTNNAC 6 cut(s) 28, 187, 300, 594, 685, 763
Hpy188I TCNGA 6 cut(s) 52, 83, 167, 558, 816, 842
Hpy188III TCNNGA 2 cut(s) 948, 959
Hpy8I GTNNAC 6 cut(s) 28, 187, 300, 594, 685, 763
HpyAV CCTTC 3 cut(s) 95, 559, 619
HpyCH4III ACNGT 4 cut(s) 11, 37, 689, 1006
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 218, 595, 902, 1031
HpyF3I CTNAG 4 cut(s) 228, 326, 815, 1053
Hsp92I GRCGYC 1 cut(s) 596
Hsp92II CATG 5 cut(s) 362, 724, 790, 809, 978
HspAI GCGC 2 cut(s) 422, 886
Ksp22I TGATCA 2 cut(s) 135, 831
Kzo9I GATC 6 cut(s) 135, 403, 537, 553, 644, 831
LmnI GCTCC 2 cut(s) 352, 950
Lsp1109I GCAGC 2 cut(s) 84, 756
LweI GCATC 2 cut(s) 873, 1012
MaeI CTAG 2 cut(s) 197, 1090
MaeIII GTNAC 1 cut(s) 376
MalI GATC 6 cut(s) 137, 405, 539, 555, 646, 833
MboI GATC 6 cut(s) 135, 403, 537, 553, 644, 831
MboII GAAGA 3 cut(s) 17, 115, 832
MfeI CAATTG 1 cut(s) 1029
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 3 cut(s) 30, 357, 955
MluCI AATT 6 cut(s) 55, 307, 493, 524, 605, 1029
MlyI GAGTC 2 cut(s) 99, 278
MroXI GAANNNNTTC 1 cut(s) 801
MseI TTAA 2 cut(s) 915, 1008
MslI CAYNNNNRTG 1 cut(s) 1020
MspR9I CCNGG 1 cut(s) 750
MunI CAATTG 1 cut(s) 1029
MvaI CCWGG 1 cut(s) 750
MwoI GCNNNNNNNGC 5 cut(s) 72, 218, 595, 902, 1031
NdeII GATC 6 cut(s) 135, 403, 537, 553, 644, 831
NlaIII CATG 5 cut(s) 362, 724, 790, 809, 978
NlaIV GGNNCC 1 cut(s) 504
NmuCI GTSAC 1 cut(s) 376
NsbI TGCGCA 1 cut(s) 887
NspI RCATGY 1 cut(s) 724
NspV TTCGAA 1 cut(s) 305
PceI AGGCCT 1 cut(s) 849
PciI ACATGT 1 cut(s) 720
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 105
PdmI GAANNNNTTC 1 cut(s) 801
PfeI GAWTC 3 cut(s) 47, 481, 944
PflMI CCANNNNNTGG 1 cut(s) 364
PkrI GCNGC 2 cut(s) 74, 746
PleI GAGTC 2 cut(s) 99, 277
PpsI GAGTC 2 cut(s) 99, 277
PscI ACATGT 1 cut(s) 720
PshBI ATTAAT 1 cut(s) 915
PsiI TTATAA 1 cut(s) 432
Psp124BI GAGCTC 1 cut(s) 955
Psp6I CCWGG 1 cut(s) 748
PspGI CCWGG 1 cut(s) 748
PspN4I GGNNCC 1 cut(s) 504
PspPI GGNCC 2 cut(s) 468, 783
PsuI RGATCY 1 cut(s) 403
RsaI GTAC 3 cut(s) 344, 650, 728
RsaNI GTAC 3 cut(s) 343, 649, 727
RseI CAYNNNNRTG 1 cut(s) 1020
SacI GAGCTC 1 cut(s) 955
SaqAI TTAA 2 cut(s) 915, 1008
SatI GCNGC 2 cut(s) 73, 745
Sau3AI GATC 6 cut(s) 135, 403, 537, 553, 644, 831
Sau96I GGNCC 2 cut(s) 468, 783
SchI GAGTC 2 cut(s) 99, 278
ScrFI CCNGG 1 cut(s) 750
SduI GDGCHC 3 cut(s) 30, 357, 955
SetI ASST 9 cut(s) 229, 344, 470, 654, 754, 802, 955, 1014, 1091
SfaNI GCATC 2 cut(s) 873, 1012
SfcI CTRYAG 1 cut(s) 222
SfuI TTCGAA 1 cut(s) 305
SinI GGWCC 1 cut(s) 468
SmiMI CAYNNNNRTG 1 cut(s) 1020
Sse9I AATT 6 cut(s) 55, 307, 493, 524, 605, 1029
SseBI AGGCCT 1 cut(s) 849
SsiI CCGC 2 cut(s) 742, 896
SspMI CTAG 2 cut(s) 197, 1090
SstI GAGCTC 1 cut(s) 955
StuI AGGCCT 1 cut(s) 849
StyD4I CCNGG 1 cut(s) 748
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 4 cut(s) 11, 37, 689, 1006
TaqI TCGA 5 cut(s) 108, 131, 177, 305, 552
TasI AATT 6 cut(s) 55, 307, 493, 524, 605, 1029
TatI WGTACW 1 cut(s) 726
TfiI GAWTC 3 cut(s) 47, 481, 944
Tru1I TTAA 2 cut(s) 915, 1008
Tru9I TTAA 2 cut(s) 915, 1008
TscAI CASTG 1 cut(s) 42
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 2 cut(s) 72, 744
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 5 cut(s) 591, 654, 794, 803, 901
TspRI CASTG 1 cut(s) 42
Van91I CCANNNNNTGG 1 cut(s) 364
VneI GTGCAC 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 468
VspI ATTAAT 1 cut(s) 915
XapI RAATTY 3 cut(s) 55, 307, 524
XceI RCATGY 1 cut(s) 724
XmnI GAANNNNTTC 1 cut(s) 801
XspI CTAG 2 cut(s) 197, 1090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.