MD02G1242600.v1.1

UPF0548 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009343
Physical Location & Seq
Forward (+)
29294139 .. 29296153
2015 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1242600.v1.1.491

Sequence Viewer

Length: 843 bp
ATGCCCTGTGTTGAGATACTTGGTCTCTCTGCACCCAAACGCAGAGACCAGTCCCCTTCTCAGTTCAAGCCCAACGCGATAAACCCCCACACCAGTCCGTCTCCGGCGACTTCGACGGCTTCGAAGCAGCTGCGACGGCGGCGCCACCATCTCTTCTCAATTCCACCTCAGATTTCGACTAATCTTTCAGACTTGGACGCAACCGCAACCAAAGTCAGCATGGTTTTCTTGAGCTGGGGCAGGCCAACTCCTCAGGAACAAAAAGCTTGCATTGACAGGTCAGGCAGCTTTAACTATGACCCTAAATTCAAAGGAGCTACTGCTAAGTCCCTGTCTTCCCTCCAAGAAGATAAAGGGCTCTCAAAAGATGGTTTCTTGTTAAACCATTCTCGCAATTTGGTTGGTTCGGGTCTGGACGCTTATGAAAAGGGCAAGATGGCTCTTCAGGACTGGAGGCATTTTGGATTGAATTGGGCATTTGTTGATCCGAAGACGCCAGTTCAAAATGGAGTCAAGTTTTGTGTTTGCGTCAAGGAGTTTCTGCCGTGGGTCATGATGCCTCTCCAGGTTGTATATGTAAATGAAAATAAGAGAAGTAAACAGGCCATGGCATCTTTCCGTTTTGGAGGTGGTACCCTTCAAGGTCATCTGCTGGCTGGGGAAGAACGCTTCTCAATTGAACTGGATGAGAACAACCAAGTGTGGTATGAAATACTTTCCTTCTCGAAACCAGCTCACCTGTTGTCATTCATTGGATACCCATATGTAATGCTTAAGCAGAAGTACTTTGCTCATCAATCTACTAATGCAATGATGAAACATCTGAATGCTTCAAAATCCTAG

Protein Analysis

281

Amino Acids

31.48

Weight (kDa)

9.63

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1990 PF09348 123 - 269 1.5e-36 Domain of unknown function (DUF1990)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 632
AccB1I GGYRCC 2 cut(s) 141, 632
AccII CGCG 1 cut(s) 77
AciI CCGC 2 cut(s) 139, 204
AclWI GGATC 1 cut(s) 479
AcsI RAATTY 1 cut(s) 305
AcuI CTGAAG 1 cut(s) 428
AcyI GRCGYC 2 cut(s) 142, 494
AfaI GTAC 2 cut(s) 634, 785
AflII CTTAAG 1 cut(s) 773
AgsI TTSAA 7 cut(s) 67, 310, 469, 503, 641, 680, 834
AjnI CCWGG 1 cut(s) 564
AluBI AGCT 6 cut(s) 130, 234, 266, 288, 317, 734
AluI AGCT 6 cut(s) 130, 234, 266, 288, 317, 734
Alw26I GTCTC 3 cut(s) 29, 39, 105
AlwI GGATC 1 cut(s) 479
AoxI GGCC 2 cut(s) 242, 603
ApeKI GCWGC 3 cut(s) 127, 130, 285
ApoI RAATTY 1 cut(s) 305
Asp718I GGTACC 1 cut(s) 632
AspLEI GCGC 1 cut(s) 144
AsuHPI GGTGA 1 cut(s) 728
AsuII TTCGAA 1 cut(s) 122
AxyI CCTNAGG 1 cut(s) 252
BanI GGYRCC 2 cut(s) 141, 632
BanII GRGCYC 1 cut(s) 360
BbsI GAAGAC 2 cut(s) 327, 497
BbvI GCAGC 3 cut(s) 117, 139, 297
BccI CCATC 3 cut(s) 156, 362, 430
BceAI ACGGC 3 cut(s) 132, 152, 529
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BciT130I CCWGG 1 cut(s) 566
BciVI GTATCC 1 cut(s) 749
BcoDI GTCTC 3 cut(s) 29, 39, 105
BfaI CTAG 1 cut(s) 841
BfoI RGCGCY 1 cut(s) 145
BfrI CTTAAG 1 cut(s) 773
BfuI GTATCC 1 cut(s) 749
BisI GCNGC 4 cut(s) 128, 131, 140, 286
BlsI GCNGC 4 cut(s) 129, 132, 141, 287
BmcAI AGTACT 1 cut(s) 785
Bme1390I CCNGG 1 cut(s) 566
BmiI GGNNCC 2 cut(s) 143, 634
BmrFI CCNGG 1 cut(s) 566
BmsI GCATC 2 cut(s) 546, 620
BpiI GAAGAC 2 cut(s) 327, 497
BpmI CTGGAG 2 cut(s) 472, 548
Bpu14I TTCGAA 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 250
BsaHI GRCGYC 2 cut(s) 142, 494
BsaI GGTCTC 2 cut(s) 29, 39
BsaJI CCNNGG 2 cut(s) 545, 606
Bse1I ACTGG 5 cut(s) 49, 93, 455, 497, 687
Bse21I CCTNAGG 1 cut(s) 252
Bse3DI GCAATG 1 cut(s) 816
BseBI CCWGG 1 cut(s) 566
BseDI CCNNGG 2 cut(s) 545, 606
BseGI GGATG 1 cut(s) 691
BseMI GCAATG 1 cut(s) 816
BseMII CTCAG 3 cut(s) 74, 182, 266
BseNI ACTGG 5 cut(s) 49, 93, 455, 497, 687
BseRI GAGGAG 1 cut(s) 240
BseXI GCAGC 3 cut(s) 117, 139, 297
BseYI CCCAGC 2 cut(s) 234, 656
BsgI GTGCAG 1 cut(s) 15
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 2 cut(s) 244, 605
BshNI GGYRCC 2 cut(s) 141, 632
BsiSI CCGG 1 cut(s) 104
BslFI GGGAC 2 cut(s) 37, 313
BsmAI GTCTC 3 cut(s) 29, 39, 105
BsmBI CGTCTC 1 cut(s) 105
BsmFI GGGAC 2 cut(s) 37, 313
BsmI GAATGC 1 cut(s) 832
BsnI GGCC 2 cut(s) 244, 605
Bso31I GGTCTC 2 cut(s) 29, 39
Bsp119I TTCGAA 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 360
Bsp143I GATC 1 cut(s) 484
Bsp19I CCATGG 1 cut(s) 606
BspACI CCGC 2 cut(s) 139, 204
BspANI GGCC 2 cut(s) 244, 605
BspCNI CTCAG 3 cut(s) 73, 181, 265
BspFNI CGCG 1 cut(s) 77
BspHI TCATGA 1 cut(s) 552
BspLI GGNNCC 2 cut(s) 143, 634
BspPI GGATC 1 cut(s) 479
BspQI GCTCTTC 1 cut(s) 447
BspT104I TTCGAA 1 cut(s) 122
BspT107I GGYRCC 2 cut(s) 141, 632
BspTI CTTAAG 1 cut(s) 773
BspTNI GGTCTC 2 cut(s) 29, 39
BsrDI GCAATG 1 cut(s) 816
BsrI ACTGG 5 cut(s) 49, 93, 455, 497, 687
BssECI CCNNGG 2 cut(s) 545, 606
BssMI GATC 1 cut(s) 484
BssNI GRCGYC 2 cut(s) 142, 494
BssT1I CCWWGG 1 cut(s) 606
Bst2UI CCWGG 1 cut(s) 566
Bst6I CTCTTC 2 cut(s) 158, 447
BstACI GRCGYC 2 cut(s) 142, 494
BstAFI CTTAAG 1 cut(s) 773
BstBI TTCGAA 1 cut(s) 122
BstC8I GCNNGC 3 cut(s) 242, 268, 654
BstDEI CTNAG 4 cut(s) 60, 168, 252, 324
BstDSI CCRYGG 2 cut(s) 545, 606
BstF5I GGATG 1 cut(s) 691
BstFNI CGCG 1 cut(s) 77
BstH2I RGCGCY 1 cut(s) 145
BstHHI GCGC 1 cut(s) 144
BstKTI GATC 1 cut(s) 487
BstMAI GTCTC 3 cut(s) 29, 39, 105
BstMBI GATC 1 cut(s) 484
BstMWI GCNNNNNNNGC 2 cut(s) 136, 139
BstNI CCWGG 1 cut(s) 566
BstSCI CCNGG 1 cut(s) 564
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 3 cut(s) 117, 139, 297
BstV2I GAAGAC 2 cut(s) 327, 497
Bsu36I CCTNAGG 1 cut(s) 252
BsuI GTATCC 1 cut(s) 749
BsuRI GGCC 2 cut(s) 244, 605
BtgI CCRYGG 2 cut(s) 545, 606
BtsCI GGATG 1 cut(s) 691
Cac8I GCNNGC 3 cut(s) 242, 268, 654
CciI TCATGA 1 cut(s) 552
CfoI GCGC 1 cut(s) 144
CseI GACGC 4 cut(s) 206, 425, 502, 517
Csp6I GTAC 2 cut(s) 633, 784
CviAII CATG 3 cut(s) 220, 553, 607
CviQI GTAC 2 cut(s) 633, 784
DdeI CTNAG 4 cut(s) 60, 168, 252, 324
DinI GGCGCC 1 cut(s) 143
DpnI GATC 1 cut(s) 486
DpnII GATC 1 cut(s) 484
Eam1104I CTCTTC 2 cut(s) 158, 447
EarI CTCTTC 2 cut(s) 158, 447
Eco130I CCWWGG 1 cut(s) 606
Eco24I GRGCYC 1 cut(s) 360
Eco31I GGTCTC 2 cut(s) 29, 39
Eco57I CTGAAG 1 cut(s) 428
Eco81I CCTNAGG 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 564
EcoT14I CCWWGG 1 cut(s) 606
EcoT38I GRGCYC 1 cut(s) 360
EgeI GGCGCC 1 cut(s) 143
EheI GGCGCC 1 cut(s) 143
ErhI CCWWGG 1 cut(s) 606
Esp3I CGTCTC 1 cut(s) 105
FaeI CATG 3 cut(s) 223, 556, 610
FaqI GGGAC 2 cut(s) 37, 313
FatI CATG 3 cut(s) 219, 552, 606
FauNDI CATATG 1 cut(s) 763
Fnu4HI GCNGC 4 cut(s) 128, 131, 140, 286
FokI GGATG 1 cut(s) 698
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 4 cut(s) 128, 131, 140, 286
FspBI CTAG 1 cut(s) 841
GlaI GCGC 1 cut(s) 143
GluI GCNGC 4 cut(s) 128, 131, 140, 286
GsaI CCCAGC 2 cut(s) 238, 660
GsuI CTGGAG 2 cut(s) 472, 548
HaeII RGCGCY 1 cut(s) 145
HaeIII GGCC 2 cut(s) 244, 605
HapII CCGG 1 cut(s) 104
HgaI GACGC 4 cut(s) 206, 425, 502, 517
HhaI GCGC 1 cut(s) 144
Hin1I GRCGYC 2 cut(s) 142, 494
Hin1II CATG 3 cut(s) 223, 556, 610
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 1 cut(s) 510
HpaII CCGG 1 cut(s) 104
HphI GGTGA 1 cut(s) 728
Hpy166II GTNNAC 1 cut(s) 599
Hpy188I TCNGA 4 cut(s) 171, 190, 489, 825
Hpy188III TCNNGA 6 cut(s) 229, 254, 413, 446, 553, 724
Hpy8I GTNNAC 1 cut(s) 599
Hpy99I CGWCG 2 cut(s) 118, 138
HpyAV CCTTC 3 cut(s) 66, 647, 730
HpyCH4V TGCA 3 cut(s) 32, 270, 809
HpyF10VI GCNNNNNNNGC 2 cut(s) 136, 139
HpyF3I CTNAG 4 cut(s) 60, 168, 252, 324
Hsp92I GRCGYC 2 cut(s) 142, 494
Hsp92II CATG 3 cut(s) 223, 556, 610
HspAI GCGC 1 cut(s) 142
KasI GGCGCC 1 cut(s) 141
KpnI GGTACC 1 cut(s) 636
Kzo9I GATC 1 cut(s) 484
LguI GCTCTTC 1 cut(s) 447
LmnI GCTCC 1 cut(s) 314
Lsp1109I GCAGC 3 cut(s) 117, 139, 297
LweI GCATC 2 cut(s) 546, 620
MaeI CTAG 1 cut(s) 841
MalI GATC 1 cut(s) 486
MboI GATC 1 cut(s) 484
MboII GAAGA 6 cut(s) 145, 327, 359, 434, 502, 674
MfeI CAATTG 1 cut(s) 675
MhlI GDGCHC 1 cut(s) 360
MluCI AATT 5 cut(s) 159, 305, 394, 469, 675
Mly113I GGCGCC 1 cut(s) 142
MlyI GAGTC 1 cut(s) 519
MnlI CCTC 6 cut(s) 177, 261, 350, 447, 570, 620
MseI TTAA 3 cut(s) 291, 380, 774
MslI CAYNNNNRTG 1 cut(s) 825
MspA1I CMGCKG 1 cut(s) 130
MspCI CTTAAG 1 cut(s) 773
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 1 cut(s) 566
MunI CAATTG 1 cut(s) 675
Mva1269I GAATGC 1 cut(s) 832
MvaI CCWGG 1 cut(s) 566
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 2 cut(s) 136, 139
NarI GGCGCC 1 cut(s) 142
NcoI CCATGG 1 cut(s) 606
NdeI CATATG 1 cut(s) 763
NdeII GATC 1 cut(s) 484
NlaIII CATG 3 cut(s) 223, 556, 610
NlaIV GGNNCC 2 cut(s) 143, 634
NspV TTCGAA 1 cut(s) 122
PagI TCATGA 1 cut(s) 552
PciSI GCTCTTC 1 cut(s) 447
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PctI GAATGC 1 cut(s) 832
PkrI GCNGC 4 cut(s) 129, 132, 141, 287
PleI GAGTC 1 cut(s) 518
PluTI GGCGCC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 518
Psp6I CCWGG 1 cut(s) 564
PspFI CCCAGC 2 cut(s) 234, 656
PspGI CCWGG 1 cut(s) 564
PspN4I GGNNCC 2 cut(s) 143, 634
PvuII CAGCTG 1 cut(s) 130
RsaI GTAC 2 cut(s) 634, 785
RsaNI GTAC 2 cut(s) 633, 784
RseI CAYNNNNRTG 1 cut(s) 825
SapI GCTCTTC 1 cut(s) 447
SaqAI TTAA 3 cut(s) 291, 380, 774
SatI GCNGC 4 cut(s) 128, 131, 140, 286
Sau3AI GATC 1 cut(s) 484
ScaI AGTACT 1 cut(s) 785
SchI GAGTC 1 cut(s) 519
ScrFI CCNGG 1 cut(s) 566
SduI GDGCHC 1 cut(s) 360
SfaNI GCATC 2 cut(s) 546, 620
SfoI GGCGCC 1 cut(s) 143
SfuI TTCGAA 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 825
SmlI CTYRAG 2 cut(s) 229, 773
SmoI CTYRAG 2 cut(s) 229, 773
Sse9I AATT 5 cut(s) 159, 305, 394, 469, 675
SsiI CCGC 2 cut(s) 139, 204
SspDI GGCGCC 1 cut(s) 141
SspMI CTAG 1 cut(s) 841
StyD4I CCNGG 1 cut(s) 564
StyI CCWWGG 1 cut(s) 606
TaqI TCGA 4 cut(s) 113, 122, 176, 725
TasI AATT 5 cut(s) 159, 305, 394, 469, 675
TatI WGTACW 1 cut(s) 783
TauI GCSGC 1 cut(s) 142
Tru1I TTAA 3 cut(s) 291, 380, 774
Tru9I TTAA 3 cut(s) 291, 380, 774
TseI GCWGC 3 cut(s) 127, 130, 285
TspDTI ATGAA 5 cut(s) 438, 597, 723, 739, 830
TspGWI ACGGA 2 cut(s) 87, 608
Vha464I CTTAAG 1 cut(s) 773
XapI RAATTY 1 cut(s) 305
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XspI CTAG 1 cut(s) 841
ZrmI AGTACT 1 cut(s) 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.