FvH4_1g10900

UPF0548 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009343
Physical Location & Seq
Reverse (-)
5946494 .. 5948539
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g10900.t3

Sequence Viewer

Length: 618 bp
ATGGTTTTCTTGAGCTGGGCTGCCCCAACTCCTAAAGAGCAAAAGGCTTGCATTGACAGGTCAGGTGGCTTTAACTATGAAGCTAAGTTCAGGGGAGCTACTGCCAAGTCCCTATCTTCCCTGCAAGAAGATAAGGGCCTCTCAGAAAATGGTTTCTTATGCAACCATGCCCGCGTTTTGGTCGGTTCGGGTTTGGAGACTTATGAGAAGGGCAAGAGGGCTCTTCAGGACTGGAGGCATTTTGGATTGAATTGGGCGTTTGTTGATCCAAAGACGCCGGTTCAAAATGGAGTGAAGTTTTGTGTTTGTGTCAAGGAGTTTCTGCCATGGGTCATGATGCCTCTTCAGATAGTGTATGTGAATGAAAATAAAAGCAGTAAACAGTCCATGGCGTCTTTTCGTTTTGGGGGTGGTACCCTTCAAGGTCACTTGCTGGCTGGAGAGGAGCGTTTCTCGATTGAACTGGATGAGAATAACCAAGTGTGGTATGAGATACTATCTTTCTCAAAACCAGCTCACGTGTTGTCATTTGTCGGGTACCCATATGTAATGCTCAGGCAAAGGTATTTTGCTCATCATTCTACTAATGCAATCAAGAAACATTTGAATGCCTTGTAA

Protein Analysis

206

Amino Acids

23.24

Weight (kDa)

9.14

Isoelectric Point (pI)

33.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1990 PF09348 61 - 217 1.1e-33 Domain of unknown function (DUF1990)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 413, 537
AccB1I GGYRCC 2 cut(s) 413, 537
AccII CGCG 1 cut(s) 174
AciI CCGC 1 cut(s) 172
AclWI GGATC 1 cut(s) 260
AcuI CTGAAG 2 cut(s) 209, 329
AcvI CACGTG 1 cut(s) 520
AcyI GRCGYC 2 cut(s) 275, 392
AfaI GTAC 2 cut(s) 415, 539
AfiI CCNNNNNNNGG 1 cut(s) 178
AflIII ACRYGT 1 cut(s) 519
AgsI TTSAA 5 cut(s) 250, 284, 422, 461, 607
AluBI AGCT 4 cut(s) 15, 83, 98, 515
AluI AGCT 4 cut(s) 15, 83, 98, 515
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 1 cut(s) 260
AoxI GGCC 1 cut(s) 136
ApeKI GCWGC 1 cut(s) 20
Asp718I GGTACC 2 cut(s) 413, 537
AspS9I GGNCC 1 cut(s) 136
BanI GGYRCC 2 cut(s) 413, 537
BanII GRGCYC 1 cut(s) 223
BbrPI CACGTG 1 cut(s) 520
BbvI GCAGC 1 cut(s) 7
BcoDI GTCTC 1 cut(s) 191
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 2 cut(s) 415, 539
BmsI GCATC 1 cut(s) 327
BplI GAGNNNNNCTC 2 cut(s) 437, 469
BpmI CTGGAG 2 cut(s) 253, 459
Bpu10I CCTNAGC 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 31
BsaAI YACGTR 1 cut(s) 520
BsaHI GRCGYC 2 cut(s) 275, 392
BsaJI CCNNGG 2 cut(s) 326, 387
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 2 cut(s) 236, 468
BseDI CCNNGG 2 cut(s) 326, 387
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMII CTCAG 2 cut(s) 156, 568
BseNI ACTGG 2 cut(s) 236, 468
BseRI GAGGAG 1 cut(s) 458
BseXI GCAGC 1 cut(s) 7
BseYI CCCAGC 1 cut(s) 15
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 1 cut(s) 138
BshNI GGYRCC 2 cut(s) 413, 537
BsiSI CCGG 1 cut(s) 278
BslFI GGGAC 1 cut(s) 94
BslI CCNNNNNNNGG 1 cut(s) 178
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 94
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 1 cut(s) 138
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 1 cut(s) 265
Bsp19I CCATGG 2 cut(s) 326, 387
BspACI CCGC 1 cut(s) 172
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 2 cut(s) 155, 567
BspFNI CGCG 1 cut(s) 174
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 2 cut(s) 415, 539
BspPI GGATC 1 cut(s) 260
BspQI GCTCTTC 1 cut(s) 228
BspT107I GGYRCC 2 cut(s) 413, 537
BsrFI RCCGGY 1 cut(s) 277
BsrI ACTGG 2 cut(s) 236, 468
BssAI RCCGGY 1 cut(s) 277
BssECI CCNNGG 2 cut(s) 326, 387
BssMI GATC 1 cut(s) 265
BssNI GRCGYC 2 cut(s) 275, 392
BssT1I CCWWGG 2 cut(s) 326, 387
Bst4CI ACNGT 1 cut(s) 384
Bst6I CTCTTC 2 cut(s) 228, 348
BstACI GRCGYC 2 cut(s) 275, 392
BstBAI YACGTR 1 cut(s) 520
BstC8I GCNNGC 3 cut(s) 49, 172, 435
BstDEI CTNAG 3 cut(s) 84, 142, 554
BstDSI CCRYGG 2 cut(s) 326, 387
BstF5I GGATG 1 cut(s) 472
BstFNI CGCG 1 cut(s) 174
BstKTI GATC 1 cut(s) 268
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 1 cut(s) 265
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 1 cut(s) 7
BsuRI GGCC 1 cut(s) 138
BtgI CCRYGG 2 cut(s) 326, 387
BtsCI GGATG 1 cut(s) 472
Cac8I GCNNGC 3 cut(s) 49, 172, 435
CciI TCATGA 1 cut(s) 333
Cfr10I RCCGGY 1 cut(s) 277
Cfr13I GGNCC 1 cut(s) 136
CseI GACGC 2 cut(s) 283, 381
Csp6I GTAC 2 cut(s) 414, 538
CviAII CATG 4 cut(s) 167, 327, 334, 388
CviQI GTAC 2 cut(s) 414, 538
DdeI CTNAG 3 cut(s) 84, 142, 554
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
Eam1104I CTCTTC 2 cut(s) 228, 348
EarI CTCTTC 2 cut(s) 228, 348
Eco130I CCWWGG 2 cut(s) 326, 387
Eco24I GRGCYC 1 cut(s) 223
Eco57I CTGAAG 2 cut(s) 209, 329
Eco72I CACGTG 1 cut(s) 520
EcoO109I RGGNCCY 1 cut(s) 136
EcoT14I CCWWGG 2 cut(s) 326, 387
EcoT38I GRGCYC 1 cut(s) 223
ErhI CCWWGG 2 cut(s) 326, 387
FaeI CATG 4 cut(s) 170, 330, 337, 391
FaqI GGGAC 1 cut(s) 94
FatI CATG 4 cut(s) 166, 326, 333, 387
FauI CCCGC 1 cut(s) 179
FauNDI CATATG 1 cut(s) 544
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 1 cut(s) 479
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 1 cut(s) 21
GluI GCNGC 1 cut(s) 21
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 2 cut(s) 253, 459
HaeIII GGCC 1 cut(s) 138
HapII CCGG 1 cut(s) 278
HgaI GACGC 2 cut(s) 283, 381
Hin1I GRCGYC 2 cut(s) 275, 392
Hin1II CATG 4 cut(s) 170, 330, 337, 391
HpaII CCGG 1 cut(s) 278
Hpy166II GTNNAC 1 cut(s) 380
Hpy188I TCNGA 2 cut(s) 145, 348
Hpy188III TCNNGA 5 cut(s) 10, 227, 334, 454, 595
Hpy8I GTNNAC 1 cut(s) 380
HpyAV CCTTC 2 cut(s) 202, 428
HpyCH4III ACNGT 1 cut(s) 384
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 4 cut(s) 51, 124, 162, 590
HpyF3I CTNAG 3 cut(s) 84, 142, 554
HpySE526I ACGT 1 cut(s) 519
Hsp92I GRCGYC 2 cut(s) 275, 392
Hsp92II CATG 4 cut(s) 170, 330, 337, 391
KpnI GGTACC 2 cut(s) 417, 541
Kzo9I GATC 1 cut(s) 265
LguI GCTCTTC 1 cut(s) 228
LmnI GCTCC 2 cut(s) 95, 445
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 1 cut(s) 327
MaeII ACGT 1 cut(s) 519
MaeIII GTNAC 1 cut(s) 425
MalI GATC 1 cut(s) 267
MboI GATC 1 cut(s) 265
MboII GAAGA 4 cut(s) 108, 140, 215, 335
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 1 cut(s) 250
MnlI CCTC 5 cut(s) 149, 210, 228, 351, 436
MseI TTAA 1 cut(s) 72
MslI CAYNNNNRTG 1 cut(s) 606
MspI CCGG 1 cut(s) 278
Mva1269I GAATGC 1 cut(s) 613
MvnI CGCG 1 cut(s) 174
NcoI CCATGG 2 cut(s) 326, 387
NdeI CATATG 1 cut(s) 544
NdeII GATC 1 cut(s) 265
NlaIII CATG 4 cut(s) 170, 330, 337, 391
NlaIV GGNNCC 2 cut(s) 415, 539
NmuCI GTSAC 1 cut(s) 425
PagI TCATGA 1 cut(s) 333
PciSI GCTCTTC 1 cut(s) 228
PctI GAATGC 1 cut(s) 613
PkrI GCNGC 1 cut(s) 22
PmaCI CACGTG 1 cut(s) 520
PmlI CACGTG 1 cut(s) 520
Ppu21I YACGTR 1 cut(s) 520
PspCI CACGTG 1 cut(s) 520
PspFI CCCAGC 1 cut(s) 15
PspN4I GGNNCC 2 cut(s) 415, 539
PspPI GGNCC 1 cut(s) 136
RsaI GTAC 2 cut(s) 415, 539
RsaNI GTAC 2 cut(s) 414, 538
RseI CAYNNNNRTG 1 cut(s) 606
SapI GCTCTTC 1 cut(s) 228
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 1 cut(s) 265
Sau96I GGNCC 1 cut(s) 136
SduI GDGCHC 1 cut(s) 223
SetI ASST 9 cut(s) 17, 62, 67, 85, 100, 427, 517, 522, 566
SfaNI GCATC 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 606
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 1 cut(s) 250
SsiI CCGC 1 cut(s) 172
StyI CCWWGG 2 cut(s) 326, 387
TaaI ACNGT 1 cut(s) 384
TaiI ACGT 1 cut(s) 522
TaqI TCGA 1 cut(s) 455
TasI AATT 1 cut(s) 250
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TseFI GTSAC 1 cut(s) 425
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 1 cut(s) 425
TspDTI ATGAA 2 cut(s) 93, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.