Rh2CG122500

UPF0548 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0009343
Physical Location & Seq
Reverse (-)
10851623 .. 10854722
3100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG122500.1

Sequence Viewer

Length: 522 bp
ATGGTTTTCTTGAGCTGGGCTCCCCCAACTCCTAAGGAACAAAAGGCTTGCATTGACAGGTCAGGTGGCTTTAACTATGAAGCTAAGTTCAGGGGAGCTACTGCTAAGTCCTTATCTTCCCTGCAAGAAGATAAGGGCCTCTCAGAAAATGGTTTCTTATGCAACCATGCCCGCGTTTTGGTCGGTTCAGGTTTGGAGACTTATGAGAAGGGCAAGAGGGCTCTTCAGGATTGGAGGCATTTTGGATTGAATTGGGCGTTTGTTGATCCGAAGACGCCAGTTCAAAATGGAGTGAAGTTTTGTGTTTGTGTCAAGGAGTTTCTGCCATGGGTCATGATGCCTCTTCAGATAGTGTATGTGAATGAGAATAGAAGTAGTAAAGAGTCCATGGCGTCTTTTCGTTTTGGGGGTGGTACTCTTCAAGGTCACTTGCTGGTTAGTTCTTTTTCAAATGTTAAATATATTCATTGTAAAGCCCGAATCTATTTTACTGCATGGTTTTTGGTTCCGAAGCAAACATGA

Protein Analysis

173

Amino Acids

19.57

Weight (kDa)

9.46

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1990 PF09348 47 - 145 8.9e-16 Domain of unknown function (DUF1990)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 174
AciI CCGC 1 cut(s) 172
AclWI GGATC 1 cut(s) 260
AcuI CTGAAG 2 cut(s) 209, 329
AcyI GRCGYC 2 cut(s) 275, 392
AfaI GTAC 1 cut(s) 415
AfiI CCNNNNNNNGG 1 cut(s) 178
AgsI TTSAA 4 cut(s) 250, 284, 422, 450
AluBI AGCT 3 cut(s) 15, 83, 98
AluI AGCT 3 cut(s) 15, 83, 98
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 1 cut(s) 260
AoxI GGCC 1 cut(s) 136
AspS9I GGNCC 1 cut(s) 136
AxyI CCTNAGG 1 cut(s) 33
BanII GRGCYC 2 cut(s) 22, 223
BbsI GAAGAC 1 cut(s) 278
BcoDI GTCTC 1 cut(s) 191
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 2 cut(s) 21, 507
BmsI GCATC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 278
BplI GAGNNNNNCTC 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 31
BsaHI GRCGYC 2 cut(s) 275, 392
BsaJI CCNNGG 2 cut(s) 326, 387
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse1I ACTGG 1 cut(s) 278
Bse21I CCTNAGG 1 cut(s) 33
BseDI CCNNGG 2 cut(s) 326, 387
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 278
BseYI CCCAGC 1 cut(s) 15
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 178
BsmAI GTCTC 1 cut(s) 191
BsnI GGCC 1 cut(s) 138
Bsp1286I GDGCHC 2 cut(s) 22, 223
Bsp143I GATC 1 cut(s) 265
Bsp19I CCATGG 2 cut(s) 326, 387
BspACI CCGC 1 cut(s) 172
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 1 cut(s) 155
BspFNI CGCG 1 cut(s) 174
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 2 cut(s) 21, 507
BspPI GGATC 1 cut(s) 260
BspQI GCTCTTC 1 cut(s) 228
BsrI ACTGG 1 cut(s) 278
BssECI CCNNGG 2 cut(s) 326, 387
BssMI GATC 1 cut(s) 265
BssNI GRCGYC 2 cut(s) 275, 392
BssT1I CCWWGG 2 cut(s) 326, 387
Bst6I CTCTTC 3 cut(s) 228, 348, 423
BstACI GRCGYC 2 cut(s) 275, 392
BstC8I GCNNGC 2 cut(s) 49, 172
BstDEI CTNAG 4 cut(s) 33, 84, 105, 142
BstDSI CCRYGG 2 cut(s) 326, 387
BstFNI CGCG 1 cut(s) 174
BstKTI GATC 1 cut(s) 268
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 1 cut(s) 265
BstUI CGCG 1 cut(s) 174
BstV2I GAAGAC 1 cut(s) 278
Bsu36I CCTNAGG 1 cut(s) 33
BsuRI GGCC 1 cut(s) 138
BtgI CCRYGG 2 cut(s) 326, 387
Cac8I GCNNGC 2 cut(s) 49, 172
CciI TCATGA 1 cut(s) 333
Cfr13I GGNCC 1 cut(s) 136
CseI GACGC 2 cut(s) 283, 381
Csp6I GTAC 1 cut(s) 414
CviAII CATG 6 cut(s) 167, 327, 334, 388, 495, 519
CviJI RGCY 9 cut(s) 15, 20, 47, 69, 83, 98, 138, 221, 476
CviKI_1 RGCY 9 cut(s) 15, 20, 47, 69, 83, 98, 138, 221, 476
CviQI GTAC 1 cut(s) 414
DdeI CTNAG 4 cut(s) 33, 84, 105, 142
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
Eam1104I CTCTTC 3 cut(s) 228, 348, 423
EarI CTCTTC 3 cut(s) 228, 348, 423
Eco130I CCWWGG 2 cut(s) 326, 387
Eco24I GRGCYC 2 cut(s) 22, 223
Eco57I CTGAAG 2 cut(s) 209, 329
Eco81I CCTNAGG 1 cut(s) 33
EcoO109I RGGNCCY 1 cut(s) 136
EcoT14I CCWWGG 2 cut(s) 326, 387
EcoT38I GRGCYC 2 cut(s) 22, 223
ErhI CCWWGG 2 cut(s) 326, 387
FaeI CATG 6 cut(s) 170, 330, 337, 391, 498, 522
FatI CATG 6 cut(s) 166, 326, 333, 387, 494, 518
FauI CCCGC 1 cut(s) 179
FriOI GRGCYC 2 cut(s) 22, 223
GsaI CCCAGC 1 cut(s) 19
HaeIII GGCC 1 cut(s) 138
HgaI GACGC 2 cut(s) 283, 381
Hin1I GRCGYC 2 cut(s) 275, 392
Hin1II CATG 6 cut(s) 170, 330, 337, 391, 498, 522
HinfI GANTC 2 cut(s) 383, 480
Hpy188I TCNGA 4 cut(s) 145, 270, 348, 510
Hpy188III TCNNGA 3 cut(s) 10, 227, 334
HpyAV CCTTC 1 cut(s) 202
HpyCH4V TGCA 4 cut(s) 51, 124, 162, 494
HpyF3I CTNAG 4 cut(s) 33, 84, 105, 142
Hsp92I GRCGYC 2 cut(s) 275, 392
Hsp92II CATG 6 cut(s) 170, 330, 337, 391, 498, 522
Kzo9I GATC 1 cut(s) 265
LguI GCTCTTC 1 cut(s) 228
LmnI GCTCC 2 cut(s) 25, 95
LpnPI CCDG 8 cut(s) 43, 48, 76, 134, 174, 212, 291, 419
LweI GCATC 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 425
MalI GATC 1 cut(s) 267
MboI GATC 1 cut(s) 265
MboII GAAGA 6 cut(s) 108, 140, 215, 283, 335, 410
MhlI GDGCHC 2 cut(s) 22, 223
MluCI AATT 1 cut(s) 250
MlyI GAGTC 1 cut(s) 392
MnlI CCTC 4 cut(s) 149, 210, 228, 351
MseI TTAA 2 cut(s) 72, 456
MvnI CGCG 1 cut(s) 174
NcoI CCATGG 2 cut(s) 326, 387
NdeII GATC 1 cut(s) 265
NlaIII CATG 6 cut(s) 170, 330, 337, 391, 498, 522
NlaIV GGNNCC 2 cut(s) 21, 507
NmuCI GTSAC 1 cut(s) 425
PagI TCATGA 1 cut(s) 333
PciSI GCTCTTC 1 cut(s) 228
PfeI GAWTC 1 cut(s) 480
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
PspFI CCCAGC 1 cut(s) 15
PspN4I GGNNCC 2 cut(s) 21, 507
PspPI GGNCC 1 cut(s) 136
RsaI GTAC 1 cut(s) 415
RsaNI GTAC 1 cut(s) 414
SapI GCTCTTC 1 cut(s) 228
SaqAI TTAA 2 cut(s) 72, 456
Sau3AI GATC 1 cut(s) 265
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 1 cut(s) 392
SduI GDGCHC 2 cut(s) 22, 223
SetI ASST 7 cut(s) 17, 62, 67, 85, 100, 193, 427
SfaNI GCATC 1 cut(s) 327
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 1 cut(s) 250
SsiI CCGC 1 cut(s) 172
StyI CCWWGG 2 cut(s) 326, 387
TasI AATT 1 cut(s) 250
TfiI GAWTC 1 cut(s) 480
Tru1I TTAA 2 cut(s) 72, 456
Tru9I TTAA 2 cut(s) 72, 456
TseFI GTSAC 1 cut(s) 425
Tsp45I GTSAC 1 cut(s) 425
TspDTI ATGAA 2 cut(s) 93, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.