RLG00000016767

UPF0548 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009343
Physical Location & Seq
Reverse (-)
9855825 .. 9857692
1868 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016767

Sequence Viewer

Length: 672 bp
ATGGTTTTCTTGAGCTGGGCTCCCCCAACTCCTAAGGAACAAAAGGCTTGCATTGACAGGTCAGGTGGCTTTAACTATGAAGCTAAGTTCAGGGGAGCTACTGCTAAGTCCTTATCTTCCCTGCAAGAAGATAAGGGCCTCTCAGAAAATGGTTTCTTATGCAACCATGCCCGTGTTTTGGTTGGTTCGGGTTTGGAGACTTATGAGAAGGGCAAGAGGGCTCTTCAGGATTGGAGGCATTTTGGATTGAATTGGGCATTTGTTGATCCGAAGACGCCGGTTCAAAATGGAGTGAAGTTTTGTGTTTGTGTCAAGGAGTTTCTGCCATGGGTCATGATGCCTCTTCAGATAGTGTATGTGAATGAGAATAGAAGTAGTAAAGAGTCCATGGCGTCTTTTCGTTTTGGGGGTGGTACTCTTCAAGGTCACTTGCTGGCTGGGGAGGAACGTTTCTCAATTGAACTGGATGAGTACAACCAAGTGTGGTATGAGATACTTTCCTTCTCAAAACCAGCTCACCTATTGTCATTTGTTGGGTACCCATATGTAATGCTCAGGCAAAGAAGCATAGCGTATCACCTCCTACTTCCTGCCAATAACAATGACCCCTCCCTAAGGGGTTGGTTGGTTAAGCAAGTCATAGATGAAGTGGGCAAGATCGTTAAGATGTAG

Protein Analysis

224

Amino Acids

25.33

Weight (kDa)

8.82

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1990 PF09348 48 - 194 5.8e-36 Domain of unknown function (DUF1990)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 537
AccB1I GGYRCC 1 cut(s) 537
AclI AACGTT 1 cut(s) 448
AclWI GGATC 1 cut(s) 260
AcuI CTGAAG 2 cut(s) 209, 329
AcyI GRCGYC 2 cut(s) 275, 392
AfaI GTAC 3 cut(s) 415, 473, 539
AfiI CCNNNNNNNGG 2 cut(s) 178, 615
AgsI TTSAA 4 cut(s) 250, 284, 422, 461
AluBI AGCT 4 cut(s) 15, 83, 98, 515
AluI AGCT 4 cut(s) 15, 83, 98, 515
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 1 cut(s) 260
AoxI GGCC 1 cut(s) 136
Asp718I GGTACC 1 cut(s) 537
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 509, 569
AxyI CCTNAGG 2 cut(s) 33, 614
BanI GGYRCC 1 cut(s) 537
BanII GRGCYC 2 cut(s) 22, 223
BbsI GAAGAC 1 cut(s) 278
BcoDI GTCTC 1 cut(s) 191
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 2 cut(s) 21, 539
BmsI GCATC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 278
BplI GAGNNNNNCTC 1 cut(s) 36
Bpu10I CCTNAGC 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 31
BsaHI GRCGYC 2 cut(s) 275, 392
BsaJI CCNNGG 2 cut(s) 326, 387
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 615
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 1 cut(s) 468
Bse21I CCTNAGG 2 cut(s) 33, 614
BseDI CCNNGG 2 cut(s) 326, 387
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 2 cut(s) 178, 615
BseMII CTCAG 2 cut(s) 156, 568
BseNI ACTGG 1 cut(s) 468
BseYI CCCAGC 2 cut(s) 15, 437
BshFI GGCC 1 cut(s) 138
BshNI GGYRCC 1 cut(s) 537
BsiSI CCGG 1 cut(s) 278
BslI CCNNNNNNNGG 2 cut(s) 178, 615
BsmAI GTCTC 1 cut(s) 191
BsnI GGCC 1 cut(s) 138
Bsp1286I GDGCHC 2 cut(s) 22, 223
Bsp143I GATC 2 cut(s) 265, 657
Bsp19I CCATGG 2 cut(s) 326, 387
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 2 cut(s) 155, 567
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 2 cut(s) 21, 539
BspPI GGATC 1 cut(s) 260
BspQI GCTCTTC 1 cut(s) 228
BspT107I GGYRCC 1 cut(s) 537
BsrFI RCCGGY 1 cut(s) 277
BsrI ACTGG 1 cut(s) 468
BssAI RCCGGY 1 cut(s) 277
BssECI CCNNGG 2 cut(s) 326, 387
BssMI GATC 2 cut(s) 265, 657
BssNI GRCGYC 2 cut(s) 275, 392
BssT1I CCWWGG 2 cut(s) 326, 387
Bst6I CTCTTC 3 cut(s) 228, 348, 423
BstACI GRCGYC 2 cut(s) 275, 392
BstC8I GCNNGC 2 cut(s) 49, 435
BstDEI CTNAG 6 cut(s) 33, 84, 105, 142, 554, 614
BstDSI CCRYGG 2 cut(s) 326, 387
BstENI CCTNNNNNAGG 1 cut(s) 613
BstF5I GGATG 1 cut(s) 472
BstKTI GATC 2 cut(s) 268, 660
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 2 cut(s) 265, 657
BstV2I GAAGAC 1 cut(s) 278
Bsu36I CCTNAGG 2 cut(s) 33, 614
BsuRI GGCC 1 cut(s) 138
BtgI CCRYGG 2 cut(s) 326, 387
BtsCI GGATG 1 cut(s) 472
Cac8I GCNNGC 2 cut(s) 49, 435
CciI TCATGA 1 cut(s) 333
Cfr10I RCCGGY 1 cut(s) 277
Cfr13I GGNCC 1 cut(s) 136
CseI GACGC 2 cut(s) 283, 381
Csp6I GTAC 3 cut(s) 414, 472, 538
CviAII CATG 4 cut(s) 167, 327, 334, 388
CviQI GTAC 3 cut(s) 414, 472, 538
DdeI CTNAG 6 cut(s) 33, 84, 105, 142, 554, 614
DpnI GATC 2 cut(s) 267, 659
DpnII GATC 2 cut(s) 265, 657
Eam1104I CTCTTC 3 cut(s) 228, 348, 423
EarI CTCTTC 3 cut(s) 228, 348, 423
Eco130I CCWWGG 2 cut(s) 326, 387
Eco24I GRGCYC 2 cut(s) 22, 223
Eco57I CTGAAG 2 cut(s) 209, 329
Eco81I CCTNAGG 2 cut(s) 33, 614
EcoNI CCTNNNNNAGG 1 cut(s) 613
EcoO109I RGGNCCY 1 cut(s) 136
EcoT14I CCWWGG 2 cut(s) 326, 387
EcoT38I GRGCYC 2 cut(s) 22, 223
ErhI CCWWGG 2 cut(s) 326, 387
FaeI CATG 4 cut(s) 170, 330, 337, 391
FatI CATG 4 cut(s) 166, 326, 333, 387
FauNDI CATATG 1 cut(s) 544
FokI GGATG 1 cut(s) 479
FriOI GRGCYC 2 cut(s) 22, 223
GsaI CCCAGC 2 cut(s) 19, 441
HaeIII GGCC 1 cut(s) 138
HapII CCGG 1 cut(s) 278
HgaI GACGC 2 cut(s) 283, 381
Hin1I GRCGYC 2 cut(s) 275, 392
Hin1II CATG 4 cut(s) 170, 330, 337, 391
HinfI GANTC 1 cut(s) 383
HpaII CCGG 1 cut(s) 278
HphI GGTGA 2 cut(s) 509, 569
Hpy188I TCNGA 3 cut(s) 145, 270, 348
Hpy188III TCNNGA 3 cut(s) 10, 227, 334
HpyAV CCTTC 2 cut(s) 202, 511
HpyCH4IV ACGT 1 cut(s) 448
HpyCH4V TGCA 3 cut(s) 51, 124, 162
HpyF3I CTNAG 6 cut(s) 33, 84, 105, 142, 554, 614
HpySE526I ACGT 1 cut(s) 448
Hsp92I GRCGYC 2 cut(s) 275, 392
Hsp92II CATG 4 cut(s) 170, 330, 337, 391
KpnI GGTACC 1 cut(s) 541
Kzo9I GATC 2 cut(s) 265, 657
LguI GCTCTTC 1 cut(s) 228
LmnI GCTCC 2 cut(s) 25, 95
LweI GCATC 1 cut(s) 327
MaeII ACGT 1 cut(s) 448
MaeIII GTNAC 1 cut(s) 425
MalI GATC 2 cut(s) 267, 659
MboI GATC 2 cut(s) 265, 657
MboII GAAGA 6 cut(s) 108, 140, 215, 283, 335, 410
MfeI CAATTG 1 cut(s) 456
MhlI GDGCHC 2 cut(s) 22, 223
MluCI AATT 2 cut(s) 250, 456
MlyI GAGTC 1 cut(s) 392
MnlI CCTC 7 cut(s) 149, 210, 228, 351, 436, 590, 619
MseI TTAA 3 cut(s) 72, 630, 663
MslI CAYNNNNRTG 1 cut(s) 171
MspI CCGG 1 cut(s) 278
MunI CAATTG 1 cut(s) 456
NcoI CCATGG 2 cut(s) 326, 387
NdeI CATATG 1 cut(s) 544
NdeII GATC 2 cut(s) 265, 657
NlaIII CATG 4 cut(s) 170, 330, 337, 391
NlaIV GGNNCC 2 cut(s) 21, 539
NmuCI GTSAC 1 cut(s) 425
PagI TCATGA 1 cut(s) 333
PciSI GCTCTTC 1 cut(s) 228
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
Psp1406I AACGTT 1 cut(s) 448
PspFI CCCAGC 2 cut(s) 15, 437
PspN4I GGNNCC 2 cut(s) 21, 539
PspPI GGNCC 1 cut(s) 136
RsaI GTAC 3 cut(s) 415, 473, 539
RsaNI GTAC 3 cut(s) 414, 472, 538
RseI CAYNNNNRTG 1 cut(s) 171
SapI GCTCTTC 1 cut(s) 228
SaqAI TTAA 3 cut(s) 72, 630, 663
Sau3AI GATC 2 cut(s) 265, 657
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 1 cut(s) 392
SduI GDGCHC 2 cut(s) 22, 223
SfaNI GCATC 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 171
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 2 cut(s) 250, 456
StyI CCWWGG 2 cut(s) 326, 387
TaiI ACGT 1 cut(s) 451
TasI AATT 2 cut(s) 250, 456
TatI WGTACW 1 cut(s) 471
Tru1I TTAA 3 cut(s) 72, 630, 663
Tru9I TTAA 3 cut(s) 72, 630, 663
TseFI GTSAC 1 cut(s) 425
Tsp45I GTSAC 1 cut(s) 425
TspDTI ATGAA 2 cut(s) 93, 660
XagI CCTNNNNNAGG 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.