AT5G01230

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015906
Physical Location & Seq
Reverse (-)
91838 .. 95701
3864 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01230.2

Sequence Viewer

Length: 930 bp
ATGGGAAAAGCTTCTCGAGACAAAAGGGATATCTATTATCGTAAAGCTAAAGAAGAAGGATGGCGTGCAAGAAGTGCTTTTAAGCTTCTTCAGATTGATGAAGAATTTAATATTTTTGAAGGTGTGAAGAGGGTCGTAGATTTATGTGCTGCACCTGGTAGCTGGAGTCAGGTCTTGAGTCGTCAATTATATCTTCCTGCAAAGTCCTCAGCTGAGTCAAAAGATGGGGATCTTCCTCTTATAGTAGCCATCGATTTGCAGCCTATGGCTCCAATTGAAGGTGTCATCCAGGTTCAAGGGGACATAACTAATGCTCGGACTGCTGAAGTGGTCATTAGACACTTTGACGGCTGCAAAGCGGACCTGGTTGTCTGTGATGGTGCTCCAGATGTTACTGGTTTGCATGACATGGATGAATTTGTCCAGTCCCAACTCATACTAGCAGGATTAACGATTGTAACTCATATTCTTAAAGAAGGTGGAAAATTTATTGCAAAGATATTCCGTGGAAAAGATACAAGTCTCTTGTACTGTCAACTGAAATTGTTTTTCCCAACCGTAACTTTTGCGAAACCGAAAAGCAGCCGCAATTCAAGTATAGAGGCTTTTGCAGTCTGCGAGAATTACTCTCCGCCAGAAGGATTTAACCCGAGAGATCTGCATCGTCTCTTGGAGAAAGTCGGAAGCCCTTCAGGTGGAAGCGATCTCGATTGCAGTAGTGGTTGGCTTGAAGGACCCAACAAAGTTTATATACCATTCTTGGCATGTGGTGACTTAACCGGTTATGACTCAGACCGGTCTTACCCACTCCCTAGAGAAGCAGATGGATCATCATACCAGAGTTTGGACCCGATTCAACCTCCGATCGCACCGCCTTATAAACGAGCTCTTGAGCTCAAGAAAGCTTCAGCACAAAGCTTCAACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

309

Amino Acids

33.99

Weight (kDa)

5.9

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 21 - 57 1.6e-13 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015906)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01230
fragaria_vesca FvH4_6g11910 FvH4_6g11910
rosa_chinensis RchiOBHm_Chr3g0463651 RchiOBHm_Chr5g0026681
rosa_laevigata RLG00000032946
rosa_multiflora Rmu_sc0003267.1_g000029
rosa_roxburghii Rroxscaffold_1G00052780 Rroxscaffold_6G00416380
rosa_rugosa Rorug03G0066800.1 Rorug05G0094700
rosa_samantha Rh3DG129100 Rh5BG184700 Rh5CG204600
rosa_wichuraiana Rw3G010470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 879
AasI GACNNNNNNGTC 1 cut(s) 368
AccB7I CCANNNNNTGG 1 cut(s) 844
AciI CCGC 4 cut(s) 359, 586, 632, 872
AclWI GGATC 2 cut(s) 237, 835
AcsI RAATTY 3 cut(s) 104, 416, 485
AcuI CTGAAG 4 cut(s) 74, 345, 675, 891
AfaI GTAC 1 cut(s) 530
AfiI CCNNNNNNNGG 4 cut(s) 278, 638, 695, 844
AgeI ACCGGT 2 cut(s) 779, 795
AgsI TTSAA 7 cut(s) 119, 278, 296, 594, 731, 857, 922
AjnI CCWGG 3 cut(s) 154, 288, 363
AluBI AGCT 9 cut(s) 11, 47, 85, 162, 212, 887, 895, 905, 918
AluI AGCT 9 cut(s) 11, 47, 85, 162, 212, 887, 895, 905, 918
Alw21I GWGCWC 3 cut(s) 385, 889, 897
Alw26I GTCTC 3 cut(s) 12, 527, 671
AlwI GGATC 2 cut(s) 237, 835
Ama87I CYCGRG 2 cut(s) 15, 649
ApeKI GCWGC 4 cut(s) 149, 259, 351, 582
ApoI RAATTY 3 cut(s) 104, 416, 485
AsiGI ACCGGT 2 cut(s) 779, 795
Asp700I GAANNNNTTC 2 cut(s) 10, 688
AspS9I GGNCC 3 cut(s) 361, 734, 847
AsuHPI GGTGA 1 cut(s) 782
AvaI CYCGRG 2 cut(s) 15, 649
AvaII GGWCC 3 cut(s) 361, 734, 847
BanII GRGCYC 2 cut(s) 889, 897
Bbv12I GWGCWC 3 cut(s) 385, 889, 897
BbvCI CCTCAGC 1 cut(s) 208
BbvI GCAGC 4 cut(s) 136, 271, 338, 594
BccI CCATC 5 cut(s) 54, 218, 257, 371, 818
BceAI ACGGC 1 cut(s) 364
BcgI CGANNNNNNTGC 2 cut(s) 640, 674
BciT130I CCWGG 3 cut(s) 156, 290, 365
BcoDI GTCTC 3 cut(s) 12, 527, 671
BfaI CTAG 2 cut(s) 440, 813
BglII AGATCT 1 cut(s) 655
BisI GCNGC 5 cut(s) 150, 260, 352, 583, 586
BlsI GCNGC 5 cut(s) 151, 261, 353, 584, 587
Bme1390I CCNGG 3 cut(s) 156, 290, 365
Bme18I GGWCC 3 cut(s) 361, 734, 847
BmeT110I CYCGRG 2 cut(s) 15, 649
BmgT120I GGNCC 3 cut(s) 361, 734, 847
BmiI GGNNCC 3 cut(s) 270, 736, 849
BmrFI CCNGG 3 cut(s) 156, 290, 365
BmsI GCATC 1 cut(s) 670
BplI GAGNNNNNCTC 2 cut(s) 611, 643
BpmI CTGGAG 2 cut(s) 184, 369
Bpu10I CCTNAGC 1 cut(s) 208
BpuEI CTTGAG 3 cut(s) 196, 881, 911
Bsa29I ATCGAT 1 cut(s) 252
BsaBI GATNNNNATC 2 cut(s) 228, 660
BsaJI CCNNGG 1 cut(s) 505
BsaWI WCCGGW 2 cut(s) 779, 795
Bsc4I CCNNNNNNNGG 4 cut(s) 278, 638, 695, 844
Bse118I RCCGGY 2 cut(s) 779, 795
Bse1I ACTGG 2 cut(s) 400, 424
Bse8I GATNNNNATC 2 cut(s) 228, 660
BseBI CCWGG 3 cut(s) 156, 290, 365
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 1 cut(s) 505
BseGI GGATG 3 cut(s) 65, 285, 418
BseJI GATNNNNATC 2 cut(s) 228, 660
BseLI CCNNNNNNNGG 4 cut(s) 278, 638, 695, 844
BseMII CTCAG 3 cut(s) 204, 222, 804
BseNI ACTGG 2 cut(s) 400, 424
BseXI GCAGC 4 cut(s) 136, 271, 338, 594
BsgI GTGCAG 1 cut(s) 135
Bsh1285I CGRYCG 1 cut(s) 867
BshTI ACCGGT 2 cut(s) 779, 795
BshVI ATCGAT 1 cut(s) 252
BsiEI CGRYCG 1 cut(s) 867
BsiHKAI GWGCWC 3 cut(s) 385, 889, 897
BsiHKCI CYCGRG 2 cut(s) 15, 649
BsiSI CCGG 2 cut(s) 780, 796
BslFI GGGAC 2 cut(s) 314, 412
BslI CCNNNNNNNGG 4 cut(s) 278, 638, 695, 844
BsmAI GTCTC 3 cut(s) 12, 527, 671
BsmBI CGTCTC 1 cut(s) 671
BsmFI GGGAC 2 cut(s) 314, 412
BsoBI CYCGRG 2 cut(s) 15, 649
Bsp1286I GDGCHC 3 cut(s) 385, 889, 897
Bsp143I GATC 5 cut(s) 229, 655, 703, 827, 864
BspACI CCGC 4 cut(s) 359, 586, 632, 872
BspCNI CTCAG 3 cut(s) 205, 221, 803
BspDI ATCGAT 1 cut(s) 252
BspLI GGNNCC 3 cut(s) 270, 736, 849
BspPI GGATC 2 cut(s) 237, 835
BsrFI RCCGGY 2 cut(s) 779, 795
BsrI ACTGG 2 cut(s) 400, 424
BssAI RCCGGY 2 cut(s) 779, 795
BssECI CCNNGG 1 cut(s) 505
BssMI GATC 5 cut(s) 229, 655, 703, 827, 864
Bst2UI CCWGG 3 cut(s) 156, 290, 365
Bst4CI ACNGT 2 cut(s) 533, 559
Bst6I CTCTTC 1 cut(s) 122
BstAPI GCANNNNNTGC 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 3 cut(s) 208, 213, 790
BstDSI CCRYGG 1 cut(s) 505
BstF5I GGATG 3 cut(s) 65, 285, 418
BstKTI GATC 5 cut(s) 232, 658, 706, 830, 867
BstMAI GTCTC 3 cut(s) 12, 527, 671
BstMBI GATC 5 cut(s) 229, 655, 703, 827, 864
BstMCI CGRYCG 1 cut(s) 867
BstMWI GCNNNNNNNGC 2 cut(s) 74, 320
BstNI CCWGG 3 cut(s) 156, 290, 365
BstNSI RCATGY 1 cut(s) 768
BstSCI CCNGG 3 cut(s) 154, 288, 363
BstV1I GCAGC 4 cut(s) 136, 271, 338, 594
BstX2I RGATCY 2 cut(s) 229, 655
BstYI RGATCY 2 cut(s) 229, 655
Bsu15I ATCGAT 1 cut(s) 252
BsuTUI ATCGAT 1 cut(s) 252
BtgI CCRYGG 1 cut(s) 505
BtsCI GGATG 3 cut(s) 65, 285, 418
Cac8I GCNNGC 1 cut(s) 66
Cfr10I RCCGGY 2 cut(s) 779, 795
Cfr13I GGNCC 3 cut(s) 361, 734, 847
ClaI ATCGAT 1 cut(s) 252
CsiI ACCWGGT 2 cut(s) 154, 363
Csp6I GTAC 1 cut(s) 529
CspAI ACCGGT 2 cut(s) 779, 795
CviAII CATG 3 cut(s) 404, 409, 765
CviQI GTAC 1 cut(s) 529
DdeI CTNAG 3 cut(s) 208, 213, 790
DpnI GATC 5 cut(s) 231, 657, 705, 829, 866
DpnII GATC 5 cut(s) 229, 655, 703, 827, 864
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
EciI GGCGGA 1 cut(s) 621
Ecl136II GAGCTC 2 cut(s) 887, 895
Eco24I GRGCYC 2 cut(s) 889, 897
Eco32I GATATC 1 cut(s) 31
Eco47I GGWCC 3 cut(s) 361, 734, 847
Eco53kI GAGCTC 2 cut(s) 887, 895
Eco57I CTGAAG 4 cut(s) 74, 345, 675, 891
Eco88I CYCGRG 2 cut(s) 15, 649
EcoICRI GAGCTC 2 cut(s) 887, 895
EcoO109I RGGNCCY 1 cut(s) 734
EcoRII CCWGG 3 cut(s) 154, 288, 363
EcoRV GATATC 1 cut(s) 31
EcoT38I GRGCYC 2 cut(s) 889, 897
Esp3I CGTCTC 1 cut(s) 671
FaeI CATG 3 cut(s) 407, 412, 768
FalI AAGNNNNNCTT 2 cut(s) 61, 93
FaqI GGGAC 2 cut(s) 314, 412
FatI CATG 3 cut(s) 403, 408, 764
Fnu4HI GCNGC 5 cut(s) 150, 260, 352, 583, 586
FokI GGATG 3 cut(s) 72, 272, 425
FriOI GRGCYC 2 cut(s) 889, 897
Fsp4HI GCNGC 5 cut(s) 150, 260, 352, 583, 586
FspBI CTAG 2 cut(s) 440, 813
GluI GCNGC 5 cut(s) 150, 260, 352, 583, 586
GsuI CTGGAG 2 cut(s) 184, 369
HapII CCGG 2 cut(s) 780, 796
Hin1II CATG 3 cut(s) 407, 412, 768
HincII GTYRAC 1 cut(s) 536
HindII GTYRAC 1 cut(s) 536
HindIII AAGCTT 4 cut(s) 9, 83, 903, 916
HinfI GANTC 5 cut(s) 166, 178, 215, 788, 853
HpaII CCGG 2 cut(s) 780, 796
HphI GGTGA 1 cut(s) 782
Hpy166II GTNNAC 1 cut(s) 536
Hpy188I TCNGA 5 cut(s) 93, 318, 683, 793, 864
Hpy188III TCNNGA 8 cut(s) 15, 17, 175, 386, 707, 890, 898, 927
Hpy8I GTNNAC 1 cut(s) 536
HpyAV CCTTC 7 cut(s) 50, 113, 272, 470, 632, 699, 725
HpyCH4III ACNGT 2 cut(s) 533, 559
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 320
HpyF3I CTNAG 3 cut(s) 208, 213, 790
Hsp92II CATG 3 cut(s) 407, 412, 768
Kzo9I GATC 5 cut(s) 229, 655, 703, 827, 864
LmnI GCTCC 2 cut(s) 274, 388
Lsp1109I GCAGC 4 cut(s) 136, 271, 338, 594
LweI GCATC 1 cut(s) 670
MabI ACCWGGT 2 cut(s) 154, 363
MaeI CTAG 2 cut(s) 440, 813
MaeIII GTNAC 4 cut(s) 391, 457, 559, 770
MalI GATC 5 cut(s) 231, 657, 705, 829, 866
MboI GATC 5 cut(s) 229, 655, 703, 827, 864
MboII GAAGA 6 cut(s) 65, 80, 113, 139, 185, 224
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 2 cut(s) 229, 655
MhlI GDGCHC 3 cut(s) 385, 889, 897
MluCI AATT 8 cut(s) 104, 185, 273, 416, 485, 542, 589, 622
MlyI GAGTC 4 cut(s) 175, 187, 224, 782
MmeI TCCRAC 1 cut(s) 661
MnlI CCTC 5 cut(s) 123, 217, 246, 595, 870
MroXI GAANNNNTTC 2 cut(s) 10, 688
MseI TTAA 6 cut(s) 81, 108, 449, 471, 645, 776
MspA1I CMGCKG 1 cut(s) 212
MspI CCGG 2 cut(s) 780, 796
MspR9I CCNGG 3 cut(s) 156, 290, 365
MunI CAATTG 1 cut(s) 273
MvaI CCWGG 3 cut(s) 156, 290, 365
MwoI GCNNNNNNNGC 2 cut(s) 74, 320
NdeII GATC 5 cut(s) 229, 655, 703, 827, 864
NlaIII CATG 3 cut(s) 407, 412, 768
NlaIV GGNNCC 3 cut(s) 270, 736, 849
NmuCI GTSAC 1 cut(s) 770
NspI RCATGY 1 cut(s) 768
PaeR7I CTCGAG 1 cut(s) 15
PdmI GAANNNNTTC 2 cut(s) 10, 688
PfeI GAWTC 1 cut(s) 853
PflMI CCANNNNNTGG 1 cut(s) 844
PinAI ACCGGT 2 cut(s) 779, 795
PkrI GCNGC 5 cut(s) 151, 261, 353, 584, 587
Ple19I CGATCG 1 cut(s) 867
PleI GAGTC 4 cut(s) 174, 186, 223, 782
PpsI GAGTC 4 cut(s) 174, 186, 223, 782
PpuMI RGGWCCY 1 cut(s) 734
PsiI TTATAA 1 cut(s) 879
Psp124BI GAGCTC 2 cut(s) 889, 897
Psp5II RGGWCCY 1 cut(s) 734
Psp6I CCWGG 3 cut(s) 154, 288, 363
PspGI CCWGG 3 cut(s) 154, 288, 363
PspN4I GGNNCC 3 cut(s) 270, 736, 849
PspPI GGNCC 3 cut(s) 361, 734, 847
PspPPI RGGWCCY 1 cut(s) 734
PsuI RGATCY 2 cut(s) 229, 655
PvuI CGATCG 1 cut(s) 867
PvuII CAGCTG 1 cut(s) 212
RsaI GTAC 1 cut(s) 530
RsaNI GTAC 1 cut(s) 529
SacI GAGCTC 2 cut(s) 889, 897
SaqAI TTAA 6 cut(s) 81, 108, 449, 471, 645, 776
SatI GCNGC 5 cut(s) 150, 260, 352, 583, 586
Sau3AI GATC 5 cut(s) 229, 655, 703, 827, 864
Sau96I GGNCC 3 cut(s) 361, 734, 847
SchI GAGTC 4 cut(s) 175, 187, 224, 782
ScrFI CCNGG 3 cut(s) 156, 290, 365
SduI GDGCHC 3 cut(s) 385, 889, 897
SexAI ACCWGGT 2 cut(s) 154, 363
SfaNI GCATC 1 cut(s) 670
Sfr274I CTCGAG 1 cut(s) 15
SinI GGWCC 3 cut(s) 361, 734, 847
SlaI CTCGAG 1 cut(s) 15
SmlI CTYRAG 4 cut(s) 15, 175, 890, 896
SmoI CTYRAG 4 cut(s) 15, 175, 890, 896
Sse9I AATT 8 cut(s) 104, 185, 273, 416, 485, 542, 589, 622
SsiI CCGC 4 cut(s) 359, 586, 632, 872
SspI AATATT 1 cut(s) 112
SspMI CTAG 2 cut(s) 440, 813
SstI GAGCTC 2 cut(s) 889, 897
StyD4I CCNGG 3 cut(s) 154, 288, 363
TaaI ACNGT 2 cut(s) 533, 559
TaqI TCGA 3 cut(s) 16, 252, 708
TasI AATT 8 cut(s) 104, 185, 273, 416, 485, 542, 589, 622
TatI WGTACW 1 cut(s) 528
TauI GCSGC 1 cut(s) 588
TfiI GAWTC 1 cut(s) 853
Tru1I TTAA 6 cut(s) 81, 108, 449, 471, 645, 776
Tru9I TTAA 6 cut(s) 81, 108, 449, 471, 645, 776
TseFI GTSAC 1 cut(s) 770
TseI GCWGC 4 cut(s) 149, 259, 351, 582
Tsp45I GTSAC 1 cut(s) 770
TspDTI ATGAA 2 cut(s) 114, 429
TspGWI ACGGA 1 cut(s) 494
Van91I CCANNNNNTGG 1 cut(s) 844
VpaK11BI GGWCC 3 cut(s) 361, 734, 847
XapI RAATTY 3 cut(s) 104, 416, 485
XceI RCATGY 1 cut(s) 768
XhoI CTCGAG 1 cut(s) 15
XmnI GAANNNNTTC 2 cut(s) 10, 688
XspI CTAG 2 cut(s) 440, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.