Rroxscaffold_6G00416380

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0015906
Physical Location & Seq
Reverse (-)
38393883 .. 38396722
2840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00416380.1

Sequence Viewer

Length: 951 bp
ATGGGGAAAGCTTCAAGGGATAAGAGGGATATCTACTACCGAAAAGCCAAAGAAGAAGGCTGGCGAGCTCGGAGTGCCTTCAAGCTTCTTCAGATTGACGAGGAATTCAATATCTTCGATGGAGTGAAGCGCGTCGTCGACCTCTGTGCCGCCCCTGGTAGCTGGAGTCAGGTTTTGAGTAGGAAGTTGTATTTGCCGGCAAAGCTTTCGTCGGATTCTAAGGACGGGGATGTGCCCCTTATTGTAGCTATTGATTTGCAGCCCATGGCTCCAATTGAAGGTGTTCTTCAAGTGCAAGGTGATATAACGAATGCACGAACCGCCGAAGTAGTCATCAGACATTTTGATGGTTGCAAGGCTGATCTGGTTGTGTGTGACGGTGCTCCAGATGTTACTGGCCTTCATGACATGGATGAATTTGTTCAATCCCAGCTGATACTAGCAGGCTTAACTATTGTTACTCATGTACTTAAAGAAGGTGGTAAATTTATTGCAAAGATATTCCGTGGAAAAGATACTAGCCTTCTCTATTGTCAGCTGAAAGTATTTTTTCCGATTGTGACTTTTGCAAAACCAAAAAGCAGCCGCAATTCCAGCATAGAGGCATTTGCAGTTTGTGAGAATTATTCTCCTCCTGAGGGATTCAACCCTAAGGATCTGCATCGCCTCCTAGAAAAGGTAGGAAGTCCCTCAGGAGCAGATGATCTAGATTGCAGTAGTGGGTGGTTGGAAGGACCGAATAAGGTGTATATTCCATTTTTAGCTTGTGGTGACCTCAGTGGGTATGATTCTGACCGTTCATATCCACTGCCAAAAGATGCCAATGGAGCTTACCGAAGCTTGGATCCTGTACAGCCCCCAATTGCCCCTCCTTATAAGAGAGCTCTTGAACTGAAGAAAGCTTCCAGCCAGGGAATCACCGAGCTTGAGAACCACTCCCTAGAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

316

Amino Acids

34.74

Weight (kDa)

5.82

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 21 - 209 4.1e-49 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015906)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01230
fragaria_vesca FvH4_6g11910 FvH4_6g11910
rosa_chinensis RchiOBHm_Chr3g0463651 RchiOBHm_Chr5g0026681
rosa_laevigata RLG00000032946
rosa_multiflora Rmu_sc0003267.1_g000029
rosa_roxburghii Rroxscaffold_1G00052780 Rroxscaffold_6G00416380
rosa_rugosa Rorug03G0066800.1 Rorug05G0094700
rosa_samantha Rh3DG129100 Rh5BG184700 Rh5CG204600
rosa_wichuraiana Rw3G010470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 876
AccI GTMKAC 1 cut(s) 138
AccII CGCG 1 cut(s) 132
AciI CCGC 3 cut(s) 150, 321, 586
AclWI GGATC 3 cut(s) 663, 839, 852
AcsI RAATTY 3 cut(s) 104, 416, 485
AcuI CTGAAG 2 cut(s) 74, 914
AfaI GTAC 2 cut(s) 468, 852
AfiI CCNNNNNNNGG 4 cut(s) 278, 638, 676, 841
AgsI TTSAA 8 cut(s) 15, 82, 109, 278, 290, 425, 646, 890
AjnI CCWGG 2 cut(s) 154, 909
Alw21I GWGCWC 3 cut(s) 70, 385, 886
AlwI GGATC 3 cut(s) 663, 839, 852
AoxI GGCC 1 cut(s) 397
ApeKI GCWGC 2 cut(s) 259, 582
ApoI RAATTY 3 cut(s) 104, 416, 485
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
Asp700I GAANNNNTTC 2 cut(s) 282, 420
AspLEI GCGC 1 cut(s) 132
AspS9I GGNCC 1 cut(s) 734
AsuHPI GGTGA 3 cut(s) 311, 782, 910
AvaII GGWCC 1 cut(s) 734
AxyI CCTNAGG 3 cut(s) 636, 651, 691
BaeGI GKGCMC 1 cut(s) 237
BamHI GGATCC 1 cut(s) 844
BanII GRGCYC 2 cut(s) 70, 886
Bbv12I GWGCWC 3 cut(s) 70, 385, 886
BbvI GCAGC 2 cut(s) 271, 594
BccI CCATC 2 cut(s) 113, 341
BcgI CGANNNNNNTGC 4 cut(s) 128, 162, 189, 223
BciT130I CCWGG 2 cut(s) 156, 911
BfaI CTAG 5 cut(s) 440, 519, 671, 707, 941
BisI GCNGC 4 cut(s) 150, 260, 583, 586
BlsI GCNGC 4 cut(s) 151, 261, 584, 587
Bme1390I CCNGG 2 cut(s) 156, 911
Bme18I GGWCC 1 cut(s) 734
BmgT120I GGNCC 1 cut(s) 734
BmiI GGNNCC 2 cut(s) 270, 846
BmrFI CCNGG 2 cut(s) 156, 911
BmsI GCATC 2 cut(s) 670, 808
BplI GAGNNNNNCTC 1 cut(s) 920
BpmI CTGGAG 2 cut(s) 184, 369
BpuEI CTTGAG 1 cut(s) 947
BsaBI GATNNNNATC 1 cut(s) 660
BsaJI CCNNGG 4 cut(s) 154, 264, 505, 910
Bsc4I CCNNNNNNNGG 4 cut(s) 278, 638, 676, 841
Bse118I RCCGGY 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 400
Bse21I CCTNAGG 3 cut(s) 636, 651, 691
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 2 cut(s) 156, 911
BseDI CCNNGG 4 cut(s) 154, 264, 505, 910
BseGI GGATG 2 cut(s) 235, 418
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 4 cut(s) 278, 638, 676, 841
BseMII CTCAG 3 cut(s) 627, 705, 790
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 1 cut(s) 621
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 2 cut(s) 271, 594
BseYI CCCAGC 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 132
BshFI GGCC 1 cut(s) 399
BsiHKAI GWGCWC 3 cut(s) 70, 385, 886
BsiSI CCGG 1 cut(s) 197
BslFI GGGAC 1 cut(s) 672
BslI CCNNNNNNNGG 4 cut(s) 278, 638, 676, 841
BsmFI GGGAC 1 cut(s) 672
BsmI GAATGC 1 cut(s) 316
BsnI GGCC 1 cut(s) 399
Bsp1286I GDGCHC 4 cut(s) 70, 237, 385, 886
Bsp1407I TGTACA 1 cut(s) 850
Bsp143I GATC 4 cut(s) 361, 655, 703, 844
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 3 cut(s) 150, 321, 586
BspANI GGCC 1 cut(s) 399
BspCNI CTCAG 3 cut(s) 628, 704, 789
BspFNI CGCG 1 cut(s) 132
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 270, 846
BspPI GGATC 3 cut(s) 663, 839, 852
BsrFI RCCGGY 1 cut(s) 196
BsrGI TGTACA 1 cut(s) 850
BsrI ACTGG 1 cut(s) 400
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 4 cut(s) 154, 264, 505, 910
BssMI GATC 4 cut(s) 361, 655, 703, 844
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 2 cut(s) 156, 911
Bst4CI ACNGT 2 cut(s) 380, 797
BstAUI TGTACA 1 cut(s) 850
BstC8I GCNNGC 4 cut(s) 62, 66, 198, 445
BstDEI CTNAG 5 cut(s) 219, 636, 651, 691, 776
BstDSI CCRYGG 2 cut(s) 264, 505
BstEII GGTNACC 1 cut(s) 770
BstENI CCTNNNNNAGG 1 cut(s) 674
BstF5I GGATG 2 cut(s) 235, 418
BstFNI CGCG 1 cut(s) 132
BstHHI GCGC 1 cut(s) 132
BstKTI GATC 4 cut(s) 364, 658, 706, 847
BstMBI GATC 4 cut(s) 361, 655, 703, 844
BstMWI GCNNNNNNNGC 5 cut(s) 74, 202, 320, 594, 827
BstNI CCWGG 2 cut(s) 156, 911
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 2 cut(s) 154, 909
BstSLI GKGCMC 1 cut(s) 237
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 2 cut(s) 271, 594
BstX2I RGATCY 2 cut(s) 655, 844
BstYI RGATCY 2 cut(s) 655, 844
Bsu36I CCTNAGG 3 cut(s) 636, 651, 691
BsuRI GGCC 1 cut(s) 399
BtgI CCRYGG 2 cut(s) 264, 505
BtgZI GCGATG 1 cut(s) 647
BtsCI GGATG 2 cut(s) 235, 418
BtsI GCAGTG 1 cut(s) 806
BtsIMutI CAGTG 2 cut(s) 784, 806
Cac8I GCNNGC 4 cut(s) 62, 66, 198, 445
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 132
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 734
CseI GACGC 1 cut(s) 121
Csp6I GTAC 2 cut(s) 467, 851
CviAII CATG 4 cut(s) 265, 404, 409, 464
CviQI GTAC 2 cut(s) 467, 851
DdeI CTNAG 5 cut(s) 219, 636, 651, 691, 776
DpnI GATC 4 cut(s) 363, 657, 705, 846
DpnII GATC 4 cut(s) 361, 655, 703, 844
Ecl136II GAGCTC 2 cut(s) 68, 884
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 2 cut(s) 70, 886
Eco32I GATATC 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 734
Eco53kI GAGCTC 2 cut(s) 68, 884
Eco57I CTGAAG 2 cut(s) 74, 914
Eco81I CCTNAGG 3 cut(s) 636, 651, 691
Eco91I GGTNACC 1 cut(s) 770
EcoICRI GAGCTC 2 cut(s) 68, 884
EcoNI CCTNNNNNAGG 1 cut(s) 674
EcoO65I GGTNACC 1 cut(s) 770
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 2 cut(s) 154, 909
EcoRV GATATC 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 2 cut(s) 70, 886
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 4 cut(s) 268, 407, 412, 467
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FaqI GGGAC 1 cut(s) 672
FatI CATG 4 cut(s) 264, 403, 408, 463
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 4 cut(s) 150, 260, 583, 586
FokI GGATG 2 cut(s) 242, 425
FriOI GRGCYC 2 cut(s) 70, 886
Fsp4HI GCNGC 4 cut(s) 150, 260, 583, 586
FspBI CTAG 5 cut(s) 440, 519, 671, 707, 941
GlaI GCGC 1 cut(s) 131
GluI GCNGC 4 cut(s) 150, 260, 583, 586
GsaI CCCAGC 1 cut(s) 433
GsuI CTGGAG 2 cut(s) 184, 369
HaeIII GGCC 1 cut(s) 399
HapII CCGG 1 cut(s) 197
HgaI GACGC 1 cut(s) 121
HhaI GCGC 1 cut(s) 132
Hin1II CATG 4 cut(s) 268, 407, 412, 467
Hin6I GCGC 1 cut(s) 130
HinP1I GCGC 1 cut(s) 130
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HindIII AAGCTT 5 cut(s) 9, 83, 203, 838, 900
HinfI GANTC 6 cut(s) 166, 215, 642, 788, 915, 944
HpaII CCGG 1 cut(s) 197
HphI GGTGA 3 cut(s) 311, 782, 910
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 6 cut(s) 72, 93, 214, 338, 555, 793
Hpy188III TCNNGA 7 cut(s) 386, 404, 635, 693, 707, 887, 948
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 3 cut(s) 137, 140, 214
HpyAV CCTTC 7 cut(s) 50, 88, 272, 410, 470, 533, 725
HpyCH4III ACNGT 2 cut(s) 380, 797
HpyCH4V TGCA 9 cut(s) 259, 295, 314, 354, 494, 569, 611, 661, 714
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 202, 320, 594, 827
HpyF3I CTNAG 5 cut(s) 219, 636, 651, 691, 776
Hsp92II CATG 4 cut(s) 268, 407, 412, 467
HspAI GCGC 1 cut(s) 130
KroI GCCGGC 1 cut(s) 196
KroNI GCCGGC 1 cut(s) 198
Kzo9I GATC 4 cut(s) 361, 655, 703, 844
LmnI GCTCC 4 cut(s) 274, 388, 695, 827
Lsp1109I GCAGC 2 cut(s) 271, 594
LweI GCATC 2 cut(s) 670, 808
MaeI CTAG 5 cut(s) 440, 519, 671, 707, 941
MaeIII GTNAC 5 cut(s) 374, 391, 457, 559, 770
MalI GATC 4 cut(s) 363, 657, 705, 846
MboI GATC 4 cut(s) 361, 655, 703, 844
MboII GAAGA 5 cut(s) 65, 80, 106, 278, 907
MfeI CAATTG 2 cut(s) 273, 861
MflI RGATCY 2 cut(s) 655, 844
MhlI GDGCHC 4 cut(s) 70, 237, 385, 886
MluCI AATT 7 cut(s) 104, 273, 416, 485, 589, 622, 861
MlyI GAGTC 1 cut(s) 175
MmeI TCCRAC 2 cut(s) 192, 708
MroNI GCCGGC 1 cut(s) 196
MroXI GAANNNNTTC 2 cut(s) 282, 420
MseI TTAA 2 cut(s) 449, 471
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 2 cut(s) 433, 538
MspI CCGG 1 cut(s) 197
MspR9I CCNGG 2 cut(s) 156, 911
MunI CAATTG 2 cut(s) 273, 861
Mva1269I GAATGC 1 cut(s) 316
MvaI CCWGG 2 cut(s) 156, 911
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 5 cut(s) 74, 202, 320, 594, 827
NaeI GCCGGC 1 cut(s) 198
NcoI CCATGG 1 cut(s) 264
NdeII GATC 4 cut(s) 361, 655, 703, 844
NgoMIV GCCGGC 1 cut(s) 196
NlaIII CATG 4 cut(s) 268, 407, 412, 467
NlaIV GGNNCC 2 cut(s) 270, 846
NmuCI GTSAC 3 cut(s) 374, 559, 770
PagI TCATGA 1 cut(s) 403
PctI GAATGC 1 cut(s) 316
PdiI GCCGGC 1 cut(s) 198
PdmI GAANNNNTTC 2 cut(s) 282, 420
PfeI GAWTC 5 cut(s) 215, 642, 788, 915, 944
PkrI GCNGC 4 cut(s) 151, 261, 584, 587
PleI GAGTC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 174
PsiI TTATAA 1 cut(s) 876
Psp124BI GAGCTC 2 cut(s) 70, 886
Psp6I CCWGG 2 cut(s) 154, 909
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 1 cut(s) 429
PspGI CCWGG 2 cut(s) 154, 909
PspN4I GGNNCC 2 cut(s) 270, 846
PspPI GGNCC 1 cut(s) 734
PsuI RGATCY 2 cut(s) 655, 844
PvuII CAGCTG 2 cut(s) 433, 538
RsaI GTAC 2 cut(s) 468, 852
RsaNI GTAC 2 cut(s) 467, 851
RseI CAYNNNNRTG 1 cut(s) 345
SacI GAGCTC 2 cut(s) 70, 886
SalI GTCGAC 1 cut(s) 137
SaqAI TTAA 2 cut(s) 449, 471
SatI GCNGC 4 cut(s) 150, 260, 583, 586
Sau3AI GATC 4 cut(s) 361, 655, 703, 844
Sau96I GGNCC 1 cut(s) 734
SchI GAGTC 1 cut(s) 175
ScrFI CCNGG 2 cut(s) 156, 911
SduI GDGCHC 4 cut(s) 70, 237, 385, 886
SfaNI GCATC 2 cut(s) 670, 808
SinI GGWCC 1 cut(s) 734
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
Sse9I AATT 7 cut(s) 104, 273, 416, 485, 589, 622, 861
SsiI CCGC 3 cut(s) 150, 321, 586
SspMI CTAG 5 cut(s) 440, 519, 671, 707, 941
SstI GAGCTC 2 cut(s) 70, 886
StyD4I CCNGG 2 cut(s) 154, 909
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 2 cut(s) 380, 797
TaqI TCGA 2 cut(s) 117, 138
TaqII GACCGA 1 cut(s) 751
TasI AATT 7 cut(s) 104, 273, 416, 485, 589, 622, 861
TatI WGTACW 2 cut(s) 466, 850
TauI GCSGC 2 cut(s) 152, 588
TfiI GAWTC 5 cut(s) 215, 642, 788, 915, 944
Tru1I TTAA 2 cut(s) 449, 471
Tru9I TTAA 2 cut(s) 449, 471
TscAI CASTG 2 cut(s) 784, 813
TseFI GTSAC 3 cut(s) 374, 559, 770
TseI GCWGC 2 cut(s) 259, 582
Tsp45I GTSAC 3 cut(s) 374, 559, 770
TspDTI ATGAA 3 cut(s) 392, 429, 789
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 2 cut(s) 784, 813
VpaK11BI GGWCC 1 cut(s) 734
XagI CCTNNNNNAGG 1 cut(s) 674
XapI RAATTY 3 cut(s) 104, 416, 485
XbaI TCTAGA 1 cut(s) 706
XmiI GTMKAC 1 cut(s) 138
XmnI GAANNNNTTC 2 cut(s) 282, 420
XspI CTAG 5 cut(s) 440, 519, 671, 707, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.