Rw3G010470

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0015906
Physical Location & Seq
Forward (+)
10093985 .. 10096925
2941 bp
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UTR
Exon/CDS
Intron
Rw3G010470.1

Sequence Viewer

Length: 687 bp
ATGGCTCCAATTGAAGGTGTTATTCAAGTGCAAGGTGATATAACGAATGCACGAACCGCCGAAGTAGTCATCAGACATTTTGATGGTTGCAAGGCTGATCTGGTTGTGTGTGATGGTGCTCCAGATGTTACTGGCCTTCATGACATGGATGAATTTGTTCAATCCCAGCTGATACTAGCAGGCTTAACTATTGTTACTCATGTACTTAAAGAAGGTGGTAAATTTATTGCAAAGATATTCCGTGGAAAAGATACTAGTCTTCTCTATTGTCAGCTGAAAGTATTTTTTCCAATTGTGACTTTTGCAAAACCAAAAAGCAGCCGCAATTCCAGCATAGAGGCATTTGCAGTTTGTGAGAATTATTCTCCTCCTGAGGGATTCAACCCTAAGGATCTGCATCGCCTCCTAGAAAAGGTAGGAAGTCCCTCAGGAGCAGATGATCTAGATTGCAGTAGTGGGTGGTTGGAAGGACCGAATAAGGTGTATATTCCATTTTTAGCTTGTGGTGACCTCAGTGGGTATGATTCTGACCGTTCATATCCGCTGCCAAAAGATGCCAATGGAGCTTACCGAAGCTTGGATCCTGTACAGCCCCCAATTGCCCCTCCTTATAAGAGAGCTCTTGAACTGAAGAAAGCTTCCAGCCAGGGAATCACCGAGCTTGAGAACCACTCCCTGGAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

228

Amino Acids

24.78

Weight (kDa)

5.25

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 2 - 121 5e-26 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015906)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01230
fragaria_vesca FvH4_6g11910 FvH4_6g11910
rosa_chinensis RchiOBHm_Chr3g0463651 RchiOBHm_Chr5g0026681
rosa_laevigata RLG00000032946
rosa_multiflora Rmu_sc0003267.1_g000029
rosa_roxburghii Rroxscaffold_1G00052780 Rroxscaffold_6G00416380
rosa_rugosa Rorug03G0066800.1 Rorug05G0094700
rosa_samantha Rh3DG129100 Rh5BG184700 Rh5CG204600
rosa_wichuraiana Rw3G010470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
AccB7I CCANNNNNTGG 1 cut(s) 676
AciI CCGC 3 cut(s) 57, 322, 542
AclWI GGATC 3 cut(s) 399, 575, 588
AcsI RAATTY 2 cut(s) 152, 221
AcuI CTGAAG 1 cut(s) 650
AfaI GTAC 2 cut(s) 204, 588
AfiI CCNNNNNNNGG 5 cut(s) 14, 374, 412, 577, 676
AgsI TTSAA 5 cut(s) 14, 26, 161, 382, 626
AhlI ACTAGT 1 cut(s) 254
AjnI CCWGG 2 cut(s) 645, 675
AluBI AGCT 8 cut(s) 169, 274, 500, 566, 576, 620, 638, 661
AluI AGCT 8 cut(s) 169, 274, 500, 566, 576, 620, 638, 661
Alw21I GWGCWC 2 cut(s) 121, 622
AlwI GGATC 3 cut(s) 399, 575, 588
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 2 cut(s) 318, 544
ApoI RAATTY 2 cut(s) 152, 221
Asp700I GAANNNNTTC 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 3 cut(s) 47, 518, 646
AvaII GGWCC 1 cut(s) 470
AxyI CCTNAGG 3 cut(s) 372, 387, 427
BamHI GGATCC 1 cut(s) 580
BanII GRGCYC 1 cut(s) 622
BbsI GAAGAC 1 cut(s) 251
Bbv12I GWGCWC 2 cut(s) 121, 622
BbvI GCAGC 2 cut(s) 330, 531
BccI CCATC 2 cut(s) 77, 107
BciT130I CCWGG 2 cut(s) 647, 677
BcuI ACTAGT 1 cut(s) 254
BfaI CTAG 4 cut(s) 176, 255, 407, 443
BisI GCNGC 3 cut(s) 319, 322, 545
BlsI GCNGC 3 cut(s) 320, 323, 546
Bme1390I CCNGG 2 cut(s) 647, 677
Bme18I GGWCC 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 2 cut(s) 6, 582
BmrFI CCNGG 2 cut(s) 647, 677
BmsI GCATC 2 cut(s) 406, 544
BpiI GAAGAC 1 cut(s) 251
BplI GAGNNNNNCTC 1 cut(s) 656
BpmI CTGGAG 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 683
BsaBI GATNNNNATC 1 cut(s) 396
BsaJI CCNNGG 3 cut(s) 241, 646, 675
Bsc4I CCNNNNNNNGG 5 cut(s) 14, 374, 412, 577, 676
Bse1I ACTGG 1 cut(s) 136
Bse21I CCTNAGG 3 cut(s) 372, 387, 427
Bse8I GATNNNNATC 1 cut(s) 396
BseBI CCWGG 2 cut(s) 647, 677
BseDI CCNNGG 3 cut(s) 241, 646, 675
BseGI GGATG 1 cut(s) 154
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 5 cut(s) 14, 374, 412, 577, 676
BseMII CTCAG 3 cut(s) 363, 441, 526
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 1 cut(s) 357
BseXI GCAGC 2 cut(s) 330, 531
BseYI CCCAGC 1 cut(s) 165
BshFI GGCC 1 cut(s) 135
BsiHKAI GWGCWC 2 cut(s) 121, 622
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 5 cut(s) 14, 374, 412, 577, 676
BsmFI GGGAC 1 cut(s) 408
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 1 cut(s) 135
Bsp1286I GDGCHC 2 cut(s) 121, 622
Bsp1407I TGTACA 1 cut(s) 586
Bsp143I GATC 4 cut(s) 97, 391, 439, 580
BspACI CCGC 3 cut(s) 57, 322, 542
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 3 cut(s) 364, 440, 525
BspHI TCATGA 1 cut(s) 139
BspLI GGNNCC 2 cut(s) 6, 582
BspPI GGATC 3 cut(s) 399, 575, 588
BsrGI TGTACA 1 cut(s) 586
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 3 cut(s) 241, 646, 675
BssMI GATC 4 cut(s) 97, 391, 439, 580
Bst2UI CCWGG 2 cut(s) 647, 677
Bst4CI ACNGT 1 cut(s) 533
BstAUI TGTACA 1 cut(s) 586
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 4 cut(s) 372, 387, 427, 512
BstDSI CCRYGG 1 cut(s) 241
BstEII GGTNACC 1 cut(s) 506
BstENI CCTNNNNNAGG 1 cut(s) 410
BstF5I GGATG 1 cut(s) 154
BstKTI GATC 4 cut(s) 100, 394, 442, 583
BstMBI GATC 4 cut(s) 97, 391, 439, 580
BstMWI GCNNNNNNNGC 3 cut(s) 56, 330, 563
BstNI CCWGG 2 cut(s) 647, 677
BstPI GGTNACC 1 cut(s) 506
BstSCI CCNGG 2 cut(s) 645, 675
BstV1I GCAGC 2 cut(s) 330, 531
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 2 cut(s) 391, 580
BstYI RGATCY 2 cut(s) 391, 580
Bsu36I CCTNAGG 3 cut(s) 372, 387, 427
BsuRI GGCC 1 cut(s) 135
BtgI CCRYGG 1 cut(s) 241
BtgZI GCGATG 1 cut(s) 383
BtsCI GGATG 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 520
Cac8I GCNNGC 1 cut(s) 181
CciI TCATGA 1 cut(s) 139
Cfr13I GGNCC 1 cut(s) 470
Csp6I GTAC 2 cut(s) 203, 587
CviAII CATG 3 cut(s) 140, 145, 200
CviQI GTAC 2 cut(s) 203, 587
DdeI CTNAG 4 cut(s) 372, 387, 427, 512
DpnI GATC 4 cut(s) 99, 393, 441, 582
DpnII GATC 4 cut(s) 97, 391, 439, 580
Ecl136II GAGCTC 1 cut(s) 620
Eco24I GRGCYC 1 cut(s) 622
Eco47I GGWCC 1 cut(s) 470
Eco53kI GAGCTC 1 cut(s) 620
Eco57I CTGAAG 1 cut(s) 650
Eco81I CCTNAGG 3 cut(s) 372, 387, 427
Eco91I GGTNACC 1 cut(s) 506
EcoICRI GAGCTC 1 cut(s) 620
EcoNI CCTNNNNNAGG 1 cut(s) 410
EcoO65I GGTNACC 1 cut(s) 506
EcoRII CCWGG 2 cut(s) 645, 675
EcoT38I GRGCYC 1 cut(s) 622
FaeI CATG 3 cut(s) 143, 148, 203
FaiI YATR 9 cut(s) 41, 141, 146, 201, 335, 486, 522, 538, 612
FaqI GGGAC 1 cut(s) 408
FatI CATG 3 cut(s) 139, 144, 199
Fnu4HI GCNGC 3 cut(s) 319, 322, 545
FokI GGATG 1 cut(s) 161
FriOI GRGCYC 1 cut(s) 622
Fsp4HI GCNGC 3 cut(s) 319, 322, 545
FspBI CTAG 4 cut(s) 176, 255, 407, 443
GluI GCNGC 3 cut(s) 319, 322, 545
GsaI CCCAGC 1 cut(s) 169
GsuI CTGGAG 1 cut(s) 105
HaeIII GGCC 1 cut(s) 135
Hin1II CATG 3 cut(s) 143, 148, 203
HindIII AAGCTT 2 cut(s) 574, 636
HinfI GANTC 4 cut(s) 378, 524, 651, 680
HphI GGTGA 3 cut(s) 47, 518, 646
Hpy188I TCNGA 2 cut(s) 74, 529
Hpy188III TCNNGA 7 cut(s) 122, 140, 371, 429, 443, 623, 684
HpyAV CCTTC 4 cut(s) 8, 146, 206, 461
HpyCH4III ACNGT 1 cut(s) 533
HpyCH4V TGCA 8 cut(s) 31, 50, 90, 230, 305, 347, 397, 450
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 330, 563
HpyF3I CTNAG 4 cut(s) 372, 387, 427, 512
Hsp92II CATG 3 cut(s) 143, 148, 203
Kzo9I GATC 4 cut(s) 97, 391, 439, 580
LmnI GCTCC 4 cut(s) 10, 124, 431, 563
Lsp1109I GCAGC 2 cut(s) 330, 531
LweI GCATC 2 cut(s) 406, 544
MaeI CTAG 4 cut(s) 176, 255, 407, 443
MaeIII GTNAC 4 cut(s) 127, 193, 295, 506
MalI GATC 4 cut(s) 99, 393, 441, 582
MboI GATC 4 cut(s) 97, 391, 439, 580
MboII GAAGA 2 cut(s) 251, 643
MfeI CAATTG 3 cut(s) 9, 291, 597
MflI RGATCY 2 cut(s) 391, 580
MhlI GDGCHC 2 cut(s) 121, 622
MluCI AATT 7 cut(s) 9, 152, 221, 291, 325, 358, 597
MmeI TCCRAC 1 cut(s) 444
MnlI CCTC 7 cut(s) 331, 367, 378, 413, 436, 521, 615
MroXI GAANNNNTTC 1 cut(s) 156
MseI TTAA 2 cut(s) 185, 207
MslI CAYNNNNRTG 1 cut(s) 81
MspA1I CMGCKG 3 cut(s) 169, 274, 544
MspR9I CCNGG 2 cut(s) 647, 677
MunI CAATTG 3 cut(s) 9, 291, 597
Mva1269I GAATGC 1 cut(s) 52
MvaI CCWGG 2 cut(s) 647, 677
MwoI GCNNNNNNNGC 3 cut(s) 56, 330, 563
NdeII GATC 4 cut(s) 97, 391, 439, 580
NlaIII CATG 3 cut(s) 143, 148, 203
NlaIV GGNNCC 2 cut(s) 6, 582
NmuCI GTSAC 2 cut(s) 295, 506
PagI TCATGA 1 cut(s) 139
PctI GAATGC 1 cut(s) 52
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 4 cut(s) 378, 524, 651, 680
PflMI CCANNNNNTGG 1 cut(s) 676
PkrI GCNGC 3 cut(s) 320, 323, 546
PsiI TTATAA 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 622
Psp6I CCWGG 2 cut(s) 645, 675
PspEI GGTNACC 1 cut(s) 506
PspFI CCCAGC 1 cut(s) 165
PspGI CCWGG 2 cut(s) 645, 675
PspN4I GGNNCC 2 cut(s) 6, 582
PspPI GGNCC 1 cut(s) 470
PsuI RGATCY 2 cut(s) 391, 580
PvuII CAGCTG 2 cut(s) 169, 274
RsaI GTAC 2 cut(s) 204, 588
RsaNI GTAC 2 cut(s) 203, 587
RseI CAYNNNNRTG 1 cut(s) 81
SacI GAGCTC 1 cut(s) 622
SaqAI TTAA 2 cut(s) 185, 207
SatI GCNGC 3 cut(s) 319, 322, 545
Sau3AI GATC 4 cut(s) 97, 391, 439, 580
Sau96I GGNCC 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 647, 677
SduI GDGCHC 2 cut(s) 121, 622
SfaNI GCATC 2 cut(s) 406, 544
SinI GGWCC 1 cut(s) 470
SmiMI CAYNNNNRTG 1 cut(s) 81
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
SpeI ACTAGT 1 cut(s) 254
Sse9I AATT 7 cut(s) 9, 152, 221, 291, 325, 358, 597
SsiI CCGC 3 cut(s) 57, 322, 542
SspMI CTAG 4 cut(s) 176, 255, 407, 443
SstI GAGCTC 1 cut(s) 622
StyD4I CCNGG 2 cut(s) 645, 675
TaaI ACNGT 1 cut(s) 533
TaqII GACCGA 1 cut(s) 487
TasI AATT 7 cut(s) 9, 152, 221, 291, 325, 358, 597
TatI WGTACW 2 cut(s) 202, 586
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 4 cut(s) 378, 524, 651, 680
Tru1I TTAA 2 cut(s) 185, 207
Tru9I TTAA 2 cut(s) 185, 207
TscAI CASTG 1 cut(s) 520
TseFI GTSAC 2 cut(s) 295, 506
TseI GCWGC 2 cut(s) 318, 544
Tsp45I GTSAC 2 cut(s) 295, 506
TspDTI ATGAA 3 cut(s) 128, 165, 525
TspGWI ACGGA 1 cut(s) 230
TspRI CASTG 1 cut(s) 520
Van91I CCANNNNNTGG 1 cut(s) 676
VpaK11BI GGWCC 1 cut(s) 470
XagI CCTNNNNNAGG 1 cut(s) 410
XapI RAATTY 2 cut(s) 152, 221
XbaI TCTAGA 1 cut(s) 442
XmnI GAANNNNTTC 1 cut(s) 156
XspI CTAG 4 cut(s) 176, 255, 407, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.