RLG00000032946

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015906
Physical Location & Seq
Reverse (-)
21255629 .. 21258229
2601 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032946

Sequence Viewer

Length: 951 bp
ATGGGGAAAGCTTCAAGAGATAAGAGGGATATCTACTATCGAAAAGCCAAAGAAGAAGGCTGGCGAGCTCGCAGTGCCTTCAAGCTTCTTCAGATTGACGACGAATTCAACATCTTTGATGGAGTCAAGCGCGTCGTTGATCTCTGTGCCGCCCCTGGTAGCTGGAGTCAGGTTTTGAGTAGGAAGTTGTATCTCCCAGCAAAGCTTTCATCTGATTCTAAGGACGGGGATGTGCCCCTTATTGTAGCTATTGATTTGCAGCCAATGGCTCCAATTGAAGGTGTTATTCAAGTGCAGGGTGATATAACCAATGCACGAACCGCCGAAGTAGTCATCAGACATTTTGATGGTTGCAAGGCTGATCTGGTTGTGTGTGATGGTGCTCCAGATGTTACTGGCCTTCATGACATGGATGAATTTGTTCAGTCCCAGCTGATACTAGCAGGGTTAACAATTGTTACTCATGTACTTAAAGAAGGTGGTAAGTTTATTGCAAAGATATTCCGTGGAAAAGATACTAGTCTGCTCTATTGTCAGCTGAAAGTATTTTTCCCGATTGTGACTTTTGCAAAACCAAAAAGCAGTCGCAATTCGAGCATAGAGGCATTTGCAGTTTGTGAGAATTATTCTCCTCCTGAGGGATTCAACCCTAAGGATCTGCATCGCCTCCTAGAAAAGGTGGGAAGTCCCTCAGGAGCAGGTGATCTAGATTGCAGTAGTGGGTGGTTGGAAGGACCGAATAAGGTGTATATCCCATTTTTAGCTTGTGGTGACCTCAGTGGGTATGATTCTGACCGTTCATATCCGCTGCCAAAAGATGCCAATGGAGCTTACCGAAGCTTGGATCCTGTACAGCCCCCAATTGCCCCTCCTTATAAGAGAGCTCTTGAACTGAAGAAAGCTTCCAGCCAGGGACTCACCGAGCTTGAGAACCACTCCCTGGAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

317

Amino Acids

34.67

Weight (kDa)

6.02

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 21 - 209 1.1e-49 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015906)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01230
fragaria_vesca FvH4_6g11910 FvH4_6g11910
rosa_chinensis RchiOBHm_Chr3g0463651 RchiOBHm_Chr5g0026681
rosa_laevigata RLG00000032946
rosa_multiflora Rmu_sc0003267.1_g000029
rosa_roxburghii Rroxscaffold_1G00052780 Rroxscaffold_6G00416380
rosa_rugosa Rorug03G0066800.1 Rorug05G0094700
rosa_samantha Rh3DG129100 Rh5BG184700 Rh5CG204600
rosa_wichuraiana Rw3G010470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 876
AarI CACCTGC 1 cut(s) 689
Acc36I ACCTGC 1 cut(s) 689
AccB7I CCANNNNNTGG 1 cut(s) 940
AccII CGCG 1 cut(s) 132
AciI CCGC 3 cut(s) 150, 321, 806
AclWI GGATC 3 cut(s) 663, 839, 852
AcsI RAATTY 2 cut(s) 104, 416
AcuI CTGAAG 2 cut(s) 74, 914
AfaI GTAC 2 cut(s) 468, 852
AfiI CCNNNNNNNGG 5 cut(s) 278, 638, 676, 841, 940
AgsI TTSAA 7 cut(s) 15, 82, 109, 278, 290, 646, 890
AhlI ACTAGT 1 cut(s) 518
AjnI CCWGG 3 cut(s) 154, 909, 939
Alw21I GWGCWC 3 cut(s) 70, 385, 886
AlwI GGATC 3 cut(s) 663, 839, 852
AoxI GGCC 1 cut(s) 397
ApeKI GCWGC 2 cut(s) 259, 808
ApoI RAATTY 2 cut(s) 104, 416
Asp700I GAANNNNTTC 1 cut(s) 420
AspLEI GCGC 1 cut(s) 132
AspS9I GGNCC 1 cut(s) 734
AsuHPI GGTGA 4 cut(s) 311, 713, 782, 910
AvaII GGWCC 1 cut(s) 734
AxyI CCTNAGG 3 cut(s) 636, 651, 691
BaeGI GKGCMC 1 cut(s) 237
BamHI GGATCC 1 cut(s) 844
BanII GRGCYC 2 cut(s) 70, 886
Bbv12I GWGCWC 3 cut(s) 70, 385, 886
BbvI GCAGC 2 cut(s) 271, 795
BccI CCATC 3 cut(s) 113, 341, 371
BciT130I CCWGG 3 cut(s) 156, 911, 941
BcuI ACTAGT 1 cut(s) 518
BfaI CTAG 4 cut(s) 440, 519, 671, 707
BfuAI ACCTGC 1 cut(s) 689
BisI GCNGC 3 cut(s) 150, 260, 809
BlsI GCNGC 3 cut(s) 151, 261, 810
Bme1390I CCNGG 3 cut(s) 156, 911, 941
Bme18I GGWCC 1 cut(s) 734
BmgT120I GGNCC 1 cut(s) 734
BmiI GGNNCC 2 cut(s) 270, 846
BmrFI CCNGG 3 cut(s) 156, 911, 941
BmsI GCATC 2 cut(s) 670, 808
BplI GAGNNNNNCTC 1 cut(s) 920
BpmI CTGGAG 2 cut(s) 184, 369
BpuEI CTTGAG 1 cut(s) 947
BsaBI GATNNNNATC 1 cut(s) 660
BsaJI CCNNGG 4 cut(s) 154, 505, 910, 939
Bsc4I CCNNNNNNNGG 5 cut(s) 278, 638, 676, 841, 940
Bse1I ACTGG 1 cut(s) 400
Bse21I CCTNAGG 3 cut(s) 636, 651, 691
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 3 cut(s) 156, 911, 941
BseDI CCNNGG 4 cut(s) 154, 505, 910, 939
BseGI GGATG 2 cut(s) 235, 418
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 5 cut(s) 278, 638, 676, 841, 940
BseMII CTCAG 3 cut(s) 627, 705, 790
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 1 cut(s) 621
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 2 cut(s) 271, 795
BseYI CCCAGC 2 cut(s) 196, 429
BsgI GTGCAG 1 cut(s) 314
Bsh1236I CGCG 1 cut(s) 132
BshFI GGCC 1 cut(s) 399
BsiHKAI GWGCWC 3 cut(s) 70, 385, 886
BslFI GGGAC 3 cut(s) 412, 672, 927
BslI CCNNNNNNNGG 5 cut(s) 278, 638, 676, 841, 940
BsmFI GGGAC 3 cut(s) 412, 672, 927
BsnI GGCC 1 cut(s) 399
Bsp1286I GDGCHC 4 cut(s) 70, 237, 385, 886
Bsp1407I TGTACA 1 cut(s) 850
Bsp143I GATC 5 cut(s) 139, 361, 655, 703, 844
BspACI CCGC 3 cut(s) 150, 321, 806
BspANI GGCC 1 cut(s) 399
BspCNI CTCAG 3 cut(s) 628, 704, 789
BspFNI CGCG 1 cut(s) 132
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 270, 846
BspMI ACCTGC 1 cut(s) 689
BspPI GGATC 3 cut(s) 663, 839, 852
BsrGI TGTACA 1 cut(s) 850
BsrI ACTGG 1 cut(s) 400
BssECI CCNNGG 4 cut(s) 154, 505, 910, 939
BssMI GATC 5 cut(s) 139, 361, 655, 703, 844
Bst2UI CCWGG 3 cut(s) 156, 911, 941
Bst4CI ACNGT 1 cut(s) 797
BstAUI TGTACA 1 cut(s) 850
BstC8I GCNNGC 3 cut(s) 62, 66, 70
BstDEI CTNAG 5 cut(s) 219, 636, 651, 691, 776
BstDSI CCRYGG 1 cut(s) 505
BstEII GGTNACC 1 cut(s) 770
BstENI CCTNNNNNAGG 1 cut(s) 674
BstF5I GGATG 2 cut(s) 235, 418
BstFNI CGCG 1 cut(s) 132
BstHHI GCGC 1 cut(s) 132
BstKTI GATC 5 cut(s) 142, 364, 658, 706, 847
BstMBI GATC 5 cut(s) 139, 361, 655, 703, 844
BstMWI GCNNNNNNNGC 4 cut(s) 74, 320, 594, 827
BstNI CCWGG 3 cut(s) 156, 911, 941
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 3 cut(s) 154, 909, 939
BstSLI GKGCMC 1 cut(s) 237
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 2 cut(s) 271, 795
BstX2I RGATCY 2 cut(s) 655, 844
BstYI RGATCY 2 cut(s) 655, 844
Bsu36I CCTNAGG 3 cut(s) 636, 651, 691
BsuRI GGCC 1 cut(s) 399
BtgI CCRYGG 1 cut(s) 505
BtgZI GCGATG 1 cut(s) 647
BtsCI GGATG 2 cut(s) 235, 418
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 2 cut(s) 79, 784
BveI ACCTGC 1 cut(s) 689
Cac8I GCNNGC 3 cut(s) 62, 66, 70
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 132
Cfr13I GGNCC 1 cut(s) 734
CseI GACGC 1 cut(s) 121
Csp6I GTAC 2 cut(s) 467, 851
CviAII CATG 3 cut(s) 404, 409, 464
CviQI GTAC 2 cut(s) 467, 851
DdeI CTNAG 5 cut(s) 219, 636, 651, 691, 776
DpnI GATC 5 cut(s) 141, 363, 657, 705, 846
DpnII GATC 5 cut(s) 139, 361, 655, 703, 844
Ecl136II GAGCTC 2 cut(s) 68, 884
Eco24I GRGCYC 2 cut(s) 70, 886
Eco32I GATATC 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 734
Eco53kI GAGCTC 2 cut(s) 68, 884
Eco57I CTGAAG 2 cut(s) 74, 914
Eco81I CCTNAGG 3 cut(s) 636, 651, 691
Eco91I GGTNACC 1 cut(s) 770
EcoICRI GAGCTC 2 cut(s) 68, 884
EcoNI CCTNNNNNAGG 1 cut(s) 674
EcoO65I GGTNACC 1 cut(s) 770
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 3 cut(s) 154, 909, 939
EcoRV GATATC 1 cut(s) 31
EcoT38I GRGCYC 2 cut(s) 70, 886
FaeI CATG 3 cut(s) 407, 412, 467
FaiI YATR 9 cut(s) 305, 405, 410, 465, 599, 750, 786, 802, 876
FaqI GGGAC 3 cut(s) 412, 672, 927
FatI CATG 3 cut(s) 403, 408, 463
Fnu4HI GCNGC 3 cut(s) 150, 260, 809
FokI GGATG 2 cut(s) 242, 425
FriOI GRGCYC 2 cut(s) 70, 886
Fsp4HI GCNGC 3 cut(s) 150, 260, 809
FspBI CTAG 4 cut(s) 440, 519, 671, 707
GlaI GCGC 1 cut(s) 131
GluI GCNGC 3 cut(s) 150, 260, 809
GsaI CCCAGC 2 cut(s) 200, 433
GsuI CTGGAG 2 cut(s) 184, 369
HaeIII GGCC 1 cut(s) 399
HgaI GACGC 1 cut(s) 121
HhaI GCGC 1 cut(s) 132
Hin1II CATG 3 cut(s) 407, 412, 467
Hin6I GCGC 1 cut(s) 130
HinP1I GCGC 1 cut(s) 130
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HindIII AAGCTT 5 cut(s) 9, 83, 203, 838, 900
HinfI GANTC 7 cut(s) 123, 166, 215, 642, 788, 915, 944
HpaI GTTAAC 1 cut(s) 450
HphI GGTGA 4 cut(s) 311, 713, 782, 910
Hpy166II GTNNAC 1 cut(s) 450
Hpy188I TCNGA 4 cut(s) 93, 214, 338, 793
Hpy188III TCNNGA 9 cut(s) 15, 386, 404, 553, 635, 693, 707, 887, 948
Hpy8I GTNNAC 1 cut(s) 450
Hpy99I CGWCG 2 cut(s) 104, 137
HpyAV CCTTC 6 cut(s) 50, 88, 272, 410, 470, 725
HpyCH4III ACNGT 1 cut(s) 797
HpyCH4V TGCA 9 cut(s) 259, 295, 314, 354, 494, 569, 611, 661, 714
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 320, 594, 827
HpyF3I CTNAG 5 cut(s) 219, 636, 651, 691, 776
Hsp92II CATG 3 cut(s) 407, 412, 467
HspAI GCGC 1 cut(s) 130
KspAI GTTAAC 1 cut(s) 450
Kzo9I GATC 5 cut(s) 139, 361, 655, 703, 844
LmnI GCTCC 4 cut(s) 274, 388, 695, 827
Lsp1109I GCAGC 2 cut(s) 271, 795
LweI GCATC 2 cut(s) 670, 808
MaeI CTAG 4 cut(s) 440, 519, 671, 707
MaeIII GTNAC 4 cut(s) 391, 457, 559, 770
MalI GATC 5 cut(s) 141, 363, 657, 705, 846
MboI GATC 5 cut(s) 139, 361, 655, 703, 844
MboII GAAGA 3 cut(s) 65, 80, 907
MfeI CAATTG 3 cut(s) 273, 453, 861
MflI RGATCY 2 cut(s) 655, 844
MhlI GDGCHC 4 cut(s) 70, 237, 385, 886
MluCI AATT 7 cut(s) 104, 273, 416, 453, 589, 622, 861
MlyI GAGTC 3 cut(s) 132, 175, 909
MmeI TCCRAC 1 cut(s) 708
MnlI CCTC 8 cut(s) 18, 595, 631, 642, 677, 700, 785, 879
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 2 cut(s) 449, 471
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 3 cut(s) 433, 538, 808
MspR9I CCNGG 3 cut(s) 156, 911, 941
MunI CAATTG 3 cut(s) 273, 453, 861
MvaI CCWGG 3 cut(s) 156, 911, 941
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 4 cut(s) 74, 320, 594, 827
NdeII GATC 5 cut(s) 139, 361, 655, 703, 844
NlaIII CATG 3 cut(s) 407, 412, 467
NlaIV GGNNCC 2 cut(s) 270, 846
NmuCI GTSAC 2 cut(s) 559, 770
PagI TCATGA 1 cut(s) 403
PaqCI CACCTGC 1 cut(s) 689
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 4 cut(s) 215, 642, 788, 944
PflMI CCANNNNNTGG 1 cut(s) 940
PkrI GCNGC 3 cut(s) 151, 261, 810
PleI GAGTC 3 cut(s) 131, 174, 909
PpsI GAGTC 3 cut(s) 131, 174, 909
PsiI TTATAA 1 cut(s) 876
Psp124BI GAGCTC 2 cut(s) 70, 886
Psp6I CCWGG 3 cut(s) 154, 909, 939
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 2 cut(s) 196, 429
PspGI CCWGG 3 cut(s) 154, 909, 939
PspN4I GGNNCC 2 cut(s) 270, 846
PspPI GGNCC 1 cut(s) 734
PsuI RGATCY 2 cut(s) 655, 844
PvuII CAGCTG 2 cut(s) 433, 538
RsaI GTAC 2 cut(s) 468, 852
RsaNI GTAC 2 cut(s) 467, 851
RseI CAYNNNNRTG 1 cut(s) 345
SacI GAGCTC 2 cut(s) 70, 886
SaqAI TTAA 2 cut(s) 449, 471
SatI GCNGC 3 cut(s) 150, 260, 809
Sau3AI GATC 5 cut(s) 139, 361, 655, 703, 844
Sau96I GGNCC 1 cut(s) 734
SchI GAGTC 3 cut(s) 132, 175, 909
ScrFI CCNGG 3 cut(s) 156, 911, 941
SduI GDGCHC 4 cut(s) 70, 237, 385, 886
SfaNI GCATC 2 cut(s) 670, 808
SinI GGWCC 1 cut(s) 734
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
SpeI ACTAGT 1 cut(s) 518
Sse9I AATT 7 cut(s) 104, 273, 416, 453, 589, 622, 861
SsiI CCGC 3 cut(s) 150, 321, 806
SspMI CTAG 4 cut(s) 440, 519, 671, 707
SstI GAGCTC 2 cut(s) 70, 886
StyD4I CCNGG 3 cut(s) 154, 909, 939
TaaI ACNGT 1 cut(s) 797
TaqI TCGA 2 cut(s) 40, 593
TaqII GACCGA 1 cut(s) 751
TasI AATT 7 cut(s) 104, 273, 416, 453, 589, 622, 861
TatI WGTACW 2 cut(s) 466, 850
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 4 cut(s) 215, 642, 788, 944
Tru1I TTAA 2 cut(s) 449, 471
Tru9I TTAA 2 cut(s) 449, 471
TscAI CASTG 2 cut(s) 79, 784
TseFI GTSAC 2 cut(s) 559, 770
TseI GCWGC 2 cut(s) 259, 808
Tsp45I GTSAC 2 cut(s) 559, 770
TspDTI ATGAA 4 cut(s) 198, 392, 429, 789
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 2 cut(s) 79, 784
Van91I CCANNNNNTGG 1 cut(s) 940
VpaK11BI GGWCC 1 cut(s) 734
XagI CCTNNNNNAGG 1 cut(s) 674
XapI RAATTY 2 cut(s) 104, 416
XbaI TCTAGA 1 cut(s) 706
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 4 cut(s) 440, 519, 671, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.