FvH4_6g11910

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015906
Physical Location & Seq
Forward (+)
7134731 .. 7137789
3059 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g11910.t1

Sequence Viewer

Length: 951 bp
ATGGGGAAAGCTTCAAGGGATAAGAGGGATATCTACTACCGAAAAGCCAAAGAAGAAGGGTGGCGTGCTCGGAGTGCCTTCAAGCTTCTTCAGATTGATGAGGAGTTCAACATCTTCGACGGAGTCAAGCGCGTCGTCGATCTCTGTGCTGCTCCTGGTAGCTGGAGTCAGGTTTTGAGTAGGAAATTGTATCTCCCGGCAAAGCTTTCGTCTGATTCTAAGAACGGCGATGTGCCTCTTATTGTAGCTATTGATTTACAGCCAATGGCTCCGATTGAAGGTGTTATTCAAGTGCAGGGTGATATAACTAATGCACGAACCGCCGAAGTAGTCATCAGACATTTTGATGGTTGCAAGGCGGATCTGGTTGTGTGTGATGGTGCTCCAGACGTTACTGGCCTTCATGACATGGATGAATTTGTGCAGTCCCAGCTGATACTAGCAGGTTTAACAATTGTTACTCATGTACTCAAAGAAGGTGGTAAATTTATTGCAAAGATATTCCGTGGAAAAGATACAAGTCTTCTCTATTGTCAGCTGAAGGTATTTTTCCCGATTGTGACTTTTGCAAAACCAAAAAGCAGCCGCAATTCCAGCATAGAGGCATTTGCAGTTTGTGAGAATTATTCTCCTCCGGAGGGATTCGACCCTAAGGATCTGCATCGCCTCCTAGAAAAGGTGGGAACTCCCTCAGGAGCACATGATCTAGATTGCAGTAGTGGGTGGTTGGAAGGACCAAATAAGGTGTATATCCCATTTTTAGCTTGTGGTGACCTCAGTGGGTATGATTCTGACCGTTCATATCCGCTGCCAAAAGATGCCAATGGAGCTTACCGGAGCTTGGATCCTGTACAGCCCCCAATTGCTCCTCCTTATAAGAGAGCTCTTGAACTGAAGAAAGCTTCCAGCCAGGGAATCACCGAGCTTGAGAACCACTCCCTGGAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

317

Amino Acids

34.77

Weight (kDa)

6.11

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 21 - 209 8.4e-50 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015906)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01230
fragaria_vesca FvH4_6g11910 FvH4_6g11910
rosa_chinensis RchiOBHm_Chr3g0463651 RchiOBHm_Chr5g0026681
rosa_laevigata RLG00000032946
rosa_multiflora Rmu_sc0003267.1_g000029
rosa_roxburghii Rroxscaffold_1G00052780 Rroxscaffold_6G00416380
rosa_rugosa Rorug03G0066800.1 Rorug05G0094700
rosa_samantha Rh3DG129100 Rh5BG184700 Rh5CG204600
rosa_wichuraiana Rw3G010470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 876
Acc36I ACCTGC 1 cut(s) 434
AccB7I CCANNNNNTGG 1 cut(s) 940
AccII CGCG 1 cut(s) 132
AccIII TCCGGA 1 cut(s) 634
AciI CCGC 4 cut(s) 321, 359, 586, 806
AclWI GGATC 4 cut(s) 369, 663, 839, 852
AcsI RAATTY 2 cut(s) 416, 485
AcuI CTGAAG 3 cut(s) 74, 560, 914
AfaI GTAC 2 cut(s) 468, 852
AfiI CCNNNNNNNGG 5 cut(s) 278, 638, 676, 841, 940
AgsI TTSAA 6 cut(s) 15, 82, 109, 278, 290, 890
AjnI CCWGG 3 cut(s) 154, 909, 939
Alw21I GWGCWC 4 cut(s) 70, 385, 700, 886
AlwI GGATC 4 cut(s) 369, 663, 839, 852
Aor13HI TCCGGA 1 cut(s) 634
AoxI GGCC 1 cut(s) 397
ApeKI GCWGC 3 cut(s) 149, 582, 808
ApoI RAATTY 2 cut(s) 416, 485
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
AspLEI GCGC 1 cut(s) 132
AspS9I GGNCC 1 cut(s) 734
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 3 cut(s) 311, 782, 910
AvaII GGWCC 1 cut(s) 734
AxyI CCTNAGG 2 cut(s) 651, 691
BamHI GGATCC 1 cut(s) 844
BanII GRGCYC 1 cut(s) 886
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 4 cut(s) 70, 385, 700, 886
BbvI GCAGC 3 cut(s) 136, 594, 795
BccI CCATC 2 cut(s) 341, 371
BceAI ACGGC 1 cut(s) 241
BcgI CGANNNNNNTGC 4 cut(s) 128, 162, 189, 223
BciT130I CCWGG 3 cut(s) 156, 911, 941
BcnI CCSGG 1 cut(s) 197
BfaI CTAG 3 cut(s) 440, 671, 707
BfuAI ACCTGC 1 cut(s) 434
BisI GCNGC 4 cut(s) 150, 583, 586, 809
BlsI GCNGC 4 cut(s) 151, 584, 587, 810
Bme1390I CCNGG 4 cut(s) 156, 197, 911, 941
Bme18I GGWCC 1 cut(s) 734
BmgT120I GGNCC 1 cut(s) 734
BmiI GGNNCC 2 cut(s) 270, 846
BmrFI CCNGG 4 cut(s) 156, 197, 911, 941
BmsI GCATC 2 cut(s) 670, 808
BpiI GAAGAC 1 cut(s) 515
BplI GAGNNNNNCTC 1 cut(s) 920
BpmI CTGGAG 2 cut(s) 184, 369
BpuEI CTTGAG 1 cut(s) 947
BpuMI CCSGG 1 cut(s) 197
BsaBI GATNNNNATC 1 cut(s) 660
BsaJI CCNNGG 3 cut(s) 505, 910, 939
BsaWI WCCGGW 2 cut(s) 634, 834
Bsc4I CCNNNNNNNGG 5 cut(s) 278, 638, 676, 841, 940
Bse1I ACTGG 1 cut(s) 400
Bse21I CCTNAGG 2 cut(s) 651, 691
Bse8I GATNNNNATC 1 cut(s) 660
BseAI TCCGGA 1 cut(s) 634
BseBI CCWGG 3 cut(s) 156, 911, 941
BseDI CCNNGG 3 cut(s) 505, 910, 939
BseGI GGATG 1 cut(s) 418
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 5 cut(s) 278, 638, 676, 841, 940
BseMII CTCAG 2 cut(s) 705, 790
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 3 cut(s) 116, 621, 858
BseXI GCAGC 3 cut(s) 136, 594, 795
BseYI CCCAGC 1 cut(s) 429
BsgI GTGCAG 2 cut(s) 314, 443
Bsh1236I CGCG 1 cut(s) 132
BshFI GGCC 1 cut(s) 399
BsiHKAI GWGCWC 4 cut(s) 70, 385, 700, 886
BsiSI CCGG 3 cut(s) 197, 635, 835
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 5 cut(s) 278, 638, 676, 841, 940
BsmFI GGGAC 1 cut(s) 412
BsnI GGCC 1 cut(s) 399
Bsp1286I GDGCHC 4 cut(s) 70, 385, 700, 886
Bsp13I TCCGGA 1 cut(s) 634
Bsp1407I TGTACA 1 cut(s) 850
Bsp143I GATC 5 cut(s) 139, 361, 655, 703, 844
BspACI CCGC 4 cut(s) 321, 359, 586, 806
BspANI GGCC 1 cut(s) 399
BspCNI CTCAG 2 cut(s) 704, 789
BspEI TCCGGA 1 cut(s) 634
BspFNI CGCG 1 cut(s) 132
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 270, 846
BspMI ACCTGC 1 cut(s) 434
BspPI GGATC 4 cut(s) 369, 663, 839, 852
BsrGI TGTACA 1 cut(s) 850
BsrI ACTGG 1 cut(s) 400
BssECI CCNNGG 3 cut(s) 505, 910, 939
BssMI GATC 5 cut(s) 139, 361, 655, 703, 844
Bst2UI CCWGG 3 cut(s) 156, 911, 941
Bst4CI ACNGT 1 cut(s) 797
BstAUI TGTACA 1 cut(s) 850
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 4 cut(s) 219, 651, 691, 776
BstDSI CCRYGG 1 cut(s) 505
BstEII GGTNACC 1 cut(s) 770
BstENI CCTNNNNNAGG 1 cut(s) 674
BstF5I GGATG 1 cut(s) 418
BstFNI CGCG 1 cut(s) 132
BstHHI GCGC 1 cut(s) 132
BstKTI GATC 5 cut(s) 142, 364, 658, 706, 847
BstMBI GATC 5 cut(s) 139, 361, 655, 703, 844
BstMWI GCNNNNNNNGC 5 cut(s) 74, 320, 430, 594, 827
BstNI CCWGG 3 cut(s) 156, 911, 941
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 4 cut(s) 154, 195, 909, 939
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 136, 594, 795
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 3 cut(s) 361, 655, 844
BstYI RGATCY 3 cut(s) 361, 655, 844
Bsu36I CCTNAGG 2 cut(s) 651, 691
BsuRI GGCC 1 cut(s) 399
BtgI CCRYGG 1 cut(s) 505
BtgZI GCGATG 2 cut(s) 243, 647
BtsCI GGATG 1 cut(s) 418
BtsIMutI CAGTG 1 cut(s) 784
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 1 cut(s) 66
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 132
Cfr13I GGNCC 1 cut(s) 734
CseI GACGC 1 cut(s) 121
Csp6I GTAC 2 cut(s) 467, 851
CspCI CAANNNNNGTGG 2 cut(s) 460, 495
CviAII CATG 4 cut(s) 404, 409, 464, 701
CviQI GTAC 2 cut(s) 467, 851
DdeI CTNAG 4 cut(s) 219, 651, 691, 776
DpnI GATC 5 cut(s) 141, 363, 657, 705, 846
DpnII GATC 5 cut(s) 139, 361, 655, 703, 844
EciI GGCGGA 1 cut(s) 374
Ecl136II GAGCTC 1 cut(s) 884
Eco24I GRGCYC 1 cut(s) 886
Eco32I GATATC 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 734
Eco53kI GAGCTC 1 cut(s) 884
Eco57I CTGAAG 3 cut(s) 74, 560, 914
Eco81I CCTNAGG 2 cut(s) 651, 691
Eco91I GGTNACC 1 cut(s) 770
EcoICRI GAGCTC 1 cut(s) 884
EcoNI CCTNNNNNAGG 1 cut(s) 674
EcoO65I GGTNACC 1 cut(s) 770
EcoRII CCWGG 3 cut(s) 154, 909, 939
EcoRV GATATC 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 886
FaeI CATG 4 cut(s) 407, 412, 467, 704
FaqI GGGAC 1 cut(s) 412
FatI CATG 4 cut(s) 403, 408, 463, 700
Fnu4HI GCNGC 4 cut(s) 150, 583, 586, 809
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 1 cut(s) 886
Fsp4HI GCNGC 4 cut(s) 150, 583, 586, 809
FspBI CTAG 3 cut(s) 440, 671, 707
GlaI GCGC 1 cut(s) 131
GluI GCNGC 4 cut(s) 150, 583, 586, 809
GsaI CCCAGC 1 cut(s) 433
GsuI CTGGAG 2 cut(s) 184, 369
HaeIII GGCC 1 cut(s) 399
HapII CCGG 3 cut(s) 197, 635, 835
HgaI GACGC 1 cut(s) 121
HhaI GCGC 1 cut(s) 132
Hin1II CATG 4 cut(s) 407, 412, 467, 704
Hin6I GCGC 1 cut(s) 130
HinP1I GCGC 1 cut(s) 130
HindIII AAGCTT 4 cut(s) 9, 83, 203, 900
HinfI GANTC 7 cut(s) 123, 166, 215, 642, 788, 915, 944
HpaII CCGG 3 cut(s) 197, 635, 835
HphI GGTGA 3 cut(s) 311, 782, 910
Hpy188I TCNGA 6 cut(s) 72, 93, 214, 273, 338, 793
Hpy188III TCNNGA 8 cut(s) 386, 404, 553, 635, 693, 707, 887, 948
Hpy99I CGWCG 3 cut(s) 122, 137, 140
HpyAV CCTTC 7 cut(s) 50, 88, 272, 410, 470, 535, 725
HpyCH4III ACNGT 1 cut(s) 797
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 9 cut(s) 295, 314, 354, 424, 494, 569, 611, 661, 714
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 320, 430, 594, 827
HpyF3I CTNAG 4 cut(s) 219, 651, 691, 776
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 4 cut(s) 407, 412, 467, 704
HspAI GCGC 1 cut(s) 130
Kpn2I TCCGGA 1 cut(s) 634
Kzo9I GATC 5 cut(s) 139, 361, 655, 703, 844
LmnI GCTCC 7 cut(s) 157, 274, 388, 695, 827, 837, 871
Lsp1109I GCAGC 3 cut(s) 136, 594, 795
LweI GCATC 2 cut(s) 670, 808
MaeI CTAG 3 cut(s) 440, 671, 707
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 4 cut(s) 391, 457, 559, 770
MalI GATC 5 cut(s) 141, 363, 657, 705, 846
MboI GATC 5 cut(s) 139, 361, 655, 703, 844
MboII GAAGA 5 cut(s) 65, 80, 106, 515, 907
MfeI CAATTG 2 cut(s) 453, 861
MflI RGATCY 3 cut(s) 361, 655, 844
MhlI GDGCHC 4 cut(s) 70, 385, 700, 886
MluCI AATT 7 cut(s) 185, 416, 453, 485, 589, 622, 861
MlyI GAGTC 2 cut(s) 132, 175
MmeI TCCRAC 1 cut(s) 708
MroI TCCGGA 1 cut(s) 634
MseI TTAA 1 cut(s) 449
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 3 cut(s) 433, 538, 808
MspI CCGG 3 cut(s) 197, 635, 835
MspR9I CCNGG 4 cut(s) 156, 197, 911, 941
MunI CAATTG 2 cut(s) 453, 861
MvaI CCWGG 3 cut(s) 156, 911, 941
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 5 cut(s) 74, 320, 430, 594, 827
NciI CCSGG 1 cut(s) 197
NdeII GATC 5 cut(s) 139, 361, 655, 703, 844
NlaIII CATG 4 cut(s) 407, 412, 467, 704
NlaIV GGNNCC 2 cut(s) 270, 846
NmuCI GTSAC 2 cut(s) 559, 770
PagI TCATGA 1 cut(s) 403
PfeI GAWTC 5 cut(s) 215, 642, 788, 915, 944
PflFI GACNNNGTC 1 cut(s) 122
PflMI CCANNNNNTGG 1 cut(s) 940
PkrI GCNGC 4 cut(s) 151, 584, 587, 810
PleI GAGTC 2 cut(s) 131, 174
PpsI GAGTC 2 cut(s) 131, 174
PsiI TTATAA 1 cut(s) 876
Psp124BI GAGCTC 1 cut(s) 886
Psp6I CCWGG 3 cut(s) 154, 909, 939
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 1 cut(s) 429
PspGI CCWGG 3 cut(s) 154, 909, 939
PspN4I GGNNCC 2 cut(s) 270, 846
PspPI GGNCC 1 cut(s) 734
PsuI RGATCY 3 cut(s) 361, 655, 844
PsyI GACNNNGTC 1 cut(s) 122
PvuII CAGCTG 2 cut(s) 433, 538
RsaI GTAC 2 cut(s) 468, 852
RsaNI GTAC 2 cut(s) 467, 851
RseI CAYNNNNRTG 1 cut(s) 345
SacI GAGCTC 1 cut(s) 886
SaqAI TTAA 1 cut(s) 449
SatI GCNGC 4 cut(s) 150, 583, 586, 809
Sau3AI GATC 5 cut(s) 139, 361, 655, 703, 844
Sau96I GGNCC 1 cut(s) 734
SchI GAGTC 2 cut(s) 132, 175
ScrFI CCNGG 4 cut(s) 156, 197, 911, 941
SduI GDGCHC 4 cut(s) 70, 385, 700, 886
SfaNI GCATC 2 cut(s) 670, 808
SinI GGWCC 1 cut(s) 734
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
Sse9I AATT 7 cut(s) 185, 416, 453, 485, 589, 622, 861
SsiI CCGC 4 cut(s) 321, 359, 586, 806
SspMI CTAG 3 cut(s) 440, 671, 707
SstI GAGCTC 1 cut(s) 886
StyD4I CCNGG 4 cut(s) 154, 195, 909, 939
TaaI ACNGT 1 cut(s) 797
TaiI ACGT 1 cut(s) 393
TaqI TCGA 3 cut(s) 117, 138, 645
TasI AATT 7 cut(s) 185, 416, 453, 485, 589, 622, 861
TatI WGTACW 2 cut(s) 466, 850
TauI GCSGC 1 cut(s) 588
TfiI GAWTC 5 cut(s) 215, 642, 788, 915, 944
Tru1I TTAA 1 cut(s) 449
Tru9I TTAA 1 cut(s) 449
TscAI CASTG 1 cut(s) 784
TseFI GTSAC 2 cut(s) 559, 770
TseI GCWGC 3 cut(s) 149, 582, 808
Tsp45I GTSAC 2 cut(s) 559, 770
TspDTI ATGAA 3 cut(s) 392, 429, 789
TspGWI ACGGA 2 cut(s) 135, 494
TspRI CASTG 1 cut(s) 784
Tth111I GACNNNGTC 1 cut(s) 122
Van91I CCANNNNNTGG 1 cut(s) 940
VpaK11BI GGWCC 1 cut(s) 734
XagI CCTNNNNNAGG 1 cut(s) 674
XapI RAATTY 2 cut(s) 416, 485
XbaI TCTAGA 1 cut(s) 706
XspI CTAG 3 cut(s) 440, 671, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.