AT5G01740

Wound-induced protein WI12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015133
Physical Location & Seq
Forward (+)
280674 .. 281532
859 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01740.1

Sequence Viewer

Length: 489 bp
ATGGAAGCACACAACGTAGAGATATCAACAAGACTTACCCCAGAAATGAAAAACCAGGGAACCATAGAGTTACTCTACAAAGCGTTGCAGCAAGGCGACACTTCTACGGTGACCAAAGTGGTGGCCAGTGACGTGGAATGGTGGTTCCATGGACCGCACGATTGTCAACACATGATGAGGCTGCTAACAGGGGAACCTCCGAGGCAGGTTTCGTTCAGGTTTGAGCCAAGCAGTGTACAAGTAGTGGTGCCTGGTCATGACTGCGTGATTGCAGAAGGGTGGGAGGGTTCACGCGTGTACTGGGTTCACGTGTGGAAATTGAAAGACGGAGTGGTCACTGAGCTAAGAGAGTATTTCAACACATGGCTCACTGTTACAGACTACAGTCTTGGAGCAGTAGGATGGGACATGGGGCGTTGTACGGTTTGGGAGAGCGTACCAAGGGACCTGGCTCGTGGTTCGTTGCCTAGCTTGTTGTTAGCTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WI12

Protein Analysis

162

Amino Acids

18.43

Weight (kDa)

5.45

Isoelectric Point (pI)

24.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WI12 PF07107 59 - 162 4.2e-46 Wound-induced protein WI12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01740
fragaria_vesca FvH4_6g10320
malus_domestica MD04G1168600.v1.1 MD12G1182200.v1.1
prunus_persica Prupe.6G286900_v2.0.a1
pyrus_communis pycom12g17130
rosa_chinensis RchiOBHm_Chr3g0461091
rosa_laevigata RLG00000024953
rosa_multiflora Rmu_co8398679.1_g000001 Rmu_sc0001521.1_g000057
rosa_roxburghii Rroxscaffold_6G00418860
rosa_rugosa Rorug03G0048100
rosa_samantha Rh3BG109800 Rh3CG111300 Rh3DG111500
rosa_wichuraiana Rw3G009040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 332
Acc36I ACCTGC 1 cut(s) 196
AccB1I GGYRCC 1 cut(s) 247
AccII CGCG 1 cut(s) 294
AciI CCGC 1 cut(s) 155
AcoI YGGCCR 1 cut(s) 123
AcvI CACGTG 1 cut(s) 310
AfaI GTAC 4 cut(s) 237, 299, 421, 438
AflIII ACRYGT 2 cut(s) 292, 309
AgsI TTSAA 2 cut(s) 322, 358
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 3 cut(s) 54, 250, 447
AloI GAACNNNNNNTCC 2 cut(s) 128, 160
AluBI AGCT 3 cut(s) 343, 471, 482
AluI AGCT 3 cut(s) 343, 471, 482
AoxI GGCC 1 cut(s) 123
ApeKI GCWGC 2 cut(s) 88, 181
AspS9I GGNCC 2 cut(s) 152, 445
AsuHPI GGTGA 1 cut(s) 121
AvaII GGWCC 2 cut(s) 152, 445
BalI TGGCCA 1 cut(s) 125
BanI GGYRCC 1 cut(s) 247
BauI CACGAG 1 cut(s) 453
BbrPI CACGTG 1 cut(s) 310
BbvI GCAGC 2 cut(s) 100, 168
BccI CCATC 1 cut(s) 396
BciT130I CCWGG 3 cut(s) 56, 252, 449
BfaI CTAG 1 cut(s) 468
BfmI CTRYAG 1 cut(s) 382
BfuAI ACCTGC 1 cut(s) 196
BisI GCNGC 2 cut(s) 89, 182
BlsI GCNGC 2 cut(s) 90, 183
Bme1390I CCNGG 3 cut(s) 56, 252, 449
Bme18I GGWCC 2 cut(s) 152, 445
BmgBI CACGTC 1 cut(s) 133
BmgT120I GGNCC 2 cut(s) 152, 445
BmiI GGNNCC 5 cut(s) 61, 146, 195, 249, 446
BmrFI CCNGG 3 cut(s) 56, 252, 449
BmrI ACTGGG 1 cut(s) 310
BmuI ACTGGG 1 cut(s) 310
BoxI GACNNNNGTC 1 cut(s) 384
BsaAI YACGTR 1 cut(s) 310
BsaJI CCNNGG 4 cut(s) 55, 148, 200, 440
Bse1I ACTGG 2 cut(s) 126, 305
BseBI CCWGG 3 cut(s) 56, 252, 449
BseDI CCNNGG 4 cut(s) 55, 148, 200, 440
BseGI GGATG 1 cut(s) 407
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 2 cut(s) 126, 305
BseXI GCAGC 2 cut(s) 100, 168
Bsh1236I CGCG 1 cut(s) 294
BshFI GGCC 1 cut(s) 125
BshNI GGYRCC 1 cut(s) 247
BslFI GGGAC 2 cut(s) 419, 458
BsmFI GGGAC 2 cut(s) 419, 458
BsnI GGCC 1 cut(s) 125
Bsp1407I TGTACA 1 cut(s) 235
Bsp19I CCATGG 1 cut(s) 148
BspACI CCGC 1 cut(s) 155
BspANI GGCC 1 cut(s) 125
BspCNI CTCAG 1 cut(s) 331
BspFNI CGCG 1 cut(s) 294
BspHI TCATGA 1 cut(s) 256
BspLI GGNNCC 5 cut(s) 61, 146, 195, 249, 446
BspMI ACCTGC 1 cut(s) 196
BspT107I GGYRCC 1 cut(s) 247
BsrGI TGTACA 1 cut(s) 235
BsrI ACTGG 2 cut(s) 126, 305
BssECI CCNNGG 4 cut(s) 55, 148, 200, 440
BssSI CACGAG 1 cut(s) 453
BssT1I CCWWGG 2 cut(s) 148, 440
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 3 cut(s) 56, 252, 449
Bst4CI ACNGT 4 cut(s) 109, 373, 386, 424
BstAUI TGTACA 1 cut(s) 235
BstBAI YACGTR 1 cut(s) 310
BstDEI CTNAG 2 cut(s) 339, 344
BstDSI CCRYGG 1 cut(s) 148
BstEII GGTNACC 1 cut(s) 109
BstF5I GGATG 1 cut(s) 407
BstFNI CGCG 1 cut(s) 294
BstNI CCWGG 3 cut(s) 56, 252, 449
BstPAI GACNNNNGTC 1 cut(s) 384
BstPI GGTNACC 1 cut(s) 109
BstSCI CCNGG 3 cut(s) 54, 250, 447
BstSFI CTRYAG 1 cut(s) 382
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 2 cut(s) 100, 168
BstXI CCANNNNNNTGG 2 cut(s) 121, 133
BsuRI GGCC 1 cut(s) 125
BtgI CCRYGG 1 cut(s) 148
BtrI CACGTC 1 cut(s) 133
BtsCI GGATG 1 cut(s) 407
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 4 cut(s) 133, 238, 336, 369
BveI ACCTGC 1 cut(s) 196
CciI TCATGA 1 cut(s) 256
Cfr13I GGNCC 2 cut(s) 152, 445
Csp6I GTAC 4 cut(s) 236, 298, 420, 437
CviAII CATG 5 cut(s) 149, 172, 257, 363, 409
CviJI RGCY 8 cut(s) 125, 181, 226, 343, 367, 452, 471, 482
CviKI_1 RGCY 8 cut(s) 125, 181, 226, 343, 367, 452, 471, 482
CviQI GTAC 4 cut(s) 236, 298, 420, 437
DdeI CTNAG 2 cut(s) 339, 344
DrdI GACNNNNNNGTC 1 cut(s) 332
DseDI GACNNNNNNGTC 1 cut(s) 332
EaeI YGGCCR 1 cut(s) 123
Eco130I CCWWGG 2 cut(s) 148, 440
Eco32I GATATC 1 cut(s) 24
Eco47I GGWCC 2 cut(s) 152, 445
Eco72I CACGTG 1 cut(s) 310
Eco91I GGTNACC 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 445
EcoO65I GGTNACC 1 cut(s) 109
EcoRII CCWGG 3 cut(s) 54, 250, 447
EcoRV GATATC 1 cut(s) 24
EcoT14I CCWWGG 2 cut(s) 148, 440
ErhI CCWWGG 2 cut(s) 148, 440
FaeI CATG 5 cut(s) 152, 175, 260, 366, 412
FaiI YATR 6 cut(s) 65, 150, 173, 258, 364, 410
FaqI GGGAC 2 cut(s) 419, 458
FatI CATG 5 cut(s) 148, 171, 256, 362, 408
Fnu4HI GCNGC 2 cut(s) 89, 182
FokI GGATG 1 cut(s) 414
Fsp4HI GCNGC 2 cut(s) 89, 182
FspBI CTAG 1 cut(s) 468
GluI GCNGC 2 cut(s) 89, 182
HaeIII GGCC 1 cut(s) 125
Hin1II CATG 5 cut(s) 152, 175, 260, 366, 412
HincII GTYRAC 1 cut(s) 167
HindII GTYRAC 1 cut(s) 167
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 5 cut(s) 167, 236, 290, 298, 307
Hpy188I TCNGA 1 cut(s) 201
Hpy188III TCNNGA 1 cut(s) 257
Hpy8I GTNNAC 5 cut(s) 167, 236, 290, 298, 307
HpyAV CCTTC 1 cut(s) 269
HpyCH4III ACNGT 4 cut(s) 109, 373, 386, 424
HpyCH4IV ACGT 3 cut(s) 15, 132, 309
HpyCH4V TGCA 2 cut(s) 88, 272
HpyF3I CTNAG 2 cut(s) 339, 344
HpySE526I ACGT 3 cut(s) 15, 132, 309
Hsp92II CATG 5 cut(s) 152, 175, 260, 366, 412
LmnI GCTCC 1 cut(s) 392
Lsp1109I GCAGC 2 cut(s) 100, 168
MaeI CTAG 1 cut(s) 468
MaeII ACGT 3 cut(s) 15, 132, 309
MaeIII GTNAC 5 cut(s) 69, 109, 128, 334, 373
MlsI TGGCCA 1 cut(s) 125
MluCI AATT 1 cut(s) 317
MluI ACGCGT 1 cut(s) 292
MluNI TGGCCA 1 cut(s) 125
MnlI CCTC 4 cut(s) 171, 195, 207, 277
Mox20I TGGCCA 1 cut(s) 125
MscI TGGCCA 1 cut(s) 125
MseI TTAA 1 cut(s) 487
Msp20I TGGCCA 1 cut(s) 125
MspR9I CCNGG 3 cut(s) 56, 252, 449
MvaI CCWGG 3 cut(s) 56, 252, 449
MvnI CGCG 1 cut(s) 294
NcoI CCATGG 1 cut(s) 148
NlaIII CATG 5 cut(s) 152, 175, 260, 366, 412
NlaIV GGNNCC 5 cut(s) 61, 146, 195, 249, 446
NmuCI GTSAC 3 cut(s) 109, 128, 334
PagI TCATGA 1 cut(s) 256
PkrI GCNGC 2 cut(s) 90, 183
PmaCI CACGTG 1 cut(s) 310
PmlI CACGTG 1 cut(s) 310
Ppu21I YACGTR 1 cut(s) 310
PpuMI RGGWCCY 1 cut(s) 445
PshAI GACNNNNGTC 1 cut(s) 384
Psp5II RGGWCCY 1 cut(s) 445
Psp6I CCWGG 3 cut(s) 54, 250, 447
PspCI CACGTG 1 cut(s) 310
PspEI GGTNACC 1 cut(s) 109
PspGI CCWGG 3 cut(s) 54, 250, 447
PspN4I GGNNCC 5 cut(s) 61, 146, 195, 249, 446
PspPI GGNCC 2 cut(s) 152, 445
PspPPI RGGWCCY 1 cut(s) 445
RsaI GTAC 4 cut(s) 237, 299, 421, 438
RsaNI GTAC 4 cut(s) 236, 298, 420, 437
SaqAI TTAA 1 cut(s) 487
SatI GCNGC 2 cut(s) 89, 182
Sau96I GGNCC 2 cut(s) 152, 445
ScrFI CCNGG 3 cut(s) 56, 252, 449
SfcI CTRYAG 1 cut(s) 382
SinI GGWCC 2 cut(s) 152, 445
Sse9I AATT 1 cut(s) 317
SsiI CCGC 1 cut(s) 155
SspMI CTAG 1 cut(s) 468
StyD4I CCNGG 3 cut(s) 54, 250, 447
StyI CCWWGG 2 cut(s) 148, 440
TaaI ACNGT 4 cut(s) 109, 373, 386, 424
TaiI ACGT 3 cut(s) 18, 135, 312
TasI AATT 1 cut(s) 317
TatI WGTACW 2 cut(s) 235, 297
Tru1I TTAA 1 cut(s) 487
Tru9I TTAA 1 cut(s) 487
TscAI CASTG 4 cut(s) 133, 238, 343, 376
TseFI GTSAC 3 cut(s) 109, 128, 334
TseI GCWGC 2 cut(s) 88, 181
Tsp45I GTSAC 3 cut(s) 109, 128, 334
TspDTI ATGAA 1 cut(s) 62
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 4 cut(s) 133, 238, 343, 376
VpaK11BI GGWCC 2 cut(s) 152, 445
XspI CTAG 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.