MD12G1182200.v1.1

Wound-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015133
Physical Location & Seq
Forward (+)
26205440 .. 26205862
423 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1182200.v1.1.491

Sequence Viewer

Length: 423 bp
ATGCAAAACAAGGCAACCGTTGAAGCTCTCTACAAGGCGTTGGCACAGAGCCAAACAGACACAGTGGGAAAGCTTCTAGCCAGTGACCTAGAATGGTGGTTCCATGGCCCCCCAAAGAGCCAGCATATGATGCACGTGCTCACGGGGGATTCCAGCCACACTGACTTCAGGTTCGAGCCCAGAAGCATCACAGAGATTGGTGGTGGTGTGATCATTGCTGAGGGATGGGAAGGGGCAAAGGCCTACTGGGTCCACGTGTGGACCCTCAAAGATGGGTTGATCACTCAGTTCAGGGAGTATTTTAACACGTGGCTGACTGTAAAGGATCTGAAGCCGGAGGGATGGGAGAGCTTCACTGTGTGGCAGAGCATGCCGAGGGACCTGTTCCACCGCTCCTTGCCAACCCTATTGCTTGCTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WI12

Protein Analysis

141

Amino Acids

16.11

Weight (kDa)

6.04

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WI12 PF07107 44 - 140 8.7e-30 Wound-induced protein WI12
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01740
fragaria_vesca FvH4_6g10320
malus_domestica MD04G1168600.v1.1 MD12G1182200.v1.1
prunus_persica Prupe.6G286900_v2.0.a1
pyrus_communis pycom12g17130
rosa_chinensis RchiOBHm_Chr3g0461091
rosa_laevigata RLG00000024953
rosa_multiflora Rmu_co8398679.1_g000001 Rmu_sc0001521.1_g000057
rosa_roxburghii Rroxscaffold_6G00418860
rosa_rugosa Rorug03G0048100
rosa_samantha Rh3BG109800 Rh3CG111300 Rh3DG111500
rosa_wichuraiana Rw3G009040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 393
AciI CCGC 1 cut(s) 391
AclWI GGATC 1 cut(s) 333
AcuI CTGAAG 2 cut(s) 151, 350
AcvI CACGTG 3 cut(s) 136, 256, 309
AdeI CACNNNGTG 1 cut(s) 360
AflIII ACRYGT 2 cut(s) 255, 306
AgsI TTSAA 1 cut(s) 23
AluBI AGCT 3 cut(s) 26, 73, 351
AluI AGCT 3 cut(s) 26, 73, 351
Alw21I GWGCWC 1 cut(s) 141
AlwI GGATC 1 cut(s) 333
AoxI GGCC 2 cut(s) 106, 240
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AspS9I GGNCC 4 cut(s) 107, 250, 261, 379
AvaII GGWCC 3 cut(s) 250, 261, 379
BanII GRGCYC 1 cut(s) 180
BbrPI CACGTG 3 cut(s) 136, 256, 309
Bbv12I GWGCWC 1 cut(s) 141
BbvCI CCTCAGC 1 cut(s) 219
BccI CCATC 3 cut(s) 219, 266, 336
BclI TGATCA 2 cut(s) 210, 279
BfaI CTAG 2 cut(s) 77, 89
Bme18I GGWCC 3 cut(s) 250, 261, 379
BmgT120I GGNCC 4 cut(s) 107, 250, 261, 379
BmiI GGNNCC 5 cut(s) 101, 109, 251, 263, 380
BmrI ACTGGG 1 cut(s) 256
BmsI GCATC 2 cut(s) 120, 195
BmuI ACTGGG 1 cut(s) 256
Bpu10I CCTNAGC 1 cut(s) 219
BsaAI YACGTR 3 cut(s) 136, 256, 309
BsaJI CCNNGG 2 cut(s) 103, 374
Bse1I ACTGG 2 cut(s) 81, 251
Bse3DI GCAATG 1 cut(s) 213
BseDI CCNNGG 2 cut(s) 103, 374
BseGI GGATG 2 cut(s) 230, 347
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 2 cut(s) 210, 299
BseNI ACTGG 2 cut(s) 81, 251
BshFI GGCC 2 cut(s) 108, 242
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 1 cut(s) 335
BslFI GGGAC 1 cut(s) 392
BsmFI GGGAC 1 cut(s) 392
BsnI GGCC 2 cut(s) 108, 242
Bsp1286I GDGCHC 2 cut(s) 141, 180
Bsp143I GATC 3 cut(s) 210, 279, 325
Bsp19I CCATGG 1 cut(s) 103
BspACI CCGC 1 cut(s) 391
BspANI GGCC 2 cut(s) 108, 242
BspCNI CTCAG 2 cut(s) 211, 298
BspLI GGNNCC 5 cut(s) 101, 109, 251, 263, 380
BspPI GGATC 1 cut(s) 333
BsrBI CCGCTC 1 cut(s) 393
BsrDI GCAATG 1 cut(s) 213
BsrI ACTGG 2 cut(s) 81, 251
BssECI CCNNGG 2 cut(s) 103, 374
BssMI GATC 3 cut(s) 210, 279, 325
BssT1I CCWWGG 1 cut(s) 103
Bst4CI ACNGT 4 cut(s) 19, 64, 319, 358
BstAPI GCANNNNNTGC 2 cut(s) 130, 370
BstBAI YACGTR 3 cut(s) 136, 256, 309
BstC8I GCNNGC 3 cut(s) 122, 371, 414
BstDEI CTNAG 2 cut(s) 219, 285
BstDSI CCRYGG 1 cut(s) 103
BstF5I GGATG 2 cut(s) 230, 347
BstKTI GATC 3 cut(s) 213, 282, 328
BstMBI GATC 3 cut(s) 210, 279, 325
BstMWI GCNNNNNNNGC 2 cut(s) 130, 370
BstNSI RCATGY 1 cut(s) 373
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
BsuRI GGCC 2 cut(s) 108, 242
BtgI CCRYGG 1 cut(s) 103
BtsCI GGATG 2 cut(s) 230, 347
BtsIMutI CAGTG 4 cut(s) 69, 88, 159, 354
Cac8I GCNNGC 3 cut(s) 122, 371, 414
Cfr13I GGNCC 4 cut(s) 107, 250, 261, 379
CviAII CATG 2 cut(s) 104, 370
DdeI CTNAG 2 cut(s) 219, 285
DpnI GATC 3 cut(s) 212, 281, 327
DpnII GATC 3 cut(s) 210, 279, 325
DraIII CACNNNGTG 1 cut(s) 360
Eco130I CCWWGG 1 cut(s) 103
Eco147I AGGCCT 1 cut(s) 242
Eco24I GRGCYC 1 cut(s) 180
Eco47I GGWCC 3 cut(s) 250, 261, 379
Eco57I CTGAAG 2 cut(s) 151, 350
Eco72I CACGTG 3 cut(s) 136, 256, 309
EcoO109I RGGNCCY 1 cut(s) 379
EcoT14I CCWWGG 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 103
FaeI CATG 2 cut(s) 107, 373
FaiI YATR 4 cut(s) 105, 126, 128, 371
FaqI GGGAC 1 cut(s) 392
FatI CATG 2 cut(s) 103, 369
FauNDI CATATG 1 cut(s) 126
FbaI TGATCA 2 cut(s) 210, 279
FokI GGATG 2 cut(s) 237, 354
FriOI GRGCYC 1 cut(s) 180
FspBI CTAG 2 cut(s) 77, 89
HaeIII GGCC 2 cut(s) 108, 242
HapII CCGG 1 cut(s) 335
Hin1II CATG 2 cut(s) 107, 373
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 1 cut(s) 149
HpaII CCGG 1 cut(s) 335
Hpy166II GTNNAC 2 cut(s) 253, 261
Hpy188I TCNGA 1 cut(s) 330
Hpy8I GTNNAC 2 cut(s) 253, 261
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 4 cut(s) 19, 64, 319, 358
HpyCH4IV ACGT 3 cut(s) 135, 255, 308
HpyCH4V TGCA 2 cut(s) 4, 133
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 370
HpyF3I CTNAG 2 cut(s) 219, 285
HpySE526I ACGT 3 cut(s) 135, 255, 308
Hsp92II CATG 2 cut(s) 107, 373
Ksp22I TGATCA 2 cut(s) 210, 279
Kzo9I GATC 3 cut(s) 210, 279, 325
LmnI GCTCC 1 cut(s) 398
LpnPI CCDG 9 cut(s) 94, 134, 154, 166, 193, 232, 277, 348, 395
LweI GCATC 2 cut(s) 120, 195
MaeI CTAG 2 cut(s) 77, 89
MaeII ACGT 3 cut(s) 135, 255, 308
MaeIII GTNAC 1 cut(s) 83
MalI GATC 3 cut(s) 212, 281, 327
MbiI CCGCTC 1 cut(s) 393
MboI GATC 3 cut(s) 210, 279, 325
MflI RGATCY 1 cut(s) 325
MhlI GDGCHC 2 cut(s) 141, 180
MnlI CCTC 4 cut(s) 214, 275, 331, 369
MseI TTAA 1 cut(s) 303
MspI CCGG 1 cut(s) 335
MwoI GCNNNNNNNGC 2 cut(s) 130, 370
NcoI CCATGG 1 cut(s) 103
NdeI CATATG 1 cut(s) 126
NdeII GATC 3 cut(s) 210, 279, 325
NlaIII CATG 2 cut(s) 107, 373
NlaIV GGNNCC 5 cut(s) 101, 109, 251, 263, 380
NmeAIII GCCGAG 1 cut(s) 399
NmuCI GTSAC 1 cut(s) 83
NspI RCATGY 1 cut(s) 373
PaeI GCATGC 1 cut(s) 373
PceI AGGCCT 1 cut(s) 242
PfeI GAWTC 1 cut(s) 149
PmaCI CACGTG 3 cut(s) 136, 256, 309
PmlI CACGTG 3 cut(s) 136, 256, 309
Ppu21I YACGTR 3 cut(s) 136, 256, 309
PpuMI RGGWCCY 1 cut(s) 379
Psp5II RGGWCCY 1 cut(s) 379
PspCI CACGTG 3 cut(s) 136, 256, 309
PspN4I GGNNCC 5 cut(s) 101, 109, 251, 263, 380
PspPI GGNCC 4 cut(s) 107, 250, 261, 379
PspPPI RGGWCCY 1 cut(s) 379
PsuI RGATCY 1 cut(s) 325
SaqAI TTAA 1 cut(s) 303
Sau3AI GATC 3 cut(s) 210, 279, 325
Sau96I GGNCC 4 cut(s) 107, 250, 261, 379
SduI GDGCHC 2 cut(s) 141, 180
SetI ASST 9 cut(s) 28, 75, 90, 138, 173, 258, 311, 353, 384
SfaNI GCATC 2 cut(s) 120, 195
SinI GGWCC 3 cut(s) 250, 261, 379
SphI GCATGC 1 cut(s) 373
SseBI AGGCCT 1 cut(s) 242
SsiI CCGC 1 cut(s) 391
SspMI CTAG 2 cut(s) 77, 89
StuI AGGCCT 1 cut(s) 242
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 4 cut(s) 19, 64, 319, 358
TaiI ACGT 3 cut(s) 138, 258, 311
TaqI TCGA 1 cut(s) 174
TfiI GAWTC 1 cut(s) 149
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 4 cut(s) 69, 88, 166, 361
TseFI GTSAC 1 cut(s) 83
Tsp45I GTSAC 1 cut(s) 83
TspRI CASTG 4 cut(s) 69, 88, 166, 361
VpaK11BI GGWCC 3 cut(s) 250, 261, 379
XceI RCATGY 1 cut(s) 373
XspI CTAG 2 cut(s) 77, 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.