AT5G02230

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011735
Physical Location & Seq
Forward (+)
448092 .. 450737
2646 bp
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UTR
Exon/CDS
Intron
AT5G02230.1

Sequence Viewer

Length: 843 bp
ATGGAGTTCGAGAACCGGTACGGACTTGCAACGTCGCAGAAGTACGACTGTCTCTTGTTCGATTTGGACGATACTCTTTATCCTCTTAGCTCCGGAATCGCAAGAGAATGTGGGAACAACATCAAAGATTACATGACCGAGAAACTCGGTATACCTAAGGACAAAATCGTCGAATTGTCTGATCTTCTATACAAGAACTATGGAACAACAATGGCTGGTCTTAGGGCGATCGGATATGAATTTGACTACGATGAGTACCACAGCTTCGTCCACGGTCGTTTACCCTATGACAACATAAAACCCGACCTTGTTCTTCGCAGCCTCTTGCTTAGCCTCCCTCTCCGCAAAGTCATCTTTACCAACGCGGACAGAGTTCACGCGGCCAAGGCGTTGAAGAAGTTGGGATTAGAGGATTGTTTCGAAGGAATCATATGTTTCGAAACGCTAAACCTCATGCACACAAACGCTGCGTCTAACAATTCCGAGATTTTTGATATCGTTGGACATTTTAACCGGTCTGAACCGGTCGGTTCACTTCCCAAAACGCCTGTTGTGTGTAAACCATCTGAATCTGCTATCGAGAAAGCTCTTGAAATCGCCAATATTGATCCTAACCGAACGCTGTTCTTTGAGGACAGTGTCCGAAACGTACAAGCTGGAAAACGCGTTGGTCTTTACACGGTTCTGGTTGGTAAATCGACCAAAGTCAAAGGAGCGGATTACGCGTTGGAAAACATTCACAACATGAAAGAGGCGATACCGGAGTTATGGGAATCCGACAGGAAATCGTCGGATGTTGGATATTCCGGCAAGGTCGCCGTTGAGACTTCTGTTAGAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 map00760
Pfam Domains
Protein Families
HAD_2 Hydrolase_like

Protein Analysis

280

Amino Acids

31.34

Weight (kDa)

5.62

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 15 - 224 5.8e-12 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011735)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02230 AT5G02230 AT5G02230
fragaria_vesca FvH4_6g13490 FvH4_6g13490
malus_domestica MD04G1130800.v1.1 MD12G1143900.v1.1
prunus_persica Prupe.6G257300_v2.0.a1 Prupe.6G257300_v2.0.a1 Prupe.6G257300_v2.0.a1
pyrus_communis pycom12g13850
rosa_chinensis RchiOBHm_Chr3g0466361
rosa_laevigata RLG00000024581
rosa_multiflora Rmu_sc0003480.1_g000006
rosa_roxburghii Rroxscaffold_6G00414540
rosa_rugosa Rorug03G0081300
rosa_samantha Rh3AG127700 Rh3DG149700
rosa_wichuraiana Rw3G012060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
AccBSI CCGCTC 1 cut(s) 716
AccI GTMKAC 1 cut(s) 151
AccII CGCG 4 cut(s) 365, 380, 666, 725
AccIII TCCGGA 1 cut(s) 92
AciI CCGC 4 cut(s) 343, 365, 380, 716
AclWI GGATC 1 cut(s) 602
AcoI YGGCCR 1 cut(s) 381
AcsI RAATTY 1 cut(s) 239
AfaI GTAC 4 cut(s) 20, 44, 257, 651
AflIII ACRYGT 2 cut(s) 664, 723
AgeI ACCGGT 3 cut(s) 15, 513, 523
AgsI TTSAA 2 cut(s) 394, 593
AluBI AGCT 5 cut(s) 90, 264, 587, 656, 839
AluI AGCT 5 cut(s) 90, 264, 587, 656, 839
Alw26I GTCTC 2 cut(s) 56, 818
AlwI GGATC 1 cut(s) 602
Aor13HI TCCGGA 1 cut(s) 92
AoxI GGCC 1 cut(s) 381
ApeKI GCWGC 2 cut(s) 318, 467
ApoI RAATTY 1 cut(s) 239
ArsI GACNNNNNNTTYG 2 cut(s) 455, 487
AsiGI ACCGGT 3 cut(s) 15, 513, 523
Asp700I GAANNNNTTC 1 cut(s) 735
AsuII TTCGAA 2 cut(s) 420, 438
AxyI CCTNAGG 1 cut(s) 156
BaeI ACNNNNGTAYC 2 cut(s) 749, 782
BarI GAAGNNNNNNTAC 2 cut(s) 248, 280
BbvI GCAGC 2 cut(s) 330, 454
BccI CCATC 1 cut(s) 571
BceAI ACGGC 1 cut(s) 803
BcoDI GTCTC 2 cut(s) 56, 818
BisI GCNGC 3 cut(s) 319, 381, 468
BlpI GCTNAGC 1 cut(s) 329
BlsI GCNGC 3 cut(s) 320, 382, 469
BoxI GACNNNNGTC 1 cut(s) 704
Bpu1102I GCTNAGC 1 cut(s) 329
Bpu14I TTCGAA 2 cut(s) 420, 438
BsaJI CCNNGG 2 cut(s) 271, 384
BsaWI WCCGGW 5 cut(s) 15, 92, 513, 523, 760
Bse118I RCCGGY 3 cut(s) 15, 513, 523
Bse21I CCTNAGG 1 cut(s) 156
BseAI TCCGGA 1 cut(s) 92
BseDI CCNNGG 2 cut(s) 271, 384
BseGI GGATG 1 cut(s) 799
BseXI GCAGC 2 cut(s) 330, 454
Bsh1236I CGCG 4 cut(s) 365, 380, 666, 725
Bsh1285I CGRYCG 3 cut(s) 231, 277, 528
BshFI GGCC 1 cut(s) 383
BshTI ACCGGT 3 cut(s) 15, 513, 523
BsiEI CGRYCG 3 cut(s) 231, 277, 528
BsiSI CCGG 6 cut(s) 16, 93, 514, 524, 761, 807
BsmAI GTCTC 2 cut(s) 56, 818
BsnI GGCC 1 cut(s) 383
Bsp119I TTCGAA 2 cut(s) 420, 438
Bsp13I TCCGGA 1 cut(s) 92
Bsp143I GATC 3 cut(s) 181, 228, 607
Bsp1720I GCTNAGC 1 cut(s) 329
BspACI CCGC 4 cut(s) 343, 365, 380, 716
BspANI GGCC 1 cut(s) 383
BspEI TCCGGA 1 cut(s) 92
BspFNI CGCG 4 cut(s) 365, 380, 666, 725
BspPI GGATC 1 cut(s) 602
BspT104I TTCGAA 2 cut(s) 420, 438
BsrBI CCGCTC 1 cut(s) 716
BsrFI RCCGGY 3 cut(s) 15, 513, 523
BssAI RCCGGY 3 cut(s) 15, 513, 523
BssECI CCNNGG 2 cut(s) 271, 384
BssMI GATC 3 cut(s) 181, 228, 607
BssNAI GTATAC 1 cut(s) 152
BssT1I CCWWGG 1 cut(s) 384
Bst1107I GTATAC 1 cut(s) 152
Bst4CI ACNGT 4 cut(s) 50, 275, 638, 682
BstBI TTCGAA 2 cut(s) 420, 438
BstDEI CTNAG 5 cut(s) 86, 156, 221, 329, 840
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 1 cut(s) 799
BstFNI CGCG 4 cut(s) 365, 380, 666, 725
BstKTI GATC 3 cut(s) 184, 231, 610
BstMAI GTCTC 2 cut(s) 56, 818
BstMBI GATC 3 cut(s) 181, 228, 607
BstMCI CGRYCG 3 cut(s) 231, 277, 528
BstMWI GCNNNNNNNGC 2 cut(s) 386, 722
BstPAI GACNNNNGTC 1 cut(s) 704
BstUI CGCG 4 cut(s) 365, 380, 666, 725
BstV1I GCAGC 2 cut(s) 330, 454
BstZ17I GTATAC 1 cut(s) 152
Bsu36I CCTNAGG 1 cut(s) 156
BsuRI GGCC 1 cut(s) 383
BtgI CCRYGG 1 cut(s) 271
BtsCI GGATG 1 cut(s) 799
BtsIMutI CAGTG 1 cut(s) 643
Cfr10I RCCGGY 3 cut(s) 15, 513, 523
CseI GACGC 1 cut(s) 459
Csp6I GTAC 4 cut(s) 19, 43, 256, 650
CspAI ACCGGT 3 cut(s) 15, 513, 523
CviAII CATG 3 cut(s) 133, 454, 745
CviJI RGCY 9 cut(s) 90, 215, 264, 321, 333, 383, 587, 656, 839
CviKI_1 RGCY 9 cut(s) 90, 215, 264, 321, 333, 383, 587, 656, 839
CviQI GTAC 4 cut(s) 19, 43, 256, 650
DdeI CTNAG 5 cut(s) 86, 156, 221, 329, 840
DpnI GATC 3 cut(s) 183, 230, 609
DpnII GATC 3 cut(s) 181, 228, 607
DrdI GACNNNNNNGTC 1 cut(s) 167
DseDI GACNNNNNNGTC 1 cut(s) 167
EaeI YGGCCR 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 384
Eco32I GATATC 1 cut(s) 496
Eco81I CCTNAGG 1 cut(s) 156
EcoRV GATATC 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 384
FaeI CATG 3 cut(s) 136, 457, 748
FatI CATG 3 cut(s) 132, 453, 744
FauNDI CATATG 1 cut(s) 431
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 3 cut(s) 319, 381, 468
FokI GGATG 1 cut(s) 806
Fsp4HI GCNGC 3 cut(s) 319, 381, 468
GluI GCNGC 3 cut(s) 319, 381, 468
HaeIII GGCC 1 cut(s) 383
HapII CCGG 6 cut(s) 16, 93, 514, 524, 761, 807
HgaI GACGC 1 cut(s) 459
Hin1II CATG 3 cut(s) 136, 457, 748
HinfI GANTC 4 cut(s) 96, 426, 569, 773
HpaII CCGG 6 cut(s) 16, 93, 514, 524, 761, 807
Hpy166II GTNNAC 6 cut(s) 152, 271, 281, 376, 533, 560
Hpy188I TCNGA 8 cut(s) 181, 233, 484, 520, 568, 644, 778, 793
Hpy188III TCNNGA 4 cut(s) 10, 93, 580, 590
Hpy8I GTNNAC 6 cut(s) 152, 271, 281, 376, 533, 560
Hpy99I CGWCG 3 cut(s) 37, 173, 793
HpyAV CCTTC 1 cut(s) 416
HpyCH4III ACNGT 4 cut(s) 50, 275, 638, 682
HpyCH4IV ACGT 2 cut(s) 32, 648
HpyCH4V TGCA 2 cut(s) 29, 457
HpyF10VI GCNNNNNNNGC 2 cut(s) 386, 722
HpyF3I CTNAG 5 cut(s) 86, 156, 221, 329, 840
HpySE526I ACGT 2 cut(s) 32, 648
Hsp92II CATG 3 cut(s) 136, 457, 748
Kpn2I TCCGGA 1 cut(s) 92
Kzo9I GATC 3 cut(s) 181, 228, 607
LmnI GCTCC 2 cut(s) 95, 713
Lsp1109I GCAGC 2 cut(s) 330, 454
MaeII ACGT 2 cut(s) 32, 648
MalI GATC 3 cut(s) 183, 230, 609
MbiI CCGCTC 1 cut(s) 716
MboI GATC 3 cut(s) 181, 228, 607
MboII GAAGA 3 cut(s) 176, 305, 406
MluCI AATT 3 cut(s) 173, 239, 478
MluI ACGCGT 2 cut(s) 664, 723
MmeI TCCRAC 5 cut(s) 481, 708, 771, 778, 801
MnlI CCTC 8 cut(s) 93, 332, 344, 348, 403, 461, 625, 745
MroI TCCGGA 1 cut(s) 92
MroXI GAANNNNTTC 1 cut(s) 735
MseI TTAA 1 cut(s) 510
MspI CCGG 6 cut(s) 16, 93, 514, 524, 761, 807
MvnI CGCG 4 cut(s) 365, 380, 666, 725
MwoI GCNNNNNNNGC 2 cut(s) 386, 722
NdeI CATATG 1 cut(s) 431
NdeII GATC 3 cut(s) 181, 228, 607
NlaIII CATG 3 cut(s) 136, 457, 748
NspV TTCGAA 2 cut(s) 420, 438
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PdmI GAANNNNTTC 1 cut(s) 735
PfeI GAWTC 4 cut(s) 96, 426, 569, 773
PflFI GACNNNGTC 1 cut(s) 638
PinAI ACCGGT 3 cut(s) 15, 513, 523
PkrI GCNGC 3 cut(s) 320, 382, 469
Ple19I CGATCG 1 cut(s) 231
PshAI GACNNNNGTC 1 cut(s) 704
PsyI GACNNNGTC 1 cut(s) 638
PvuI CGATCG 1 cut(s) 231
RsaI GTAC 4 cut(s) 20, 44, 257, 651
RsaNI GTAC 4 cut(s) 19, 43, 256, 650
SaqAI TTAA 1 cut(s) 510
SatI GCNGC 3 cut(s) 319, 381, 468
Sau3AI GATC 3 cut(s) 181, 228, 607
SfuI TTCGAA 2 cut(s) 420, 438
Sse9I AATT 3 cut(s) 173, 239, 478
SsiI CCGC 4 cut(s) 343, 365, 380, 716
SspI AATATT 1 cut(s) 604
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 4 cut(s) 50, 275, 638, 682
TaiI ACGT 2 cut(s) 35, 651
TaqI TCGA 7 cut(s) 9, 60, 171, 420, 438, 579, 698
TaqII GACCGA 1 cut(s) 152
TasI AATT 3 cut(s) 173, 239, 478
TauI GCSGC 1 cut(s) 383
TfiI GAWTC 4 cut(s) 96, 426, 569, 773
Tru1I TTAA 1 cut(s) 510
Tru9I TTAA 1 cut(s) 510
TscAI CASTG 1 cut(s) 643
TseI GCWGC 2 cut(s) 318, 467
TspDTI ATGAA 2 cut(s) 252, 761
TspGWI ACGGA 1 cut(s) 36
TspRI CASTG 1 cut(s) 643
Tth111I GACNNNGTC 1 cut(s) 638
XapI RAATTY 1 cut(s) 239
XmiI GTMKAC 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.