pycom12g13850

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011735
Physical Location & Seq
Reverse (-)
16321511 .. 16324474
2964 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g13850.4

Sequence Viewer

Length: 1200 bp
ATGGAGATTGTGGCAAAACTGGAAATGTCTGGACTACAAGGAAACTCGTGGGAATTTCTCAACGGTTTTGCCTATTTAAACCCACCTCCTCTCCTCTCATTCCTCCTCCCACTCCTTCGTTCTTCAATACCTTTAGCGTTGTGCTCTCCCTCTCCCTCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTCCCAGAGTTCTCTTCTTGCAAGAAAGAAACTCTCTCTTTCTCTCTAGTTCATCAATTTGGTATTGGCTTTCTAATCTGGTTTCCTGAAATGGAATTCGACGATCGATTCATGCAGGCTCAGAGGACAAAGTTCGACTGCCTTTTGTTCGATCTTGATGACACACTGTATCCCTATAGTAGTGGCATTGCTACAGCATGCAGAATCAATATAGAAGATTATATGGTTGAAAAGCTTGGCATTGACAGGAGCATAATCCCTGAGTTGGGTAACCTTCTGTACAAGAATTATGGAACAACAATGGCTGGTCTCAGGGCAATTGGCTATGACTTTGACTACGATGAATATCACAGTTTTGTCCATGGAAGATTACCTTATGAGAACATAAAACCGGACCCAATTTTGAGGAGCCTCTTGCTGAACCTGCCTTACAGGAAAATTGTAAGCACCTACTGTGTAACCGACAAGATCGATGCTGCTAAAGCACTAAGCAGGCTTGGATTGGAAGGCATTTTTGAAGGGATTATCTGCTTTGAGACCCTCAATCCGATTCACAAAAACACGGTCTCTGACGACGAAGACGACATAGAATTCGTGGGATTAAGCAGCACTACCACCACTACTAGCAGCTCCCAGATTTTTGACATCATTGGCCATTTTGCTACACCAAACCCCACTTCCAAACTACCCAAAACTCCAATTGTCTGCAAACCATCCGAAGATGCCATCGAACGGGCTCTCAAGATAGCCAACATTAACCCTCAGAGAACATTATTCTTTGAAGATAGTGTCCGCAACATACAAGCTGGCAAAAGAGTGGGACTTCAAACTGTATTGGTTGGCACATCTCAGAGAGTAAAAGGTGCAGATTATGCTTTGGAAAGCATTCACAACCTCAGGGAAGCAATACCAGAGCTTTGGGAGGCTGATAGGAAATCTAAAGTTGGTTACTCTGGGAAGGTTGCAGTGGAGACATCAGTCACAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 map00760
Pfam Domains
Protein Families
HAD_2 Hydrolase_like

Protein Analysis

400

Amino Acids

44.44

Weight (kDa)

5.16

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011735)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02230 AT5G02230 AT5G02230
fragaria_vesca FvH4_6g13490 FvH4_6g13490
malus_domestica MD04G1130800.v1.1 MD12G1143900.v1.1
prunus_persica Prupe.6G257300_v2.0.a1 Prupe.6G257300_v2.0.a1 Prupe.6G257300_v2.0.a1
pyrus_communis pycom12g13850
rosa_chinensis RchiOBHm_Chr3g0466361
rosa_laevigata RLG00000024581
rosa_multiflora Rmu_sc0003480.1_g000006
rosa_roxburghii Rroxscaffold_6G00414540
rosa_rugosa Rorug03G0081300
rosa_samantha Rh3AG127700 Rh3DG149700
rosa_wichuraiana Rw3G012060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 642
AciI CCGC 1 cut(s) 1003
AcoI YGGCCR 1 cut(s) 862
AcsI RAATTY 3 cut(s) 53, 305, 800
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 3 cut(s) 475, 476, 942
AgsI TTSAA 5 cut(s) 126, 440, 728, 992, 1037
AjuI GAANNNNNNNTTGG 2 cut(s) 871, 903
AluBI AGCT 5 cut(s) 445, 840, 1016, 1126, 1196
AluI AGCT 5 cut(s) 445, 840, 1016, 1126, 1196
Alw21I GWGCWC 1 cut(s) 146
Alw26I GTCTC 4 cut(s) 524, 740, 781, 1175
AoxI GGCC 1 cut(s) 862
ApeKI GCWGC 3 cut(s) 686, 816, 837
ApoI RAATTY 3 cut(s) 53, 305, 800
ArsI GACNNNNNNTTYG 4 cut(s) 759, 785, 791, 817
Asp700I GAANNNNTTC 1 cut(s) 1095
AspS9I GGNCC 1 cut(s) 604
AvaII GGWCC 1 cut(s) 604
AxyI CCTNAGG 1 cut(s) 1106
BalI TGGCCA 1 cut(s) 864
BanII GRGCYC 1 cut(s) 949
BauI CACGAG 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 795
Bbv12I GWGCWC 1 cut(s) 146
BbvI GCAGC 3 cut(s) 673, 828, 849
BccI CCATC 2 cut(s) 931, 944
BciVI GTATCC 1 cut(s) 390
BcoDI GTCTC 4 cut(s) 524, 740, 781, 1175
BfaI CTAG 2 cut(s) 257, 834
BfmI CTRYAG 2 cut(s) 385, 402
BfuAI ACCTGC 1 cut(s) 642
BfuI GTATCC 1 cut(s) 390
BisI GCNGC 3 cut(s) 687, 817, 838
BlsI GCNGC 3 cut(s) 688, 818, 839
Bme18I GGWCC 1 cut(s) 604
BmgT120I GGNCC 1 cut(s) 604
BmiI GGNNCC 2 cut(s) 606, 620
BmsI GCATC 2 cut(s) 673, 922
BoxI GACNNNNGTC 1 cut(s) 1187
BpiI GAAGAC 1 cut(s) 795
BpuEI CTTGAG 1 cut(s) 935
Bsa29I ATCGAT 2 cut(s) 316, 681
BsaBI GATNNNNATC 1 cut(s) 555
BsaI GGTCTC 3 cut(s) 524, 740, 781
BsaJI CCNNGG 1 cut(s) 571
BsaWI WCCGGW 1 cut(s) 601
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 3 cut(s) 475, 476, 942
Bse1I ACTGG 1 cut(s) 24
Bse21I CCTNAGG 1 cut(s) 1106
Bse3DI GCAATG 1 cut(s) 396
Bse8I GATNNNNATC 1 cut(s) 555
BseCI ATCGAT 2 cut(s) 316, 681
BseDI CCNNGG 1 cut(s) 571
BseGI GGATG 1 cut(s) 923
BseJI GATNNNNATC 1 cut(s) 555
BseLI CCNNNNNNNGG 3 cut(s) 475, 476, 942
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 6 cut(s) 344, 462, 535, 986, 1073, 1120
BseNI ACTGG 1 cut(s) 24
BseRI GAGGAG 4 cut(s) 78, 83, 95, 631
BseXI GCAGC 3 cut(s) 673, 828, 849
BsgI GTGCAG 1 cut(s) 1095
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 1 cut(s) 864
BshVI ATCGAT 2 cut(s) 316, 681
BsiEI CGRYCG 1 cut(s) 316
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 1 cut(s) 602
BslFI GGGAC 1 cut(s) 1044
BslI CCNNNNNNNGG 3 cut(s) 475, 476, 942
BsmAI GTCTC 4 cut(s) 524, 740, 781, 1175
BsmFI GGGAC 1 cut(s) 1044
BsmI GAATGC 1 cut(s) 1095
BsnI GGCC 1 cut(s) 864
Bso31I GGTCTC 3 cut(s) 524, 740, 781
Bsp1286I GDGCHC 2 cut(s) 146, 949
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 3 cut(s) 313, 361, 678
Bsp19I CCATGG 1 cut(s) 571
BspACI CCGC 1 cut(s) 1003
BspANI GGCC 1 cut(s) 864
BspCNI CTCAG 6 cut(s) 343, 463, 534, 985, 1072, 1119
BspDI ATCGAT 2 cut(s) 316, 681
BspLI GGNNCC 2 cut(s) 606, 620
BspMI ACCTGC 1 cut(s) 642
BspTNI GGTCTC 3 cut(s) 524, 740, 781
BsrDI GCAATG 1 cut(s) 396
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 1 cut(s) 24
BssECI CCNNGG 1 cut(s) 571
BssMI GATC 3 cut(s) 313, 361, 678
BssSI CACGAG 1 cut(s) 46
BssT1I CCWWGG 1 cut(s) 571
Bst2BI CACGAG 1 cut(s) 46
Bst4CI ACNGT 6 cut(s) 65, 378, 563, 665, 775, 1042
Bst6I CTCTTC 2 cut(s) 215, 229
BstAPI GCANNNNNTGC 1 cut(s) 1082
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 4 cut(s) 327, 409, 704, 1018
BstDEI CTNAG 8 cut(s) 330, 471, 521, 698, 972, 1059, 1106, 1197
BstDSI CCRYGG 1 cut(s) 571
BstEII GGTNACC 1 cut(s) 479
BstF5I GGATG 1 cut(s) 923
BstKTI GATC 3 cut(s) 316, 364, 681
BstMAI GTCTC 4 cut(s) 524, 740, 781, 1175
BstMBI GATC 3 cut(s) 313, 361, 678
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 3 cut(s) 634, 692, 1082
BstNSI RCATGY 1 cut(s) 411
BstPAI GACNNNNGTC 1 cut(s) 1187
BstPI GGTNACC 1 cut(s) 479
BstSFI CTRYAG 2 cut(s) 385, 402
BstV1I GCAGC 3 cut(s) 673, 828, 849
BstV2I GAAGAC 1 cut(s) 795
BstXI CCANNNNNNTGG 1 cut(s) 1128
Bsu15I ATCGAT 2 cut(s) 316, 681
Bsu36I CCTNAGG 1 cut(s) 1106
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 1 cut(s) 864
BsuTUI ATCGAT 2 cut(s) 316, 681
BtgI CCRYGG 1 cut(s) 571
BtsCI GGATG 1 cut(s) 923
BtsI GCAGTG 1 cut(s) 1182
BtsIMutI CAGTG 2 cut(s) 374, 1182
BveI ACCTGC 1 cut(s) 642
Cac8I GCNNGC 4 cut(s) 327, 409, 704, 1018
Cfr13I GGNCC 1 cut(s) 604
ClaI ATCGAT 2 cut(s) 316, 681
Csp6I GTAC 1 cut(s) 490
CviAII CATG 3 cut(s) 322, 408, 572
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 8 cut(s) 330, 471, 521, 698, 972, 1059, 1106, 1197
DpnI GATC 3 cut(s) 315, 363, 680
DpnII GATC 3 cut(s) 313, 361, 678
DraI TTTAAA 1 cut(s) 78
EaeI YGGCCR 1 cut(s) 862
Eam1104I CTCTTC 2 cut(s) 215, 229
EarI CTCTTC 2 cut(s) 215, 229
Eco130I CCWWGG 1 cut(s) 571
Eco24I GRGCYC 1 cut(s) 949
Eco31I GGTCTC 3 cut(s) 524, 740, 781
Eco47I GGWCC 1 cut(s) 604
Eco81I CCTNAGG 1 cut(s) 1106
Eco91I GGTNACC 1 cut(s) 479
EcoO65I GGTNACC 1 cut(s) 479
EcoRI GAATTC 2 cut(s) 305, 800
EcoT14I CCWWGG 1 cut(s) 571
EcoT38I GRGCYC 1 cut(s) 949
ErhI CCWWGG 1 cut(s) 571
FaeI CATG 3 cut(s) 325, 411, 575
FalI AAGNNNNNCTT 2 cut(s) 568, 600
FaqI GGGAC 1 cut(s) 1044
FatI CATG 3 cut(s) 321, 407, 571
Fnu4HI GCNGC 3 cut(s) 687, 817, 838
FokI GGATG 1 cut(s) 910
FriOI GRGCYC 1 cut(s) 949
Fsp4HI GCNGC 3 cut(s) 687, 817, 838
FspBI CTAG 2 cut(s) 257, 834
GluI GCNGC 3 cut(s) 687, 817, 838
HaeIII GGCC 1 cut(s) 864
HapII CCGG 1 cut(s) 602
Hin1II CATG 3 cut(s) 325, 411, 575
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 3 cut(s) 318, 414, 760
HpaII CCGG 1 cut(s) 602
Hpy188I TCNGA 6 cut(s) 333, 759, 781, 928, 975, 1062
Hpy188III TCNNGA 4 cut(s) 30, 296, 365, 952
Hpy99I CGWCG 2 cut(s) 314, 788
HpyAV CCTTC 5 cut(s) 125, 494, 710, 722, 1162
HpyCH4III ACNGT 6 cut(s) 65, 378, 563, 665, 775, 1042
HpyCH4V TGCA 6 cut(s) 231, 325, 411, 918, 1076, 1175
HpyF10VI GCNNNNNNNGC 3 cut(s) 634, 692, 1082
HpyF3I CTNAG 8 cut(s) 330, 471, 521, 698, 972, 1059, 1106, 1197
Hsp92II CATG 3 cut(s) 325, 411, 575
Kzo9I GATC 3 cut(s) 313, 361, 678
LmnI GCTCC 3 cut(s) 459, 618, 845
Lsp1109I GCAGC 3 cut(s) 673, 828, 849
LweI GCATC 2 cut(s) 673, 922
MaeI CTAG 2 cut(s) 257, 834
MaeIII GTNAC 4 cut(s) 479, 667, 1157, 1189
MalI GATC 3 cut(s) 315, 363, 680
MboI GATC 3 cut(s) 313, 361, 678
MboII GAAGA 8 cut(s) 114, 202, 216, 437, 588, 800, 941, 1004
MfeI CAATTG 2 cut(s) 528, 909
MhlI GDGCHC 2 cut(s) 146, 949
MlsI TGGCCA 1 cut(s) 864
MluCI AATT 9 cut(s) 53, 266, 305, 496, 528, 609, 648, 800, 909
MluNI TGGCCA 1 cut(s) 864
Mox20I TGGCCA 1 cut(s) 864
MroXI GAANNNNTTC 1 cut(s) 1095
MscI TGGCCA 1 cut(s) 864
MseI TTAA 3 cut(s) 77, 812, 966
Msp20I TGGCCA 1 cut(s) 864
MspI CCGG 1 cut(s) 602
MunI CAATTG 2 cut(s) 528, 909
Mva1269I GAATGC 1 cut(s) 1095
MwoI GCNNNNNNNGC 3 cut(s) 634, 692, 1082
NcoI CCATGG 1 cut(s) 571
NdeII GATC 3 cut(s) 313, 361, 678
NlaIII CATG 3 cut(s) 325, 411, 575
NlaIV GGNNCC 2 cut(s) 606, 620
NmuCI GTSAC 1 cut(s) 1189
NspI RCATGY 1 cut(s) 411
PaeI GCATGC 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 789
PctI GAATGC 1 cut(s) 1095
PdmI GAANNNNTTC 1 cut(s) 1095
PfeI GAWTC 3 cut(s) 318, 414, 760
PkrI GCNGC 3 cut(s) 688, 818, 839
Ple19I CGATCG 1 cut(s) 316
PshAI GACNNNNGTC 1 cut(s) 1187
PspEI GGTNACC 1 cut(s) 479
PspN4I GGNNCC 2 cut(s) 606, 620
PspPI GGNCC 1 cut(s) 604
PsrI GAACNNNNNNTAC 2 cut(s) 623, 655
PvuI CGATCG 1 cut(s) 316
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
SaqAI TTAA 3 cut(s) 77, 812, 966
SatI GCNGC 3 cut(s) 687, 817, 838
Sau3AI GATC 3 cut(s) 313, 361, 678
Sau96I GGNCC 1 cut(s) 604
SduI GDGCHC 2 cut(s) 146, 949
SfaNI GCATC 2 cut(s) 673, 922
SfcI CTRYAG 2 cut(s) 385, 402
SinI GGWCC 1 cut(s) 604
SmlI CTYRAG 1 cut(s) 950
SmoI CTYRAG 1 cut(s) 950
SphI GCATGC 1 cut(s) 411
Sse9I AATT 9 cut(s) 53, 266, 305, 496, 528, 609, 648, 800, 909
SsiI CCGC 1 cut(s) 1003
SspMI CTAG 2 cut(s) 257, 834
StyI CCWWGG 1 cut(s) 571
TaaI ACNGT 6 cut(s) 65, 378, 563, 665, 775, 1042
TaqI TCGA 6 cut(s) 309, 316, 345, 360, 681, 939
TasI AATT 9 cut(s) 53, 266, 305, 496, 528, 609, 648, 800, 909
TatI WGTACW 1 cut(s) 489
TfiI GAWTC 3 cut(s) 318, 414, 760
Tru1I TTAA 3 cut(s) 77, 812, 966
Tru9I TTAA 3 cut(s) 77, 812, 966
TscAI CASTG 2 cut(s) 381, 1182
TseFI GTSAC 1 cut(s) 1189
TseI GCWGC 3 cut(s) 686, 816, 837
Tsp45I GTSAC 1 cut(s) 1189
TspDTI ATGAA 3 cut(s) 251, 310, 567
TspRI CASTG 2 cut(s) 381, 1182
VpaK11BI GGWCC 1 cut(s) 604
XapI RAATTY 3 cut(s) 53, 305, 800
XceI RCATGY 1 cut(s) 411
XmnI GAANNNNTTC 1 cut(s) 1095
XspI CTAG 2 cut(s) 257, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.