Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0011735

Physical Location & Seq

Genomic Coordinates Reverse (-)

21746599 .. 21749242

Gene Structure 2644 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1130800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 873 bp

ATGCAGGCTCAGAGGACAAAGTACGATTGCCTTTTGTTCGATCTTGATGATACACTGTATCCCTATAGTAGTGGGATTGCCACAGCATGCAGAATCAACATAGAAGATTATATGGTTGAAAAGCTTGGCATTGACAGGAGCATAATCCCTGAGTTGGGTAACCTTCTGTACAAGAATTATGGAACAACAATGGCTGGTCTCAGGGCAATTGGCTATGACTTTGACTACGATAAATATCACAGTTTTGTCCATGGAAGATTACCTTATGACAACATAAAACCCGACCCGGTTTTGAGGAGCCTCTTGCTGAACCTGCCTTACAGGAAGATTATATTCACAAATGCAGACAAGATCCATGCTGCTAAAGCACTAAGCAGGCTTGGATTGGAAGACATTTTTGAAGGGATTATCTGCTTTGAGACCCTCAATCCGATTCACAAAAACACTGTCTCTGACGACGAAGATGACATCGAATTCGTGGGATTAAGCAGCACTACCTCCATTACTAGCAGCTCTCAAATTTTTGACATCATTGGCCATTTTGCTACACCAAACCCCACTTCCAAACTACCCAAAACTCCAATCGTCTGCAAACCATCTGAAGACTCCATCGAACGGGCTCTCAAGATAGCCAACATCAACCCTCAAAGAACATTGTTCTTTGAAGATAGTGTCCGCAACATACAAGCTGGCAAAAGAGTGGGACTTCAAACTGTACTGGTTGGCACATCTCAGAGAGTAAAAGGTGCAGATTTTGCTTTGGAAAGCATTCACAACCTCAGAGAAGCAATACCAGAGCTTTGGGAGGCTGATAGGAAATCTGAAGTTGGTTACTCTGGGAAGGTTGCTGTGGAGACATCAGTCACGGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00760 map00760

Pfam Domains

Protein Families

HAD_2 Hydrolase_like

Protein Analysis

291

Amino Acids

32.41

Weight (kDa)

5.45

Isoelectric Point (pI)

43.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	191 - 235	8.6e-06	haloacid dehalogenase-like hydrolase
HAD_2	PF13419	198 - 241	6.2e-07	Haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011735)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02230 AT5G02230 AT5G02230
fragaria_vesca	FvH4_6g13490 FvH4_6g13490
malus_domestica	MD04G1130800.v1.1 MD12G1143900.v1.1
prunus_persica	Prupe.6G257300_v2.0.a1 Prupe.6G257300_v2.0.a1 Prupe.6G257300_v2.0.a1
pyrus_communis	pycom12g13850
rosa_chinensis	RchiOBHm_Chr3g0466361
rosa_laevigata	RLG00000024581
rosa_multiflora	Rmu_sc0003480.1_g000006
rosa_roxburghii	Rroxscaffold_6G00414540
rosa_rugosa	Rorug03G0081300
rosa_samantha	Rh3AG127700 Rh3DG149700
rosa_wichuraiana	Rw3G012060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	321
AciI	CCGC	1 cut(s)	676
AclWI	GGATC	1 cut(s)	346
AcoI	YGGCCR	1 cut(s)	535
AcsI	RAATTY	2 cut(s)	473, 519
AcuI	CTGAAG	2 cut(s)	621, 843
AfaI	GTAC	3 cut(s)	23, 170, 717
AfiI	CCNNNNNNNGG	3 cut(s)	154, 155, 615
AgsI	TTSAA	4 cut(s)	119, 401, 665, 710
AjuI	GAANNNNNNNTTGG	2 cut(s)	544, 576
AluBI	AGCT	4 cut(s)	124, 513, 689, 799
AluI	AGCT	4 cut(s)	124, 513, 689, 799
Alw26I	GTCTC	4 cut(s)	203, 413, 454, 848
AlwI	GGATC	1 cut(s)	346
AoxI	GGCC	1 cut(s)	535
ApeKI	GCWGC	3 cut(s)	359, 489, 510
ApoI	RAATTY	2 cut(s)	473, 519
ArsI	GACNNNNNNTTYG	4 cut(s)	432, 458, 464, 490
Asp700I	GAANNNNTTC	1 cut(s)	768
AsuC2I	CCSGG	1 cut(s)	287
BalI	TGGCCA	1 cut(s)	537
BanII	GRGCYC	1 cut(s)	622
BbsI	GAAGAC	2 cut(s)	396, 609
BbvI	GCAGC	3 cut(s)	346, 501, 522
BccI	CCATC	2 cut(s)	604, 617
BciVI	GTATCC	1 cut(s)	69
BcnI	CCSGG	1 cut(s)	287
BcoDI	GTCTC	4 cut(s)	203, 413, 454, 848
BfaI	CTAG	1 cut(s)	507
BfmI	CTRYAG	1 cut(s)	64
BfuAI	ACCTGC	1 cut(s)	321
BfuI	GTATCC	1 cut(s)	69
BisI	GCNGC	3 cut(s)	360, 490, 511
BlsI	GCNGC	3 cut(s)	361, 491, 512
Bme1390I	CCNGG	1 cut(s)	287
BmiI	GGNNCC	1 cut(s)	299
BmrFI	CCNGG	1 cut(s)	287
BoxI	GACNNNNGTC	1 cut(s)	860
BpiI	GAAGAC	2 cut(s)	396, 609
BpuEI	CTTGAG	1 cut(s)	608
BpuMI	CCSGG	1 cut(s)	287
BsaBI	GATNNNNATC	1 cut(s)	234
BsaI	GGTCTC	2 cut(s)	203, 413
BsaJI	CCNNGG	1 cut(s)	250
Bsc4I	CCNNNNNNNGG	3 cut(s)	154, 155, 615
Bse1I	ACTGG	1 cut(s)	723
Bse8I	GATNNNNATC	1 cut(s)	234
BseDI	CCNNGG	1 cut(s)	250
BseJI	GATNNNNATC	1 cut(s)	234
BseLI	CCNNNNNNNGG	3 cut(s)	154, 155, 615
BseMII	CTCAG	5 cut(s)	23, 141, 214, 746, 793
BseNI	ACTGG	1 cut(s)	723
BseRI	GAGGAG	1 cut(s)	310
BseXI	GCAGC	3 cut(s)	346, 501, 522
BsgI	GTGCAG	1 cut(s)	768
BshFI	GGCC	1 cut(s)	537
BsiSI	CCGG	1 cut(s)	287
BslFI	GGGAC	1 cut(s)	717
BslI	CCNNNNNNNGG	3 cut(s)	154, 155, 615
BsmAI	GTCTC	4 cut(s)	203, 413, 454, 848
BsmFI	GGGAC	1 cut(s)	717
BsmI	GAATGC	1 cut(s)	768
BsnI	GGCC	1 cut(s)	537
Bso31I	GGTCTC	2 cut(s)	203, 413
Bsp1286I	GDGCHC	1 cut(s)	622
Bsp1407I	TGTACA	1 cut(s)	168
Bsp143I	GATC	2 cut(s)	40, 351
Bsp19I	CCATGG	1 cut(s)	250
BspACI	CCGC	1 cut(s)	676
BspANI	GGCC	1 cut(s)	537
BspCNI	CTCAG	5 cut(s)	22, 142, 213, 745, 792
BspLI	GGNNCC	1 cut(s)	299
BspMI	ACCTGC	1 cut(s)	321
BspPI	GGATC	1 cut(s)	346
BspTNI	GGTCTC	2 cut(s)	203, 413
BsrGI	TGTACA	1 cut(s)	168
BsrI	ACTGG	1 cut(s)	723
BssECI	CCNNGG	1 cut(s)	250
BssMI	GATC	2 cut(s)	40, 351
BssT1I	CCWWGG	1 cut(s)	250
Bst4CI	ACNGT	4 cut(s)	57, 242, 448, 715
BstAPI	GCANNNNNTGC	1 cut(s)	755
BstAUI	TGTACA	1 cut(s)	168
BstC8I	GCNNGC	4 cut(s)	6, 88, 377, 691
BstDEI	CTNAG	7 cut(s)	9, 150, 200, 371, 732, 779, 870
BstDSI	CCRYGG	1 cut(s)	250
BstEII	GGTNACC	1 cut(s)	158
BstKTI	GATC	2 cut(s)	43, 354
BstMAI	GTCTC	4 cut(s)	203, 413, 454, 848
BstMBI	GATC	2 cut(s)	40, 351
BstMWI	GCNNNNNNNGC	3 cut(s)	313, 365, 755
BstNSI	RCATGY	1 cut(s)	90
BstPAI	GACNNNNGTC	1 cut(s)	860
BstPI	GGTNACC	1 cut(s)	158
BstSCI	CCNGG	1 cut(s)	285
BstSFI	CTRYAG	1 cut(s)	64
BstV1I	GCAGC	3 cut(s)	346, 501, 522
BstV2I	GAAGAC	2 cut(s)	396, 609
BstX2I	RGATCY	1 cut(s)	351
BstXI	CCANNNNNNTGG	1 cut(s)	801
BstYI	RGATCY	1 cut(s)	351
BsuI	GTATCC	1 cut(s)	69
BsuRI	GGCC	1 cut(s)	537
BtgI	CCRYGG	1 cut(s)	250
BtsIMutI	CAGTG	2 cut(s)	53, 444
BveI	ACCTGC	1 cut(s)	321
Cac8I	GCNNGC	4 cut(s)	6, 88, 377, 691
Csp6I	GTAC	3 cut(s)	22, 169, 716
CviAII	CATG	3 cut(s)	87, 251, 356
CviQI	GTAC	3 cut(s)	22, 169, 716
DdeI	CTNAG	7 cut(s)	9, 150, 200, 371, 732, 779, 870
DpnI	GATC	2 cut(s)	42, 353
DpnII	GATC	2 cut(s)	40, 351
EaeI	YGGCCR	1 cut(s)	535
Eco130I	CCWWGG	1 cut(s)	250
Eco24I	GRGCYC	1 cut(s)	622
Eco31I	GGTCTC	2 cut(s)	203, 413
Eco57I	CTGAAG	2 cut(s)	621, 843
Eco91I	GGTNACC	1 cut(s)	158
EcoO65I	GGTNACC	1 cut(s)	158
EcoRI	GAATTC	1 cut(s)	473
EcoT14I	CCWWGG	1 cut(s)	250
EcoT38I	GRGCYC	1 cut(s)	622
ErhI	CCWWGG	1 cut(s)	250
FaeI	CATG	3 cut(s)	90, 254, 359
FalI	AAGNNNNNCTT	2 cut(s)	247, 279
FaqI	GGGAC	1 cut(s)	717
FatI	CATG	3 cut(s)	86, 250, 355
Fnu4HI	GCNGC	3 cut(s)	360, 490, 511
FriOI	GRGCYC	1 cut(s)	622
Fsp4HI	GCNGC	3 cut(s)	360, 490, 511
FspBI	CTAG	1 cut(s)	507
GluI	GCNGC	3 cut(s)	360, 490, 511
HaeIII	GGCC	1 cut(s)	537
HapII	CCGG	1 cut(s)	287
Hin1II	CATG	3 cut(s)	90, 254, 359
HindIII	AAGCTT	1 cut(s)	122
HinfI	GANTC	3 cut(s)	93, 433, 605
HpaII	CCGG	1 cut(s)	287
Hpy188I	TCNGA	7 cut(s)	12, 432, 454, 601, 735, 782, 823
Hpy188III	TCNNGA	2 cut(s)	44, 625
Hpy99I	CGWCG	1 cut(s)	461
HpyAV	CCTTC	3 cut(s)	173, 395, 835
HpyCH4III	ACNGT	4 cut(s)	57, 242, 448, 715
HpyCH4V	TGCA	5 cut(s)	4, 90, 344, 591, 749
HpyF10VI	GCNNNNNNNGC	3 cut(s)	313, 365, 755
HpyF3I	CTNAG	7 cut(s)	9, 150, 200, 371, 732, 779, 870
Hsp92II	CATG	3 cut(s)	90, 254, 359
Kzo9I	GATC	2 cut(s)	40, 351
LmnI	GCTCC	2 cut(s)	138, 297
Lsp1109I	GCAGC	3 cut(s)	346, 501, 522
MaeI	CTAG	1 cut(s)	507
MaeIII	GTNAC	3 cut(s)	158, 830, 862
MalI	GATC	2 cut(s)	42, 353
MboI	GATC	2 cut(s)	40, 351
MboII	GAAGA	7 cut(s)	116, 267, 337, 401, 473, 614, 677
MfeI	CAATTG	1 cut(s)	207
MflI	RGATCY	1 cut(s)	351
MhlI	GDGCHC	1 cut(s)	622
MlsI	TGGCCA	1 cut(s)	537
MluCI	AATT	4 cut(s)	175, 207, 473, 519
MluNI	TGGCCA	1 cut(s)	537
MlyI	GAGTC	1 cut(s)	599
MnlI	CCTC	8 cut(s)	6, 288, 311, 434, 508, 654, 788, 799
Mox20I	TGGCCA	1 cut(s)	537
MroXI	GAANNNNTTC	1 cut(s)	768
MscI	TGGCCA	1 cut(s)	537
MseI	TTAA	1 cut(s)	485
Msp20I	TGGCCA	1 cut(s)	537
MspI	CCGG	1 cut(s)	287
MspR9I	CCNGG	1 cut(s)	287
MunI	CAATTG	1 cut(s)	207
Mva1269I	GAATGC	1 cut(s)	768
MwoI	GCNNNNNNNGC	3 cut(s)	313, 365, 755
NciI	CCSGG	1 cut(s)	287
NcoI	CCATGG	1 cut(s)	250
NdeII	GATC	2 cut(s)	40, 351
NlaIII	CATG	3 cut(s)	90, 254, 359
NlaIV	GGNNCC	1 cut(s)	299
NmuCI	GTSAC	1 cut(s)	862
NspI	RCATGY	1 cut(s)	90
PaeI	GCATGC	1 cut(s)	90
PctI	GAATGC	1 cut(s)	768
PdmI	GAANNNNTTC	1 cut(s)	768
PfeI	GAWTC	2 cut(s)	93, 433
PkrI	GCNGC	3 cut(s)	361, 491, 512
PleI	GAGTC	1 cut(s)	599
PpsI	GAGTC	1 cut(s)	599
PshAI	GACNNNNGTC	1 cut(s)	860
PspEI	GGTNACC	1 cut(s)	158
PspN4I	GGNNCC	1 cut(s)	299
PsrI	GAACNNNNNNTAC	2 cut(s)	302, 334
PsuI	RGATCY	1 cut(s)	351
RsaI	GTAC	3 cut(s)	23, 170, 717
RsaNI	GTAC	3 cut(s)	22, 169, 716
SaqAI	TTAA	1 cut(s)	485
SatI	GCNGC	3 cut(s)	360, 490, 511
Sau3AI	GATC	2 cut(s)	40, 351
SchI	GAGTC	1 cut(s)	599
ScrFI	CCNGG	1 cut(s)	287
SduI	GDGCHC	1 cut(s)	622
SfcI	CTRYAG	1 cut(s)	64
SmlI	CTYRAG	1 cut(s)	623
SmoI	CTYRAG	1 cut(s)	623
SphI	GCATGC	1 cut(s)	90
Sse9I	AATT	4 cut(s)	175, 207, 473, 519
SsiI	CCGC	1 cut(s)	676
SspMI	CTAG	1 cut(s)	507
StyD4I	CCNGG	1 cut(s)	285
StyI	CCWWGG	1 cut(s)	250
TaaI	ACNGT	4 cut(s)	57, 242, 448, 715
TaqI	TCGA	3 cut(s)	39, 471, 612
TasI	AATT	4 cut(s)	175, 207, 473, 519
TatI	WGTACW	2 cut(s)	168, 715
TfiI	GAWTC	2 cut(s)	93, 433
Tru1I	TTAA	1 cut(s)	485
Tru9I	TTAA	1 cut(s)	485
TscAI	CASTG	2 cut(s)	60, 451
TseFI	GTSAC	1 cut(s)	862
TseI	GCWGC	3 cut(s)	359, 489, 510
Tsp45I	GTSAC	1 cut(s)	862
TspRI	CASTG	2 cut(s)	60, 451
XapI	RAATTY	2 cut(s)	473, 519
XceI	RCATGY	1 cut(s)	90
XmnI	GAANNNNTTC	1 cut(s)	768
XspI	CTAG	1 cut(s)	507

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.