AT5G02730

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0019556
Physical Location & Seq
Forward (+)
615550 .. 616227
678 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G02730.1

Sequence Viewer

Length: 618 bp
ATGTCGTCGTCTTCGTTATATCATCCGTTTTGTCTCTCCATCTCCTCCGTTCTCCTCCTCCTCCTATTAATCTTCTCCGGAGAATTTCCCTCCACCGCAGGAACTTCATCACCGGACACCAAAGCCGCAGCCGCACGTGCGACTAACAGAGGAAGGAGGAATAAGCAATCCGCGGAGTTCTTACTCGCGCACAACGCAGCGCGTGTAGCTTCAGGCGCGTCGAATCTAAGATGGGATCAAGGGTTGGCCCGTTTCGCATCTAAATGGGCCAAACAACGTAAATCGGATTGTAAAATGACCCATTCAGGTGGGCCTTACGGAGAGAATATTTTCAGGTACCAAAGGAGTGAGAATTGGTCGCCGAGGAGAGTTGTTGATAAATGGATGGATGAAAGTTTGAACTACGATCGGGTGGCTAATACGTGCAAGTCTGGTGCCATGTGCGGTCATTATACGCAGATCGTTTGGCGTACAACCACAGCCGTTGGATGCGCTCGTTCTAAATGCGATAACAACCGTGGATTCCTAGTGATTTGCGAGTATTCACCTTCGGGGAATTACGAAGGCGAAAGTCCTTTTGATATACCTAAAATAACATTGAAATTAGACAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

205

Amino Acids

22.79

Weight (kDa)

9.51

Isoelectric Point (pI)

51.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 63 - 181 1.3e-23 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019556)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09590 AT5G02730
malus_domestica MD02G1191600.v1.1 MD15G1302700.v1.1
prunus_persica Prupe.6G214000_v2.0.a1
pyrus_communis pycom02g15640 pycom15g26600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 336
AccB1I GGYRCC 2 cut(s) 336, 434
AccII CGCG 4 cut(s) 173, 188, 202, 218
AccIII TCCGGA 1 cut(s) 77
AciI CCGC 6 cut(s) 96, 126, 132, 171, 173, 444
AclWI GGATC 1 cut(s) 243
AcsI RAATTY 1 cut(s) 83
AcuI CTGAAG 1 cut(s) 195
AcvI CACGTG 1 cut(s) 137
AfaI GTAC 2 cut(s) 338, 472
AgsI TTSAA 2 cut(s) 400, 601
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
Alw26I GTCTC 1 cut(s) 38
AlwI GGATC 1 cut(s) 243
Aor13HI TCCGGA 1 cut(s) 77
AoxI GGCC 3 cut(s) 246, 267, 311
ApeKI GCWGC 2 cut(s) 128, 197
ApoI RAATTY 1 cut(s) 83
AseI ATTAAT 1 cut(s) 68
Asp700I GAANNNNTTC 1 cut(s) 329
Asp718I GGTACC 1 cut(s) 336
AspLEI GCGC 4 cut(s) 190, 202, 218, 494
AspS9I GGNCC 3 cut(s) 247, 267, 311
AsuHPI GGTGA 2 cut(s) 102, 537
BanI GGYRCC 2 cut(s) 336, 434
BbrPI CACGTG 1 cut(s) 137
BbsI GAAGAC 1 cut(s) 3
BbvI GCAGC 2 cut(s) 140, 209
BccI CCATC 3 cut(s) 47, 225, 379
BceAI ACGGC 1 cut(s) 467
BcoDI GTCTC 1 cut(s) 38
BfaI CTAG 1 cut(s) 527
BisI GCNGC 4 cut(s) 126, 129, 132, 198
BlsI GCNGC 4 cut(s) 127, 130, 133, 199
BmgT120I GGNCC 3 cut(s) 247, 267, 311
BmiI GGNNCC 2 cut(s) 338, 436
BmsI GCATC 2 cut(s) 266, 479
BpiI GAAGAC 1 cut(s) 3
BsaAI YACGTR 2 cut(s) 137, 423
BsaJI CCNNGG 3 cut(s) 171, 362, 517
BsaWI WCCGGW 2 cut(s) 77, 112
BseAI TCCGGA 1 cut(s) 77
BseDI CCNNGG 3 cut(s) 171, 362, 517
BseGI GGATG 4 cut(s) 22, 390, 394, 494
BseRI GAGGAG 5 cut(s) 34, 44, 47, 50, 379
BseXI GCAGC 2 cut(s) 140, 209
Bsh1236I CGCG 4 cut(s) 173, 188, 202, 218
Bsh1285I CGRYCG 1 cut(s) 409
BshFI GGCC 3 cut(s) 248, 269, 313
BshNI GGYRCC 2 cut(s) 336, 434
BsiEI CGRYCG 1 cut(s) 409
BsiSI CCGG 2 cut(s) 78, 113
BsmAI GTCTC 1 cut(s) 38
BsnI GGCC 3 cut(s) 248, 269, 313
Bsp13I TCCGGA 1 cut(s) 77
Bsp143I GATC 3 cut(s) 235, 406, 459
BspACI CCGC 6 cut(s) 96, 126, 132, 171, 173, 444
BspANI GGCC 3 cut(s) 248, 269, 313
BspEI TCCGGA 1 cut(s) 77
BspFNI CGCG 4 cut(s) 173, 188, 202, 218
BspLI GGNNCC 2 cut(s) 338, 436
BspPI GGATC 1 cut(s) 243
BspT107I GGYRCC 2 cut(s) 336, 434
BssECI CCNNGG 3 cut(s) 171, 362, 517
BssMI GATC 3 cut(s) 235, 406, 459
Bst4CI ACNGT 1 cut(s) 518
BstBAI YACGTR 2 cut(s) 137, 423
BstDEI CTNAG 1 cut(s) 227
BstDSI CCRYGG 2 cut(s) 171, 517
BstF5I GGATG 4 cut(s) 22, 390, 394, 494
BstFNI CGCG 4 cut(s) 173, 188, 202, 218
BstHHI GCGC 4 cut(s) 190, 202, 218, 494
BstKTI GATC 3 cut(s) 238, 409, 462
BstMAI GTCTC 1 cut(s) 38
BstMBI GATC 3 cut(s) 235, 406, 459
BstMCI CGRYCG 1 cut(s) 409
BstMWI GCNNNNNNNGC 6 cut(s) 131, 137, 194, 206, 215, 254
BstUI CGCG 4 cut(s) 173, 188, 202, 218
BstV1I GCAGC 2 cut(s) 140, 209
BstV2I GAAGAC 1 cut(s) 3
BstXI CCANNNNNNTGG 1 cut(s) 308
BsuRI GGCC 3 cut(s) 248, 269, 313
BtgI CCRYGG 2 cut(s) 171, 517
BtsCI GGATG 4 cut(s) 22, 390, 394, 494
CfoI GCGC 4 cut(s) 190, 202, 218, 494
Cfr13I GGNCC 3 cut(s) 247, 267, 311
Cfr42I CCGCGG 1 cut(s) 174
CseI GACGC 1 cut(s) 207
Csp6I GTAC 2 cut(s) 337, 471
CspCI CAANNNNNGTGG 2 cut(s) 466, 501
CviAII CATG 1 cut(s) 439
CviJI RGCY 8 cut(s) 125, 131, 209, 248, 269, 313, 416, 482
CviKI_1 RGCY 8 cut(s) 125, 131, 209, 248, 269, 313, 416, 482
CviQI GTAC 2 cut(s) 337, 471
DdeI CTNAG 1 cut(s) 227
DpnI GATC 3 cut(s) 237, 408, 461
DpnII GATC 3 cut(s) 235, 406, 459
Eco57I CTGAAG 1 cut(s) 195
Eco72I CACGTG 1 cut(s) 137
FaeI CATG 1 cut(s) 442
FaiI YATR 4 cut(s) 19, 440, 453, 584
FatI CATG 1 cut(s) 438
Fnu4HI GCNGC 4 cut(s) 126, 129, 132, 198
FokI GGATG 4 cut(s) 9, 397, 401, 501
Fsp4HI GCNGC 4 cut(s) 126, 129, 132, 198
FspBI CTAG 1 cut(s) 527
GlaI GCGC 4 cut(s) 189, 201, 217, 493
GluI GCNGC 4 cut(s) 126, 129, 132, 198
HaeIII GGCC 3 cut(s) 248, 269, 313
HapII CCGG 2 cut(s) 78, 113
HgaI GACGC 1 cut(s) 207
HhaI GCGC 4 cut(s) 190, 202, 218, 494
Hin1II CATG 1 cut(s) 442
Hin6I GCGC 4 cut(s) 188, 200, 216, 492
HinP1I GCGC 4 cut(s) 188, 200, 216, 492
HinfI GANTC 2 cut(s) 223, 522
HpaII CCGG 2 cut(s) 78, 113
HphI GGTGA 2 cut(s) 102, 537
Hpy188I TCNGA 1 cut(s) 286
Hpy188III TCNNGA 1 cut(s) 78
Hpy99I CGWCG 2 cut(s) 10, 223
HpyAV CCTTC 3 cut(s) 147, 557, 558
HpyCH4III ACNGT 1 cut(s) 518
HpyCH4IV ACGT 3 cut(s) 136, 277, 422
HpyCH4V TGCA 1 cut(s) 426
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 137, 194, 206, 215, 254
HpyF3I CTNAG 1 cut(s) 227
HpySE526I ACGT 3 cut(s) 136, 277, 422
Hsp92II CATG 1 cut(s) 442
HspAI GCGC 4 cut(s) 188, 200, 216, 492
Kpn2I TCCGGA 1 cut(s) 77
KpnI GGTACC 1 cut(s) 340
KspI CCGCGG 1 cut(s) 174
Kzo9I GATC 3 cut(s) 235, 406, 459
LpnPI CCDG 7 cut(s) 84, 91, 126, 198, 291, 319, 417
Lsp1109I GCAGC 2 cut(s) 140, 209
LweI GCATC 2 cut(s) 266, 479
MaeI CTAG 1 cut(s) 527
MaeII ACGT 3 cut(s) 136, 277, 422
MalI GATC 3 cut(s) 237, 408, 461
MboI GATC 3 cut(s) 235, 406, 459
MboII GAAGA 2 cut(s) 3, 64
MluCI AATT 4 cut(s) 83, 352, 556, 602
MmeI TCCRAC 1 cut(s) 466
MnlI CCTC 8 cut(s) 55, 65, 68, 71, 100, 143, 150, 357
MroI TCCGGA 1 cut(s) 77
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 2 cut(s) 68, 616
MslI CAYNNNNRTG 2 cut(s) 262, 306
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 2 cut(s) 78, 113
MvnI CGCG 4 cut(s) 173, 188, 202, 218
MwoI GCNNNNNNNGC 6 cut(s) 131, 137, 194, 206, 215, 254
NdeII GATC 3 cut(s) 235, 406, 459
NlaIII CATG 1 cut(s) 442
NlaIV GGNNCC 2 cut(s) 338, 436
NmeAIII GCCGAG 1 cut(s) 387
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 2 cut(s) 223, 522
PkrI GCNGC 4 cut(s) 127, 130, 133, 199
Ple19I CGATCG 1 cut(s) 409
PmaCI CACGTG 1 cut(s) 137
PmlI CACGTG 1 cut(s) 137
Ppu21I YACGTR 2 cut(s) 137, 423
PshBI ATTAAT 1 cut(s) 68
PspCI CACGTG 1 cut(s) 137
PspN4I GGNNCC 2 cut(s) 338, 436
PspPI GGNCC 3 cut(s) 247, 267, 311
PvuI CGATCG 1 cut(s) 409
RsaI GTAC 2 cut(s) 338, 472
RsaNI GTAC 2 cut(s) 337, 471
RseI CAYNNNNRTG 2 cut(s) 262, 306
SacII CCGCGG 1 cut(s) 174
SaqAI TTAA 2 cut(s) 68, 616
SatI GCNGC 4 cut(s) 126, 129, 132, 198
Sau3AI GATC 3 cut(s) 235, 406, 459
Sau96I GGNCC 3 cut(s) 247, 267, 311
SetI ASST 8 cut(s) 139, 211, 280, 310, 338, 425, 550, 589
SfaNI GCATC 2 cut(s) 266, 479
Sfr303I CCGCGG 1 cut(s) 174
SgrBI CCGCGG 1 cut(s) 174
SmiMI CAYNNNNRTG 2 cut(s) 262, 306
Sse9I AATT 4 cut(s) 83, 352, 556, 602
SsiI CCGC 6 cut(s) 96, 126, 132, 171, 173, 444
SspI AATATT 1 cut(s) 328
SspMI CTAG 1 cut(s) 527
TaaI ACNGT 1 cut(s) 518
TaiI ACGT 3 cut(s) 139, 280, 425
TaqI TCGA 1 cut(s) 221
TasI AATT 4 cut(s) 83, 352, 556, 602
TauI GCSGC 2 cut(s) 128, 134
TfiI GAWTC 2 cut(s) 223, 522
Tru1I TTAA 2 cut(s) 68, 616
Tru9I TTAA 2 cut(s) 68, 616
TseI GCWGC 2 cut(s) 128, 197
TspDTI ATGAA 2 cut(s) 96, 405
TspGWI ACGGA 3 cut(s) 15, 37, 333
VspI ATTAAT 1 cut(s) 68
XapI RAATTY 1 cut(s) 83
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.