MD02G1191600.v1.1

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0019556
Physical Location & Seq
Reverse (-)
17875228 .. 17875749
522 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1191600.v1.1.491

Sequence Viewer

Length: 522 bp
ATGGGAAGCAGTGTAACAATTTTATTTACTTTCCTATGCATTAGCATATGTCTTGAGGCTACCATAACTCGTCATCATCCTATCCTTCGTGCTCCGACAGCGACCCCTCAGGAATTTTTGAAGGTTCACAACAATGTACGATGGAAAATTGGCCTACCGCCCTTGCAGTGGGACGAAAAACTAGCAGCGGTTGCTAGGGTTTACACGAAAACGAGAGCGACCATAGACTGCAGGATGGTACACTCGATGGGCCGTTATGGGGAGAACATCTTCTGGGGTGGCGGGAAACAACCGTGGGGGGCGAGGTTCGCGGTGAATTCGTGGTCACATGAGAAGTTGTTCTATGATTACAAGACCAATAGTTGCAAACCCGGGCAAATGTGTGGGCATTACACGCAAATGGTGTGGAAGGACACGAAGCGTCTGGGTTGTGCACGAGAGGTTTGCAGCAATGGAGCAGGGGAGCTCGTCATATGCAACTATGATCCACCTGGAAATTGGGCTGGAGAGAAACCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

174

Amino Acids

19.63

Weight (kDa)

9.16

Isoelectric Point (pI)

32.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 40 - 161 3.7e-20 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019556)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09590 AT5G02730
malus_domestica MD02G1191600.v1.1 MD15G1302700.v1.1
prunus_persica Prupe.6G214000_v2.0.a1
pyrus_communis pycom02g15640 pycom15g26600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 311
AciI CCGC 4 cut(s) 158, 188, 282, 311
AclWI GGATC 1 cut(s) 479
AcsI RAATTY 2 cut(s) 113, 316
AfaI GTAC 2 cut(s) 138, 240
AfiI CCNNNNNNNGG 2 cut(s) 168, 259
AgsI TTSAA 1 cut(s) 121
AjnI CCWGG 1 cut(s) 490
AluBI AGCT 1 cut(s) 466
AluI AGCT 1 cut(s) 466
Alw21I GWGCWC 3 cut(s) 94, 436, 468
Alw44I GTGCAC 1 cut(s) 432
AlwI GGATC 1 cut(s) 479
Ama87I CYCGRG 1 cut(s) 371
AoxI GGCC 2 cut(s) 151, 250
ApaLI GTGCAC 1 cut(s) 432
ApeKI GCWGC 2 cut(s) 185, 447
ApoI RAATTY 2 cut(s) 113, 316
Asp700I GAANNNNTTC 2 cut(s) 269, 338
AspS9I GGNCC 1 cut(s) 250
AsuC2I CCSGG 2 cut(s) 372, 373
AsuHPI GGTGA 1 cut(s) 325
AvaI CYCGRG 1 cut(s) 371
AxyI CCTNAGG 1 cut(s) 108
BaeGI GKGCMC 1 cut(s) 436
BanII GRGCYC 1 cut(s) 468
BauI CACGAG 1 cut(s) 435
Bbv12I GWGCWC 3 cut(s) 94, 436, 468
BbvI GCAGC 2 cut(s) 197, 459
BccI CCATC 3 cut(s) 135, 229, 241
BceAI ACGGC 1 cut(s) 237
BcgI CGANNNNNNTGC 2 cut(s) 426, 460
BciT130I CCWGG 1 cut(s) 492
BcnI CCSGG 2 cut(s) 372, 373
BfaI CTAG 2 cut(s) 182, 195
BfmI CTRYAG 1 cut(s) 229
BisI GCNGC 2 cut(s) 186, 448
BlsI GCNGC 2 cut(s) 187, 449
Bme1390I CCNGG 3 cut(s) 372, 373, 492
BmeT110I CYCGRG 1 cut(s) 371
BmgT120I GGNCC 1 cut(s) 250
BmrFI CCNGG 3 cut(s) 372, 373, 492
BpuEI CTTGAG 1 cut(s) 74
BpuMI CCSGG 2 cut(s) 372, 373
BsaJI CCNNGG 2 cut(s) 293, 371
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 259
Bse21I CCTNAGG 1 cut(s) 108
Bse3DI GCAATG 1 cut(s) 457
BseBI CCWGG 1 cut(s) 492
BseDI CCNNGG 2 cut(s) 293, 371
BseGI GGATG 2 cut(s) 76, 240
BseLI CCNNNNNNNGG 2 cut(s) 168, 259
BseMI GCAATG 1 cut(s) 457
BseMII CTCAG 1 cut(s) 122
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 2 cut(s) 197, 459
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 2 cut(s) 153, 252
BsiHKAI GWGCWC 3 cut(s) 94, 436, 468
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 1 cut(s) 372
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 2 cut(s) 168, 259
BsmFI GGGAC 1 cut(s) 185
BsnI GGCC 2 cut(s) 153, 252
BsoBI CYCGRG 1 cut(s) 371
Bsp1286I GDGCHC 3 cut(s) 94, 436, 468
Bsp143I GATC 1 cut(s) 484
BspACI CCGC 4 cut(s) 158, 188, 282, 311
BspANI GGCC 2 cut(s) 153, 252
BspCNI CTCAG 1 cut(s) 121
BspFNI CGCG 1 cut(s) 311
BspMAI CTGCAG 1 cut(s) 233
BspPI GGATC 1 cut(s) 479
BsrDI GCAATG 1 cut(s) 457
BssECI CCNNGG 2 cut(s) 293, 371
BssMI GATC 1 cut(s) 484
BssSI CACGAG 1 cut(s) 435
Bst2BI CACGAG 1 cut(s) 435
Bst2UI CCWGG 1 cut(s) 492
Bst4CI ACNGT 1 cut(s) 294
BstAPI GCANNNNNTGC 1 cut(s) 191
BstDEI CTNAG 1 cut(s) 108
BstDSI CCRYGG 1 cut(s) 293
BstF5I GGATG 2 cut(s) 76, 240
BstFNI CGCG 1 cut(s) 311
BstKTI GATC 1 cut(s) 487
BstMBI GATC 1 cut(s) 484
BstMWI GCNNNNNNNGC 4 cut(s) 98, 191, 308, 394
BstNI CCWGG 1 cut(s) 492
BstSCI CCNGG 3 cut(s) 370, 371, 490
BstSFI CTRYAG 1 cut(s) 229
BstSLI GKGCMC 1 cut(s) 436
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 2 cut(s) 197, 459
Bsu36I CCTNAGG 1 cut(s) 108
BsuRI GGCC 2 cut(s) 153, 252
BtgI CCRYGG 1 cut(s) 293
BtsCI GGATG 2 cut(s) 76, 240
BtsI GCAGTG 2 cut(s) 16, 173
BtsIMutI CAGTG 2 cut(s) 16, 173
Cfr13I GGNCC 1 cut(s) 250
Cfr9I CCCGGG 1 cut(s) 371
CseI GACGC 1 cut(s) 410
Csp6I GTAC 2 cut(s) 137, 239
CspCI CAANNNNNGTGG 2 cut(s) 386, 421
CviAII CATG 1 cut(s) 329
CviJI RGCY 5 cut(s) 59, 153, 252, 466, 503
CviKI_1 RGCY 5 cut(s) 59, 153, 252, 466, 503
CviQI GTAC 2 cut(s) 137, 239
DdeI CTNAG 1 cut(s) 108
DpnI GATC 1 cut(s) 486
DpnII GATC 1 cut(s) 484
Ecl136II GAGCTC 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 468
Eco53kI GAGCTC 1 cut(s) 466
Eco81I CCTNAGG 1 cut(s) 108
Eco88I CYCGRG 1 cut(s) 371
EcoICRI GAGCTC 1 cut(s) 466
EcoRI GAATTC 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 490
EcoT22I ATGCAT 1 cut(s) 41
EcoT38I GRGCYC 1 cut(s) 468
FaeI CATG 1 cut(s) 332
FaqI GGGAC 1 cut(s) 185
FatI CATG 1 cut(s) 328
FauI CCCGC 1 cut(s) 275
FauNDI CATATG 2 cut(s) 47, 473
Fnu4HI GCNGC 2 cut(s) 186, 448
FokI GGATG 2 cut(s) 63, 247
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 2 cut(s) 186, 448
FspBI CTAG 2 cut(s) 182, 195
GluI GCNGC 2 cut(s) 186, 448
HaeIII GGCC 2 cut(s) 153, 252
HapII CCGG 1 cut(s) 372
HgaI GACGC 1 cut(s) 410
Hin1II CATG 1 cut(s) 332
HpaII CCGG 1 cut(s) 372
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 4 cut(s) 127, 202, 241, 434
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 2 cut(s) 53, 110
Hpy8I GTNNAC 4 cut(s) 127, 202, 241, 434
HpyAV CCTTC 3 cut(s) 95, 115, 403
HpyCH4III ACNGT 1 cut(s) 294
HpyCH4V TGCA 7 cut(s) 39, 166, 231, 366, 434, 447, 477
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 191, 308, 394
HpyF3I CTNAG 1 cut(s) 108
Hsp92II CATG 1 cut(s) 332
Kzo9I GATC 1 cut(s) 484
LmnI GCTCC 3 cut(s) 97, 455, 463
LpnPI CCDG 9 cut(s) 95, 217, 259, 385, 410, 444, 477, 489, 504
Lsp1109I GCAGC 2 cut(s) 197, 459
MaeI CTAG 2 cut(s) 182, 195
MaeIII GTNAC 2 cut(s) 13, 324
MalI GATC 1 cut(s) 486
MboI GATC 1 cut(s) 484
MboII GAAGA 1 cut(s) 262
MhlI GDGCHC 3 cut(s) 94, 436, 468
MluCI AATT 5 cut(s) 18, 113, 147, 316, 496
MmeI TCCRAC 1 cut(s) 119
MnlI CCTC 4 cut(s) 49, 117, 297, 433
Mph1103I ATGCAT 1 cut(s) 41
MroXI GAANNNNTTC 2 cut(s) 269, 338
MslI CAYNNNNRTG 2 cut(s) 132, 398
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 3 cut(s) 372, 373, 492
MvaI CCWGG 1 cut(s) 492
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 4 cut(s) 98, 191, 308, 394
NciI CCSGG 2 cut(s) 372, 373
NdeI CATATG 2 cut(s) 47, 473
NdeII GATC 1 cut(s) 484
NlaIII CATG 1 cut(s) 332
NmuCI GTSAC 1 cut(s) 324
NsiI ATGCAT 1 cut(s) 41
PdmI GAANNNNTTC 2 cut(s) 269, 338
PkrI GCNGC 2 cut(s) 187, 449
Psp124BI GAGCTC 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 490
PspGI CCWGG 1 cut(s) 490
PspPI GGNCC 1 cut(s) 250
PstI CTGCAG 1 cut(s) 233
RsaI GTAC 2 cut(s) 138, 240
RsaNI GTAC 2 cut(s) 137, 239
RseI CAYNNNNRTG 2 cut(s) 132, 398
SacI GAGCTC 1 cut(s) 468
SatI GCNGC 2 cut(s) 186, 448
Sau3AI GATC 1 cut(s) 484
Sau96I GGNCC 1 cut(s) 250
ScrFI CCNGG 3 cut(s) 372, 373, 492
SduI GDGCHC 3 cut(s) 94, 436, 468
SetI ASST 6 cut(s) 126, 308, 444, 468, 493, 517
SfcI CTRYAG 1 cut(s) 229
SmaI CCCGGG 1 cut(s) 373
SmiMI CAYNNNNRTG 2 cut(s) 132, 398
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
Sse9I AATT 5 cut(s) 18, 113, 147, 316, 496
SsiI CCGC 4 cut(s) 158, 188, 282, 311
SspMI CTAG 2 cut(s) 182, 195
SstI GAGCTC 1 cut(s) 468
StyD4I CCNGG 3 cut(s) 370, 371, 490
TaaI ACNGT 1 cut(s) 294
TaqI TCGA 1 cut(s) 245
TasI AATT 5 cut(s) 18, 113, 147, 316, 496
TscAI CASTG 2 cut(s) 16, 173
TseFI GTSAC 1 cut(s) 324
TseI GCWGC 2 cut(s) 185, 447
Tsp45I GTSAC 1 cut(s) 324
TspMI CCCGGG 1 cut(s) 371
TspRI CASTG 2 cut(s) 16, 173
VneI GTGCAC 1 cut(s) 432
XapI RAATTY 2 cut(s) 113, 316
XcmI CCANNNNNNNNNTGG 1 cut(s) 495
XmaI CCCGGG 1 cut(s) 371
XmnI GAANNNNTTC 2 cut(s) 269, 338
XspI CTAG 2 cut(s) 182, 195
Zsp2I ATGCAT 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.