MD15G1302700.v1.1

Belongs to the CRISP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0019556
Physical Location & Seq
Reverse (-)
29303085 .. 29303655
571 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1302700.v1.1.491

Sequence Viewer

Length: 528 bp
ATGACGTCAATGGGAAGCCGAGTAACTATTTTAGCAACTATCGTATGCATTTTCATGTGTCTTGAGGCCGCACAAGCTCGTCATCATATTCGCCGTGCTCCGACAGCGAACCCTCATGATTTTCTGAAGGTTCACAACAATTTACGACGGAAAATTGGCCTACCGCCCTTGGTGTGGGACGAAAACCTAGCAGCGGTTGCTAGGATTTACACGAAACAGAGAGCGAGCATGGACTGCAGGATGGTACACTCAACGGGGCCTTACGGGGAGAACATCTTCTGGGGCGGCGGGAATCAGCCATGGGGGGCGAGGTTTGCGGCGAAGTCGTGGTCACACGAGAAGTTGTTCTATGATTACCAGACGGATAGTTGTAAAACCGGGCAGATGTGTGGGCATTATACGCAAATGGTGTGGAAGGACACGAAGCGTCTGGGTTGTGCACGAGAGGTTTGCAGAAATGGAGCCGGGGAGCTCATCATATGTAACTATGATCCGCCAGGAAATTGGGTTGGAGAGAAACCCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04626 map04016 map04075 map04626
Pfam Domains
Protein Families
CAP

Protein Analysis

176

Amino Acids

19.86

Weight (kDa)

9.19

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 42 - 163 7.4e-22 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019556)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09590 AT5G02730
malus_domestica MD02G1191600.v1.1 MD15G1302700.v1.1
prunus_persica Prupe.6G214000_v2.0.a1
pyrus_communis pycom02g15640 pycom15g26600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AciI CCGC 7 cut(s) 69, 164, 194, 285, 288, 317, 494
AclWI GGATC 1 cut(s) 485
AcuI CTGAAG 1 cut(s) 146
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 246
AfiI CCNNNNNNNGG 2 cut(s) 174, 193
AjnI CCWGG 1 cut(s) 496
AluBI AGCT 2 cut(s) 77, 472
AluI AGCT 2 cut(s) 77, 472
Alw21I GWGCWC 3 cut(s) 100, 442, 474
Alw44I GTGCAC 1 cut(s) 438
AlwI GGATC 1 cut(s) 485
AoxI GGCC 3 cut(s) 66, 157, 257
ApaLI GTGCAC 1 cut(s) 438
ApeKI GCWGC 1 cut(s) 191
ArsI GACNNNNNNTTYG 2 cut(s) 314, 346
Asp700I GAANNNNTTC 2 cut(s) 275, 344
AspS9I GGNCC 1 cut(s) 257
AsuC2I CCSGG 2 cut(s) 379, 466
BaeGI GKGCMC 1 cut(s) 442
BanII GRGCYC 1 cut(s) 474
BauI CACGAG 2 cut(s) 335, 441
Bbv12I GWGCWC 3 cut(s) 100, 442, 474
BbvI GCAGC 1 cut(s) 203
BccI CCATC 1 cut(s) 235
BceAI ACGGC 1 cut(s) 78
BcgI CGANNNNNNTGC 2 cut(s) 432, 466
BciT130I CCWGG 1 cut(s) 498
BcnI CCSGG 2 cut(s) 379, 466
BfaI CTAG 2 cut(s) 188, 201
BfmI CTRYAG 1 cut(s) 235
BisI GCNGC 4 cut(s) 69, 192, 286, 318
BlsI GCNGC 4 cut(s) 70, 193, 287, 319
Bme1390I CCNGG 3 cut(s) 379, 466, 498
BmgT120I GGNCC 1 cut(s) 257
BmiI GGNNCC 2 cut(s) 258, 463
BmrFI CCNGG 3 cut(s) 379, 466, 498
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 2 cut(s) 379, 466
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 168, 299, 465
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 193
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 3 cut(s) 168, 299, 465
BseGI GGATG 1 cut(s) 246
BseLI CCNNNNNNNGG 2 cut(s) 174, 193
BseSI GKGCMC 1 cut(s) 442
BseXI GCAGC 1 cut(s) 203
BshFI GGCC 3 cut(s) 68, 159, 259
BsiHKAI GWGCWC 3 cut(s) 100, 442, 474
BsiSI CCGG 2 cut(s) 378, 465
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 2 cut(s) 174, 193
BsmFI GGGAC 1 cut(s) 191
BsnI GGCC 3 cut(s) 68, 159, 259
Bsp1286I GDGCHC 3 cut(s) 100, 442, 474
Bsp143I GATC 1 cut(s) 490
Bsp19I CCATGG 1 cut(s) 299
BspACI CCGC 7 cut(s) 69, 164, 194, 285, 288, 317, 494
BspANI GGCC 3 cut(s) 68, 159, 259
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 2 cut(s) 258, 463
BspMAI CTGCAG 1 cut(s) 239
BspPI GGATC 1 cut(s) 485
BssECI CCNNGG 3 cut(s) 168, 299, 465
BssMI GATC 1 cut(s) 490
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 335, 441
BssT1I CCWWGG 2 cut(s) 168, 299
Bst2BI CACGAG 2 cut(s) 335, 441
Bst2UI CCWGG 1 cut(s) 498
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 2 cut(s) 197, 234
BstC8I GCNNGC 1 cut(s) 226
BstDSI CCRYGG 1 cut(s) 299
BstF5I GGATG 1 cut(s) 246
BstKTI GATC 1 cut(s) 493
BstMBI GATC 1 cut(s) 490
BstMWI GCNNNNNNNGC 6 cut(s) 74, 104, 197, 234, 314, 400
BstNI CCWGG 1 cut(s) 498
BstSCI CCNGG 3 cut(s) 377, 464, 496
BstSFI CTRYAG 1 cut(s) 235
BstSLI GKGCMC 1 cut(s) 442
BstV1I GCAGC 1 cut(s) 203
BstXI CCANNNNNNTGG 1 cut(s) 504
BsuRI GGCC 3 cut(s) 68, 159, 259
BtgI CCRYGG 1 cut(s) 299
BtsCI GGATG 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 226
CciI TCATGA 1 cut(s) 115
Cfr13I GGNCC 1 cut(s) 257
CseI GACGC 1 cut(s) 416
Csp6I GTAC 1 cut(s) 245
CspCI CAANNNNNGTGG 2 cut(s) 392, 427
CviAII CATG 4 cut(s) 55, 116, 229, 300
CviJI RGCY 8 cut(s) 18, 68, 77, 159, 259, 298, 464, 472
CviKI_1 RGCY 8 cut(s) 18, 68, 77, 159, 259, 298, 464, 472
CviQI GTAC 1 cut(s) 245
DpnI GATC 1 cut(s) 492
DpnII GATC 1 cut(s) 490
EciI GGCGGA 1 cut(s) 483
Ecl136II GAGCTC 1 cut(s) 472
Eco130I CCWWGG 2 cut(s) 168, 299
Eco24I GRGCYC 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 472
Eco57I CTGAAG 1 cut(s) 146
EcoICRI GAGCTC 1 cut(s) 472
EcoO109I RGGNCCY 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 496
EcoT14I CCWWGG 2 cut(s) 168, 299
EcoT22I ATGCAT 1 cut(s) 50
EcoT38I GRGCYC 1 cut(s) 474
ErhI CCWWGG 2 cut(s) 168, 299
FaeI CATG 4 cut(s) 58, 119, 232, 303
FaqI GGGAC 1 cut(s) 191
FatI CATG 4 cut(s) 54, 115, 228, 299
FauI CCCGC 1 cut(s) 281
FauNDI CATATG 1 cut(s) 479
Fnu4HI GCNGC 4 cut(s) 69, 192, 286, 318
FokI GGATG 1 cut(s) 253
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 4 cut(s) 69, 192, 286, 318
FspBI CTAG 2 cut(s) 188, 201
GluI GCNGC 4 cut(s) 69, 192, 286, 318
HaeIII GGCC 3 cut(s) 68, 159, 259
HapII CCGG 2 cut(s) 378, 465
HgaI GACGC 1 cut(s) 416
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 58, 119, 232, 303
HinfI GANTC 1 cut(s) 292
HpaII CCGG 2 cut(s) 378, 465
Hpy166II GTNNAC 3 cut(s) 133, 247, 440
Hpy188I TCNGA 2 cut(s) 102, 126
Hpy188III TCNNGA 2 cut(s) 62, 116
Hpy8I GTNNAC 3 cut(s) 133, 247, 440
Hpy99I CGWCG 1 cut(s) 150
HpyAV CCTTC 2 cut(s) 121, 409
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 4 cut(s) 48, 237, 440, 453
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 104, 197, 234, 314, 400
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 58, 119, 232, 303
Kzo9I GATC 1 cut(s) 490
LmnI GCTCC 3 cut(s) 103, 461, 469
LpnPI CCDG 8 cut(s) 223, 265, 371, 391, 416, 478, 483, 510
Lsp1109I GCAGC 1 cut(s) 203
MaeI CTAG 2 cut(s) 188, 201
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 3 cut(s) 22, 330, 482
MalI GATC 1 cut(s) 492
MboI GATC 1 cut(s) 490
MboII GAAGA 1 cut(s) 268
MhlI GDGCHC 3 cut(s) 100, 442, 474
MluCI AATT 3 cut(s) 139, 153, 502
MmeI TCCRAC 2 cut(s) 125, 490
MnlI CCTC 4 cut(s) 58, 123, 303, 439
Mph1103I ATGCAT 1 cut(s) 50
MroXI GAANNNNTTC 2 cut(s) 275, 344
MslI CAYNNNNRTG 1 cut(s) 53
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 2 cut(s) 378, 465
MspR9I CCNGG 3 cut(s) 379, 466, 498
MvaI CCWGG 1 cut(s) 498
MwoI GCNNNNNNNGC 6 cut(s) 74, 104, 197, 234, 314, 400
NciI CCSGG 2 cut(s) 379, 466
NcoI CCATGG 1 cut(s) 299
NdeI CATATG 1 cut(s) 479
NdeII GATC 1 cut(s) 490
NlaIII CATG 4 cut(s) 58, 119, 232, 303
NlaIV GGNNCC 2 cut(s) 258, 463
NmeAIII GCCGAG 1 cut(s) 44
NmuCI GTSAC 1 cut(s) 330
NsiI ATGCAT 1 cut(s) 50
PagI TCATGA 1 cut(s) 115
PdmI GAANNNNTTC 2 cut(s) 275, 344
PfeI GAWTC 1 cut(s) 292
PkrI GCNGC 4 cut(s) 70, 193, 287, 319
Psp124BI GAGCTC 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 496
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 2 cut(s) 258, 463
PspPI GGNCC 1 cut(s) 257
PstI CTGCAG 1 cut(s) 239
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
RseI CAYNNNNRTG 1 cut(s) 53
SacI GAGCTC 1 cut(s) 474
SatI GCNGC 4 cut(s) 69, 192, 286, 318
Sau3AI GATC 1 cut(s) 490
Sau96I GGNCC 1 cut(s) 257
ScrFI CCNGG 3 cut(s) 379, 466, 498
SduI GDGCHC 3 cut(s) 100, 442, 474
SetI ASST 7 cut(s) 8, 79, 132, 189, 314, 450, 474
SfcI CTRYAG 1 cut(s) 235
SmiMI CAYNNNNRTG 1 cut(s) 53
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 3 cut(s) 139, 153, 502
SsiI CCGC 7 cut(s) 69, 164, 194, 285, 288, 317, 494
SspMI CTAG 2 cut(s) 188, 201
SstI GAGCTC 1 cut(s) 474
StyD4I CCNGG 3 cut(s) 377, 464, 496
StyI CCWWGG 2 cut(s) 168, 299
TaiI ACGT 1 cut(s) 8
TasI AATT 3 cut(s) 139, 153, 502
TauI GCSGC 3 cut(s) 71, 288, 320
TfiI GAWTC 1 cut(s) 292
TseFI GTSAC 1 cut(s) 330
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 330
TspDTI ATGAA 1 cut(s) 43
TspGWI ACGGA 2 cut(s) 163, 377
VneI GTGCAC 1 cut(s) 438
XmnI GAANNNNTTC 2 cut(s) 275, 344
XspI CTAG 2 cut(s) 188, 201
ZraI GACGTC 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.