AT5G04200

Caspase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016675
Physical Location & Seq
Forward (+)
1153740 .. 1155040
1301 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04200.1

Sequence Viewer

Length: 978 bp
ATGGATCAACAAGGGATGGTCAAGAAAAGACTAGCCGTTCTCGTCGGCTGCAACTACCCTAACACAAGGAACGAGCTTCACGGTTGCATCAATGATGTTCTAGCCATGAAAGAAACTATTTTGTCACGTTTCGGATTCAAACAAGACGACATTGAGGTGCTAACGGACGAGCCAGAATCAAAGGTAAAGCCGACAGGTGCCAACATCAAGGCTGCTCTACGTAGGATGGTGGATAAAGCTCAAGCCGGATCTGGAGACATCTTGTTCTTCCACTACAGTGGCCATGGAACTCGTATTCCTTCGGTTAAATCTGCTCATCCTTTTAAACAAGACGAAGCTATTGTTCCTTGTGATTTCAATCTCATCACAGATGTTGATTTCCGAGAATTGGTCAATCAACTTCCTAAAGGAACAAGCTTTACCATGATCTCTGATTCTTGCCATAGTGGTGGTCTCATCGACAAGGAGAAAGAACAGATCGGACCCTCTTCGGTCTCCAGCAACATATCTCCGGCTATTGAGACAACGAACAAGACTATAACATCACGTGCCTTGCCCTTCAAGGCAGTCCTTGACCACTTGAGTTCTCTCACCGGAATTACAACTTCCGACATCGGCACACATCTACTTGAGCTCTTTGGCCGCGATGCTGGTCTTAAGTTTCGGTTACCGGCAATGGATCTAATGGATCTTTTGGAGACTATGACGGCCCGTGAAAAACACGTGGACTCAGGGATATTGATGAGCGGGTGTCAGGCGGATGAGACGTCTGCTGACGTCGGCGTTGGGAACGGGAAAGCGTACGGAGCGTTTAGCAACGCGATTCAAAGGGTGTTGAACGAAAATGAAGGTGCCATGAAGAACAAGCAACTAGTGATGATGGCAAGGGATGTGCTTGAGAGACTAGGGTTTCATCAACACCCTTGTCTCTACTGCAGCGACCAGAACGCAGACGCGACGTTCCTTTCTCAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016787 GO:0019538 GO:0043170 GO:0044238 GO:0048046 GO:0070011 GO:0071704 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C14

Protein Analysis

325

Amino Acids

35.51

Weight (kDa)

5.81

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C14 PF00656 9 - 316 1.2e-42 Caspase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016675)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04200
fragaria_vesca FvH4_3g29720
malus_domestica MD03G1136900.v1.1
prunus_persica Prupe.6G122000_v2.0.a1
pyrus_communis pycom03g09280
rosa_chinensis RchiOBHm_Chr5g0055101
rosa_laevigata RLG00000034964
rosa_multiflora Rmu_sc0001694.1_g000018
rosa_roxburghii Rroxscaffold_1G00025350
rosa_samantha Rh5AG360800 Rh5BG371900 Rh5CG394000 Rh5DG385600
rosa_wichuraiana Rw5G033930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 770, 780
AccB1I GGYRCC 2 cut(s) 197, 851
AccBSI CCGCTC 1 cut(s) 747
AccII CGCG 3 cut(s) 645, 821, 956
AciI CCGC 3 cut(s) 643, 747, 758
AclWI GGATC 4 cut(s) 12, 256, 687, 696
AcoI YGGCCR 2 cut(s) 280, 640
AcvI CACGTG 2 cut(s) 548, 724
AcyI GRCGYC 2 cut(s) 767, 777
AfaI GTAC 1 cut(s) 803
AfiI CCNNNNNNNGG 1 cut(s) 388
AflII CTTAAG 1 cut(s) 656
AflIII ACRYGT 1 cut(s) 721
AgsI TTSAA 5 cut(s) 139, 358, 562, 827, 838
AhlI ACTAGT 1 cut(s) 871
AleI CACNNNNGTG 1 cut(s) 276
AloI GAACNNNNNNTCC 2 cut(s) 280, 312
AluBI AGCT 5 cut(s) 76, 239, 338, 417, 634
AluI AGCT 5 cut(s) 76, 239, 338, 417, 634
Alw21I GWGCWC 1 cut(s) 636
Alw26I GTCTC 8 cut(s) 249, 458, 499, 515, 692, 758, 895, 932
AlwI GGATC 4 cut(s) 12, 256, 687, 696
AoxI GGCC 3 cut(s) 280, 640, 708
ApeKI GCWGC 3 cut(s) 48, 212, 936
AspS9I GGNCC 2 cut(s) 482, 709
AsuHPI GGTGA 1 cut(s) 583
AvaII GGWCC 1 cut(s) 482
BalI TGGCCA 1 cut(s) 282
BanI GGYRCC 2 cut(s) 197, 851
BanII GRGCYC 1 cut(s) 636
BbrPI CACGTG 2 cut(s) 548, 724
Bbv12I GWGCWC 1 cut(s) 636
BbvI GCAGC 3 cut(s) 35, 199, 948
BccI CCATC 3 cut(s) 10, 220, 874
BceAI ACGGC 2 cut(s) 20, 723
BcoDI GTCTC 8 cut(s) 249, 458, 499, 515, 692, 758, 895, 932
BcuI ACTAGT 1 cut(s) 871
BfaI CTAG 4 cut(s) 32, 101, 872, 905
BfmI CTRYAG 2 cut(s) 274, 934
BfrI CTTAAG 1 cut(s) 656
BisI GCNGC 4 cut(s) 49, 213, 643, 937
BlsI GCNGC 4 cut(s) 50, 214, 644, 938
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 2 cut(s) 482, 709
BmiI GGNNCC 3 cut(s) 199, 484, 853
BmsI GCATC 2 cut(s) 96, 637
BpmI CTGGAG 2 cut(s) 273, 481
BpuEI CTTGAG 4 cut(s) 225, 601, 650, 917
BsaAI YACGTR 3 cut(s) 221, 548, 724
BsaBI GATNNNNATC 1 cut(s) 357
BsaHI GRCGYC 2 cut(s) 767, 777
BsaI GGTCTC 2 cut(s) 458, 499
BsaJI CCNNGG 1 cut(s) 283
BsaWI WCCGGW 1 cut(s) 593
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse118I RCCGGY 1 cut(s) 670
Bse3DI GCAATG 1 cut(s) 681
Bse8I GATNNNNATC 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 5 cut(s) 21, 231, 316, 766, 895
BseJI GATNNNNATC 1 cut(s) 357
BseLI CCNNNNNNNGG 1 cut(s) 388
BseMI GCAATG 1 cut(s) 681
BseMII CTCAG 1 cut(s) 744
BseXI GCAGC 3 cut(s) 35, 199, 948
Bsh1236I CGCG 3 cut(s) 645, 821, 956
BshFI GGCC 3 cut(s) 282, 642, 710
BshNI GGYRCC 2 cut(s) 197, 851
BsiHKAI GWGCWC 1 cut(s) 636
BsiSI CCGG 4 cut(s) 246, 512, 594, 671
BsiWI CGTACG 1 cut(s) 801
BslI CCNNNNNNNGG 1 cut(s) 388
BsmAI GTCTC 8 cut(s) 249, 458, 499, 515, 692, 758, 895, 932
BsmBI CGTCTC 1 cut(s) 758
BsnI GGCC 3 cut(s) 282, 642, 710
Bso31I GGTCTC 2 cut(s) 458, 499
Bsp1286I GDGCHC 1 cut(s) 636
Bsp143I GATC 6 cut(s) 4, 248, 426, 477, 679, 688
Bsp19I CCATGG 1 cut(s) 283
BspACI CCGC 3 cut(s) 643, 747, 758
BspANI GGCC 3 cut(s) 282, 642, 710
BspCNI CTCAG 1 cut(s) 743
BspFNI CGCG 3 cut(s) 645, 821, 956
BspLI GGNNCC 3 cut(s) 199, 484, 853
BspMAI CTGCAG 1 cut(s) 938
BspPI GGATC 4 cut(s) 12, 256, 687, 696
BspT107I GGYRCC 2 cut(s) 197, 851
BspTI CTTAAG 1 cut(s) 656
BspTNI GGTCTC 2 cut(s) 458, 499
BsrBI CCGCTC 1 cut(s) 747
BsrDI GCAATG 1 cut(s) 681
BsrFI RCCGGY 1 cut(s) 670
BssAI RCCGGY 1 cut(s) 670
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 6 cut(s) 4, 248, 426, 477, 679, 688
BssNI GRCGYC 2 cut(s) 767, 777
BssT1I CCWWGG 1 cut(s) 283
Bst4CI ACNGT 2 cut(s) 83, 278
Bst6I CTCTTC 1 cut(s) 493
BstACI GRCGYC 2 cut(s) 767, 777
BstAFI CTTAAG 1 cut(s) 656
BstBAI YACGTR 3 cut(s) 221, 548, 724
BstDEI CTNAG 1 cut(s) 730
BstDSI CCRYGG 1 cut(s) 283
BstEII GGTNACC 1 cut(s) 666
BstF5I GGATG 5 cut(s) 21, 231, 316, 766, 895
BstFNI CGCG 3 cut(s) 645, 821, 956
BstKTI GATC 6 cut(s) 7, 251, 429, 480, 682, 691
BstMAI GTCTC 8 cut(s) 249, 458, 499, 515, 692, 758, 895, 932
BstMBI GATC 6 cut(s) 4, 248, 426, 477, 679, 688
BstMWI GCNNNNNNNGC 1 cut(s) 806
BstPI GGTNACC 1 cut(s) 666
BstSFI CTRYAG 2 cut(s) 274, 934
BstSNI TACGTA 1 cut(s) 221
BstUI CGCG 3 cut(s) 645, 821, 956
BstV1I GCAGC 3 cut(s) 35, 199, 948
BstX2I RGATCY 3 cut(s) 248, 679, 688
BstXI CCANNNNNNTGG 2 cut(s) 278, 449
BstYI RGATCY 3 cut(s) 248, 679, 688
BsuRI GGCC 3 cut(s) 282, 642, 710
BtgI CCRYGG 1 cut(s) 283
BtgZI GCGATG 1 cut(s) 660
BtsCI GGATG 5 cut(s) 21, 231, 316, 766, 895
BtsIMutI CAGTG 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 670
Cfr13I GGNCC 2 cut(s) 482, 709
CseI GACGC 1 cut(s) 962
Csp6I GTAC 1 cut(s) 802
CviAII CATG 4 cut(s) 106, 284, 424, 856
CviQI GTAC 1 cut(s) 802
DdeI CTNAG 1 cut(s) 730
DpnI GATC 6 cut(s) 6, 250, 428, 479, 681, 690
DpnII GATC 6 cut(s) 4, 248, 426, 477, 679, 688
DraI TTTAAA 1 cut(s) 325
EaeI YGGCCR 2 cut(s) 280, 640
Eam1104I CTCTTC 1 cut(s) 493
EarI CTCTTC 1 cut(s) 493
EciI GGCGGA 1 cut(s) 773
Ecl136II GAGCTC 1 cut(s) 634
Eco105I TACGTA 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 636
Eco31I GGTCTC 2 cut(s) 458, 499
Eco47I GGWCC 1 cut(s) 482
Eco53kI GAGCTC 1 cut(s) 634
Eco72I CACGTG 2 cut(s) 548, 724
Eco91I GGTNACC 1 cut(s) 666
EcoICRI GAGCTC 1 cut(s) 634
EcoO65I GGTNACC 1 cut(s) 666
EcoT14I CCWWGG 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 636
ErhI CCWWGG 1 cut(s) 283
Esp3I CGTCTC 1 cut(s) 758
FaeI CATG 4 cut(s) 109, 287, 427, 859
FaiI YATR 8 cut(s) 107, 285, 425, 444, 506, 539, 704, 857
FatI CATG 4 cut(s) 105, 283, 423, 855
FauI CCCGC 1 cut(s) 740
Fnu4HI GCNGC 4 cut(s) 49, 213, 643, 937
FokI GGATG 5 cut(s) 28, 238, 303, 773, 902
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 4 cut(s) 49, 213, 643, 937
FspBI CTAG 4 cut(s) 32, 101, 872, 905
GluI GCNGC 4 cut(s) 49, 213, 643, 937
GsuI CTGGAG 2 cut(s) 273, 481
HaeIII GGCC 3 cut(s) 282, 642, 710
HapII CCGG 4 cut(s) 246, 512, 594, 671
HgaI GACGC 1 cut(s) 962
Hin1I GRCGYC 2 cut(s) 767, 777
Hin1II CATG 4 cut(s) 109, 287, 427, 859
HindIII AAGCTT 1 cut(s) 415
HinfI GANTC 5 cut(s) 135, 176, 434, 728, 823
HpaII CCGG 4 cut(s) 246, 512, 594, 671
HphI GGTGA 1 cut(s) 583
Hpy166II GTNNAC 1 cut(s) 727
Hpy188I TCNGA 5 cut(s) 134, 383, 433, 482, 610
Hpy188III TCNNGA 2 cut(s) 22, 252
Hpy8I GTNNAC 1 cut(s) 727
Hpy99I CGWCG 3 cut(s) 47, 782, 961
HpyAV CCTTC 3 cut(s) 309, 568, 842
HpyCH4III ACNGT 2 cut(s) 83, 278
HpyCH4IV ACGT 7 cut(s) 127, 220, 547, 723, 767, 777, 959
HpyCH4V TGCA 3 cut(s) 51, 87, 936
HpyF10VI GCNNNNNNNGC 1 cut(s) 806
HpyF3I CTNAG 1 cut(s) 730
HpySE526I ACGT 7 cut(s) 127, 220, 547, 723, 767, 777, 959
Hsp92I GRCGYC 2 cut(s) 767, 777
Hsp92II CATG 4 cut(s) 109, 287, 427, 859
Kzo9I GATC 6 cut(s) 4, 248, 426, 477, 679, 688
LmnI GCTCC 1 cut(s) 806
Lsp1109I GCAGC 3 cut(s) 35, 199, 948
LweI GCATC 2 cut(s) 96, 637
MaeI CTAG 4 cut(s) 32, 101, 872, 905
MaeII ACGT 7 cut(s) 127, 220, 547, 723, 767, 777, 959
MaeIII GTNAC 2 cut(s) 123, 666
MalI GATC 6 cut(s) 6, 250, 428, 479, 681, 690
MbiI CCGCTC 1 cut(s) 747
MboI GATC 6 cut(s) 4, 248, 426, 477, 679, 688
MboII GAAGA 3 cut(s) 259, 480, 871
MflI RGATCY 3 cut(s) 248, 679, 688
MhlI GDGCHC 1 cut(s) 636
MlsI TGGCCA 1 cut(s) 282
MluCI AATT 2 cut(s) 386, 597
MluNI TGGCCA 1 cut(s) 282
MlyI GAGTC 1 cut(s) 722
MmeI TCCRAC 1 cut(s) 633
MnlI CCTC 2 cut(s) 148, 496
Mox20I TGGCCA 1 cut(s) 282
MscI TGGCCA 1 cut(s) 282
MseI TTAA 3 cut(s) 306, 324, 657
MslI CAYNNNNRTG 3 cut(s) 155, 276, 447
Msp20I TGGCCA 1 cut(s) 282
MspCI CTTAAG 1 cut(s) 656
MspI CCGG 4 cut(s) 246, 512, 594, 671
MvnI CGCG 3 cut(s) 645, 821, 956
MwoI GCNNNNNNNGC 1 cut(s) 806
NcoI CCATGG 1 cut(s) 283
NdeII GATC 6 cut(s) 4, 248, 426, 477, 679, 688
NlaIII CATG 4 cut(s) 109, 287, 427, 859
NlaIV GGNNCC 3 cut(s) 199, 484, 853
NmuCI GTSAC 1 cut(s) 123
OliI CACNNNNGTG 1 cut(s) 276
PfeI GAWTC 4 cut(s) 135, 176, 434, 823
Pfl23II CGTACG 1 cut(s) 801
PkrI GCNGC 4 cut(s) 50, 214, 644, 938
PleI GAGTC 1 cut(s) 722
PmaCI CACGTG 2 cut(s) 548, 724
PmlI CACGTG 2 cut(s) 548, 724
PpsI GAGTC 1 cut(s) 722
Ppu21I YACGTR 3 cut(s) 221, 548, 724
Psp124BI GAGCTC 1 cut(s) 636
PspCI CACGTG 2 cut(s) 548, 724
PspEI GGTNACC 1 cut(s) 666
PspLI CGTACG 1 cut(s) 801
PspN4I GGNNCC 3 cut(s) 199, 484, 853
PspPI GGNCC 2 cut(s) 482, 709
PsrI GAACNNNNNNTAC 2 cut(s) 403, 435
PstI CTGCAG 1 cut(s) 938
PsuI RGATCY 3 cut(s) 248, 679, 688
RsaI GTAC 1 cut(s) 803
RsaNI GTAC 1 cut(s) 802
RseI CAYNNNNRTG 3 cut(s) 155, 276, 447
SacI GAGCTC 1 cut(s) 636
SaqAI TTAA 3 cut(s) 306, 324, 657
SatI GCNGC 4 cut(s) 49, 213, 643, 937
Sau3AI GATC 6 cut(s) 4, 248, 426, 477, 679, 688
Sau96I GGNCC 2 cut(s) 482, 709
SchI GAGTC 1 cut(s) 722
SduI GDGCHC 1 cut(s) 636
SfaNI GCATC 2 cut(s) 96, 637
SfcI CTRYAG 2 cut(s) 274, 934
SinI GGWCC 1 cut(s) 482
SmiMI CAYNNNNRTG 3 cut(s) 155, 276, 447
SmlI CTYRAG 5 cut(s) 240, 580, 629, 656, 896
SmoI CTYRAG 5 cut(s) 240, 580, 629, 656, 896
SnaBI TACGTA 1 cut(s) 221
SpeI ACTAGT 1 cut(s) 871
Sse9I AATT 2 cut(s) 386, 597
SsiI CCGC 3 cut(s) 643, 747, 758
SspMI CTAG 4 cut(s) 32, 101, 872, 905
SstI GAGCTC 1 cut(s) 636
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 2 cut(s) 83, 278
TaiI ACGT 7 cut(s) 130, 223, 550, 726, 770, 780, 962
TaqI TCGA 1 cut(s) 459
TaqII GACCGA 1 cut(s) 481
TasI AATT 2 cut(s) 386, 597
TauI GCSGC 1 cut(s) 645
TfiI GAWTC 4 cut(s) 135, 176, 434, 823
Tru1I TTAA 3 cut(s) 306, 324, 657
Tru9I TTAA 3 cut(s) 306, 324, 657
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 1 cut(s) 123
TseI GCWGC 3 cut(s) 48, 212, 936
Tsp45I GTSAC 1 cut(s) 123
TspDTI ATGAA 4 cut(s) 122, 861, 872, 902
TspGWI ACGGA 2 cut(s) 179, 819
TspRI CASTG 1 cut(s) 283
Vha464I CTTAAG 1 cut(s) 656
VpaK11BI GGWCC 1 cut(s) 482
XspI CTAG 4 cut(s) 32, 101, 872, 905
ZraI GACGTC 2 cut(s) 768, 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.