AT5G06560

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007394
Physical Location & Seq
Reverse (-)
2003304 .. 2006347
3044 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G06560.1

Sequence Viewer

Length: 1557 bp
ATGGATTTGGCAATTGTATCTTCCCCTACTAGGGATGTGGTTAGATGTTGTGATTGTGGATGTGATTGCTCTCTGAATGGTGCTTCTCCTGGTTCTTTGCTTCGGTCTGTGAAGAGAAAATACGAGGAATTTGAGAATGAGAAACTGTTTCACATACCGGAGCTGGAGCTGGACTTGTCTTCCAATGCTAAAGTTCAAATTGAGAATGAATTGGAGCTGCTTAGGGAGACTGTTAGCAGTCAACAGCAGTCGATTCAAGATTTGTATGAGGAGCTTGATGAAGAGCGGAATGCTGCTTCGACAGCTGCAAGTGAGGCAATGTCTATGATATTGAGGTTGCAGAGGGATAAAGCTGAACTCCAAATGGAATTGAGACAGTTTAAGCGGTTTGCTGAGGAGAAAATGGAGCATGATCAACAAGAGCTTTTGGATCTGGAAGATTTGATATATAAGAGGGAGCAGACTATTCAGGCTTTGACATTTGAAGCTCAGGCTTATAAGCATAGAATGATGAGTTTTGGGTTCACTGAGGCTGAGGTTGAAACGGAGAAGAACATGCTTAGCCGTAATCCGAGCATGATCGAAAATGATTACCAATATGATCTCCCCACATCTGATTACCCTCCTATAAAGTGCAATGTGAATGAGAACCCAGGTCCCCTGGAGGCTGATATTGATGTTGATGATGTTGAAAAGTACCCATTGGCTGATTCACCTCACCCATTGAAGACTTTGGAACGAAGGATAAGTCAGATGGAGAGAAATCCTAGCTTTACTCAGCCTACTGGTGATGTATCTGGAGGGAGACATTATACAGAGAAGAACGTGGTTGGTCAGAGTCCTAGGCATCAGAGACATTTCAGGAGGGTTTCCACTGGTAGTGCAAGTTCACTTTTGGGAACAACTAGAGAAAAAAGGCTTGATTTTTCTAATGATTCCCCAAGGTCCAACAATGGTAGCTTCAGGAAAATGGAAGATCCCCCATATGCAGCTGGTAATTCCTTTGCAAGGGATAAGGGTGACTCATCGGAAATAGGAGATAATGACATGAATGATAGAGTTTATACCATTGACTCTGTCCATCACAGTGTATCTCATAGTGGTACTGCTGAGCAAAAGTTTAAGAATGACACTGCTGATGGTTATGCAATGTCTCCGAGAGAAATATCGAATCAGCCGGATCTTGGTGATCCTGAAATATCGAAACTTTACATGAGACTTCAAGCACTAGAGGCTGACAGGGAATCAATGAGACAGGCGATTATGTCGATGAGGACTGAGAAAGCACAGATGGTTCTGTTGAAAGAAATTGCCCAACATCTGTCAAAGGATGTTGTCCCAGAACGTAGATTGCCTCTGCGGAAAACATCTATTATTGGAGCATTCAACTTCATATCTGTCTTTAAGTGGATCACATCTTTTGTTTTCTGGAGAAGAAAGGCTCGTCGAAGCAAATACATGAACGGGGTACAAGGGAACAACATGGGATTGCAAATGCTTCTAGAAAAGACTCCTCGAATACGGCAATGGAGATGTCTCTCAAGCACACAAGTGTGA

Protein Analysis

518

Amino Acids

59.43

Weight (kDa)

5.59

Isoelectric Point (pI)

56.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 73 - 162 5e-34 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 498
AccBSI CCGCTC 1 cut(s) 286
AciI CCGC 3 cut(s) 286, 385, 1360
AclWI GGATC 5 cut(s) 438, 971, 1184, 1188, 1418
AcsI RAATTY 1 cut(s) 128
AcuI CTGAAG 1 cut(s) 946
AfaI GTAC 3 cut(s) 698, 1105, 1470
AfiI CCNNNNNNNGG 4 cut(s) 30, 31, 1008, 1184
AgsI TTSAA 9 cut(s) 197, 257, 485, 542, 692, 727, 1223, 1303, 1387
AjnI CCWGG 3 cut(s) 88, 652, 660
AleI CACNNNNGTG 2 cut(s) 1550, 1552
Alw26I GTCTC 8 cut(s) 221, 367, 799, 847, 1158, 1210, 1246, 1541
AlwI GGATC 5 cut(s) 438, 971, 1184, 1188, 1418
ApeKI GCWGC 4 cut(s) 217, 293, 305, 989
ApoI RAATTY 1 cut(s) 128
ArsI GACNNNNNNTTYG 2 cut(s) 733, 765
AspA2I CCTAGG 1 cut(s) 842
AspS9I GGNCC 2 cut(s) 656, 945
AsuHPI GGTGA 5 cut(s) 705, 710, 800, 1031, 1199
AvaII GGWCC 2 cut(s) 656, 945
AvrII CCTAGG 1 cut(s) 842
BarI GAAGNNNNNNTAC 2 cut(s) 104, 136
BbsI GAAGAC 2 cut(s) 171, 734
BbvCI CCTCAGC 2 cut(s) 393, 534
BbvI GCAGC 4 cut(s) 204, 280, 292, 1001
BccI CCATC 4 cut(s) 748, 1089, 1133, 1285
BceAI ACGGC 2 cut(s) 549, 1538
BciT130I CCWGG 3 cut(s) 90, 654, 662
BclI TGATCA 1 cut(s) 412
BcoDI GTCTC 8 cut(s) 221, 367, 799, 847, 1158, 1210, 1246, 1541
BfaI CTAG 6 cut(s) 30, 768, 843, 906, 1229, 1502
BisI GCNGC 4 cut(s) 218, 294, 306, 990
BlnI CCTAGG 1 cut(s) 842
BlpI GCTNAGC 2 cut(s) 560, 1110
BlsI GCNGC 4 cut(s) 219, 295, 307, 991
Bme1390I CCNGG 3 cut(s) 90, 654, 662
Bme18I GGWCC 2 cut(s) 656, 945
BmgT120I GGNCC 2 cut(s) 656, 945
BmiI GGNNCC 1 cut(s) 658
BmrFI CCNGG 3 cut(s) 90, 654, 662
BmsI GCATC 1 cut(s) 856
BpiI GAAGAC 2 cut(s) 171, 734
BpmI CTGGAG 4 cut(s) 185, 683, 819, 1450
Bpu10I CCTNAGC 4 cut(s) 221, 393, 489, 534
Bpu1102I GCTNAGC 2 cut(s) 560, 1110
BpuEI CTTGAG 1 cut(s) 1525
BsaJI CCNNGG 4 cut(s) 652, 660, 842, 941
BsaWI WCCGGW 1 cut(s) 157
Bsc4I CCNNNNNNNGG 4 cut(s) 30, 31, 1008, 1184
Bse1I ACTGG 2 cut(s) 790, 880
Bse3DI GCAATG 4 cut(s) 324, 643, 1155, 1532
BseBI CCWGG 3 cut(s) 90, 654, 662
BseDI CCNNGG 4 cut(s) 652, 660, 842, 941
BseGI GGATG 3 cut(s) 40, 65, 1336
BseLI CCNNNNNNNGG 4 cut(s) 30, 31, 1008, 1184
BseMI GCAATG 4 cut(s) 324, 643, 1155, 1532
BseMII CTCAG 7 cut(s) 384, 503, 519, 525, 791, 1101, 1269
BseNI ACTGG 2 cut(s) 790, 880
BseRI GAGGAG 3 cut(s) 284, 410, 1503
BseXI GCAGC 4 cut(s) 204, 280, 292, 1001
BsiSI CCGG 2 cut(s) 158, 1178
BslFI GGGAC 2 cut(s) 642, 1322
BslI CCNNNNNNNGG 4 cut(s) 30, 31, 1008, 1184
BsmAI GTCTC 8 cut(s) 221, 367, 799, 847, 1158, 1210, 1246, 1541
BsmFI GGGAC 2 cut(s) 642, 1322
BsmI GAATGC 2 cut(s) 295, 1382
Bsp143I GATC 8 cut(s) 412, 430, 579, 601, 976, 1180, 1189, 1410
Bsp1720I GCTNAGC 2 cut(s) 560, 1110
BspACI CCGC 3 cut(s) 286, 385, 1360
BspCNI CTCAG 7 cut(s) 385, 502, 520, 526, 790, 1102, 1270
BspLI GGNNCC 1 cut(s) 658
BspPI GGATC 5 cut(s) 438, 971, 1184, 1188, 1418
BspQI GCTCTTC 1 cut(s) 276
BsrBI CCGCTC 1 cut(s) 286
BsrDI GCAATG 4 cut(s) 324, 643, 1155, 1532
BsrI ACTGG 2 cut(s) 790, 880
BssECI CCNNGG 4 cut(s) 652, 660, 842, 941
BssMI GATC 8 cut(s) 412, 430, 579, 601, 976, 1180, 1189, 1410
BssT1I CCWWGG 2 cut(s) 842, 941
Bst2UI CCWGG 3 cut(s) 90, 654, 662
Bst4CI ACNGT 4 cut(s) 147, 232, 378, 1088
Bst6I CTCTTC 2 cut(s) 107, 276
BstDEI CTNAG 9 cut(s) 221, 393, 489, 528, 534, 560, 777, 1110, 1278
BstENI CCTNNNNNAGG 1 cut(s) 1006
BstF5I GGATG 3 cut(s) 40, 65, 1336
BstKTI GATC 8 cut(s) 415, 433, 582, 604, 979, 1183, 1192, 1413
BstMAI GTCTC 8 cut(s) 221, 367, 799, 847, 1158, 1210, 1246, 1541
BstMBI GATC 8 cut(s) 412, 430, 579, 601, 976, 1180, 1189, 1410
BstMWI GCNNNNNNNGC 3 cut(s) 302, 314, 1232
BstNI CCWGG 3 cut(s) 90, 654, 662
BstNSI RCATGY 1 cut(s) 559
BstSCI CCNGG 3 cut(s) 88, 652, 660
BstV1I GCAGC 4 cut(s) 204, 280, 292, 1001
BstV2I GAAGAC 2 cut(s) 171, 734
BstX2I RGATCY 3 cut(s) 430, 976, 1180
BstYI RGATCY 3 cut(s) 430, 976, 1180
BtsCI GGATG 3 cut(s) 40, 65, 1336
BtsI GCAGTG 1 cut(s) 1131
BtsIMutI CAGTG 4 cut(s) 525, 873, 1093, 1131
Cfr13I GGNCC 2 cut(s) 656, 945
Csp6I GTAC 3 cut(s) 697, 1104, 1469
CviAII CATG 7 cut(s) 410, 556, 577, 1048, 1213, 1459, 1483
CviQI GTAC 3 cut(s) 697, 1104, 1469
DdeI CTNAG 9 cut(s) 221, 393, 489, 528, 534, 560, 777, 1110, 1278
DpnI GATC 8 cut(s) 414, 432, 581, 603, 978, 1182, 1191, 1412
DpnII GATC 8 cut(s) 412, 430, 579, 601, 976, 1180, 1189, 1410
Eam1104I CTCTTC 2 cut(s) 107, 276
EarI CTCTTC 2 cut(s) 107, 276
Eco130I CCWWGG 2 cut(s) 842, 941
Eco47I GGWCC 2 cut(s) 656, 945
Eco57I CTGAAG 1 cut(s) 946
EcoNI CCTNNNNNAGG 1 cut(s) 1006
EcoO109I RGGNCCY 1 cut(s) 656
EcoRII CCWGG 3 cut(s) 88, 652, 660
EcoT14I CCWWGG 2 cut(s) 842, 941
ErhI CCWWGG 2 cut(s) 842, 941
FaeI CATG 7 cut(s) 413, 559, 580, 1051, 1216, 1462, 1486
FaqI GGGAC 2 cut(s) 642, 1322
FatI CATG 7 cut(s) 409, 555, 576, 1047, 1212, 1458, 1482
FauNDI CATATG 1 cut(s) 985
FbaI TGATCA 1 cut(s) 412
Fnu4HI GCNGC 4 cut(s) 218, 294, 306, 990
FokI GGATG 3 cut(s) 47, 72, 1343
Fsp4HI GCNGC 4 cut(s) 218, 294, 306, 990
FspBI CTAG 6 cut(s) 30, 768, 843, 906, 1229, 1502
GluI GCNGC 4 cut(s) 218, 294, 306, 990
GsuI CTGGAG 4 cut(s) 185, 683, 819, 1450
HapII CCGG 2 cut(s) 158, 1178
Hin1II CATG 7 cut(s) 413, 559, 580, 1051, 1216, 1462, 1486
HincII GTYRAC 1 cut(s) 242
HindII GTYRAC 1 cut(s) 242
HinfI GANTC 9 cut(s) 253, 710, 838, 935, 1022, 1073, 1171, 1244, 1510
HpaII CCGG 2 cut(s) 158, 1178
HphI GGTGA 5 cut(s) 705, 710, 800, 1031, 1199
Hpy166II GTNNAC 3 cut(s) 242, 525, 890
Hpy188I TCNGA 8 cut(s) 75, 573, 616, 753, 837, 852, 1030, 1158
Hpy188III TCNNGA 8 cut(s) 257, 434, 798, 862, 964, 1193, 1429, 1502
Hpy8I GTNNAC 3 cut(s) 242, 525, 890
Hpy99I CGWCG 1 cut(s) 1449
HpyAV CCTTC 1 cut(s) 735
HpyCH4III ACNGT 4 cut(s) 147, 232, 378, 1088
HpyCH4IV ACGT 2 cut(s) 825, 1345
HpyCH4V TGCA 8 cut(s) 308, 340, 636, 884, 989, 1007, 1148, 1492
HpyF10VI GCNNNNNNNGC 3 cut(s) 302, 314, 1232
HpyF3I CTNAG 9 cut(s) 221, 393, 489, 528, 534, 560, 777, 1110, 1278
HpySE526I ACGT 2 cut(s) 825, 1345
Hsp92II CATG 7 cut(s) 413, 559, 580, 1051, 1216, 1462, 1486
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 8 cut(s) 412, 430, 579, 601, 976, 1180, 1189, 1410
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 7 cut(s) 160, 166, 214, 271, 406, 457, 1379
Lsp1109I GCAGC 4 cut(s) 204, 280, 292, 1001
LweI GCATC 1 cut(s) 856
MaeI CTAG 6 cut(s) 30, 768, 843, 906, 1229, 1502
MaeII ACGT 2 cut(s) 825, 1345
MaeIII GTNAC 1 cut(s) 1019
MalI GATC 8 cut(s) 414, 432, 581, 603, 978, 1182, 1191, 1412
MbiI CCGCTC 1 cut(s) 286
MboI GATC 8 cut(s) 412, 430, 579, 601, 976, 1180, 1189, 1410
MfeI CAATTG 1 cut(s) 12
MflI RGATCY 3 cut(s) 430, 976, 1180
MluCI AATT 7 cut(s) 12, 128, 198, 209, 368, 997, 1308
MlyI GAGTC 4 cut(s) 847, 1016, 1067, 1504
MmeI TCCRAC 1 cut(s) 972
MseI TTAA 3 cut(s) 381, 1122, 1404
MslI CAYNNNNRTG 3 cut(s) 1086, 1550, 1552
MspA1I CMGCKG 2 cut(s) 305, 992
MspI CCGG 2 cut(s) 158, 1178
MspR9I CCNGG 3 cut(s) 90, 654, 662
MunI CAATTG 1 cut(s) 12
Mva1269I GAATGC 2 cut(s) 295, 1382
MvaI CCWGG 3 cut(s) 90, 654, 662
MwoI GCNNNNNNNGC 3 cut(s) 302, 314, 1232
NdeI CATATG 1 cut(s) 985
NdeII GATC 8 cut(s) 412, 430, 579, 601, 976, 1180, 1189, 1410
NlaIII CATG 7 cut(s) 413, 559, 580, 1051, 1216, 1462, 1486
NlaIV GGNNCC 1 cut(s) 658
NmuCI GTSAC 1 cut(s) 1019
NspI RCATGY 1 cut(s) 559
OliI CACNNNNGTG 2 cut(s) 1550, 1552
PciSI GCTCTTC 1 cut(s) 276
PctI GAATGC 2 cut(s) 295, 1382
PfeI GAWTC 5 cut(s) 253, 710, 935, 1171, 1244
PflFI GACNNNGTC 1 cut(s) 1076
PkrI GCNGC 4 cut(s) 219, 295, 307, 991
PleI GAGTC 4 cut(s) 846, 1016, 1067, 1504
PpsI GAGTC 4 cut(s) 846, 1016, 1067, 1504
PpuMI RGGWCCY 1 cut(s) 656
PsiI TTATAA 1 cut(s) 498
Psp5II RGGWCCY 1 cut(s) 656
Psp6I CCWGG 3 cut(s) 88, 652, 660
PspGI CCWGG 3 cut(s) 88, 652, 660
PspN4I GGNNCC 1 cut(s) 658
PspPI GGNCC 2 cut(s) 656, 945
PspPPI RGGWCCY 1 cut(s) 656
PsrI GAACNNNNNNTAC 2 cut(s) 871, 903
PsuI RGATCY 3 cut(s) 430, 976, 1180
PsyI GACNNNGTC 1 cut(s) 1076
PvuII CAGCTG 2 cut(s) 305, 992
RsaI GTAC 3 cut(s) 698, 1105, 1470
RsaNI GTAC 3 cut(s) 697, 1104, 1469
RseI CAYNNNNRTG 3 cut(s) 1086, 1550, 1552
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 3 cut(s) 381, 1122, 1404
SatI GCNGC 4 cut(s) 218, 294, 306, 990
Sau3AI GATC 8 cut(s) 412, 430, 579, 601, 976, 1180, 1189, 1410
Sau96I GGNCC 2 cut(s) 656, 945
SchI GAGTC 4 cut(s) 847, 1016, 1067, 1504
ScrFI CCNGG 3 cut(s) 90, 654, 662
SfaNI GCATC 1 cut(s) 856
SinI GGWCC 2 cut(s) 656, 945
SmiMI CAYNNNNRTG 3 cut(s) 1086, 1550, 1552
SmlI CTYRAG 1 cut(s) 1540
SmoI CTYRAG 1 cut(s) 1540
Sse9I AATT 7 cut(s) 12, 128, 198, 209, 368, 997, 1308
SsiI CCGC 3 cut(s) 286, 385, 1360
SspMI CTAG 6 cut(s) 30, 768, 843, 906, 1229, 1502
StyD4I CCNGG 3 cut(s) 88, 652, 660
StyI CCWWGG 2 cut(s) 842, 941
TaaI ACNGT 4 cut(s) 147, 232, 378, 1088
TaiI ACGT 2 cut(s) 828, 1348
TaqI TCGA 8 cut(s) 251, 299, 582, 1169, 1202, 1268, 1447, 1516
TaqII GACCGA 1 cut(s) 93
TasI AATT 7 cut(s) 12, 128, 198, 209, 368, 997, 1308
TfiI GAWTC 5 cut(s) 253, 710, 935, 1171, 1244
Tru1I TTAA 3 cut(s) 381, 1122, 1404
Tru9I TTAA 3 cut(s) 381, 1122, 1404
TscAI CASTG 4 cut(s) 532, 880, 1093, 1138
TseFI GTSAC 1 cut(s) 1019
TseI GCWGC 4 cut(s) 217, 293, 305, 989
Tsp45I GTSAC 1 cut(s) 1019
TspDTI ATGAA 5 cut(s) 222, 294, 1064, 1381, 1475
TspGWI ACGGA 1 cut(s) 560
TspRI CASTG 4 cut(s) 532, 880, 1093, 1138
Tth111I GACNNNGTC 1 cut(s) 1076
VpaK11BI GGWCC 2 cut(s) 656, 945
XagI CCTNNNNNAGG 1 cut(s) 1006
XapI RAATTY 1 cut(s) 128
XbaI TCTAGA 1 cut(s) 1501
XceI RCATGY 1 cut(s) 559
XmaJI CCTAGG 1 cut(s) 842
XspI CTAG 6 cut(s) 30, 768, 843, 906, 1229, 1502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.